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The map label for this gene is proP [H]

Identifier: 146343452

GI number: 146343452

Start: 6945432

End: 6947102

Strand: Direct

Name: proP [H]

Synonym: BRADO6681

Alternate gene names: 146343452

Gene position: 6945432-6947102 (Clockwise)

Preceding gene: 146343449

Following gene: 146343456

Centisome position: 93.14

GC content: 64.15

Gene sequence:

>1671_bases
ATGCCAATGTCAACAATGGTCGCCACGCAAGCGAGATCGGGAGGCATGACGAAGGACGAACGCTTCGTCATTCTGGCCTC
CTCTCTCGGCACCGTCTTCGAGTGGTACGATTTCTATCTCTACGGTTCGCTGGCCTCGATCATCGGCGCCCAGTTCTTCT
CGGCCTATCCCCCGGCCACGCGCGACATCTTCGCGCTGCTCGCCTTCGCCGCCGGCTTCCTGGTGCGCCCCTTCGGCGCC
ATCGTGTTCGGCCGGATCGGCGACATCGTCGGCCGCAAATACACCTTCCTGGTCACGATCCTGATCATGGGCCTGTCGAC
CTTCATCGTTGGCCTGCTGCCGAACGCCGAGACGATCGGCTTCGCCGCGCCGGTGATCCTGATCTCGCTGCGCCTGCTGC
AGGGCCTCGCGCTCGGCGGTGAATATGGCGGCGCCGCGACCTACGTCGCCGAGCACGCGCCGAACGGCAAGCGCGGCTAC
TACACCTCCTTCATCCAGACCACCGCGACGCTGGGCCTGTTTCTCTCGCTGCTGGTGATCCTGTTTACCCGCACCATCAT
CGGCGAGGCGGACTTCGCCAAGTGGGGCTGGCGCGTTCCGTTCCTGGTCTCGGTGCTGCTGCTCGGCGTTTCGCTCTGGA
TCCGCCTGCGGCTCAACGAGTCGCCGGTCTTCCAGAAGATGAAGGAAGAGGGCAAGAGCTCGAAGGCGCCGCTGACGGAA
GCCTTCGCCAATTGGAGCAATGCCAAGATCGTTCTGCTGGCGCTGATCGGCGGCACCATGGGCCAGGGCGTGGTCTGGTA
CACCGGCCAGTTCTACGCGCTGTTCTTCCTGCAATCGATCCTGAAGGTCGACGGCTACACCGCCAACCTCTTGATCGCCT
GGTCGCTGCTGCTCGGCACCGGGTTCTTCGTGATCTTCGGCATGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCATG
GGCGGTTGCCTGATCGCGGCGCTGACCTTCTTCCCGATCTTCAAGATGATCACCAGCAACGCCAACCCGGCGCTGGAGAA
GGCGATCGAGTCGGTGAAGGTGGAAGTGGTGGCCGATCCCGCCGGCTGCGGCGACCTGTTCAACCCGGTCGGCACCCGCG
TCTTCACCCAGCCCTGCGACACCGCCCGCGCCTACCTGTCCCAGCAGTCGGTGAAGTACTCGACGACCTCGGGTCCGGCT
GGCTCCGGCGTGAAGGTGACCGTCAACGGCACGGACGTGCCTTATGCTGACGCCAAGGCCGGCAATCCCGCGGTCCTCGC
GGCCGTTCAGGCCGCCGGCTATCCGAAGGCCGGCGACGCCGCGATCGTGAAGATGTCGAACCCCTTCGACATCTTCCGTC
CGCAGGTCGCCGCCATCATCGGCCTGCTGTTCATCCTGGTGATCTTCGTGACCATGGTCTACGGCCCGATCGCCGCGATG
CTGGTCGAGTTGTTCCCGACCAAGATCCGCTACACCTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGG
CCTGCTGCCGGCAACCGCCTTCGCGATCGTGGCCTCGACCGGCGATATCTACGCCGGCCTGTGGTACCCGGTCGTATTCG
CCGTGATCACTGCGATCGTCGGCTTCGTCTTCCTGCCGGAGACGAAGGACGTCAACATCCAGGACCATTGA

Upstream 100 bases:

>100_bases
TCACTGCGATGTTATCGAAGGCGCAAGGTCTCCGCGATCTCTCGCCGTCCAAGTCCGGCATGCGGAGGCGAAACCATCAT
AGCAACAACTTGGGGAGCGA

Downstream 100 bases:

>100_bases
GGTCTCTGTCACGATGCGAGCGGCACCCGCTCGCATCCACCTCGAGCGAACGGCCGCGGATCACTCCGCGGCCGTTCGGC
GTTTCAAGCCAGAGGTTTTG

Product: MFS family sugar transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPMSTMVATQARSGGMTKDERFVILASSLGTVFEWYDFYLYGSLASIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGA
IVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAETIGFAAPVILISLRLLQGLALGGEYGGAATYVAEHAPNGKRGY
YTSFIQTTATLGLFLSLLVILFTRTIIGEADFAKWGWRVPFLVSVLLLGVSLWIRLRLNESPVFQKMKEEGKSSKAPLTE
AFANWSNAKIVLLALIGGTMGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVIFGMLSDKIGRKPIIM
GGCLIAALTFFPIFKMITSNANPALEKAIESVKVEVVADPAGCGDLFNPVGTRVFTQPCDTARAYLSQQSVKYSTTSGPA
GSGVKVTVNGTDVPYADAKAGNPAVLAAVQAAGYPKAGDAAIVKMSNPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAAM
LVELFPTKIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPVVFAVITAIVGFVFLPETKDVNIQDH

Sequences:

>Translated_556_residues
MPMSTMVATQARSGGMTKDERFVILASSLGTVFEWYDFYLYGSLASIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGA
IVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAETIGFAAPVILISLRLLQGLALGGEYGGAATYVAEHAPNGKRGY
YTSFIQTTATLGLFLSLLVILFTRTIIGEADFAKWGWRVPFLVSVLLLGVSLWIRLRLNESPVFQKMKEEGKSSKAPLTE
AFANWSNAKIVLLALIGGTMGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVIFGMLSDKIGRKPIIM
GGCLIAALTFFPIFKMITSNANPALEKAIESVKVEVVADPAGCGDLFNPVGTRVFTQPCDTARAYLSQQSVKYSTTSGPA
GSGVKVTVNGTDVPYADAKAGNPAVLAAVQAAGYPKAGDAAIVKMSNPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAAM
LVELFPTKIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPVVFAVITAIVGFVFLPETKDVNIQDH
>Mature_555_residues
PMSTMVATQARSGGMTKDERFVILASSLGTVFEWYDFYLYGSLASIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAI
VFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAETIGFAAPVILISLRLLQGLALGGEYGGAATYVAEHAPNGKRGYY
TSFIQTTATLGLFLSLLVILFTRTIIGEADFAKWGWRVPFLVSVLLLGVSLWIRLRLNESPVFQKMKEEGKSSKAPLTEA
FANWSNAKIVLLALIGGTMGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGTGFFVIFGMLSDKIGRKPIIMG
GCLIAALTFFPIFKMITSNANPALEKAIESVKVEVVADPAGCGDLFNPVGTRVFTQPCDTARAYLSQQSVKYSTTSGPAG
SGVKVTVNGTDVPYADAKAGNPAVLAAVQAAGYPKAGDAAIVKMSNPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAAML
VELFPTKIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPVVFAVITAIVGFVFLPETKDVNIQDH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=333, Percent_Identity=35.7357357357357, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788292, Length=351, Percent_Identity=33.048433048433, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788942, Length=349, Percent_Identity=32.378223495702, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789941, Length=338, Percent_Identity=35.5029585798817, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082404, Length=327, Percent_Identity=24.4648318042813, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59733; Mature: 59602

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSTMVATQARSGGMTKDERFVILASSLGTVFEWYDFYLYGSLASIIGAQFFSAYPPAT
CCCHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RDIFALLAFAAGFLVRPFGAIVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAETIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FAAPVILISLRLLQGLALGGEYGGAATYVAEHAPNGKRGYYTSFIQTTATLGLFLSLLVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LFTRTIIGEADFAKWGWRVPFLVSVLLLGVSLWIRLRLNESPVFQKMKEEGKSSKAPLTE
HHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCHHH
AFANWSNAKIVLLALIGGTMGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGT
HHCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GFFVIFGMLSDKIGRKPIIMGGCLIAALTFFPIFKMITSNANPALEKAIESVKVEVVADP
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEECC
AGCGDLFNPVGTRVFTQPCDTARAYLSQQSVKYSTTSGPAGSGVKVTVNGTDVPYADAKA
CCCCHHHHHHCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCCCCC
GNPAVLAAVQAAGYPKAGDAAIVKMSNPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAAM
CCCHHEEEHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVELFPTKIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPVVFAVITAIV
HHHHCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GFVFLPETKDVNIQDH
HHEECCCCCCCCCCCC
>Mature Secondary Structure 
PMSTMVATQARSGGMTKDERFVILASSLGTVFEWYDFYLYGSLASIIGAQFFSAYPPAT
CCHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RDIFALLAFAAGFLVRPFGAIVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAETIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FAAPVILISLRLLQGLALGGEYGGAATYVAEHAPNGKRGYYTSFIQTTATLGLFLSLLVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LFTRTIIGEADFAKWGWRVPFLVSVLLLGVSLWIRLRLNESPVFQKMKEEGKSSKAPLTE
HHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCHHH
AFANWSNAKIVLLALIGGTMGQGVVWYTGQFYALFFLQSILKVDGYTANLLIAWSLLLGT
HHCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GFFVIFGMLSDKIGRKPIIMGGCLIAALTFFPIFKMITSNANPALEKAIESVKVEVVADP
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEECC
AGCGDLFNPVGTRVFTQPCDTARAYLSQQSVKYSTTSGPAGSGVKVTVNGTDVPYADAKA
CCCCHHHHHHCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCCCCC
GNPAVLAAVQAAGYPKAGDAAIVKMSNPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAAM
CCCHHEEEHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVELFPTKIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPVVFAVITAIV
HHHHCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GFVFLPETKDVNIQDH
HHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]