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The map label for this gene is pdxB [H]

Identifier: 147673968

GI number: 147673968

Start: 1817897

End: 1819060

Strand: Reverse

Name: pdxB [H]

Synonym: VC0395_A1692

Alternate gene names: 147673968

Gene position: 1819060-1817897 (Counterclockwise)

Preceding gene: 147674185

Following gene: 147675308

Centisome position: 60.15

GC content: 49.91

Gene sequence:

>1164_bases
TTGCCGCAAATTCCCACTATGAAAATTCTGATTGATGAGAACATGCCTTACGCTCAAGCGCTGTTTAGTCAGCTTGGCGA
AGTGATCTTAAAACCCGGCCGTACCTTAACCGCCGATGATCTGATTGATGTCGATGCCTTAATGATTCGCTCAGTCACCA
AAGTCAATGAAGCTCTCCTTGCTAAAGCGAATCGCTTGAAGTTCGTGGGTACCGCGACCGCGGGTATGGACCATGTTGAC
CAAGCTCTGTTAAGAGAGCGTGGCATCTTCTTCACGGCGGCTCCTGGCTGTAACAAAGTCGGTGTTGCGGAATACGTGTT
CAGCGTGCTCATGGTGCTTGCGCAGCAGCAAGGGTTCTCGGTTTTCGATAAAACGGTAGGCATCATCGGTGCTGGCCAAG
TGGGTAGCTATCTTGCGAAGTGTCTCTCTGGCATTGGCATGAAAGTGCTGCTCAATGATCCGCCTAAGCAAGCGCAAGGG
GATGAGCGAGAATTTACCGAGCTGGAAACCTTACTTAAACAAGCAGATGTGATCACCCTGCATACGCCGATTACCCGCGA
CGGTGAGTGGCCAACCCATCATCTGATTGATGCGGCAATTCTTGAGCAACTGCGCAGTGACCAGATTTTAATTAATGCGG
CGCGTGGCCCTGTAGTGGATAACGCGGCGCTGAAAGCGCGTTTACAGAAAGGCGATGGTTTTACCGCTGTATTAGACGTG
TTTGAGTTTGAACCGCAAGTGGATATGGAATTGCTACCGCTGCTCGCATTTGCCACTCCGCATATTGCCGGTTATGGCCT
TGAAGGTAAGGCGCGTGGTACCACCATGATTTTCAATAGCTACTGCGAGTTTCTTGGCAGTGCACACTGTGCAAACCCAG
CAAGCTTGTTACCCAAAGCGCCTGTGCCCAAAGTTTATCTAGACCGAGCATGGGACGAGGAAACACTGCGTACGCTAACT
CAAATTATTTATGATGTGCGTAAAGATGACGCACAGTTTCGCCGCGAGATTCACCAACCCGGAGCGTTTGACTTAATGCG
CAAACACTATTGGGATAGACGTGAATACAGCGCGGTGACACTAGCTGGAGGCGCTGATTGTCATTTGGCTCCTTTGGCTA
AACTCGGTTTTCAAGTAGAGGTATGTGATGAGCCAACAATTTAA

Upstream 100 bases:

>100_bases
GGATCCTTTTGTTCTGGATGTTTGCCCGGCGGCCTAGGTCGTCGGGTATTTTTATTCTCGACAGCCTCCGTGCTTTTCTG
CACAATGCCTGCAACTTTGA

Downstream 100 bases:

>100_bases
TGTTGCCATTTTTGGCGCAACCGGCGCTGTAGGCGAAACCATGTTGGAAGTACTGCAGGAGCGTGAGTTTCCTGTGGATG
AACTTTTCCTGCTGGCCAGT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNEALLAKANRLKFVGTATAGMDHVD
QALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQG
DEREFTELETLLKQADVITLHTPITRDGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQKGDGFTAVLDV
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLDRAWDEETLRTLT
QIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI

Sequences:

>Translated_387_residues
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNEALLAKANRLKFVGTATAGMDHVD
QALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQG
DEREFTELETLLKQADVITLHTPITRDGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQKGDGFTAVLDV
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLDRAWDEETLRTLT
QIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI
>Mature_386_residues
PQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNEALLAKANRLKFVGTATAGMDHVDQ
ALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGD
EREFTELETLLKQADVITLHTPITRDGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQKGDGFTAVLDVF
EFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLDRAWDEETLRTLTQ
IIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=28.515625, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=28.515625, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=29.6875, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=30.078125, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=30.078125, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788660, Length=372, Percent_Identity=50.2688172043011, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=33.6, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787645, Length=209, Percent_Identity=28.2296650717703, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=29.8449612403101, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=310, Percent_Identity=30, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=352, Percent_Identity=27.8409090909091, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=352, Percent_Identity=27.8409090909091, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=277, Percent_Identity=25.2707581227437, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=272, Percent_Identity=27.5735294117647, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921140, Length=275, Percent_Identity=26.1818181818182, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574286, Length=237, Percent_Identity=28.6919831223629, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=26.6917293233083, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585514, Length=266, Percent_Identity=26.6917293233083, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574282, Length=266, Percent_Identity=26.6917293233083, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551003, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574284, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646446, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646448, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646452, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646450, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472511, Length=254, Percent_Identity=27.9527559055118, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 42592; Mature: 42460

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNEALL
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AKANRLKFVGTATAGMDHVDQALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFS
HHHHCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
VFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGDEREFTELETLLKQADVITL
HHHHHHCEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEE
HTPITRDGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQKGDGFTAVLDV
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKA
HHCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCC
PVPKVYLDRAWDEETLRTLTQIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVT
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCEEE
LAGGADCHLAPLAKLGFQVEVCDEPTI
EECCCCCCHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure 
PQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNEALL
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AKANRLKFVGTATAGMDHVDQALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFS
HHHHCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
VFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGDEREFTELETLLKQADVITL
HHHHHHCEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEE
HTPITRDGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQKGDGFTAVLDV
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKA
HHCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCC
PVPKVYLDRAWDEETLRTLTQIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVT
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCEEE
LAGGADCHLAPLAKLGFQVEVCDEPTI
EECCCCCCHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]