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The map label for this gene is acnB [H]

Identifier: 147673282

GI number: 147673282

Start: 124676

End: 127273

Strand: Direct

Name: acnB [H]

Synonym: VC0395_A0133

Alternate gene names: 147673282

Gene position: 124676-127273 (Clockwise)

Preceding gene: 147675227

Following gene: 147673206

Centisome position: 4.12

GC content: 51.27

Gene sequence:

>2598_bases
GTGCTTGAAGCCTACCGTAAACACGTCGCAGAGCGTGCCGCTGAAGGAGTTGTACCTAAACCCCTAGACGCAGAGCAAGT
CGCAGGACTTGTCGAACTTCTGAAAAATCCCCCACAAGGTGAAGAAGCTTTCATTCTTGACCTACTTGAAAATCGCATTC
CTCCGGGTGTCGATGAAGCGGCTTACGTGAAAGCAGGTTTCTTGACGGCCGTTGCGAGAGGCGAGGTCGCATCTCCGTTG
GTGAGTCGTGAAAAAGCGGCTGAGCTACTCGGTACCATGCAAGGCGGCTACAACATCGAACCGCTGATTGAATTACTGGA
CGATAGCGCGCTGGCTCCGATTGCCGCGAAAGCGCTGTCTCATACCCTCTTGATGTTTGATAACTTCTACGATGTGGAAG
AAAAAGCCAAAGCGGGCAACCCATTTGCGAAACAAGTGCTGCAATCTTGGGCTGATGCAGAGTGGTTCCTATCTAAGCCG
GCACTGCCTGAAAAAGTGACCTTAACCGTATTTAAAGTGACGGGGGAGACCAACACGGACGATCTCTCTCCAGCGCCTGA
TGCATGGTCACGTCCTGATATTCCTGTACACGCATTAGCCATGCTGAAAAACGCGCGCGATGGCATTGAGCCGGATGTAC
CGGGTAAAGTTGGCCCGATCAAACAGATCGAAGCACTTAAAGCCAAAGGTCACCAACTGGTTTACGTGGGGGATGTGGTC
GGTACCGGTTCATCACGTAAATCGGCAACCAACTCAGTGTTGTGGTTTATGGGAGACGATATTCCTAATGTGCCTAATAA
GCGCACGGGCGGATATGTGCTTGGCGGTAAAATCGCTCCAATCTTCTTTAACACCATGGAAGATGCTGGCGCGCTGCCGA
TCGAAGTGGATGTCTCTAAGCTCAATATGGGCGATGTGATCGACGTTTACCCATTTGAAGGCAAAGTGTGTAATCACGCG
ACGGGCGAAGTGCTTGCGACTTTCAAACTGAAAACCGATGTCCTGTACGATGAAGTGCGTGCGGGTGGTCGTATTCCGCT
GATCGTTGGACGTGGCCTGACGGATAAAGCGCGTCACGCATTAGGTCTGGAAGCGTCAAAAGAGTTTCGTCGCCCTGTGG
CGGTTGCAGATAGCGGTAAAGGTTATACGCTGGCTCAGAAAATGGTGGGTAAAGCGTGTGGTGTACAAGGCATTCGCCCA
GGCACTTACTGTGAACCTAAGATGACGACTGTCGGTTCGCAAGATACCACTGGCCCAATGACGCGTGATGAATTGAAAGA
TTTGGCCTGTCTTGGTTTCTCTGCCGATTTAGTGATGCAGTCTTTCTGTCATACCTCGGCTTATCCAAAGCCTGTCGATG
TGCAGACTCACCACACATTACCTGATTTCATCATGAACCGTGGCGGTGTTTCACTGCGTCCGGGCGATGGTGTTATCCAC
TCATGGTTAAACCGCATGCTACTGCCAGATACAGTCGGTACTGGTGGTGACTCGCATACTCGTTTCCCATTGGGTATTTC
TTTCCCAGCAGGTTCTGGTCTGGTGGCGTTTGCTGCCGCGACTGGGGTAATGCCACTGGATATGCCTGAATCGGTTCTGG
TGCGCTTTAAGGGTAAGATGCAACCGGGGATCACGTTGCGTGATCTGGTGCATGCCATCCCTTATTACGGCATCAAGCAA
GGTCTGCTCACAGTAGAAAAAGCAGGTAAGATCAACGAATTCTCCGGTCGTATCCTAGAGATCGAAGGGGTTGAACACCT
GACGGTTGAGCAGGCGTTTGAGCTTTCTGATGCATCCGCAGAACGTTCTGCGGCGGGTTGTACGGTGAAACTGTCACAAG
CATCGATTGAAGAGTACCTCAACTCGAATATCGTGATGTTGAAGTGGATGATTGCTGAAGGTTATGGCGATCGCCGTACT
ATTGAGCGCCGTATTCAAGCGATGGAAGCTTGGTTAGCTAACCCAACACTGATGGAAGCGGACAAAGATGCCCAGTACGC
GCATGTGATTGAGATTGATCTTGCTGAGATCAAAGAGCCAATCCTATGTGCGCCAAACGATCCAGACGATGCTCGTCTGC
TTTCTGAGTGTGCGGGTGAAACCATCAATGAAGTGTTCATCGGCTCTTGTATGACCAACATCGGGCACTTCCGCGCGGCG
GGTAAATTGCTTGAAAAATTCAATGGCCAATTGAATACCCGTTTGTGGGTCGCACCGCCAACCAAGATGGATCGTGACCA
GCTGATTGAAGAGGGTTACTACGGTATTTTCGGTCGTGCTGGCGTGCGTATCGAAACGCCGGGATGTTCGTTGTGTATGG
GTAACCAAGCACGCGTAGCGGACAAAGCAACCGTGATGTCGACTTCAACGCGTAACTTCCCGAACCGTTTGGGCACAGGG
GCTAACGTTTACCTCTCTTCTGCTGAATTAGCGGCAGTGGGAGCGATTTTGGGTCGAATCCCAAGCGTAGCGGAGTACAT
GGATTACGCGAAGCAGATTGATGCGGCGGCCGCGGATACCTACCGTTACCTCAACTTCCACCGTATGGGTCAGTACACCA
GTAAAGCAGATTCGGTCATTTTCCAAGAACCTGCATAA

Upstream 100 bases:

>100_bases
AATTTGAGTGAAAACAAGGGCTAGGGAGAGCCAAGTTTCCAAGGATGTTCGCTCGTATTTTTATAATCAACTGGGTGAAC
CGCAATGAGAGGAAAAAGTC

Downstream 100 bases:

>100_bases
GCAGAGTGATTGAATCCTCTTTTAAAGCCGCGCTTGTCGCGGCTTTATTGTGGGTATTTTTCGGTCGGTTTACTTCGCGG
GTCATCACTTGTCTAAGTAT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRKHVAERAAEGVVPKPLDAEQVAGLVELLKNPPQGEEAFILDLLENRIPPGVDEAAYVKAGFLTAVARGEVASPL
VSREKAAELLGTMQGGYNIEPLIELLDDSALAPIAAKALSHTLLMFDNFYDVEEKAKAGNPFAKQVLQSWADAEWFLSKP
ALPEKVTLTVFKVTGETNTDDLSPAPDAWSRPDIPVHALAMLKNARDGIEPDVPGKVGPIKQIEALKAKGHQLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRTGGYVLGGKIAPIFFNTMEDAGALPIEVDVSKLNMGDVIDVYPFEGKVCNHA
TGEVLATFKLKTDVLYDEVRAGGRIPLIVGRGLTDKARHALGLEASKEFRRPVAVADSGKGYTLAQKMVGKACGVQGIRP
GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPYYGIKQ
GLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASAERSAAGCTVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRT
IERRIQAMEAWLANPTLMEADKDAQYAHVIEIDLAEIKEPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAA
GKLLEKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGTG
ANVYLSSAELAAVGAILGRIPSVAEYMDYAKQIDAAAADTYRYLNFHRMGQYTSKADSVIFQEPA

Sequences:

