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The map label for this gene is yafJ [H]

Identifier: 147673553

GI number: 147673553

Start: 1935707

End: 1936552

Strand: Reverse

Name: yafJ [H]

Synonym: VC0395_A1821

Alternate gene names: 147673553

Gene position: 1936552-1935707 (Counterclockwise)

Preceding gene: 147673432

Following gene: 147675197

Centisome position: 64.04

GC content: 50.12

Gene sequence:

>846_bases
ATGTGTGAATTGCTCGGCATGAGCGCCAATGTACCAACCGATATCTGTTTTAGTTTCACTGGCTTGATGCAACGTGGCGG
GAAAACTGGTCCGCATCGTGATGGGTGGGGAATCGCGTTTTACGAAGGCAAAGGCTTTCGCACCTTCAAAGATCCTAATC
CCAGCAGTCAGTCCAAAATTGCCGAGTTAGTTCAGCAGTATCCGATCAAAAGTCGTGCGGTGATCAGCCACATTCGTCAG
GCCAATCGCGGTGGGGTGAATCTGGAAAACACTCATCCGTTTACCCGCGAGTTGTGGGGGCAGTACTGGACGTTTGCCCA
CAATGGGCAATTGACGGGATATCAAAATCTAGTCACTGGACGCCATCGACCAGTCGGTGAAACGGACAGCGAACTGGCGT
TCTGTTGGTTGCTGAATCAAATGGAACAGTGTTACCCCGAAGGTCCACAAGATTGGCCTGAGATGCTGCGCTATGTTGCG
CAGTGTTGTGATGAGCTTAGAGCTCTCGGTGTCTTTAATATGCTGCTGTCTAATGGCGAATATGTGATGGCGTATTGCAC
CAATCATTTGTATTGGATTACTCGCCGAGCCCCATTTGGCTGCGCGGCACTGCTCGATGAAGATGTCGAGATCAATTTCC
AAGAAGAAACCACGCCGAATGATGTGGTGTCAGTGATTGCGACTCAACCGTTAACCGGTAATGAAACTTGGCAGCGTATG
AAGCCGGGGCAATTCGCATTTTTCCATTTTGGAGAGCGGATAGAAGACAATGCACATGTGTTGATGGATGTGGGTTTTGC
ACCGAATCGACCCGGATGTCAGGCACCAAGCCAACCTCTCGACTAA

Upstream 100 bases:

>100_bases
ACATCATCATTCAGTTGATTGAAAAGGAAATGGCGTAACCCTACGCTCAAGAGCCCGGTATTAACCGGGCTACTGGTTTA
AGTAAGGAGTGGTTTAAACC

Downstream 100 bases:

>100_bases
GCGATATACCTTTCCTACTTGAAGCTGCAGCGGTGTTGGCTATGTTCGTTCACCCCAATCACATAGTTTATGTTGTCTGG
GAATAAAAAGTGGTACAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFYEGKGFRTFKDPNPSSQSKIAELVQQYPIKSRAVISHIRQ
ANRGGVNLENTHPFTRELWGQYWTFAHNGQLTGYQNLVTGRHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVA
QCCDELRALGVFNMLLSNGEYVMAYCTNHLYWITRRAPFGCAALLDEDVEINFQEETTPNDVVSVIATQPLTGNETWQRM
KPGQFAFFHFGERIEDNAHVLMDVGFAPNRPGCQAPSQPLD

Sequences:

>Translated_281_residues
MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFYEGKGFRTFKDPNPSSQSKIAELVQQYPIKSRAVISHIRQ
ANRGGVNLENTHPFTRELWGQYWTFAHNGQLTGYQNLVTGRHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVA
QCCDELRALGVFNMLLSNGEYVMAYCTNHLYWITRRAPFGCAALLDEDVEINFQEETTPNDVVSVIATQPLTGNETWQRM
KPGQFAFFHFGERIEDNAHVLMDVGFAPNRPGCQAPSQPLD
>Mature_281_residues
MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFYEGKGFRTFKDPNPSSQSKIAELVQQYPIKSRAVISHIRQ
ANRGGVNLENTHPFTRELWGQYWTFAHNGQLTGYQNLVTGRHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVA
QCCDELRALGVFNMLLSNGEYVMAYCTNHLYWITRRAPFGCAALLDEDVEINFQEETTPNDVVSVIATQPLTGNETWQRM
KPGQFAFFHFGERIEDNAHVLMDVGFAPNRPGCQAPSQPLD

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=72.0472440944882, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 31824; Mature: 31824

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFYEGKGFRTFKDPNPSSQSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AELVQQYPIKSRAVISHIRQANRGGVNLENTHPFTRELWGQYWTFAHNGQLTGYQNLVTG
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHCC
RHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVAQCCDELRALGVFNMLLSNGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMAYCTNHLYWITRRAPFGCAALLDEDVEINFQEETTPNDVVSVIATQPLTGNETWQRM
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHC
KPGQFAFFHFGERIEDNAHVLMDVGFAPNRPGCQAPSQPLD
CCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFYEGKGFRTFKDPNPSSQSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AELVQQYPIKSRAVISHIRQANRGGVNLENTHPFTRELWGQYWTFAHNGQLTGYQNLVTG
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHCC
RHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVAQCCDELRALGVFNMLLSNGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMAYCTNHLYWITRRAPFGCAALLDEDVEINFQEETTPNDVVSVIATQPLTGNETWQRM
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHC
KPGQFAFFHFGERIEDNAHVLMDVGFAPNRPGCQAPSQPLD
CCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]