Click here to switch to the map view.

The map label for this gene is hupB

Identifier: 147673385

GI number: 147673385

Start: 1622013

End: 1622285

Strand: Reverse

Name: hupB

Synonym: VC0395_A1509

Alternate gene names: 147673385

Gene position: 1622285-1622013 (Counterclockwise)

Preceding gene: 147673301

Following gene: 147674490

Centisome position: 53.65

GC content: 48.72

Gene sequence:

>273_bases
GTGAATAAGACACAACTAGTAGAACAAATTGCAGCGAACGCAGATATCTCTAAAGCCTCTGCAGGTCGCGCTCTTGACGC
ATTCATCGAAGCAGTCAGCGGCACACTACAATCAGGTGACCAAGTGGCACTGGTTGGTTTTGGTACGTTCAGCGTACGTA
CTCGCGCAGCGCGTACTGGTCGTAACCCAAAAACTGGCGAAGAGATCAAAATCGCTGAAGCGAAAGTGCCTTCATTCAAA
GCAGGTAAAGCTCTGAAAGACGCTTGCAACTAA

Upstream 100 bases:

>100_bases
TTCTAGCCCTGATGCAACAAGGCTTTAGGCTAGTTTTAAACTTTAATGGAACGAAATCGGCACCGATAAAATAATAAAGG
GTGACCAAAGGGGAATCACA

Downstream 100 bases:

>100_bases
TTTGAATGAAGATTTTGCACTTTTCTGCAAAATAAATTCGAACCTTTTGAAATTATGCGCATCCTACTGATGCGCATTTC
TTTTTCTGATATTATCGCGC

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKTQLVEQIAANADISKASAGRALDAFIEAVSGTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIKIAEAKVPSFK
AGKALKDACN

Sequences:

>Translated_90_residues
MNKTQLVEQIAANADISKASAGRALDAFIEAVSGTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIKIAEAKVPSFK
AGKALKDACN
>Mature_90_residues
MNKTQLVEQIAANADISKASAGRALDAFIEAVSGTLQSGDQVALVGFGTFSVRTRAARTGRNPKTGEEIKIAEAKVPSFK
AGKALKDACN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=73.3333333333333, Blast_Score=135, Evalue=4e-34,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=59.5505617977528, Blast_Score=107, Evalue=2e-25,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=39.5604395604396, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1788005, Length=86, Percent_Identity=38.3720930232558, Blast_Score=71, Evalue=1e-14,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): DBHB_VIBCH (Q9KQS9)

Other databases:

- EMBL:   AE003852
- PIR:   F82141
- RefSeq:   NP_231553.1
- ProteinModelPortal:   Q9KQS9
- SMR:   Q9KQS9
- GeneID:   2613548
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1919
- TIGR:   VC_1919
- HOGENOM:   HBG705085
- OMA:   NESKTHF
- ProtClustDB:   CLSK874600
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 9366; Mature: 9366

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTQLVEQIAANADISKASAGRALDAFIEAVSGTLQSGDQVALVGFGTFSVRTRAARTG
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
RNPKTGEEIKIAEAKVPSFKAGKALKDACN
CCCCCCCEEEEECCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MNKTQLVEQIAANADISKASAGRALDAFIEAVSGTLQSGDQVALVGFGTFSVRTRAARTG
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
RNPKTGEEIKIAEAKVPSFKAGKALKDACN
CCCCCCCEEEEECCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301