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The map label for this gene is tcpT [H]

Identifier: 147673300

GI number: 147673300

Start: 386895

End: 388406

Strand: Direct

Name: tcpT [H]

Synonym: VC0395_A0360

Alternate gene names: 147673300

Gene position: 386895-388406 (Clockwise)

Preceding gene: 147673398

Following gene: 147675450

Centisome position: 12.79

GC content: 36.11

Gene sequence:

>1512_bases
ATGTCAATTGATATAAAGTATTTAAGTCGTATTGATATAGATCGTGAGGAGTTTTTCTTTAAAGACTCGAGATTAATGTG
TAAGAAATTCGATGAAGAGCGTGAGGTATTAACTCTTCTCGAATTCGATACAAAATTTCGGGTCAATCTTCTGAAGAAAG
ATAAAGTTTATAAGTATTTCTTAGTTTCAGATGCAAATCATAAATTATTAATAGCGAATCTTGTTACCGAGCAGCAAGCT
AAAGACTTAAGTTTTATAGAAAAAGATATAATGAAAATTGCATCATCTGCTACAGCTTATGGTGCAAGTGACATCCACTT
CATCCGAGAGGATAGGATTTGTAAAATAAAATTCCGTGTGAATGGCACAATGATCGACTATAGAGAAATATTAAGTTCAG
AAGCCGATTCGCTAATGTTTGTTCTATACAATGTGATGGCAACAACGAAAGAAACGACGTGGAACCGAAAACTACCTCAA
GATGCAAATATTATTCTTGTGATCAACGAAAAGGCATATCGCTTTCGTTACGCACATATGCCTTTATTTGGTGAGGGAGG
GAAAAACTATCATGCGGTAGTTCGTATTATCTATCCTTCAAATAATTTTGTTTGTACAAACTATCAAGATATTGGGTATA
ACGAGGCTGATACCGATGCGATTGCAAGAATTCTAAATACATCTTATGGATTGTTCATTGTTTCCGGAACAACAGGTTCT
GGTAAATCAACATCTTTGAAAAAGTATATAGAATTACTTTTCTTTAATAAATATAAGGGAAAAGGATGTTTTGTCACCGT
TGAAGATCCTGTCGAGTATTTAATTTCTGGAGCACAGCAAAGCTCAATTGTTGCCGATAATGATGATAAAACAAAGAACC
CATTTGCTGATGCGGTACGTTCAGCGATGCGTCGAGATCCGGACGTTATTATGATCGGTGAAATTCGTGATAAACCTACC
GTTGAGGCTCTATCAAGTGCTGTAGAAAGTGGCCACTATTGTCTTACGACCATCCATGCAGGTTCTGTTGTTTCTGTTTT
ACAAAGACTTTCAGGATTAGGGATGAAGGCTGACAAAATAGCATCACCTGGATTTCTGGCTGGTATTACAAGTCAGAAAT
TAATTCCCGAACTTTGCCCAAGTTGTAAAGTTTCCTTTGTTGATGAACGTTATCAGCGTGCTGTATTTAGCGCGAATGAA
AATGGCTGTGAGGCATGTAATCATTCTGGGTTTAAAGGAAGGCTGCTTTTATTAGAAACATTAGTCCCAACAGTTGAAGA
TTTAGAGCTAGTGGCGAGTGAAAACTGGGTTTCCCTTTATCGTAAATATCGGGAGCGACGCTTTATAAAAACTGGAAAAA
AAGGTCTTGGTGAAGGCTTCAGCATTAAAGATAAAGCTTATTACAATGTTTTAAAAGGTAAAGTGTGTCACGAATATTTT
ATGCTGCATTTCGGTCAGTTAGATCATGAGGATGAAAATATAATATATGAAAATTATCTCCAAGAAGTATAG

Upstream 100 bases:

>100_bases
GGCAAACTTATCACATTGGTTAATGGTGATTTTGTAAATGGTTGGAAATTCATTGAAGACCAAAATAAAATTCAATTTTT
CAAGGGCAATAAGGTAATCG

Downstream 100 bases:

>100_bases
GCTAGAACTTTATTCAATGCTTGTGGACTTATTGAATGATAATATCCCACTCTATGATGCATTAAATAAAATACAAAATG
AGGGAGTGGGCATCTATGAT

Product: toxin co-regulated pilus biosynthesis protein T

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpT [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDTKFRVNLLKKDKVYKYFLVSDANHKLLIANLVTEQQA
KDLSFIEKDIMKIASSATAYGASDIHFIREDRICKIKFRVNGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQ
DANIILVINEKAYRFRYAHMPLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADTDAIARILNTSYGLFIVSGTTGS
GKSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLISGAQQSSIVADNDDKTKNPFADAVRSAMRRDPDVIMIGEIRDKPT
VEALSSAVESGHYCLTTIHAGSVVSVLQRLSGLGMKADKIASPGFLAGITSQKLIPELCPSCKVSFVDERYQRAVFSANE
NGCEACNHSGFKGRLLLLETLVPTVEDLELVASENWVSLYRKYRERRFIKTGKKGLGEGFSIKDKAYYNVLKGKVCHEYF
MLHFGQLDHEDENIIYENYLQEV

Sequences:

>Translated_503_residues
MSIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDTKFRVNLLKKDKVYKYFLVSDANHKLLIANLVTEQQA
KDLSFIEKDIMKIASSATAYGASDIHFIREDRICKIKFRVNGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQ
DANIILVINEKAYRFRYAHMPLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADTDAIARILNTSYGLFIVSGTTGS
GKSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLISGAQQSSIVADNDDKTKNPFADAVRSAMRRDPDVIMIGEIRDKPT
VEALSSAVESGHYCLTTIHAGSVVSVLQRLSGLGMKADKIASPGFLAGITSQKLIPELCPSCKVSFVDERYQRAVFSANE
NGCEACNHSGFKGRLLLLETLVPTVEDLELVASENWVSLYRKYRERRFIKTGKKGLGEGFSIKDKAYYNVLKGKVCHEYF
MLHFGQLDHEDENIIYENYLQEV
>Mature_502_residues
SIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDTKFRVNLLKKDKVYKYFLVSDANHKLLIANLVTEQQAK
DLSFIEKDIMKIASSATAYGASDIHFIREDRICKIKFRVNGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQD
ANIILVINEKAYRFRYAHMPLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADTDAIARILNTSYGLFIVSGTTGSG
KSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLISGAQQSSIVADNDDKTKNPFADAVRSAMRRDPDVIMIGEIRDKPTV
EALSSAVESGHYCLTTIHAGSVVSVLQRLSGLGMKADKIASPGFLAGITSQKLIPELCPSCKVSFVDERYQRAVFSANEN
GCEACNHSGFKGRLLLLETLVPTVEDLELVASENWVSLYRKYRERRFIKTGKKGLGEGFSIKDKAYYNVLKGKVCHEYFM
LHFGQLDHEDENIIYENYLQEV

Specific function: Involved in the translocation of the tcpA pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=397, Percent_Identity=30.2267002518892, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1786296, Length=437, Percent_Identity=27.6887871853547, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87082188, Length=183, Percent_Identity=32.7868852459016, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57293; Mature: 57162

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDTKFRVNLLKKDKVYKYF
CCCCCEEHHHCCCCHHHHHCCCCHHHHHHCCCHHHHEEHHHCCCCEEEEEEECCCEEEEE
LVSDANHKLLIANLVTEQQAKDLSFIEKDIMKIASSATAYGASDIHFIREDRICKIKFRV
EEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEEE
NGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQDANIILVINEKAYRFRYAHM
CCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEECC
PLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADTDAIARILNTSYGLFIVSGTTGS
CEECCCCCCEEEEEEEEECCCCEEEECCHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCC
GKSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLISGAQQSSIVADNDDKTKNPFADAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCEEECCCCCCCCHHHHHHH
SAMRRDPDVIMIGEIRDKPTVEALSSAVESGHYCLTTIHAGSVVSVLQRLSGLGMKADKI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCHHHC
ASPGFLAGITSQKLIPELCPSCKVSFVDERYQRAVFSANENGCEACNHSGFKGRLLLLET
CCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEHHH
LVPTVEDLELVASENWVSLYRKYRERRFIKTGKKGLGEGFSIKDKAYYNVLKGKVCHEYF
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
MLHFGQLDHEDENIIYENYLQEV
HHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDTKFRVNLLKKDKVYKYF
CCCCEEHHHCCCCHHHHHCCCCHHHHHHCCCHHHHEEHHHCCCCEEEEEEECCCEEEEE
LVSDANHKLLIANLVTEQQAKDLSFIEKDIMKIASSATAYGASDIHFIREDRICKIKFRV
EEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEEE
NGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQDANIILVINEKAYRFRYAHM
CCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEECC
PLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADTDAIARILNTSYGLFIVSGTTGS
CEECCCCCCEEEEEEEEECCCCEEEECCHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCC
GKSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLISGAQQSSIVADNDDKTKNPFADAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCEEECCCCCCCCHHHHHHH
SAMRRDPDVIMIGEIRDKPTVEALSSAVESGHYCLTTIHAGSVVSVLQRLSGLGMKADKI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCHHHC
ASPGFLAGITSQKLIPELCPSCKVSFVDERYQRAVFSANENGCEACNHSGFKGRLLLLET
CCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEHHH
LVPTVEDLELVASENWVSLYRKYRERRFIKTGKKGLGEGFSIKDKAYYNVLKGKVCHEYF
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
MLHFGQLDHEDENIIYENYLQEV
HHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8097178; 10952301 [H]