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The map label for this gene is priA [H]

Identifier: 147674045

GI number: 147674045

Start: 2399224

End: 2401428

Strand: Reverse

Name: priA [H]

Synonym: VC0395_A2251

Alternate gene names: 147674045

Gene position: 2401428-2399224 (Counterclockwise)

Preceding gene: 147674382

Following gene: 147674191

Centisome position: 79.41

GC content: 52.65

Gene sequence:

>2205_bases
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAGGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACA
CCGGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGGTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTCATGCACTCGGCGCTCAACGATACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGTGCTGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGTCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGCCATGAATGCGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAGATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAGCTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Upstream 100 bases:

>100_bases
AACCGATCGCGCCGATTATTTCACCAAAAGCGCGGGTCGAATTTCTGTCAAGCTTCCAGTAGACTGTGATCCGTTTGATT
TTTCCCTGTTTTGGACCCTG

Downstream 100 bases:

>100_bases
TTCACATCGCGTTTTATAACGCTGATGTGTGTTAATTTTGTCAGCAAATTAATGCTTATTATCGTGATGAAAATCACAGT
GACTATGCAAAATTTGTTAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Sequences:

>Translated_734_residues
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS
>Mature_734_residues
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=728, Percent_Identity=54.532967032967, Blast_Score=783, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82081; Mature: 82081

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQ
CCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHH
LKSVQAVLDDAPVWPDKLQSLLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHCCCCCCEE
LTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDEEVSSTTLKALVEKGWIECEE
EEECCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECC
RKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
CCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEHHHHH
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEE
VIGTRSALLTPFAKLGIIIVDEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATP
EEECHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
ALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYLESGLSAPLIAEMRRHLSAGN
HHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCC
QVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
EEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEECCCCCCCHHHHCCCCCCHHHHHCCC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGT
CCCEEEEEECCHHHHHHHHHHHCHHHHEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEH
QMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTH
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
HPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLFRAEANHTAQVEDFLRQVRET
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
HCCCCCCCCCCEEECCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
KVRWSIDIDPQDLS
CEEEEEECCCCCCC
>Mature Secondary Structure
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQ
CCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHH
LKSVQAVLDDAPVWPDKLQSLLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHCCCCCCEE
LTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDEEVSSTTLKALVEKGWIECEE
EEECCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECC
RKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
CCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEHHHHH
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEE
VIGTRSALLTPFAKLGIIIVDEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATP
EEECHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
ALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYLESGLSAPLIAEMRRHLSAGN
HHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCC
QVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
EEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEECCCCCCCHHHHCCCCCCHHHHHCCC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGT
CCCEEEEEECCHHHHHHHHHHHCHHHHEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEH
QMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTH
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
HPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLFRAEANHTAQVEDFLRQVRET
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
HCCCCCCCCCCEEECCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
KVRWSIDIDPQDLS
CEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]