Click here to switch to the map view.

The map label for this gene is mhpA [H]

Identifier: 146343722

GI number: 146343722

Start: 7254243

End: 7255874

Strand: Reverse

Name: mhpA [H]

Synonym: BRADO6969

Alternate gene names: 146343722

Gene position: 7255874-7254243 (Counterclockwise)

Preceding gene: 146343724

Following gene: 146343721

Centisome position: 97.31

GC content: 66.61

Gene sequence:

>1632_bases
ATGGTGCAGACCAGTCCGGGGCAGGGCGCAAGCTCGGCAAAGGTTCAGTTCGGCTATATCAGGCACGGTGATCAGGATCA
GGCCGCGCCGGTCGCGCATCCCGTCGTGGTGGTGGGCGCCGGTCCCGTTGGCCTGTCGTTTGCGATCGATCTCGCGCAGC
GCGGCCATGCCGTCGTCGTGCTCGACGATGCCGATCGCATCGGCGAGGGCTCGCGCGCGATCTGCTTCTCCAAGCGCTCG
CTGGATTACTGGGATCGGCTCGGCGTCGGCGACCGCATGGTCGACAAGGGCGTGGTGTGGAACGTCGGCCGCATCTTCCA
CGGCCCGTCGGAGCTCTATCAGTTCAACCTGTTGCCCGAACCCGGCCACAAGCGTCCGGCCTTCATCAACCTGCAGCAGT
TCTACGCCGAGGCCTATCTTGTCGATCGCGTCAGCGCGCTGCCGTCGATCTCGCTGCGCTGGCGCAACAAGGTGACCGCG
CTGGAACAGCGCAATGACGGTGCGGTGCTGACGATCGAGACGCCGGATGGCCCCTACCGACTGTCGGCGCAATATGTGAT
CGCCTGCGACGGTGCGCGCTCGTCGCTGCGAAGCATGGTCGGCGCCGGCTTCTCCGGAAAGGTGTTCGAGGATCAGTTCT
TGATCGCCGACGTCAAGATGACCGCCGCGTTTCCGACTGAGCGCTGGTTCTGGTTCGACCCGCCATTCCATGCCGGACGC
TCGGCCCTGCTGCATCGCCAGCCCGACGATGTCTGGCGCATCGACCTGCAGCTCACGCCGGAGTGCGATCCCCAGGTCGA
GAAGAAGCCGGAGAACGTGCGGCCGCGCATCGCGCGCATGCTCGGCCACGACAAATTCGAGTTCGAATGGATCTCGCTGT
ACAAGTTCCAGTGCCGCCGCATGGAGCGGTTCATCCATGGCCGCGTGATCTTCGCCGGCGATTCCGCCCACCAGGTGTCG
CCCTTCGGCGCGCGCGGCGCCAATTCCGGGCTGGAGGATGCGGAGAACCTGTCCTGGAAGCTCGATCGCGTGCTGCGCGG
GACGTCGCCGTCCGATTTGCTCGAGACCTATCACATCGAGCGCAGCGCCGCGGCCGACGAGAACATTCGCGAGTCCACCC
GCGCCACCGACTTCATGGCGCCGGTGAGCGCACAGGAGGCTCGGCTGCGCAAGGCGGTGCTGTCGCTCGCCAAGGAGACC
GAGTTCGGCAAGCGCATGGTCAATGGCGGCCGGCTCTCGGTACCTTCGGTGTACGAGACGCCGCTGTCGACCGCCGATGC
CGAAGCGTGGGCGAGCGGGCCTTGCCCGGGCACCTCGTTGCTCGATGCTCCGCTGAAGGCCGCCGACGGACGGCACTTTC
ATCTCACTGACGCGTTCAATGCCGAGGGCCGGCGCTTCACCGTGGTGTCATTCGCCAATGGCGCGGCGATCGACGTAGCC
AGCGAGGTCGGTGTCATCAGGATCGGCGGCGACGGTTATGTCGATGCGGAGGGATTTGCGACCAAGCGCTACGGCGCCGA
GCCCGGCACGGCCTATCTGCTCCGCCCCGACGGCTATATCGCGGCACGCTTCCGGCATCCGACGCGGGAGACCATCGCCG
CGGCCGTGGCGCGCGCCGCCGGACTGAACTGA

Upstream 100 bases:

>100_bases
GAGACTATTTGGCAAGTCGGTCGTCTGGCAAGCCAGACCAGGCCTCACCCGAGGAAATGTCGGTCGCTTCCATCGTCAAG
AACCATAAGGCGAGCAAGCC

Downstream 100 bases:

>100_bases
AGGAGCCGGTGATGGCCCTCTCGACCGCGTCGAACTTCACCAAGCCGGATGACGCCTTCCGCGCCATCGTTGAGGCGCAT
CGCGGCCTCTCGGAGAAGGA

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MVQTSPGQGASSAKVQFGYIRHGDQDQAAPVAHPVVVVGAGPVGLSFAIDLAQRGHAVVVLDDADRIGEGSRAICFSKRS
LDYWDRLGVGDRMVDKGVVWNVGRIFHGPSELYQFNLLPEPGHKRPAFINLQQFYAEAYLVDRVSALPSISLRWRNKVTA
LEQRNDGAVLTIETPDGPYRLSAQYVIACDGARSSLRSMVGAGFSGKVFEDQFLIADVKMTAAFPTERWFWFDPPFHAGR
SALLHRQPDDVWRIDLQLTPECDPQVEKKPENVRPRIARMLGHDKFEFEWISLYKFQCRRMERFIHGRVIFAGDSAHQVS
PFGARGANSGLEDAENLSWKLDRVLRGTSPSDLLETYHIERSAAADENIRESTRATDFMAPVSAQEARLRKAVLSLAKET
EFGKRMVNGGRLSVPSVYETPLSTADAEAWASGPCPGTSLLDAPLKAADGRHFHLTDAFNAEGRRFTVVSFANGAAIDVA
SEVGVIRIGGDGYVDAEGFATKRYGAEPGTAYLLRPDGYIAARFRHPTRETIAAAVARAAGLN

Sequences:

>Translated_543_residues
MVQTSPGQGASSAKVQFGYIRHGDQDQAAPVAHPVVVVGAGPVGLSFAIDLAQRGHAVVVLDDADRIGEGSRAICFSKRS
LDYWDRLGVGDRMVDKGVVWNVGRIFHGPSELYQFNLLPEPGHKRPAFINLQQFYAEAYLVDRVSALPSISLRWRNKVTA
LEQRNDGAVLTIETPDGPYRLSAQYVIACDGARSSLRSMVGAGFSGKVFEDQFLIADVKMTAAFPTERWFWFDPPFHAGR
SALLHRQPDDVWRIDLQLTPECDPQVEKKPENVRPRIARMLGHDKFEFEWISLYKFQCRRMERFIHGRVIFAGDSAHQVS
PFGARGANSGLEDAENLSWKLDRVLRGTSPSDLLETYHIERSAAADENIRESTRATDFMAPVSAQEARLRKAVLSLAKET
EFGKRMVNGGRLSVPSVYETPLSTADAEAWASGPCPGTSLLDAPLKAADGRHFHLTDAFNAEGRRFTVVSFANGAAIDVA
SEVGVIRIGGDGYVDAEGFATKRYGAEPGTAYLLRPDGYIAARFRHPTRETIAAAVARAAGLN
>Mature_543_residues
MVQTSPGQGASSAKVQFGYIRHGDQDQAAPVAHPVVVVGAGPVGLSFAIDLAQRGHAVVVLDDADRIGEGSRAICFSKRS
LDYWDRLGVGDRMVDKGVVWNVGRIFHGPSELYQFNLLPEPGHKRPAFINLQQFYAEAYLVDRVSALPSISLRWRNKVTA
LEQRNDGAVLTIETPDGPYRLSAQYVIACDGARSSLRSMVGAGFSGKVFEDQFLIADVKMTAAFPTERWFWFDPPFHAGR
SALLHRQPDDVWRIDLQLTPECDPQVEKKPENVRPRIARMLGHDKFEFEWISLYKFQCRRMERFIHGRVIFAGDSAHQVS
PFGARGANSGLEDAENLSWKLDRVLRGTSPSDLLETYHIERSAAADENIRESTRATDFMAPVSAQEARLRKAVLSLAKET
EFGKRMVNGGRLSVPSVYETPLSTADAEAWASGPCPGTSLLDAPLKAADGRHFHLTDAFNAEGRRFTVVSFANGAAIDVA
SEVGVIRIGGDGYVDAEGFATKRYGAEPGTAYLLRPDGYIAARFRHPTRETIAAAVARAAGLN

