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The map label for this gene is cobS

Identifier: 147674142

GI number: 147674142

Start: 871030

End: 871815

Strand: Direct

Name: cobS

Synonym: VC0395_A0860

Alternate gene names: 147674142

Gene position: 871030-871815 (Clockwise)

Preceding gene: 147673919

Following gene: 147673396

Centisome position: 28.8

GC content: 50.0

Gene sequence:

>786_bases
ATGGCGGCCATTTTACGTTATCAACTGGAGCTGTTTTTGCTGGCCGTGAGCTTCTTCTCCCGCATTCCTGTGCCGGTGAG
TTTGCCTTATAGCAGTGAGCGAATGAATCAAGCTGGGCGCTATTTTGCTCTGGTTGGACTCCTGCTCGGGGCGATTTGCG
CTTTGGTTTATTCGCTCGCGACGCAGCTTTTTTCTACCAACATCAGCGTGTTTTTGACCATGGTGTTGAGCTTGTTATTG
ACCGGTGCCTTTCATGAAGACGGCTTAGCGGATATGGCTGACGGTGTGGGCGGTGGTATGACGGCTGAGCGACGTCTAGA
GATCATGAAAGACAGCCGCATTGGCACTTATGGCTCTAGCGCTTTGATTATGGTTTTGCTGGGTAAATATTTGCTACTCA
CCGAATTGGCCGATCTTACTTCACTGGTTCCTGTTTGGTTACTGGCTTATACCCTAAGCCGCGCGGTGGCGGCGTCTTTG
ATTCGTAATACGCCTTATGTCTCCGATACCGACAGCAGTAAAAGTAAGCCGCTGGCGCAGCAGCTGTCGGGTACGGATGT
GGCCGTGTTATCCCTTACCGCCTTAGCGACACTGCTTTACTTCTCATGGCAGTTTATTGGAGTGATGATCGCCGCCAGCT
TGATTTTCAGACAGATTTTCCGCCAATGGCTTATCAGACGTTTGGGTGGCTTTACTGGCGATTGCCTTGGCGCCGCACAG
CAGTTGATGGAAATTCTGATCTATCTGATCTTGCTTGCTTTTTTACAGCATGAAGTGATGATTTAG

Upstream 100 bases:

>100_bases
GTGAAGGTACCGGAGCTGTGTTGGCGCTGGGTCTTGTTCGTGCTGCAGTGGAGTTTTACAACCACATGGCCAGTTTTGCT
GATGCAGGAGTCACAGTGTA

Downstream 100 bases:

>100_bases
GGGGCAAAGTGACGGTTCATTTCGTTTTAGGTGGTGCCCGTAGTGGTAAATCGAGTTACGCAGAGTTACTCGCCAAACAA
ACTGCACAAGCTTTGGGGGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAAILRYQLELFLLAVSFFSRIPVPVSLPYSSERMNQAGRYFALVGLLLGAICALVYSLATQLFSTNISVFLTMVLSLLL
TGAFHEDGLADMADGVGGGMTAERRLEIMKDSRIGTYGSSALIMVLLGKYLLLTELADLTSLVPVWLLAYTLSRAVAASL
IRNTPYVSDTDSSKSKPLAQQLSGTDVAVLSLTALATLLYFSWQFIGVMIAASLIFRQIFRQWLIRRLGGFTGDCLGAAQ
QLMEILIYLILLAFLQHEVMI

Sequences:

>Translated_261_residues
MAAILRYQLELFLLAVSFFSRIPVPVSLPYSSERMNQAGRYFALVGLLLGAICALVYSLATQLFSTNISVFLTMVLSLLL
TGAFHEDGLADMADGVGGGMTAERRLEIMKDSRIGTYGSSALIMVLLGKYLLLTELADLTSLVPVWLLAYTLSRAVAASL
IRNTPYVSDTDSSKSKPLAQQLSGTDVAVLSLTALATLLYFSWQFIGVMIAASLIFRQIFRQWLIRRLGGFTGDCLGAAQ
QLMEILIYLILLAFLQHEVMI
>Mature_260_residues
AAILRYQLELFLLAVSFFSRIPVPVSLPYSSERMNQAGRYFALVGLLLGAICALVYSLATQLFSTNISVFLTMVLSLLLT
GAFHEDGLADMADGVGGGMTAERRLEIMKDSRIGTYGSSALIMVLLGKYLLLTELADLTSLVPVWLLAYTLSRAVAASLI
RNTPYVSDTDSSKSKPLAQQLSGTDVAVLSLTALATLLYFSWQFIGVMIAASLIFRQIFRQWLIRRLGGFTGDCLGAAQQ
LMEILIYLILLAFLQHEVMI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=36.6935483870968, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_VIBC3 (A5F1U5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F1U5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0860
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   PRK00235
- BioCyc:   VCHO345073:VC0395_A0860-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28593; Mature: 28462

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19483a14)-; HASH(0x186e4cb0)-; HASH(0x189b0d18)-; HASH(0x18b27e24)-; HASH(0x18b08660)-; HASH(0x18fd0ce4)-; HASH(0x18b4a62c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAILRYQLELFLLAVSFFSRIPVPVSLPYSSERMNQAGRYFALVGLLLGAICALVYSLA
CHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQLFSTNISVFLTMVLSLLLTGAFHEDGLADMADGVGGGMTAERRLEIMKDSRIGTYGSS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHH
ALIMVLLGKYLLLTELADLTSLVPVWLLAYTLSRAVAASLIRNTPYVSDTDSSKSKPLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QLSGTDVAVLSLTALATLLYFSWQFIGVMIAASLIFRQIFRQWLIRRLGGFTGDCLGAAQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QLMEILIYLILLAFLQHEVMI
HHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
AAILRYQLELFLLAVSFFSRIPVPVSLPYSSERMNQAGRYFALVGLLLGAICALVYSLA
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQLFSTNISVFLTMVLSLLLTGAFHEDGLADMADGVGGGMTAERRLEIMKDSRIGTYGSS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHH
ALIMVLLGKYLLLTELADLTSLVPVWLLAYTLSRAVAASLIRNTPYVSDTDSSKSKPLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QLSGTDVAVLSLTALATLLYFSWQFIGVMIAASLIFRQIFRQWLIRRLGGFTGDCLGAAQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QLMEILIYLILLAFLQHEVMI
HHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA