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The map label for this gene is thiI [H]

Identifier: 147673207

GI number: 147673207

Start: 443586

End: 445133

Strand: Direct

Name: thiI [H]

Synonym: VC0395_A0417

Alternate gene names: 147673207

Gene position: 443586-445133 (Clockwise)

Preceding gene: 147674334

Following gene: 147675583

Centisome position: 14.67

GC content: 48.0

Gene sequence:

>1548_bases
GTGATCGTGTATAATCGCGCCCCTTTAGCGGTAAGCGCCTTGCCAAGGTTTACCTGCTGCTCGCCAGATTGTCTAACTCA
GATTGCGAATAACTGTATGAAATTTATCGTTAAACCCCATCCGGAAATTTTTGTGAAAAGTGAATCGGTACGTAAGCGTT
TCACAAAGATCCTTGAGAGCAATATTCGAATTATTGTGAAAGCCCGCACACAAGGGGTGGCGGTATTCAATCGTCGTGAT
CATATTGAAGTGACGTCAAACAGCGATACTTATTACGCCGAAGTGTTGGAGATTCTGACGACCACACCGGGTATCCAGCA
AGTGTTGGAAGTGCAGCAATCAAGCTTTACCGATCTGCACAACATCTACGAGCAAGTGCTGGAGCTAAATCGCGCTAACC
TCGAAAACAAAACCTTTGTTGTGCGCGCGAAACGCCGTGGTAAGCATGATTTTACCTCTATTGAACTCGAACGTTATGTT
GGGGGTGGCCTCAATCAAGCCATCGCCAGTGCCAAGGTAAAATTGATTAACCCTGACGTGACCGTGCAAGTGGAAGTGGT
CGATGAGCTGCTTAACCAAGTGATCGCGCGTCATAAAGGTTTAGGTGGTTTCCCTCTGGGGACCCAAGAAGATGTATTGA
GCCTGATTTCTGGTGGCTTCGACTCCGGTGTGTCGAGCTATCTGCACATTAAACGTGGTTCAAAAGTGCATTACTGCTTC
TTTAATCTGGGGGGACCCGCCCACGAAATTGGTGTGAAGCAAACCGCTTACTACCTGTGGCAAAAATACGGTTCATCGGC
CAAAGTGCGATTTATCGCGATCGATTTTGCTCCTGTGGTGGCTGAGATCCTCGAGAAGATCGATGATGGTCAAATGGGCG
TGGTGCTCAAGCGTATGTTTATGCGCACCGCCGGTATGGTGGCTGAGAAGTTTGGCATTCAAGCTTTGGTTACGGGTGAA
GCGCTAGGCCAGGTTTCTAGCCAAACCCTGACTAACCTGCGCCATATCGATAACGTGACCGATACTTTGATTCTGCGTCC
GCTCATCAACTGGGATAAAGAAGACATCATCCGTCTGGCGCGTGAAATTGGTACGGAAGATTTCGCCAAAACCATGCCTG
AATTCTGCGGGGTGATTTCAAAAAGCCCAACCGTAAAAGCGGTAAAAGAGAAATTGGAAGAAGAAGAAGCCAAATTCGAT
TTTGCTCTGCTTGATCAAGTGGTGTACAACGCGCGCCAAATCGACATTCGTGATATTGGTAAAGAGTCGCTGGAAAAAGC
CCCTGAAGTGGAGTTGGTTAACAGCGCAGAAGAGGGTAACGCCGTCGTACTGGATATTCGTAGCCCAGACGAAGAGGACG
AAAGCCCGCTAGAGATTGCGGGTGTGGAAGTGAAGCATCTGCCTTTCTATAAGCTTGCGACTCAATTTTGTGACCTCGAT
CAGTCAAAAACCTACTTGCTGTACTGCTCACGTGGCGTGATGAGCCGCTTACAAGCGCTGTACCTGCAAGAACAAGGGTT
TAACAATGTGAAAGTTTATCGTCCATAG

Upstream 100 bases:

>100_bases
ACCTGCAACTCCAAGTAGTTTGGGTATATACACACCGACTGTAAACTAAAAAAGGCAAGCCATTGGGCTTGTCTTTTTTA
TGGGCTTTTCTCAATAAAGT

Downstream 100 bases:

>100_bases
TTCATGGTCGATAGTTCATTGCCCATAACGCGATCATGAATGGATAAAACGCAGAGTTTGCTCAGCAGGCTCTGCGTTTT
TTTCGCTTTATTCCCTTCCT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MIVYNRAPLAVSALPRFTCCSPDCLTQIANNCMKFIVKPHPEIFVKSESVRKRFTKILESNIRIIVKARTQGVAVFNRRD
HIEVTSNSDTYYAEVLEILTTTPGIQQVLEVQQSSFTDLHNIYEQVLELNRANLENKTFVVRAKRRGKHDFTSIELERYV
GGGLNQAIASAKVKLINPDVTVQVEVVDELLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCF
FNLGGPAHEIGVKQTAYYLWQKYGSSAKVRFIAIDFAPVVAEILEKIDDGQMGVVLKRMFMRTAGMVAEKFGIQALVTGE
ALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIRLAREIGTEDFAKTMPEFCGVISKSPTVKAVKEKLEEEEAKFD
FALLDQVVYNARQIDIRDIGKESLEKAPEVELVNSAEEGNAVVLDIRSPDEEDESPLEIAGVEVKHLPFYKLATQFCDLD
QSKTYLLYCSRGVMSRLQALYLQEQGFNNVKVYRP

Sequences:

>Translated_515_residues
MIVYNRAPLAVSALPRFTCCSPDCLTQIANNCMKFIVKPHPEIFVKSESVRKRFTKILESNIRIIVKARTQGVAVFNRRD
HIEVTSNSDTYYAEVLEILTTTPGIQQVLEVQQSSFTDLHNIYEQVLELNRANLENKTFVVRAKRRGKHDFTSIELERYV
GGGLNQAIASAKVKLINPDVTVQVEVVDELLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCF
FNLGGPAHEIGVKQTAYYLWQKYGSSAKVRFIAIDFAPVVAEILEKIDDGQMGVVLKRMFMRTAGMVAEKFGIQALVTGE
ALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIRLAREIGTEDFAKTMPEFCGVISKSPTVKAVKEKLEEEEAKFD
FALLDQVVYNARQIDIRDIGKESLEKAPEVELVNSAEEGNAVVLDIRSPDEEDESPLEIAGVEVKHLPFYKLATQFCDLD
QSKTYLLYCSRGVMSRLQALYLQEQGFNNVKVYRP
>Mature_515_residues
MIVYNRAPLAVSALPRFTCCSPDCLTQIANNCMKFIVKPHPEIFVKSESVRKRFTKILESNIRIIVKARTQGVAVFNRRD
HIEVTSNSDTYYAEVLEILTTTPGIQQVLEVQQSSFTDLHNIYEQVLELNRANLENKTFVVRAKRRGKHDFTSIELERYV
GGGLNQAIASAKVKLINPDVTVQVEVVDELLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCF
FNLGGPAHEIGVKQTAYYLWQKYGSSAKVRFIAIDFAPVVAEILEKIDDGQMGVVLKRMFMRTAGMVAEKFGIQALVTGE
ALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIRLAREIGTEDFAKTMPEFCGVISKSPTVKAVKEKLEEEEAKFD
FALLDQVVYNARQIDIRDIGKESLEKAPEVELVNSAEEGNAVVLDIRSPDEEDESPLEIAGVEVKHLPFYKLATQFCDLD
QSKTYLLYCSRGVMSRLQALYLQEQGFNNVKVYRP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=483, Percent_Identity=65.2173913043478, Blast_Score=640, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF00581 Rhodanese; PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 57815; Mature: 57815

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVYNRAPLAVSALPRFTCCSPDCLTQIANNCMKFIVKPHPEIFVKSESVRKRFTKILES
CEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHC
NIRIIVKARTQGVAVFNRRDHIEVTSNSDTYYAEVLEILTTTPGIQQVLEVQQSSFTDLH
CCEEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NIYEQVLELNRANLENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAIASAKVKLINPDV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHEEHHHHHCCCHHHHHHHCEEEEECCCC
TVQVEVVDELLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCF
EEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCEEEEEE
FNLGGPAHEIGVKQTAYYLWQKYGSSAKVRFIAIDFAPVVAEILEKIDDGQMGVVLKRMF
EECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
MRTAGMVAEKFGIQALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIRLA
HHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
REIGTEDFAKTMPEFCGVISKSPTVKAVKEKLEEEEAKFDFALLDQVVYNARQIDIRDIG
HHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHCC
KESLEKAPEVELVNSAEEGNAVVLDIRSPDEEDESPLEIAGVEVKHLPFYKLATQFCDLD
HHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCEEECEEEECCCHHHHHHHHHCCC
QSKTYLLYCSRGVMSRLQALYLQEQGFNNVKVYRP
CCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MIVYNRAPLAVSALPRFTCCSPDCLTQIANNCMKFIVKPHPEIFVKSESVRKRFTKILES
CEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHC
NIRIIVKARTQGVAVFNRRDHIEVTSNSDTYYAEVLEILTTTPGIQQVLEVQQSSFTDLH
CCEEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NIYEQVLELNRANLENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAIASAKVKLINPDV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHEEHHHHHCCCHHHHHHHCEEEEECCCC
TVQVEVVDELLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCF
EEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCEEEEEE
FNLGGPAHEIGVKQTAYYLWQKYGSSAKVRFIAIDFAPVVAEILEKIDDGQMGVVLKRMF
EECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
MRTAGMVAEKFGIQALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIRLA
HHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
REIGTEDFAKTMPEFCGVISKSPTVKAVKEKLEEEEAKFDFALLDQVVYNARQIDIRDIG
HHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHCC
KESLEKAPEVELVNSAEEGNAVVLDIRSPDEEDESPLEIAGVEVKHLPFYKLATQFCDLD
HHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCEEECEEEECCCHHHHHHHHHCCC
QSKTYLLYCSRGVMSRLQALYLQEQGFNNVKVYRP
CCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]