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The map label for this gene is hlyB [H]

Identifier: 147673263

GI number: 147673263

Start: 2169619

End: 2170920

Strand: Reverse

Name: hlyB [H]

Synonym: VC0395_A2016

Alternate gene names: 147673263

Gene position: 2170920-2169619 (Counterclockwise)

Preceding gene: 147675584

Following gene: 147673756

Centisome position: 71.79

GC content: 49.77

Gene sequence:

>1302_bases
TTGCATCCTCACTCAGTGGCAATCAAAAAGAATGGAGAACTGACAATGGCAACGATGTCGTTTAAGCCGTGGGAGCGGGT
AATTACAGATGTTCGTCTCGTCCCGAAAATGGTGATGCTGATGGTATTCAGCACGGTGTTGATCATTGGCAAGCAGCTTT
GGGACGCAGATATCTTTTACCAATCTTTATTGGCGGCTACCAACAGTGTGGAAGTCGCACAGCAGCACTACGATAACTAT
CTGGTGCAAGTGTTCTGGCAAACCTTGTTACTGATTGTGGTGTTTGTGGTGCTGTTGCTGTTTGCCGCGCGCGTCATGCT
GCGACAAACTCATTATCTGAGTGATGTGATCAAGCTGATGGCGCAGCGTAACCTCTCAGAGCCGATCAAAATGGATTGTA
AAGATGAGTATGGGGATGTCGCGCGCGAGTTGGAGAAAACCCGCCGCCAGCTACAAGACCTGATCAAGTTGCAAATTGAT
GCCTCGCAAGAGCTGACCTCGCTCACTGAAGTGATGACCATGAGCATGTCGGAGACCAAAGATTCAGCGCAAGAAGAGTT
CAACGAAATTGACCAATTGGCCAAAGCGATGAGTGAAATGTCGTCAACGGTTCAAACGGTCGCGGAACATGCGCAGAACG
CCTCTTCCTTAACCGAAAATGCGTCTCAGCAGGCTCAAACCGGTCAGCAGTTTGTCAAAAGCACCATCAGTAAAATGAGT
GAGCTTTCGAAAGACATCAGCTCTTCTGCGCAAGCCGTCAATCAGGTGGAAGAGCGAGTGGTGGCGATCAGCAGCGTGGT
GGGCACGATTCGCAGCATTTCAGAGCAAACCAATTTGCTGGCACTGAACGCGGCGATTGAAGCGGCGCGAGCAGGAGAAG
CCGGGCGCGGTTTTGCTGTGGTTGCCGATGAAGTACGAAATTTGGCACAGCGTACCCAAAATGCGACCGTTGAAATCCAA
GAGATGATTTCACAGCTGCAAACTAGTGCCAATAGCGCTGTGAATTTAATGGAAAAAAGTGTGGTGCAAGCGGCGGATGG
GGTAGAGCTCATCACCAACGCTGGTAGCGAGTTGGATGGCATCGTTAATCAGGTTCAATTGCTCAATGACATGAACTTCC
AAATTGCCGCGGCAGCAGGCCAGCAAAGCAATGTGGCCGAAGAGATGAACGAAAACCTCAACAATGTGCGTGAGTTGGTG
GAAGGTTCGGTCACTGTGATGACTGAGCTGCTCGAAACTTCAGAAATCATGCAAAGTAACGCGGAAGAGCTGGATAGAAA
AATTACCGCATTTCGCGTGTAG

Upstream 100 bases:

>100_bases
GATGAATAAGAGAATTAGCGATAACAAACTGATGGGTTTTGTTTCATTTTTGATATTATCGGCTTTCATACGAGGCTCGA
AATAATAGGTTGCGGATCTT

Downstream 100 bases:

>100_bases
GCTGAGCTATTCAGCAAAACCGCAAAAAATTATTCACCAACCGCCCACAACTGCTCTGTGGGCGTTTTTTTTCCGCCAGA
GTTTGCGATATAACTAGTGA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MHPHSVAIKKNGELTMATMSFKPWERVITDVRLVPKMVMLMVFSTVLIIGKQLWDADIFYQSLLAATNSVEVAQQHYDNY
LVQVFWQTLLLIVVFVVLLLFAARVMLRQTHYLSDVIKLMAQRNLSEPIKMDCKDEYGDVARELEKTRRQLQDLIKLQID
ASQELTSLTEVMTMSMSETKDSAQEEFNEIDQLAKAMSEMSSTVQTVAEHAQNASSLTENASQQAQTGQQFVKSTISKMS
ELSKDISSSAQAVNQVEERVVAISSVVGTIRSISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQNATVEIQ
EMISQLQTSANSAVNLMEKSVVQAADGVELITNAGSELDGIVNQVQLLNDMNFQIAAAAGQQSNVAEEMNENLNNVRELV
EGSVTVMTELLETSEIMQSNAEELDRKITAFRV

Sequences:

>Translated_433_residues
MHPHSVAIKKNGELTMATMSFKPWERVITDVRLVPKMVMLMVFSTVLIIGKQLWDADIFYQSLLAATNSVEVAQQHYDNY
LVQVFWQTLLLIVVFVVLLLFAARVMLRQTHYLSDVIKLMAQRNLSEPIKMDCKDEYGDVARELEKTRRQLQDLIKLQID
ASQELTSLTEVMTMSMSETKDSAQEEFNEIDQLAKAMSEMSSTVQTVAEHAQNASSLTENASQQAQTGQQFVKSTISKMS
ELSKDISSSAQAVNQVEERVVAISSVVGTIRSISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQNATVEIQ
EMISQLQTSANSAVNLMEKSVVQAADGVELITNAGSELDGIVNQVQLLNDMNFQIAAAAGQQSNVAEEMNENLNNVRELV
EGSVTVMTELLETSEIMQSNAEELDRKITAFRV
>Mature_433_residues
MHPHSVAIKKNGELTMATMSFKPWERVITDVRLVPKMVMLMVFSTVLIIGKQLWDADIFYQSLLAATNSVEVAQQHYDNY
LVQVFWQTLLLIVVFVVLLLFAARVMLRQTHYLSDVIKLMAQRNLSEPIKMDCKDEYGDVARELEKTRRQLQDLIKLQID
ASQELTSLTEVMTMSMSETKDSAQEEFNEIDQLAKAMSEMSSTVQTVAEHAQNASSLTENASQQAQTGQQFVKSTISKMS
ELSKDISSSAQAVNQVEERVVAISSVVGTIRSISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQNATVEIQ
EMISQLQTSANSAVNLMEKSVVQAADGVELITNAGSELDGIVNQVQLLNDMNFQIAAAAGQQSNVAEEMNENLNNVRELV
EGSVTVMTELLETSEIMQSNAEELDRKITAFRV

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=306, Percent_Identity=31.6993464052288, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788195, Length=267, Percent_Identity=32.9588014981273, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788194, Length=365, Percent_Identity=27.9452054794521, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787690, Length=355, Percent_Identity=27.6056338028169, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789453, Length=371, Percent_Identity=25.8760107816712, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 47917; Mature: 47917

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPHSVAIKKNGELTMATMSFKPWERVITDVRLVPKMVMLMVFSTVLIIGKQLWDADIFY
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLLAATNSVEVAQQHYDNYLVQVFWQTLLLIVVFVVLLLFAARVMLRQTHYLSDVIKLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRNLSEPIKMDCKDEYGDVARELEKTRRQLQDLIKLQIDASQELTSLTEVMTMSMSETK
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DSAQEEFNEIDQLAKAMSEMSSTVQTVAEHAQNASSLTENASQQAQTGQQFVKSTISKMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSKDISSSAQAVNQVEERVVAISSVVGTIRSISEQTNLLALNAAIEAARAGEAGRGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VADEVRNLAQRTQNATVEIQEMISQLQTSANSAVNLMEKSVVQAADGVELITNAGSELDG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHH
IVNQVQLLNDMNFQIAAAAGQQSNVAEEMNENLNNVRELVEGSVTVMTELLETSEIMQSN
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
AEELDRKITAFRV
HHHHHHHHHHHCC
>Mature Secondary Structure
MHPHSVAIKKNGELTMATMSFKPWERVITDVRLVPKMVMLMVFSTVLIIGKQLWDADIFY
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLLAATNSVEVAQQHYDNYLVQVFWQTLLLIVVFVVLLLFAARVMLRQTHYLSDVIKLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRNLSEPIKMDCKDEYGDVARELEKTRRQLQDLIKLQIDASQELTSLTEVMTMSMSETK
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DSAQEEFNEIDQLAKAMSEMSSTVQTVAEHAQNASSLTENASQQAQTGQQFVKSTISKMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSKDISSSAQAVNQVEERVVAISSVVGTIRSISEQTNLLALNAAIEAARAGEAGRGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VADEVRNLAQRTQNATVEIQEMISQLQTSANSAVNLMEKSVVQAADGVELITNAGSELDG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHH
IVNQVQLLNDMNFQIAAAAGQQSNVAEEMNENLNNVRELVEGSVTVMTELLETSEIMQSN
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
AEELDRKITAFRV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]