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The map label for this gene is luxQ [H]

Identifier: 147674034

GI number: 147674034

Start: 1524594

End: 1526804

Strand: Reverse

Name: luxQ [H]

Synonym: VC0395_A1424

Alternate gene names: 147674034

Gene position: 1526804-1524594 (Counterclockwise)

Preceding gene: 147674242

Following gene: 147675599

Centisome position: 50.49

GC content: 45.05

Gene sequence:

>2211_bases
TTGGTTATGGCTATTCGCTCCTCGCTTAAAAAGAAAAGTATTCTAGCGCTGACCATTTATTTGGCCTTCTTCCTTGCGAT
TGTAGGAACGCTGAGTTATTGGGGGCTTGAAGTCCCATTTAGAAAAGAGCTGAAAAATAATCTAGCCCTGAGAGCGGAGT
TACTGGCTACCCAAATTCGTGAGCCATTGAATAACTCGATAGGGGTTCTGCAGAGTCTTACCAGCATTGGCAAAAGCGCA
GCCGATAAGGAAGAGCAAGAGCGCATGCTGCGCTCACTATTTTCTGTTGTTGGCGGAGTGATCATTAGCGGCGGGTTGTG
GCCGGAACCCAATCTATCGGCAACTGACCCCTCACTACGATATGACAGCTTGTTTTTTAATAAAGCAACCGATGGACAGG
TTGACCAACTGAGCTCATGGAATAACCCCAAAGCCGGGGGGTATGACCGAGAAAGCTGGTACTTGGCTGCAGAACGTGAA
GCCGAAGGGCTCTATTTCTGGTCACCTGTCTATGTTGACCCCTATACCCGTGTGGAAATGATCACGGTCTCGACCCCTTA
TTATCGTAACGGTCAGTTTGCGGGCGTTGCTACCGTTGATCTCTCACTTGAAAGCTTAATCCAATTCGTTGCTGCGACAG
CAGAGCAGTACAACTTAGGTGTGAATCTTAAAGATGCCTTTGGGGTTGAGGTCGTCTCACATAACTTCCGCACTTATGAT
AATGCGTTGGTCAGTTACTATAGTTTTGGCGAGTTCAATTGGCAGATTGAAGTGGTGAATGCTAATCAGCATGTCGATGA
AATCATCTTCGACTTAATTATCAACATCGAAAAAGGATTGATGCCGATCCTGCTGCTCTGTGTGATGGTGGGGTATTTTT
TAATTAGCCATTATTGGATCCGCCCAATTGTCTTAATTGCGAAAAAGGTGAGTGAGTCGAGAGAAGGTGAAATCATCGAT
ATTCGGTACAAATCTCAGGATGAGATCCGCCATCTTATTGATACGTTTAACCAAAAAACGATTTACTTAGAGGCAGAGAA
AGTCAAAGCTCAGGCCTCGACCAAAGCAAAAAGTGCATTTTTAGCCACGCTCTCTCATGAAATTCGAACGCCGATGAATG
GCGTGCTCGGTACCGCGCAAATCCTATTGAAAGATGAATTGACCTCTAAGCAGCGACAGCACTTAACCAGTCTCTACGAA
TCGGGCGAACATATGATGTCATTGCTCAATGAGATTTTAGATTATTCAAAAATAGAGCAAGGCAAATTCGAGTTGGATCA
CAGCGCATTTCCGCTCAAATCGATCATCGGCAGCATCAAAAGTATCTACTCCAGTCTTTGTGTTGAAAAAGGACTGAAAT
TCCAACTGAACTCAGAAATTACCGATGGACGATGGTACTACGGAGACAAGGCGCGTTTGCGGCAAATTATCTTCAATCTG
CTTAGTAATGCGGTGAAATTTACCGAAGCAGGCTTTGTCGCAATAGGGTTAAGTGAAGAAAGCTGTGACGAAGAGAATTA
CTTGATCATCAAAGTGCAGGATACGGGGATCGGCATCGCGCAAGAGTCGTTAGGGCGTATCTTTCGACCCTTTGAACAGG
CGGAATCACACACAACACGGCGTTTTGGTGGTACAGGCTTAGGCTTGGCTATTGTTAAACAGATTGCGGAATTAATGAAT
GGCACTGTTTTAGTGCAAAGTGAAGTTGGGCAGGGTTCTTGTTTTAAGGTGAGAGTTAAATTAGCGATTACCGAACCAGT
AACCGAAGATGTGAAACCTACTAAAGCCAAAACGTATCCCGGTTTACGGGTGCTGATTGTCGAAGATAACCGTACGAACA
TCATGATTCTGGAAGCTTTTATGCGCAATAAAGGTTTTGAGTGCCATAGCGTGATGGATGGTGTACAAGCGATTACCGCG
TTACAGGAAAGCTCATTTGATCTCGTTTTAATGGATAACCACATGCCGTTAAAAGATGGCATCCAAGCCACCAGAGAAAT
TCGTCAGTTGCCACTGCCGCAAGCCAAGATACTGTTGTTTGGTTGTACCGCAGATGTTTTCAAAGATACCCGGGATAAAA
TGCTCTCTGCAGGGGCAGATGACATTATCGCTAAACCCATTGCAGAGCATGAGCTAGATATGGCGCTTGAACAACACTCA
GAGCGCTTGTACCAGTTTCATCGCGAACCATCTTTACCATCGGTAGAGTAA

Upstream 100 bases:

>100_bases
TAAGAATGTTAGTTTGTGCACAGCGCCATGGAAAATCGATGAGAAAAGAAAGCCTGACAGAATAATTTCGCTATAATTTT
GTAAACAAAAAATAATTCAG

Downstream 100 bases:

>100_bases
ATCACTCTGTTTCTTCCTTCTTTCTTTCCTTTCTTATGCGTGTGGCCAAGTTCGGCTGGCAAGAAACTGTCTCACCGTGC
TCAGCGTGATGTAATAAAAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MVMAIRSSLKKKSILALTIYLAFFLAIVGTLSYWGLEVPFRKELKNNLALRAELLATQIREPLNNSIGVLQSLTSIGKSA
ADKEEQERMLRSLFSVVGGVIISGGLWPEPNLSATDPSLRYDSLFFNKATDGQVDQLSSWNNPKAGGYDRESWYLAAERE
AEGLYFWSPVYVDPYTRVEMITVSTPYYRNGQFAGVATVDLSLESLIQFVAATAEQYNLGVNLKDAFGVEVVSHNFRTYD
NALVSYYSFGEFNWQIEVVNANQHVDEIIFDLIINIEKGLMPILLLCVMVGYFLISHYWIRPIVLIAKKVSESREGEIID
IRYKSQDEIRHLIDTFNQKTIYLEAEKVKAQASTKAKSAFLATLSHEIRTPMNGVLGTAQILLKDELTSKQRQHLTSLYE
SGEHMMSLLNEILDYSKIEQGKFELDHSAFPLKSIIGSIKSIYSSLCVEKGLKFQLNSEITDGRWYYGDKARLRQIIFNL
LSNAVKFTEAGFVAIGLSEESCDEENYLIIKVQDTGIGIAQESLGRIFRPFEQAESHTTRRFGGTGLGLAIVKQIAELMN
GTVLVQSEVGQGSCFKVRVKLAITEPVTEDVKPTKAKTYPGLRVLIVEDNRTNIMILEAFMRNKGFECHSVMDGVQAITA
LQESSFDLVLMDNHMPLKDGIQATREIRQLPLPQAKILLFGCTADVFKDTRDKMLSAGADDIIAKPIAEHELDMALEQHS
ERLYQFHREPSLPSVE

Sequences:

