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The map label for this gene is ilvD [H]

Identifier: 146343741

GI number: 146343741

Start: 7284172

End: 7285908

Strand: Reverse

Name: ilvD [H]

Synonym: BRADO6988

Alternate gene names: 146343741

Gene position: 7285908-7284172 (Counterclockwise)

Preceding gene: 146343742

Following gene: 146343740

Centisome position: 97.71

GC content: 66.38

Gene sequence:

>1737_bases
ATGAGCAACAAGAAGACCCCCGACCAGCTGCGCAGCGCGCGCTGGTTCGCGCCCGACGATCTCCGCTCGTTCGGCCACCG
TTCGCGGGTGATGCAGATGGGCTATGCCCAGGAGGAGTGGAAGGGCCGCCCGGTGATCGCCATCGTCAACACCTGGTCGG
ACGCACAGCCCTGCCACATGCACTTCAAGCAGCGCGTCGATGACGTGAAGCGCGGCGTGCTGATGGCGGGCGGGTTCCCG
ATCGAGCTGCCGGCGCTGTCGCTGTCGGAGTCGCTGCTCAAGCCGACCACGATGCTCTATCGCAACATGTTGGCGATGGA
CGTCGAGGAGCTCCTGCGCAGCCATCCCGTGGATGGCGTCGTGCTGATGGGCGGCTGCGACAAGACCACGCCGGCGCTGC
TGCTCGGCGCCACCAGCATGAACCTGCCGGCGATCTATCTCCCGGCCGGGCCGATGCTGCGCGGCAATTGGAAGGGCAAG
ACGCTCGGCTCCGGCTCGGACGCCTGGAAGTACTGGGACGAGCGCCGCGCCGGCAAGCTGTCGGATGCCGACTGGAATGC
GATGGAGGCGGGCATCGCCCGCAGCCACGGCACCTGCATGACCATGGGCACGGCGTCGACCATGACGGCGATTGCCGAGG
CGATCGGCATGACCCTGCCGGGTGCGTCGTCGATTCCCGCCGCTGACGCCGAGCATATCCGCATGAGCTCGGAATGCGGC
CGTCGCATCGTCGAGATGGTGTGGGAGGATCTGACGCCGTCGAAGATCCAGACGCGCGAAGCGTTCGAGAACGCGATCGC
AGTCGCCATGGCGATGGGCTGCTCGACCAACGCCATCATCCACGTGATCGCGCAGGCGCGCCGCGCCGGGGCCGATATCG
GCCTCGACGGTTTCGAGGCGGCGAGCCGCAAGGTGCCGGTGCTCGCCAATGTCCGGCCGAGCGGCGACACCTATCTGATG
GAAGACTTCTACTACGCCGGCGGGCTTCCCGGTCTGATGAGCCGGATGACGGAGCATCTCCATCTCGACTGCCTCACCGT
CAACGGCCGCACCCTCGGCGATAACATCAAGGGCGCCGGCGTCTATAACGACGACGTCATCCGGCCGCAGTCGAACCCGA
TCTATCGCGAGGGCGCGCTCGCCGTGCTCAAGGGCAACCTCGCCCCCGACGGCTGCGTCATCAAACCCTCTGCTTGTGAT
CCGCGCTTCCTCAAGCATACCGGCCCGGCGCTGGTGTTCGACGATTATCCGTCGATGAAGAAGGCGATCGACGATCCCGA
CCTCGACGTCACCGCCGACCACGTCATGATCCTGCGCAATGCCGGCCCGCAGGGCGGCCCCGGCATGCCGGAATGGGGTA
TGCTCCCGATCCCGACCAAGCTCGTGAAGCAGGGTGTCCGCGACATGGTGCGGCTGTCGGACGCGCGCATGAGCGGCACC
TCTTATGGCGCTTGCATCCTGCACGTCTCGCCTGAATCTTATATCGGCGGCCCCCTCGCGCTTGTGCGCAACGGCGATCT
CATCACGCTCGACGTTGCCGCTCGTACGATCAATCTCGATGTGCCCGAAGATGAACTGGCTCGCCGCCGTGCCGCCTGGA
CGCCGCCGGTACCGCGCTTCGAGCGCGGCTATGGCTGGATGTTCGCCCGCCACATCAGGCAGGCCAATGACGGCTGCGAC
TTCGACTTCCTCGAGACCGGCTTCGGCGCGCCCGTGGGCGAGCCGGCGATTTATTAG

Upstream 100 bases:

>100_bases
TCGCCGGCCTTCTCACCGTGATCCTGATCGGCCTGGTGGTCGAGAACCTGATCTTCCGCACCATCGAGCGCCGGACCGTG
CTGACCTGGGGAACGCAATC

Downstream 100 bases:

>100_bases
TTCAGCTCTCATTCCGGGACGCCGCGGAGCGGAAAGCCCGGAATCCATAACCACGATCGGGAGTATGGATTCCGGGCTCG
CGCTCCGCGCGTCCCGGAAT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSNKKTPDQLRSARWFAPDDLRSFGHRSRVMQMGYAQEEWKGRPVIAIVNTWSDAQPCHMHFKQRVDDVKRGVLMAGGFP
IELPALSLSESLLKPTTMLYRNMLAMDVEELLRSHPVDGVVLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLRGNWKGK
TLGSGSDAWKYWDERRAGKLSDADWNAMEAGIARSHGTCMTMGTASTMTAIAEAIGMTLPGASSIPAADAEHIRMSSECG
RRIVEMVWEDLTPSKIQTREAFENAIAVAMAMGCSTNAIIHVIAQARRAGADIGLDGFEAASRKVPVLANVRPSGDTYLM
EDFYYAGGLPGLMSRMTEHLHLDCLTVNGRTLGDNIKGAGVYNDDVIRPQSNPIYREGALAVLKGNLAPDGCVIKPSACD
PRFLKHTGPALVFDDYPSMKKAIDDPDLDVTADHVMILRNAGPQGGPGMPEWGMLPIPTKLVKQGVRDMVRLSDARMSGT
SYGACILHVSPESYIGGPLALVRNGDLITLDVAARTINLDVPEDELARRRAAWTPPVPRFERGYGWMFARHIRQANDGCD
FDFLETGFGAPVGEPAIY

Sequences:

>Translated_578_residues
MSNKKTPDQLRSARWFAPDDLRSFGHRSRVMQMGYAQEEWKGRPVIAIVNTWSDAQPCHMHFKQRVDDVKRGVLMAGGFP
IELPALSLSESLLKPTTMLYRNMLAMDVEELLRSHPVDGVVLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLRGNWKGK
TLGSGSDAWKYWDERRAGKLSDADWNAMEAGIARSHGTCMTMGTASTMTAIAEAIGMTLPGASSIPAADAEHIRMSSECG
RRIVEMVWEDLTPSKIQTREAFENAIAVAMAMGCSTNAIIHVIAQARRAGADIGLDGFEAASRKVPVLANVRPSGDTYLM
EDFYYAGGLPGLMSRMTEHLHLDCLTVNGRTLGDNIKGAGVYNDDVIRPQSNPIYREGALAVLKGNLAPDGCVIKPSACD
PRFLKHTGPALVFDDYPSMKKAIDDPDLDVTADHVMILRNAGPQGGPGMPEWGMLPIPTKLVKQGVRDMVRLSDARMSGT
SYGACILHVSPESYIGGPLALVRNGDLITLDVAARTINLDVPEDELARRRAAWTPPVPRFERGYGWMFARHIRQANDGCD
FDFLETGFGAPVGEPAIY
>Mature_577_residues
SNKKTPDQLRSARWFAPDDLRSFGHRSRVMQMGYAQEEWKGRPVIAIVNTWSDAQPCHMHFKQRVDDVKRGVLMAGGFPI
ELPALSLSESLLKPTTMLYRNMLAMDVEELLRSHPVDGVVLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLRGNWKGKT
LGSGSDAWKYWDERRAGKLSDADWNAMEAGIARSHGTCMTMGTASTMTAIAEAIGMTLPGASSIPAADAEHIRMSSECGR
RIVEMVWEDLTPSKIQTREAFENAIAVAMAMGCSTNAIIHVIAQARRAGADIGLDGFEAASRKVPVLANVRPSGDTYLME
DFYYAGGLPGLMSRMTEHLHLDCLTVNGRTLGDNIKGAGVYNDDVIRPQSNPIYREGALAVLKGNLAPDGCVIKPSACDP
RFLKHTGPALVFDDYPSMKKAIDDPDLDVTADHVMILRNAGPQGGPGMPEWGMLPIPTKLVKQGVRDMVRLSDARMSGTS
YGACILHVSPESYIGGPLALVRNGDLITLDVAARTINLDVPEDELARRRAAWTPPVPRFERGYGWMFARHIRQANDGCDF
DFLETGFGAPVGEPAIY

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=555, Percent_Identity=32.6126126126126, Blast_Score=265, Evalue=8e-72,
Organism=Escherichia coli, GI1786464, Length=485, Percent_Identity=31.5463917525773, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI2367371, Length=461, Percent_Identity=30.8026030368764, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1788157, Length=520, Percent_Identity=29.4230769230769, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322476, Length=547, Percent_Identity=30.7129798903108, Blast_Score=271, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62930; Mature: 62799

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKTPDQLRSARWFAPDDLRSFGHRSRVMQMGYAQEEWKGRPVIAIVNTWSDAQPCHM
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCHHHH
HFKQRVDDVKRGVLMAGGFPIELPALSLSESLLKPTTMLYRNMLAMDVEELLRSHPVDGV
HHHHHHHHHHCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLRGNWKGKTLGSGSDAWKYWDERRAGKL
EEECCCCCCCCEEEEECCCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCC
SDADWNAMEAGIARSHGTCMTMGTASTMTAIAEAIGMTLPGASSIPAADAEHIRMSSECG
CCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRIVEMVWEDLTPSKIQTREAFENAIAVAMAMGCSTNAIIHVIAQARRAGADIGLDGFEA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ASRKVPVLANVRPSGDTYLMEDFYYAGGLPGLMSRMTEHLHLDCLTVNGRTLGDNIKGAG
HCCCCCEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHHHEEEEEEECCEEECCCCCCCC
VYNDDVIRPQSNPIYREGALAVLKGNLAPDGCVIKPSACDPRFLKHTGPALVFDDYPSMK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCHHHHHCCCEEEECCCCHHH
KAIDDPDLDVTADHVMILRNAGPQGGPGMPEWGMLPIPTKLVKQGVRDMVRLSDARMSGT
HHCCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SYGACILHVSPESYIGGPLALVRNGDLITLDVAARTINLDVPEDELARRRAAWTPPVPRF
CCCEEEEEECCHHHCCCCEEEEECCCEEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCC
ERGYGWMFARHIRQANDGCDFDFLETGFGAPVGEPAIY
CCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SNKKTPDQLRSARWFAPDDLRSFGHRSRVMQMGYAQEEWKGRPVIAIVNTWSDAQPCHM
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCHHHH
HFKQRVDDVKRGVLMAGGFPIELPALSLSESLLKPTTMLYRNMLAMDVEELLRSHPVDGV
HHHHHHHHHHCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLRGNWKGKTLGSGSDAWKYWDERRAGKL
EEECCCCCCCCEEEEECCCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCC
SDADWNAMEAGIARSHGTCMTMGTASTMTAIAEAIGMTLPGASSIPAADAEHIRMSSECG
CCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRIVEMVWEDLTPSKIQTREAFENAIAVAMAMGCSTNAIIHVIAQARRAGADIGLDGFEA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ASRKVPVLANVRPSGDTYLMEDFYYAGGLPGLMSRMTEHLHLDCLTVNGRTLGDNIKGAG
HCCCCCEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHHHEEEEEEECCEEECCCCCCCC
VYNDDVIRPQSNPIYREGALAVLKGNLAPDGCVIKPSACDPRFLKHTGPALVFDDYPSMK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCHHHHHCCCEEEECCCCHHH
KAIDDPDLDVTADHVMILRNAGPQGGPGMPEWGMLPIPTKLVKQGVRDMVRLSDARMSGT
HHCCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SYGACILHVSPESYIGGPLALVRNGDLITLDVAARTINLDVPEDELARRRAAWTPPVPRF
CCCEEEEEECCHHHCCCCEEEEECCCEEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCC
ERGYGWMFARHIRQANDGCDFDFLETGFGAPVGEPAIY
CCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA