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The map label for this gene is purT [H]

Identifier: 147673455

GI number: 147673455

Start: 861168

End: 862349

Strand: Reverse

Name: purT [H]

Synonym: VC0395_A0850

Alternate gene names: 147673455

Gene position: 862349-861168 (Counterclockwise)

Preceding gene: 147674190

Following gene: 147675016

Centisome position: 28.52

GC content: 52.03

Gene sequence:

>1182_bases
TTGTCTATGTTTGGGACAGCGACTCGGGATAACGCAACGCGAGTACTTTTGCTTGGCTCTGGTGAACTTGGTAAGGAAGT
CGCGATTGAGTGTCAGCGCCTTGGCCTCGAAGTCATCGCCTGTGATCGTTACGCCGAGGCGCCTGCCATGCAGGTCGCCC
ATCGCAGCCACGTGTTTGATATGCTCGATGCCGATGCACTACAACAAGTGATTGATTTAGAAAAGCCACATTTCGTGGTT
CCAGAGATTGAAGCGATTGCCACCAGCAAGTTAGTGGAGTTAGAAGCGCAAGGCTTAAACGTTGTGCCTACCGCCAATGC
AACTCGCCTCACCATGAACCGTGAAGGCATTCGCCGTTTAGCCGCTGAAGAGTTAGGGTTGCCCACTTCCGCGTATCAAT
TCGCAGACAGCTATGAGAGCTTTGCCGCGGCAGTAGAAACCATCGGTTTACCTTGTGTCTGTAAGCCTGTCATGAGTTCA
TCGGGCAAAGGCCAAAGTGTTATTCGCACACCAGAGCAGATCGAAGCGGCTTGGCAATACGCACAACAAGGTGGACGCAG
TGGCGCTGGCCGAGTGATTGTGGAAGGCTTCGTGGATTTTGATTACGAAATCACACTGCTGACCGTACGCGCTGTGGATG
GCGTCCATTTCTGCGCTCCAATTGGCCATCGCCAAGAAGATGGCGATTACCGCGAGTCATGGCAACCACAACTGATGTCA
GAAAATGCCATTAAAGCGGCTGAATATGTGGCAGAACAGGTGGTTAACGCGCTTGGTGGTTATGGCTTGTTTGGAGTTGA
ACTGTTTGTGAAAGGTGACAAGGTCATCTTCAACGAGGTTTCCCCTCGTCCACACGATACCGGAATGGTGACGCTCATTT
CTCAAGAGCTCTCTGAATTCGCTTTGCATGTGCGCGCGTTTACTGGTCTACCGATTGGTCAAATCGTGCAATATGGCCCA
TCAGCCTCTGCGGCGATCCTCGGTCAAGGCCAATCACAGAACATCCAGTTCAGTGCGCTTGATGACGCCCTAAGCATTCC
TCACACCCAAGTGCGCTTATTTGGCAAGCCAGATATTGCGGGTCGTCGTCGCTTAGGTGTTGCCCTGAGCCGAGGAAAAA
CGACGCAAGAAGCCACTGATCGCGCGATTGAGTGTGCGAAAGCCGTGAAGATTCATTACTAA

Upstream 100 bases:

>100_bases
ATCTGACTGGCTACAGCGGGAAGCAAACGTTTTCTTTTGTGCTTTGTTCCAGTATCATGGCCGCTGTTTTTTGCTAATGT
TGACTGAATGACTATAAGGA

Downstream 100 bases:

>100_bases
TTCAGCTCTTTATCTTTATCAGCACTATCAGAAAGCGCGCTCATTCAGCGCGCTTTTTTCATTTCTACTTAGTGGCGTTC
AAAGAGTCAGAGTCACTCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYAEAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVV
PEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSS
SGKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQLMS
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGP
SASAAILGQGQSQNIQFSALDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY

Sequences:

>Translated_393_residues
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYAEAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVV
PEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSS
SGKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQLMS
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGP
SASAAILGQGQSQNIQFSALDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
>Mature_392_residues
SMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYAEAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVVP
EIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSS
GKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQLMSE
NAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGPS
ASAAILGQGQSQNIQFSALDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=70.6632653061224, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI1786733, Length=256, Percent_Identity=26.5625, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=24.4505494505494, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42591; Mature: 42460

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYAEAPAMQVAHRSHVFD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDADALQQVIDLEKPHFVVPEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHEEECCCCEEEECCCCEEEEECHHHHHHH
AAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSSGKGQSVIRTPEQIEAAWQY
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCEECCHHHHHHHHHH
AQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQLMS
HHHCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCHHHC
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFTGLPIGQIVQYGPSASAAILGQGQSQNIQFSALDDALSIPHTQVRLFGKPDIA
HHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEEEECCHHHCCCCEEEEEECCCCCC
GRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
CCHHHCEEEECCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYAEAPAMQVAHRSHVFD
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDADALQQVIDLEKPHFVVPEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHEEECCCCEEEECCCCEEEEECHHHHHHH
AAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSSGKGQSVIRTPEQIEAAWQY
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCEECCHHHHHHHHHH
AQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQLMS
HHHCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCHHHC
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFTGLPIGQIVQYGPSASAAILGQGQSQNIQFSALDDALSIPHTQVRLFGKPDIA
HHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEEEECCHHHCCCCEEEEEECCCCCC
GRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
CCHHHCEEEECCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA