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The map label for this gene is oprF [H]

Identifier: 146343730

GI number: 146343730

Start: 7269433

End: 7270074

Strand: Reverse

Name: oprF [H]

Synonym: BRADO6977

Alternate gene names: 146343730

Gene position: 7270074-7269433 (Counterclockwise)

Preceding gene: 146343731

Following gene: 146343729

Centisome position: 97.5

GC content: 66.51

Gene sequence:

>642_bases
TTGACCGGCCTGCTGGTCGCCACGATGCTGAGCGCTTCTGCCGCGCTGATCGCCACGCAGGCTGTTGCTGCGGAGGACGT
CACGGAAGATCAGATCGTCCGTGCGCTGGCCGGACCGAAGAAGCCGCTGACCCGCGGCCTGTCGATGGCCCCGCAGGCGG
AGGCCGCGCCTGCTGCGGCCAACCCGGAGCAGGACAAGTTCCTGCAGAGCATCCGTGGCCGCGCGACGCGCTCGCTGTCG
TCTGCGGAACGCGAGGAGATCGCCGCGGTCGCCAAGACCAAGCCGAACATCGATCTCGAGATCACCTTCGACTACAACTC
GGCCAACATCAGCCAGAAATCGATGGCGTCCGTGCAGGCGCTCGGCCGCGCGCTGACCAGCCCGGACCTCAAGGGATCGA
CCTTCGTGCTCGCCGGCCATACCGACGCGGCCGGCGCCGACGCCTACAATCAGGATCTCTCGGAACGTCGTGCCGATTCG
ATCAAGCGCTATCTGGTCGAGAAGTTCGGCATCGCCGGCAGCGATCTGGTGACGGTCGGCTACGGCAAGAGCAAGCTGAA
GGATCCGTCCCAGCCGCTGGCCGAGGTCAACCGCCGCGTCCAGGTCGTCAACATGCAGAGCAAGACGGCGGCCGCCAAGT
AG

Upstream 100 bases:

>100_bases
GGGCGTTCGCGGCGAGGTGGGACATCAGTTCGCGCCAGTTGCGCGTGAGGAGGGATTGGCAATGACCGCACCGAAGACGA
ATTCGAGCAAAGCCGCCGGT

Downstream 100 bases:

>100_bases
CCTGCGCGCCGGGGACCGCGTGGCGAGATACCGGCGTCGTCACATGCGTCTCGCAGGCTTGCGGGACGCATCGTTGTGTC
GGACGCTGCCTCGCCCCATC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTGLLVATMLSASAALIATQAVAAEDVTEDQIVRALAGPKKPLTRGLSMAPQAEAAPAAANPEQDKFLQSIRGRATRSLS
SAEREEIAAVAKTKPNIDLEITFDYNSANISQKSMASVQALGRALTSPDLKGSTFVLAGHTDAAGADAYNQDLSERRADS
IKRYLVEKFGIAGSDLVTVGYGKSKLKDPSQPLAEVNRRVQVVNMQSKTAAAK

Sequences:

>Translated_213_residues
MTGLLVATMLSASAALIATQAVAAEDVTEDQIVRALAGPKKPLTRGLSMAPQAEAAPAAANPEQDKFLQSIRGRATRSLS
SAEREEIAAVAKTKPNIDLEITFDYNSANISQKSMASVQALGRALTSPDLKGSTFVLAGHTDAAGADAYNQDLSERRADS
IKRYLVEKFGIAGSDLVTVGYGKSKLKDPSQPLAEVNRRVQVVNMQSKTAAAK
>Mature_212_residues
TGLLVATMLSASAALIATQAVAAEDVTEDQIVRALAGPKKPLTRGLSMAPQAEAAPAAANPEQDKFLQSIRGRATRSLSS
AEREEIAAVAKTKPNIDLEITFDYNSANISQKSMASVQALGRALTSPDLKGSTFVLAGHTDAAGADAYNQDLSERRADSI
KRYLVEKFGIAGSDLVTVGYGKSKLKDPSQPLAEVNRRVQVVNMQSKTAAAK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22445; Mature: 22313

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLLVATMLSASAALIATQAVAAEDVTEDQIVRALAGPKKPLTRGLSMAPQAEAAPAAA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCC
NPEQDKFLQSIRGRATRSLSSAEREEIAAVAKTKPNIDLEITFDYNSANISQKSMASVQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHH
LGRALTSPDLKGSTFVLAGHTDAAGADAYNQDLSERRADSIKRYLVEKFGIAGSDLVTVG
HHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
YGKSKLKDPSQPLAEVNRRVQVVNMQSKTAAAK
CCHHHCCCCCHHHHHHHCCEEEEECCHHHCCCC
>Mature Secondary Structure 
TGLLVATMLSASAALIATQAVAAEDVTEDQIVRALAGPKKPLTRGLSMAPQAEAAPAAA
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCC
NPEQDKFLQSIRGRATRSLSSAEREEIAAVAKTKPNIDLEITFDYNSANISQKSMASVQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHH
LGRALTSPDLKGSTFVLAGHTDAAGADAYNQDLSERRADSIKRYLVEKFGIAGSDLVTVG
HHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
YGKSKLKDPSQPLAEVNRRVQVVNMQSKTAAAK
CCHHHCCCCCHHHHHHHCCEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]