>Translated_736_residues
MVMAIRSSLKKKSILALTIYLAFFLAIVGTLSYWGLEVPFRKELKNNLALRAELLATQIREPLNNSIGVLQSLTSIGKSA
ADKEEQERMLRSLFSVVGGVIISGGLWPEPNLSATDPSLRYDSLFFNKATDGQVDQLSSWNNPKAGGYDRESWYLAAERE
AEGLYFWSPVYVDPYTRVEMITVSTPYYRNGQFAGVATVDLSLESLIQFVAATAEQYNLGVNLKDAFGVEVVSHNFRTYD
NALVSYYSFGEFNWQIEVVNANQHVDEIIFDLIINIEKGLMPILLLCVMVGYFLISHYWIRPIVLIAKKVSESREGEIID
IRYKSQDEIRHLIDTFNQKTIYLEAEKVKAQASTKAKSAFLATLSHEIRTPMNGVLGTAQILLKDELTSKQRQHLTSLYE
SGEHMMSLLNEILDYSKIEQGKFELDHSAFPLKSIIGSIKSIYSSLCVEKGLKFQLNSEITDGRWYYGDKARLRQIIFNL
LSNAVKFTEAGFVAIGLSEESCDEENYLIIKVQDTGIGIAQESLGRIFRPFEQAESHTTRRFGGTGLGLAIVKQIAELMN
GTVLVQSEVGQGSCFKVRVKLAITEPVTEDVKPTKAKTYPGLRVLIVEDNRTNIMILEAFMRNKGFECHSVMDGVQAITA
LQESSFDLVLMDNHMPLKDGIQATREIRQLPLPQAKILLFGCTADVFKDTRDKMLSAGADDIIAKPIAEHELDMALEQHS
ERLYQFHREPSLPSVE
>Mature_736_residues
MVMAIRSSLKKKSILALTIYLAFFLAIVGTLSYWGLEVPFRKELKNNLALRAELLATQIREPLNNSIGVLQSLTSIGKSA
ADKEEQERMLRSLFSVVGGVIISGGLWPEPNLSATDPSLRYDSLFFNKATDGQVDQLSSWNNPKAGGYDRESWYLAAERE
AEGLYFWSPVYVDPYTRVEMITVSTPYYRNGQFAGVATVDLSLESLIQFVAATAEQYNLGVNLKDAFGVEVVSHNFRTYD
NALVSYYSFGEFNWQIEVVNANQHVDEIIFDLIINIEKGLMPILLLCVMVGYFLISHYWIRPIVLIAKKVSESREGEIID
IRYKSQDEIRHLIDTFNQKTIYLEAEKVKAQASTKAKSAFLATLSHEIRTPMNGVLGTAQILLKDELTSKQRQHLTSLYE
SGEHMMSLLNEILDYSKIEQGKFELDHSAFPLKSIIGSIKSIYSSLCVEKGLKFQLNSEITDGRWYYGDKARLRQIIFNL
LSNAVKFTEAGFVAIGLSEESCDEENYLIIKVQDTGIGIAQESLGRIFRPFEQAESHTTRRFGGTGLGLAIVKQIAELMN
GTVLVQSEVGQGSCFKVRVKLAITEPVTEDVKPTKAKTYPGLRVLIVEDNRTNIMILEAFMRNKGFECHSVMDGVQAITA
LQESSFDLVLMDNHMPLKDGIQATREIRQLPLPQAKILLFGCTADVFKDTRDKMLSAGADDIIAKPIAEHELDMALEQHS
ERLYQFHREPSLPSVE

Specific function: At low cell density, in absence of AI-2 (autoinducer 2), luxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=393, Percent_Identity=31.8066157760814, Blast_Score=216, Evalue=6e-57,
Organism=Escherichia coli, GI87081816, Length=368, Percent_Identity=36.1413043478261, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1788713, Length=403, Percent_Identity=32.5062034739454, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1789149, Length=290, Percent_Identity=34.8275862068965, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=37.5, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=30.6666666666667, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=25.6666666666667, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788393, Length=257, Percent_Identity=24.9027237354086, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786600, Length=252, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789808, Length=315, Percent_Identity=24.7619047619048, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=28.1938325991189, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790861, Length=287, Percent_Identity=24.0418118466899, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=238, Percent_Identity=27.3109243697479, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=27.6595744680851, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=207, Percent_Identity=26.5700483091787, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=194, Percent_Identity=30.4123711340206, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322000, Length=112, Percent_Identity=33.9285714285714, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 82711; Mature: 82711