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=538, Percent_Identity=26.9516728624535, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 59661; Mature: 59661

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTSPGQGASSAKVQFGYIRHGDQDQAAPVAHPVVVVGAGPVGLSFAIDLAQRGHAVVV
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEHHHCCCEEEE
LDDADRIGEGSRAICFSKRSLDYWDRLGVGDRMVDKGVVWNVGRIFHGPSELYQFNLLPE
ECCCHHCCCCCEEEEEECCCCCHHHHCCCCCHHHCCCCEEECHHHCCCHHHHEEEECCCC
PGHKRPAFINLQQFYAEAYLVDRVSALPSISLRWRNKVTALEQRNDGAVLTIETPDGPYR
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEECCCCCEE
LSAQYVIACDGARSSLRSMVGAGFSGKVFEDQFLIADVKMTAAFPTERWFWFDPPFHAGR
EEEEEEEEECCCHHHHHHHHCCCCCCCEECCCEEEEEEEEEEECCCCCEEEECCCHHCCC
SALLHRQPDDVWRIDLQLTPECDPQVEKKPENVRPRIARMLGHDKFEFEWISLYKFQCRR
HHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHH
MERFIHGRVIFAGDSAHQVSPFGARGANSGLEDAENLSWKLDRVLRGTSPSDLLETYHIE
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
RSAAADENIRESTRATDFMAPVSAQEARLRKAVLSLAKETEFGKRMVNGGRLSVPSVYET
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCC
PLSTADAEAWASGPCPGTSLLDAPLKAADGRHFHLTDAFNAEGRRFTVVSFANGAAIDVA
CCCCCCCHHHCCCCCCCCHHHCCCHHHCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEC
SEVGVIRIGGDGYVDAEGFATKRYGAEPGTAYLLRPDGYIAARFRHPTRETIAAAVARAA
CCCCEEEECCCCEECCCCCEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHC
GLN
CCC
>Mature Secondary Structure
MVQTSPGQGASSAKVQFGYIRHGDQDQAAPVAHPVVVVGAGPVGLSFAIDLAQRGHAVVV
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEHHHCCCEEEE
LDDADRIGEGSRAICFSKRSLDYWDRLGVGDRMVDKGVVWNVGRIFHGPSELYQFNLLPE
ECCCHHCCCCCEEEEEECCCCCHHHHCCCCCHHHCCCCEEECHHHCCCHHHHEEEECCCC
PGHKRPAFINLQQFYAEAYLVDRVSALPSISLRWRNKVTALEQRNDGAVLTIETPDGPYR
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEECCCCCEE
LSAQYVIACDGARSSLRSMVGAGFSGKVFEDQFLIADVKMTAAFPTERWFWFDPPFHAGR
EEEEEEEEECCCHHHHHHHHCCCCCCCEECCCEEEEEEEEEEECCCCCEEEECCCHHCCC
SALLHRQPDDVWRIDLQLTPECDPQVEKKPENVRPRIARMLGHDKFEFEWISLYKFQCRR
HHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHH
MERFIHGRVIFAGDSAHQVSPFGARGANSGLEDAENLSWKLDRVLRGTSPSDLLETYHIE
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
RSAAADENIRESTRATDFMAPVSAQEARLRKAVLSLAKETEFGKRMVNGGRLSVPSVYET
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCC
PLSTADAEAWASGPCPGTSLLDAPLKAADGRHFHLTDAFNAEGRRFTVVSFANGAAIDVA
CCCCCCCHHHCCCCCCCCHHHCCCHHHCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEC
SEVGVIRIGGDGYVDAEGFATKRYGAEPGTAYLLRPDGYIAARFRHPTRETIAAAVARAA
CCCCEEEECCCCEECCCCCEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHC
GLN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA