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The map label for this gene is pmbA [H]

Identifier: 147673242

GI number: 147673242

Start: 2270057

End: 2271400

Strand: Reverse

Name: pmbA [H]

Synonym: VC0395_A2115

Alternate gene names: 147673242

Gene position: 2271400-2270057 (Counterclockwise)

Preceding gene: 147674110

Following gene: 147674625

Centisome position: 75.11

GC content: 50.3

Gene sequence:

>1344_bases
ATGGATGTAAAAGAGCGAGTTGCGCTGCAGCGTCAAGAGCTGGAAGCTGCGGTTGCTAAGGCATTAGAGCTTGCAGCGGC
GAGTTCGGATGCGGCTGAAGTGGCGATCACCAAGAGCACGGGATTAAGTGTTTCGACCCGCATGTGTGAAGTTGAAAATG
TTGAATTCAACAGTGATGGTGCACTTGGAATTACTGTTTACCGCGGTCAACGTAAAGGCAGCGCTTCAACATCGGACTTG
AGTGAAAAAGCGATTCGACAAACCGTATTAGCCGCGCTGGATATTGCTCAGTATACTTCGGCCGATCCTTATGCTGGCCC
TGCGCCGAAAGAATTGATGGTGCAGGATATTCCTGATCTTGACCTCTTCCATCCTGATGAGCCGAATCCCGATGTGGCGG
CGCAAATTGCCATTGCGGCAGAACGTGCGGCCTTAGGCTACAGCAAGAAAATTAAGCAAAGCGATGGCGGTAGTTATGAT
AGCCATTTCGGCATTCGTGTTTACGGCAACAGCCACGGTGTATTGGCTAGCTATGCCTCTAGTCGCCACAGCATTAGCTG
TAGTGTGATTGGTGAAGGCCGCAACGGTGAAATGGAGCGTGATTACAGCTACACCGTGGCGCGTCACCGTGACGATTTAT
GGACGCCAGAAAGCGTGGGTTTGCAAGCGGCGGAAAATACGGTGAGCCGTCTGGATCCGCAAAAGCTGAAAACGGGTCAG
TTTCCAGTGATGTTTGCGGCAGATGTAGCGACAGGTTTAATCGGTCATCTGGTGATGGCGATCAGCGGTGGTAACTTATA
TCGCAAATCGAGTTTCTTACTCGATCATCTTGGCAAAACCATTCTGCCGACGTGGTTCAATATCAGTGAGCGCCCGCATG
TGATGCGTGGTTTGGCTTCCAGCCCATTTGATAGTGAAGGGGTTCGTACCCAAGATCTGGAAATCATCACTGATGGTGTG
TTGGCAACTTACTTACTGACCAGTTACGCCGCGCGCAAAATGAATATGACGCCAACCGGTCATGCGGGTGGCATTCATAA
CTGGTATGTGAAATCGACTGGTCAAAACTACCAACAGATGCTCAAAGAGCTGGGTACCGGCTTATTGGTGACCGAAGTGA
TGGGACAAGGTGTGAATGTGGTGACGGGAGATTACTCACGTGGCGCTGCTGGTTTCTGGGTAGAAAACGGTGAAATTAAA
TACCCTGTTTCTGAAATCACCATTGCGGGTAACCTCAAGCAGATGTTGCAGCAGATTGTCGCGGTGGGCAGTGATATCGA
AACGCGCTCCCAGATCCAAACCGGTTCTATTTTGATTGAATCAATGAAGGTCGCAGGCGAGTAA

Upstream 100 bases:

>100_bases
ATGATGAGTTACTTATGTCGGCTATTTTAGCAAGAATCGTGGGGAGAAAGCGAAATTCTTGTTATCCTAATAACATCAAT
AACCAATGGTTGAATGAAAT

Downstream 100 bases:

>100_bases
GCTCTGAGCCATTGAATGAAAAAGCCGACAGAAAGTCGGCTTTTTTGTCGAATCAATTTGGGAAATCGGACATTAACTGG
CCAGCAGCAAAGTGGCTAGA

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQDIPDLDLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYD
SHFGIRVYGNSHGVLASYASSRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLASSPFDSEGVRTQDLEIITDGV
LATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIK
YPVSEITIAGNLKQMLQQIVAVGSDIETRSQIQTGSILIESMKVAGE

Sequences:

>Translated_447_residues
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQDIPDLDLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYD
SHFGIRVYGNSHGVLASYASSRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLASSPFDSEGVRTQDLEIITDGV
LATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIK
YPVSEITIAGNLKQMLQQIVAVGSDIETRSQIQTGSILIESMKVAGE
>Mature_447_residues
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQDIPDLDLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYD
SHFGIRVYGNSHGVLASYASSRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLASSPFDSEGVRTQDLEIITDGV
LATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIK
YPVSEITIAGNLKQMLQQIVAVGSDIETRSQIQTGSILIESMKVAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=63.9821029082774, Blast_Score=592, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48141; Mature: 48141

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEECEEECCCC
ALGITVYRGQRKGSASTSDLSEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQDIPDL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
DLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYDSHFGIRVYGNSHGVLASYAS
CCCCCCCCCCCHHHEEHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCCEEEHHCC
SRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
CCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCHHHHCCCC
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLAS
CCEEEHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHCCHHCCCCCCCHHHHHCCC
SPFDSEGVRTQDLEIITDGVLATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQM
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
LKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIKYPVSEITIAGNLKQMLQQIV
HHHHCCCEEEHHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHEEECCCHHHHHHHHH
AVGSDIETRSQIQTGSILIESMKVAGE
HCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVKERVALQRQELEAAVAKALELAAASSDAAEVAITKSTGLSVSTRMCEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEECEEECCCC
ALGITVYRGQRKGSASTSDLSEKAIRQTVLAALDIAQYTSADPYAGPAPKELMVQDIPDL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
DLFHPDEPNPDVAAQIAIAAERAALGYSKKIKQSDGGSYDSHFGIRVYGNSHGVLASYAS
CCCCCCCCCCCHHHEEHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCCEEEHHCC
SRHSISCSVIGEGRNGEMERDYSYTVARHRDDLWTPESVGLQAAENTVSRLDPQKLKTGQ
CCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCHHHHCCCC
FPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKTILPTWFNISERPHVMRGLAS
CCEEEHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHCCHHCCCCCCCHHHHHCCC
SPFDSEGVRTQDLEIITDGVLATYLLTSYAARKMNMTPTGHAGGIHNWYVKSTGQNYQQM
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
LKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGEIKYPVSEITIAGNLKQMLQQIV
HHHHCCCEEEHHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHEEECCCHHHHHHHHH
AVGSDIETRSQIQTGSILIESMKVAGE
HCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]