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMAIRSSLKKKSILALTIYLAFFLAIVGTLSYWGLEVPFRKELKNNLALRAELLATQIR
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHH
EPLNNSIGVLQSLTSIGKSAADKEEQERMLRSLFSVVGGVIISGGLWPEPNLSATDPSLR
HHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCE
YDSLFFNKATDGQVDQLSSWNNPKAGGYDRESWYLAAEREAEGLYFWSPVYVDPYTRVEM
EHHEEECCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEECCCCEEEE
ITVSTPYYRNGQFAGVATVDLSLESLIQFVAATAEQYNLGVNLKDAFGVEVVSHNFRTYD
EEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHCCCHHHH
NALVSYYSFGEFNWQIEVVNANQHVDEIIFDLIINIEKGLMPILLLCVMVGYFLISHYWI
HHHHHHEEECCEEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RPIVLIAKKVSESREGEIIDIRYKSQDEIRHLIDTFNQKTIYLEAEKVKAQASTKAKSAF
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
LATLSHEIRTPMNGVLGTAQILLKDELTSKQRQHLTSLYESGEHMMSLLNEILDYSKIEQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
GKFELDHSAFPLKSIIGSIKSIYSSLCVEKGLKFQLNSEITDGRWYYGDKARLRQIIFNL
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCHHHHHHHHHHH
LSNAVKFTEAGFVAIGLSEESCDEENYLIIKVQDTGIGIAQESLGRIFRPFEQAESHTTR
HHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGGTGLGLAIVKQIAELMNGTVLVQSEVGQGSCFKVRVKLAITEPVTEDVKPTKAKTYP
HCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCC
GLRVLIVEDNRTNIMILEAFMRNKGFECHSVMDGVQAITALQESSFDLVLMDNHMPLKDG
CEEEEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
IQATREIRQLPLPQAKILLFGCTADVFKDTRDKMLSAGADDIIAKPIAEHELDMALEQHS
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERLYQFHREPSLPSVE
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVMAIRSSLKKKSILALTIYLAFFLAIVGTLSYWGLEVPFRKELKNNLALRAELLATQIR
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHH
EPLNNSIGVLQSLTSIGKSAADKEEQERMLRSLFSVVGGVIISGGLWPEPNLSATDPSLR
HHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCE
YDSLFFNKATDGQVDQLSSWNNPKAGGYDRESWYLAAEREAEGLYFWSPVYVDPYTRVEM
EHHEEECCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEECCCCEEEE
ITVSTPYYRNGQFAGVATVDLSLESLIQFVAATAEQYNLGVNLKDAFGVEVVSHNFRTYD
EEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHCCCHHHH
NALVSYYSFGEFNWQIEVVNANQHVDEIIFDLIINIEKGLMPILLLCVMVGYFLISHYWI
HHHHHHEEECCEEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RPIVLIAKKVSESREGEIIDIRYKSQDEIRHLIDTFNQKTIYLEAEKVKAQASTKAKSAF
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
LATLSHEIRTPMNGVLGTAQILLKDELTSKQRQHLTSLYESGEHMMSLLNEILDYSKIEQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
GKFELDHSAFPLKSIIGSIKSIYSSLCVEKGLKFQLNSEITDGRWYYGDKARLRQIIFNL
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCHHHHHHHHHHH
LSNAVKFTEAGFVAIGLSEESCDEENYLIIKVQDTGIGIAQESLGRIFRPFEQAESHTTR
HHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGGTGLGLAIVKQIAELMNGTVLVQSEVGQGSCFKVRVKLAITEPVTEDVKPTKAKTYP
HCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCC
GLRVLIVEDNRTNIMILEAFMRNKGFECHSVMDGVQAITALQESSFDLVLMDNHMPLKDG
CEEEEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
IQATREIRQLPLPQAKILLFGCTADVFKDTRDKMLSAGADDIIAKPIAEHELDMALEQHS
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERLYQFHREPSLPSVE
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]