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The map label for this gene is nuoL [H]

Identifier: 146343036

GI number: 146343036

Start: 6465647

End: 6467233

Strand: Reverse

Name: nuoL [H]

Synonym: BRADO6224

Alternate gene names: 146343036

Gene position: 6467233-6465647 (Counterclockwise)

Preceding gene: 146343038

Following gene: 146343035

Centisome position: 86.73

GC content: 70.51

Gene sequence:

>1587_bases
ATGACCCAGCTCCTTCCCTGCCTCGCGGCGCTTGCGCCGCTGACCCTTGCCCTGGTCGCCCTCCTCGGCGCGGAGGCCGG
CGCCCGGGCGGTGGCCTCCCGCGCCATCGGCGCAGCCGCGATCGCCTTCGCCATCGCCACGACGGCGAGCCTCGCCGCAG
CCGTGCTCGGGGCAGGGCGCACGCCGCTGCTCGGCGCCTCGGGGCTCGGCCTGTCCCTGCAGCTCGATGCGCTCGGCGCG
ATTCTCTCCATGCTGGTCAGCTTCATCGGCCTGGTCGTGGTTCGCTACAGCCGCAACTATCTCGATGGCGATCCCGGCCA
GGTCCGGTTCACGCGCTGGCTGCTGCTGACGCTTGCCGCGGTGTTGATGCTGCCGATCGCGGGCAATGTGGTGCTGCTGT
TCGCCGCCTGGGTCGCGACCAGCCTGGCGCTCGGCAAGCTGCTCTTGTTCTACGAGACCCGGCCGGCCGCGATCCGCGCC
TCGCGCAAGAAGTTCGTGGCGAGCCGGCTCGGCGACGCCTTCCTCGCCCTCGCGGCGCTGCTGCTCTACATCCGGTTCGG
CACGCTGGAGATCGCGGGCCTCGCGGCTCACGCCCGGGCGGGCGAGGGCGCCGGCTCGGCGATGGTGACGGTCGCGGCGC
TCCTGATCGTCGCGACGGCAATGCTGAAATCGGCGCAGCTGCCGCTGCATGGCTGGCTGATCGAGGTGATGGAGACGCCG
ACGCCGGTGTCGGCGCTGCTGCATGCCGGCATCATCAATGCCGGCGGCTTCCTGGTGCTCCGGCTCAGCGATGTGCTGCT
GCTGGCGACGCCCGCGCTCGACGTGCTCGCCGTGGTCGGCGGCGCCACCGCGCTGTTCGGCTCGCTTGTGATGCTGACGC
AGACCTCGGTGAAGGTGCAGCTGGCCTATTCGACGGTGGCCCAGATGGGCTTCATGCTGCTGCAATGCGGGCTCGGCGCA
TTCTCGGCGGCGCTGCTGCACATCGTGGCGCACTCGCTCTACAAGGCGCACGCCTTCCTGTCCTCGGGCAGCATCATCGA
CCTCGCCCGGGCCTCCTGGACGCCGAGCCCCGGCGGCCAGCCGCATGCGGCACGGCTGATCCTGGCGCTGGTCGCAGTCA
TCGGCCTGGCGCTGCTGACCGGCCTCGCCTTCGGCATGACGCTCAGGAGCAACCCGGCGCAGATCGCGCTCGGTTCGGTG
CTGCTGATGGGGCTGGTGCACCTGATGGCCAATGCCATCGACGAGCGGCCGAATGCCTTCGTCATCGCCCGTGCCGCGGT
CACTGCAGCCGGCGTTGCAATCGTGTACTTCGCCCTGCAGGCCGGCACGGCCTGGCTGGTCGGCGGCAGCATCCCCGCCG
CGCGGCCGGTCGACGGCGTCGTGCCGATGCTGATCGCGGCGGCGGTGGTGATCTCGTTCGCCGCGGTGACCGTGTTCCAG
AACCAGATGATGCGCCGCGCCGACGCGCCGTTCTGGATCGCGGCCTATGTCCACCTACGCAACGGCCTCTACCTCAACAC
GCTTGCCAACCGCCTCGTCCTGACCGTGTGGCCGCGCAATTCCGCGACGTCTTCCTCCAACCCGTGA

Upstream 100 bases:

>100_bases
TTTTCTAACGATCATGTTCAATGATTGAAATTTTAAGAAGGCTTCGGGGCCGTTAAAGACAAGCAGCAACCGTCTCATTT
GATCAAACCTCCGAGCCGCC

Downstream 100 bases:

>100_bases
TCCGAGACCTCACATGACCCAGGCCGCCCTCGCCATGTCCATGACCGACCCGATCGACACCACCGCCGATGCCGGGCTGA
TGGCCCGCATCGACAGCGCC

Product: putative NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTQLLPCLAALAPLTLALVALLGAEAGARAVASRAIGAAAIAFAIATTASLAAAVLGAGRTPLLGASGLGLSLQLDALGA
ILSMLVSFIGLVVVRYSRNYLDGDPGQVRFTRWLLLTLAAVLMLPIAGNVVLLFAAWVATSLALGKLLLFYETRPAAIRA
SRKKFVASRLGDAFLALAALLLYIRFGTLEIAGLAAHARAGEGAGSAMVTVAALLIVATAMLKSAQLPLHGWLIEVMETP
TPVSALLHAGIINAGGFLVLRLSDVLLLATPALDVLAVVGGATALFGSLVMLTQTSVKVQLAYSTVAQMGFMLLQCGLGA
FSAALLHIVAHSLYKAHAFLSSGSIIDLARASWTPSPGGQPHAARLILALVAVIGLALLTGLAFGMTLRSNPAQIALGSV
LLMGLVHLMANAIDERPNAFVIARAAVTAAGVAIVYFALQAGTAWLVGGSIPAARPVDGVVPMLIAAAVVISFAAVTVFQ
NQMMRRADAPFWIAAYVHLRNGLYLNTLANRLVLTVWPRNSATSSSNP

Sequences:

>Translated_528_residues
MTQLLPCLAALAPLTLALVALLGAEAGARAVASRAIGAAAIAFAIATTASLAAAVLGAGRTPLLGASGLGLSLQLDALGA
ILSMLVSFIGLVVVRYSRNYLDGDPGQVRFTRWLLLTLAAVLMLPIAGNVVLLFAAWVATSLALGKLLLFYETRPAAIRA
SRKKFVASRLGDAFLALAALLLYIRFGTLEIAGLAAHARAGEGAGSAMVTVAALLIVATAMLKSAQLPLHGWLIEVMETP
TPVSALLHAGIINAGGFLVLRLSDVLLLATPALDVLAVVGGATALFGSLVMLTQTSVKVQLAYSTVAQMGFMLLQCGLGA
FSAALLHIVAHSLYKAHAFLSSGSIIDLARASWTPSPGGQPHAARLILALVAVIGLALLTGLAFGMTLRSNPAQIALGSV
LLMGLVHLMANAIDERPNAFVIARAAVTAAGVAIVYFALQAGTAWLVGGSIPAARPVDGVVPMLIAAAVVISFAAVTVFQ
NQMMRRADAPFWIAAYVHLRNGLYLNTLANRLVLTVWPRNSATSSSNP
>Mature_527_residues
TQLLPCLAALAPLTLALVALLGAEAGARAVASRAIGAAAIAFAIATTASLAAAVLGAGRTPLLGASGLGLSLQLDALGAI
LSMLVSFIGLVVVRYSRNYLDGDPGQVRFTRWLLLTLAAVLMLPIAGNVVLLFAAWVATSLALGKLLLFYETRPAAIRAS
RKKFVASRLGDAFLALAALLLYIRFGTLEIAGLAAHARAGEGAGSAMVTVAALLIVATAMLKSAQLPLHGWLIEVMETPT
PVSALLHAGIINAGGFLVLRLSDVLLLATPALDVLAVVGGATALFGSLVMLTQTSVKVQLAYSTVAQMGFMLLQCGLGAF
SAALLHIVAHSLYKAHAFLSSGSIIDLARASWTPSPGGQPHAARLILALVAVIGLALLTGLAFGMTLRSNPAQIALGSVL
LMGLVHLMANAIDERPNAFVIARAAVTAAGVAIVYFALQAGTAWLVGGSIPAARPVDGVVPMLIAAAVVISFAAVTVFQN
QMMRRADAPFWIAAYVHLRNGLYLNTLANRLVLTVWPRNSATSSSNP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=288, Percent_Identity=31.9444444444444, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1788614, Length=324, Percent_Identity=35.1851851851852, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788829, Length=339, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=26.2626262626263, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54372; Mature: 54241

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLLPCLAALAPLTLALVALLGAEAGARAVASRAIGAAAIAFAIATTASLAAAVLGAGR
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPLLGASGLGLSLQLDALGAILSMLVSFIGLVVVRYSRNYLDGDPGQVRFTRWLLLTLAA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VLMLPIAGNVVLLFAAWVATSLALGKLLLFYETRPAAIRASRKKFVASRLGDAFLALAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLYIRFGTLEIAGLAAHARAGEGAGSAMVTVAALLIVATAMLKSAQLPLHGWLIEVMETP
HHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
TPVSALLHAGIINAGGFLVLRLSDVLLLATPALDVLAVVGGATALFGSLVMLTQTSVKVQ
CHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCEEEE
LAYSTVAQMGFMLLQCGLGAFSAALLHIVAHSLYKAHAFLSSGSIIDLARASWTPSPGGQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCC
PHAARLILALVAVIGLALLTGLAFGMTLRSNPAQIALGSVLLMGLVHLMANAIDERPNAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VIARAAVTAAGVAIVYFALQAGTAWLVGGSIPAARPVDGVVPMLIAAAVVISFAAVTVFQ
EEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NQMMRRADAPFWIAAYVHLRNGLYLNTLANRLVLTVWPRNSATSSSNP
HHHHHHCCCCHHHHHHHHHHCCEEHHHHHCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TQLLPCLAALAPLTLALVALLGAEAGARAVASRAIGAAAIAFAIATTASLAAAVLGAGR
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPLLGASGLGLSLQLDALGAILSMLVSFIGLVVVRYSRNYLDGDPGQVRFTRWLLLTLAA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VLMLPIAGNVVLLFAAWVATSLALGKLLLFYETRPAAIRASRKKFVASRLGDAFLALAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLYIRFGTLEIAGLAAHARAGEGAGSAMVTVAALLIVATAMLKSAQLPLHGWLIEVMETP
HHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
TPVSALLHAGIINAGGFLVLRLSDVLLLATPALDVLAVVGGATALFGSLVMLTQTSVKVQ
CHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCEEEE
LAYSTVAQMGFMLLQCGLGAFSAALLHIVAHSLYKAHAFLSSGSIIDLARASWTPSPGGQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCC
PHAARLILALVAVIGLALLTGLAFGMTLRSNPAQIALGSVLLMGLVHLMANAIDERPNAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VIARAAVTAAGVAIVYFALQAGTAWLVGGSIPAARPVDGVVPMLIAAAVVISFAAVTVFQ
EEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NQMMRRADAPFWIAAYVHLRNGLYLNTLANRLVLTVWPRNSATSSSNP
HHHHHHCCCCHHHHHHHHHHCCEEHHHHHCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]