>Translated_865_residues
MLEAYRKHVAERAAEGVVPKPLDAEQVAGLVELLKNPPQGEEAFILDLLENRIPPGVDEAAYVKAGFLTAVARGEVASPL
VSREKAAELLGTMQGGYNIEPLIELLDDSALAPIAAKALSHTLLMFDNFYDVEEKAKAGNPFAKQVLQSWADAEWFLSKP
ALPEKVTLTVFKVTGETNTDDLSPAPDAWSRPDIPVHALAMLKNARDGIEPDVPGKVGPIKQIEALKAKGHQLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRTGGYVLGGKIAPIFFNTMEDAGALPIEVDVSKLNMGDVIDVYPFEGKVCNHA
TGEVLATFKLKTDVLYDEVRAGGRIPLIVGRGLTDKARHALGLEASKEFRRPVAVADSGKGYTLAQKMVGKACGVQGIRP
GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPYYGIKQ
GLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASAERSAAGCTVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRT
IERRIQAMEAWLANPTLMEADKDAQYAHVIEIDLAEIKEPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAA
GKLLEKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGTG
ANVYLSSAELAAVGAILGRIPSVAEYMDYAKQIDAAAADTYRYLNFHRMGQYTSKADSVIFQEPA
>Mature_865_residues
MLEAYRKHVAERAAEGVVPKPLDAEQVAGLVELLKNPPQGEEAFILDLLENRIPPGVDEAAYVKAGFLTAVARGEVASPL
VSREKAAELLGTMQGGYNIEPLIELLDDSALAPIAAKALSHTLLMFDNFYDVEEKAKAGNPFAKQVLQSWADAEWFLSKP
ALPEKVTLTVFKVTGETNTDDLSPAPDAWSRPDIPVHALAMLKNARDGIEPDVPGKVGPIKQIEALKAKGHQLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRTGGYVLGGKIAPIFFNTMEDAGALPIEVDVSKLNMGDVIDVYPFEGKVCNHA
TGEVLATFKLKTDVLYDEVRAGGRIPLIVGRGLTDKARHALGLEASKEFRRPVAVADSGKGYTLAQKMVGKACGVQGIRP
GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPYYGIKQ
GLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASAERSAAGCTVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRT
IERRIQAMEAWLANPTLMEADKDAQYAHVIEIDLAEIKEPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAA
GKLLEKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGTG
ANVYLSSAELAAVGAILGRIPSVAEYMDYAKQIDAAAADTYRYLNFHRMGQYTSKADSVIFQEPA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=82.3665893271462, Blast_Score=1445, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322261, Length=406, Percent_Identity=26.6009852216749, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323335, Length=380, Percent_Identity=26.0526315789474, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93588; Mature: 93588

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVAERAAEGVVPKPLDAEQVAGLVELLKNPPQGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAVARGEVASPLVSREKAAELLGTMQGGYNIEPLIELLDDSALAPIAAKALS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHH
HTLLMFDNFYDVEEKAKAGNPFAKQVLQSWADAEWFLSKPALPEKVTLTVFKVTGETNTD
HHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPVHALAMLKNARDGIEPDVPGKVGPIKQIEALKAKGHQLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRTGGYVLGGKIAPIFFNTMEDAGALPIEVDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
LNMGDVIDVYPFEGKVCNHATGEVLATFKLKTDVLYDEVRAGGRIPLIVGRGLTDKARHA
CCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLEASKEFRRPVAVADSGKGYTLAQKMVGKACGVQGIRPGTYCEPKMTTVGSQDTTGPM
HCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCC
TRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPYYGIKQGLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASA
CCCCCHHHHHHHHCCHHHHHCCEEHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCH
ERSAAGCTVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRTIERRIQAMEAWLANPTLMEA
HHCCCCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEC
DKDAQYAHVIEIDLAEIKEPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAA
CCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLLEKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVA
HHHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCEEECCCCCCC
DKATVMSTSTRNFPNRLGTGANVYLSSAELAAVGAILGRIPSVAEYMDYAKQIDAAAADT
CHHHEEECCCCCCHHHHCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YRYLNFHRMGQYTSKADSVIFQEPA
HHHHHHHHHCCHHCCCCCEEEECCC
>Mature Secondary Structure
MLEAYRKHVAERAAEGVVPKPLDAEQVAGLVELLKNPPQGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAVARGEVASPLVSREKAAELLGTMQGGYNIEPLIELLDDSALAPIAAKALS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHH
HTLLMFDNFYDVEEKAKAGNPFAKQVLQSWADAEWFLSKPALPEKVTLTVFKVTGETNTD
HHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPVHALAMLKNARDGIEPDVPGKVGPIKQIEALKAKGHQLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRTGGYVLGGKIAPIFFNTMEDAGALPIEVDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
LNMGDVIDVYPFEGKVCNHATGEVLATFKLKTDVLYDEVRAGGRIPLIVGRGLTDKARHA
CCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLEASKEFRRPVAVADSGKGYTLAQKMVGKACGVQGIRPGTYCEPKMTTVGSQDTTGPM
HCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCC
TRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPYYGIKQGLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASA
CCCCCHHHHHHHHCCHHHHHCCEEHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCH
ERSAAGCTVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRTIERRIQAMEAWLANPTLMEA
HHCCCCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEC
DKDAQYAHVIEIDLAEIKEPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAA
CCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLLEKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVA
HHHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCEEECCCCCCC
DKATVMSTSTRNFPNRLGTGANVYLSSAELAAVGAILGRIPSVAEYMDYAKQIDAAAADT
CHHHEEECCCCCCHHHHCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YRYLNFHRMGQYTSKADSVIFQEPA
HHHHHHHHHCCHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]