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The map label for this gene is cueR

Identifier: 147673719

GI number: 147673719

Start: 530618

End: 531037

Strand: Direct

Name: cueR

Synonym: VC0395_A0495

Alternate gene names: 147673719

Gene position: 530618-531037 (Clockwise)

Preceding gene: 147673133

Following gene: 147674558

Centisome position: 17.55

GC content: 49.05

Gene sequence:

>420_bases
ATGAATATTAGCCAGATTGCCAAACTCACCTCGCTGACTGCCAAGTCGATTCGTCTATACGAAGAGAAAGGTCTCATCAT
CCCACCACTGCGCTCTGAAAGTGGTTATCGCACTTACACTCAGCAGCATGTGGATGATTTACTGCTCATCGCTCGCTGCC
GGCGAGTCGGTTTTAGTTTGGATGAGTGTAAAGCCATGCTCACTCTTGCCAATGATCCAAACCGAACGAGCGCAGCGGTG
CGCGCGCGAGCGCAAGAAAAGTGGCAAGAAATCTCGAGAAAACTCTCCGAGCTCACCATGATTAAGCAGCAGCTAGAAGA
GTGGATTGCATCTTGTCCCGGAGATCAAGGCAGCGATTGCCCAATCATAGAACAGTTGAAGGGACATTGTTGTTCAAACA
ATAAAACTAAGACCCCGTAG

Upstream 100 bases:

>100_bases
AAAAGCATCGATTCTCTCTTGGTTGATTAGCCAAGAATAATTGCATTTCTCGCTGGATAGAGGCATGGTCTTTCCATGCC
TCTGTGATTTTTTATGTGTC

Downstream 100 bases:

>100_bases
GATCTTAGTTCCGACTTGAAGTTGCCTACAGAATGATAGACTTTTAGATAGGTTGGACGACTAAGATAATCCCATCCACA
GCAACGACAGAAACACGGCT

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: Copper efflux regulator; Copper export regulator

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MNISQIAKLTSLTAKSIRLYEEKGLIIPPLRSESGYRTYTQQHVDDLLLIARCRRVGFSLDECKAMLTLANDPNRTSAAV
RARAQEKWQEISRKLSELTMIKQQLEEWIASCPGDQGSDCPIIEQLKGHCCSNNKTKTP

Sequences:

>Translated_139_residues
MNISQIAKLTSLTAKSIRLYEEKGLIIPPLRSESGYRTYTQQHVDDLLLIARCRRVGFSLDECKAMLTLANDPNRTSAAV
RARAQEKWQEISRKLSELTMIKQQLEEWIASCPGDQGSDCPIIEQLKGHCCSNNKTKTP
>Mature_139_residues
MNISQIAKLTSLTAKSIRLYEEKGLIIPPLRSESGYRTYTQQHVDDLLLIARCRRVGFSLDECKAMLTLANDPNRTSAAV
RARAQEKWQEISRKLSELTMIKQQLEEWIASCPGDQGSDCPIIEQLKGHCCSNNKTKTP

Specific function: Regulates the transcription of the copA and cueO genes. It detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations

COG id: COG0789

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH merR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786695, Length=133, Percent_Identity=54.1353383458647, Blast_Score=155, Evalue=7e-40,
Organism=Escherichia coli, GI1789687, Length=128, Percent_Identity=30.46875, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CUER_VIBC3 (A5F2W6)

Other databases:

- EMBL:   AJ277893
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2W6
- SMR:   A5F2W6
- STRING:   A5F2W6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0495
- eggNOG:   COG0789
- HOGENOM:   HBG710951
- OMA:   TRTHLAY
- ProtClustDB:   CLSK793890
- BioCyc:   VCHO345073:VC0395_A0495-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011789
- InterPro:   IPR009061
- InterPro:   IPR000551
- InterPro:   IPR015358
- SMART:   SM00422
- TIGRFAMs:   TIGR02044

Pfam domain/function: PF00376 MerR; PF09278 MerR-DNA-bind; SSF46955 Putativ_DNA_bind

EC number: NA

Molecular weight: Translated: 15693; Mature: 15693

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00552 HTH_MERR_1; PS50937 HTH_MERR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISQIAKLTSLTAKSIRLYEEKGLIIPPLRSESGYRTYTQQHVDDLLLIARCRRVGFSL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
DECKAMLTLANDPNRTSAAVRARAQEKWQEISRKLSELTMIKQQLEEWIASCPGDQGSDC
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIIEQLKGHCCSNNKTKTP
CHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNISQIAKLTSLTAKSIRLYEEKGLIIPPLRSESGYRTYTQQHVDDLLLIARCRRVGFSL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
DECKAMLTLANDPNRTSAAVRARAQEKWQEISRKLSELTMIKQQLEEWIASCPGDQGSDC
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIIEQLKGHCCSNNKTKTP
CHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA