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The map label for this gene is purD [H]

Identifier: 147673352

GI number: 147673352

Start: 2804679

End: 2805968

Strand: Reverse

Name: purD [H]

Synonym: VC0395_A2652

Alternate gene names: 147673352

Gene position: 2805968-2804679 (Counterclockwise)

Preceding gene: 147673909

Following gene: 147674782

Centisome position: 92.79

GC content: 54.65

Gene sequence:

>1290_bases
ATGCAAGTTCTCATCATCGGCTCTGGCGGTCGCGAACACGCACTGGCGTGGAAAGTGGCACAAAACCCACAAGTTGACAC
TATTTACGTCGCGCCCGGCAATGCGGGTACTGCCCTTGAGCACAAGGTGCAAAACGTCAACATTGGCATCACCGACATTC
CAGCACTGGTTGCCTTTGCTCAAGATAAAGCGATTGAGCTGACCATCGTTGGCCCAGAAGCACCACTGGTGATTGGTGTG
GTCGATGCGTTTCGCGCTGCAGGTTTACCGATTTTTGGCCCCACCCAAGGCGCGGCGCAACTAGAAGGCTCCAAAGCCTT
CACTAAAGATTTCCTCGCTCGCCATCACATTCCAACAGCGGCTTACGCCAACTTCACTGAAATTGAGCCAGCTCTGGCTT
ATGTGCGTAAAAAAGGTGCGCCGATTGTCGTGAAAGCCGACGGTTTAGCGGCGGGCAAAGGCGTAATTGTTGCGATGACT
CTGCAAGAGGCCGAAAACGCAATTCAAGACATGCTAGCTGGCAATGCCTTTGGTTCAGCAGGCAGCCGTGTGGTGGTTGA
AGAATTCCTTGATGGAGAGGAAGCGAGCTTTATCGTGATGGTTGATGGTGAAAACGTCCTGCCTATGGCCACCAGCCAAG
ATCACAAACGCGTGGGCGATGCCGATACTGGCCCGAACACCGGCGGTATGGGTGCTTACTCACCAGCGCCAGTGGTGACA
CAGGACGTCCATGATCGCGTAATGCGTGAAGTGATTTACCCAACCGTGCGCGGCATGGCAGCCGAAGGCAACACCTACAC
AGGCTTCCTCTACGCGGGTTTGATGATAGATAGCACTGGTGCACCGAAAGTGATTGAGTACAACTGCCGCTTTGGTGACC
CAGAAACCCAACCGATCATGATGCGTCTGCAATCGGATCTGGTTGAGCTGTGCCAAGCAGCAATTGCGGGCAAACTGGAT
CAAGTGGAATCGAAGTGGGATCCACGCGCCTCGATCGGTGTGGTTCTCGCCGCTGGCGGCTACCCTGGCGATTACACCAA
AGGTGAGGTGATTTCAGGTCTACCGACTCAAGAAAGCGCAGGACAAAAAGTGTTCCACGCAGGCACCGAAACCCAAGGCG
ACCAAGTGGTTACGAATGGCGGTCGCGTATTGTGTGCCACGGCGCTGGGCAATACCGTATTAGAAGCGCAGCAACGCGCT
TACCAACTGGCGGATCAAATCCACTGGAATGGTATGTTCTGCCGCCGCGATATCGGTTATCGCGCGATTGCGCGTGAGCA
AGCCAAGTAA

Upstream 100 bases:

>100_bases
ATGGCGATGATCTTCACCGGTATGCGTCACTTCCGTCATTAATTGGCATGATTGGTATGGGGTGCAACGCGCCCCATCTT
GTTTAAGTAAGGATTTCACA

Downstream 100 bases:

>100_bases
GCGGGCATCCATCTTCTTATCAATAGAAAAAAGCGCGAACTTCGCGCTTTTTTATTCGATAGGTTTTTTATTCGATTAGA
TTGGCAAACATGGGCCGATT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHHIPTAAYANFTEIEPALAYVRKKGAPIVVKADGLAAGKGVIVAMT
LQEAENAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIYPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYTKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK

Sequences:

>Translated_429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHHIPTAAYANFTEIEPALAYVRKKGAPIVVKADGLAAGKGVIVAMT
LQEAENAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIYPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYTKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK
>Mature_429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHHIPTAAYANFTEIEPALAYVRKKGAPIVVKADGLAAGKGVIVAMT
LQEAENAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIYPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYTKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=50.7009345794392, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=50.7009345794392, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=50.7009345794392, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=50.7009345794392, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=78.0373831775701, Blast_Score=695, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=41.3145539906103, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=41.2162162162162, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.8139534883721, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.8139534883721, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45408; Mature: 45408

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHEEEECCCHHHHHHHHHH
QDKAIELTIVGPEAPLVIGVVDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHHIPTA
CCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
AYANFTEIEPALAYVRKKGAPIVVKADGLAAGKGVIVAMTLQEAENAIQDMLAGNAFGSA
HHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
CCEEHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
QDVHDRVMREVIYPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHH
MRLQSDLVELCQAAIAGKLDQVESKWDPRASIGVVLAAGGYPGDYTKGEVISGLPTQESA
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRAYQLADQIHWNGMFCRRDIGY
CCEEEECCCCCCCCEEEECCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
RAIAREQAK
HHHHHHCCC
>Mature Secondary Structure
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHEEEECCCHHHHHHHHHH
QDKAIELTIVGPEAPLVIGVVDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHHIPTA
CCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
AYANFTEIEPALAYVRKKGAPIVVKADGLAAGKGVIVAMTLQEAENAIQDMLAGNAFGSA
HHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
CCEEHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
QDVHDRVMREVIYPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHH
MRLQSDLVELCQAAIAGKLDQVESKWDPRASIGVVLAAGGYPGDYTKGEVISGLPTQESA
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRAYQLADQIHWNGMFCRRDIGY
CCEEEECCCCCCCCEEEECCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
RAIAREQAK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]