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The map label for this gene is yafC [C]

Identifier: 146343004

GI number: 146343004

Start: 6417924

End: 6418838

Strand: Reverse

Name: yafC [C]

Synonym: BRADO6189

Alternate gene names: 146343004

Gene position: 6418838-6417924 (Counterclockwise)

Preceding gene: 146343010

Following gene: 146343003

Centisome position: 86.08

GC content: 68.09

Gene sequence:

>915_bases
ATGGATCTGCAGGCGATCCGCATCTTCGTCGAGGTCTTGCGTCTCGGCAGCTTTGCCGCCGTGGCGCGGCAGCATGCGGT
CGATCCATCGACCGTGTCGCGCACCGTCGCGGTGCTCGAGGAGCAACTGGGTTTCAGGCTGTTGCAGCGCTCGACGCGCC
GGCTGACCGCCACCGAGGCCGGTGTCCGGTATTTCGAGCGCGTTCAACCGCTGCTCGACGAGCTCGACCGCGCCGAAAGC
GAAGCGCGCGATATCGTCGCCGCGCCGTCCGGCCGTTTGCGCGTCACCGCGTCGGTGGCGTTCGGCGCGACCGTCGTCGT
GCCCCGGCTCGCGCGACTGCGGGCGCTGCATCCGGCCCTCGACATCGAATTGATGTTGAGCGATCAGATCGTCGATGTGG
TCGCGGAGCGGATCGATATCGCGATCAGGCTGGGCGCGCGGCTCGACACCGGCCTGGTGGGCACGCGGCTGATGCAGACG
CGCTATCATGTCTGCGCCAGCCCGGACTACATTGCGCGGAACGGCAAGCCGGCGCGCCCCGAGCGTCTCGAAGACCATGA
CTGCCTGCTGTTTCCCTATCAGGGCTTCCGCACGCAATGGCGCTTTCGCAGCCGGGGCGGTGCGGTCACCGAGGTGCCGG
TGAAGGGCGGGATCGTGATCTCGAATGCGCTCGCCCTGCACCGCGCGGCGCTGGAAGGGCTTGGACCGGTGCTGCTGGCG
GACTGGCTGGTCGGCGCCGACCTGAAGCACGGCCGGCTGGTCAACCTGTTTCCGGGCTACGAGGTCACCGCCACCGCGTT
CAGCACGGCCGCGTGGATCCTCTATCCGTCGCGCAGCTACGTCCCGCTCAAGGTACGCGCCTTCATCGACTTCATGAAGG
CAGAAACCTCCGCGAAGGGGGCGCGCCGGGCTTGA

Upstream 100 bases:

>100_bases
CCGATGTTGGACCGGCTGCATCCTGCGGCCAACGACCCAGGCGGAAAAATCGAGTATATTGCATTTTAATCATGCATCTT
CCGCACGAATGGGGTCCGAC

Downstream 100 bases:

>100_bases
CGGTCGTGGCGGCGCACGCGCGCGGCCATCTCCGCGCGGAGCATTGATCGTCAGCGCCTCCAGACGAAGGGCCTTGAAAC
GGGTCGCCCGTTACGGCTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDLQAIRIFVEVLRLGSFAAVARQHAVDPSTVSRTVAVLEEQLGFRLLQRSTRRLTATEAGVRYFERVQPLLDELDRAES
EARDIVAAPSGRLRVTASVAFGATVVVPRLARLRALHPALDIELMLSDQIVDVVAERIDIAIRLGARLDTGLVGTRLMQT
RYHVCASPDYIARNGKPARPERLEDHDCLLFPYQGFRTQWRFRSRGGAVTEVPVKGGIVISNALALHRAALEGLGPVLLA
DWLVGADLKHGRLVNLFPGYEVTATAFSTAAWILYPSRSYVPLKVRAFIDFMKAETSAKGARRA

Sequences:

>Translated_304_residues
MDLQAIRIFVEVLRLGSFAAVARQHAVDPSTVSRTVAVLEEQLGFRLLQRSTRRLTATEAGVRYFERVQPLLDELDRAES
EARDIVAAPSGRLRVTASVAFGATVVVPRLARLRALHPALDIELMLSDQIVDVVAERIDIAIRLGARLDTGLVGTRLMQT
RYHVCASPDYIARNGKPARPERLEDHDCLLFPYQGFRTQWRFRSRGGAVTEVPVKGGIVISNALALHRAALEGLGPVLLA
DWLVGADLKHGRLVNLFPGYEVTATAFSTAAWILYPSRSYVPLKVRAFIDFMKAETSAKGARRA
>Mature_304_residues
MDLQAIRIFVEVLRLGSFAAVARQHAVDPSTVSRTVAVLEEQLGFRLLQRSTRRLTATEAGVRYFERVQPLLDELDRAES
EARDIVAAPSGRLRVTASVAFGATVVVPRLARLRALHPALDIELMLSDQIVDVVAERIDIAIRLGARLDTGLVGTRLMQT
RYHVCASPDYIARNGKPARPERLEDHDCLLFPYQGFRTQWRFRSRGGAVTEVPVKGGIVISNALALHRAALEGLGPVLLA
DWLVGADLKHGRLVNLFPGYEVTATAFSTAAWILYPSRSYVPLKVRAFIDFMKAETSAKGARRA

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=302, Percent_Identity=34.1059602649007, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=32.8767123287671, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=28.6184210526316, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.5918367346939, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=28.0276816608997, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=29.5138888888889, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=28.4722222222222, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789173, Length=266, Percent_Identity=27.8195488721804, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790011, Length=276, Percent_Identity=24.2753623188406, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=25.4180602006689, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=24.6710526315789, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=147, Percent_Identity=34.0136054421769, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33577; Mature: 33577

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQAIRIFVEVLRLGSFAAVARQHAVDPSTVSRTVAVLEEQLGFRLLQRSTRRLTATEA
CCHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVRYFERVQPLLDELDRAESEARDIVAAPSGRLRVTASVAFGATVVVPRLARLRALHPAL
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
DIELMLSDQIVDVVAERIDIAIRLGARLDTGLVGTRLMQTRYHVCASPDYIARNGKPARP
CEEEEEHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
ERLEDHDCLLFPYQGFRTQWRFRSRGGAVTEVPVKGGIVISNALALHRAALEGLGPVLLA
CCCCCCCEEEEECCCCCHHEEECCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCHHHHH
DWLVGADLKHGRLVNLFPGYEVTATAFSTAAWILYPSRSYVPLKVRAFIDFMKAETSAKG
HHHHCCCCCCCEEEEECCCCEEEHHHHCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCC
ARRA
CCCC
>Mature Secondary Structure
MDLQAIRIFVEVLRLGSFAAVARQHAVDPSTVSRTVAVLEEQLGFRLLQRSTRRLTATEA
CCHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVRYFERVQPLLDELDRAESEARDIVAAPSGRLRVTASVAFGATVVVPRLARLRALHPAL
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
DIELMLSDQIVDVVAERIDIAIRLGARLDTGLVGTRLMQTRYHVCASPDYIARNGKPARP
CEEEEEHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
ERLEDHDCLLFPYQGFRTQWRFRSRGGAVTEVPVKGGIVISNALALHRAALEGLGPVLLA
CCCCCCCEEEEECCCCCHHEEECCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCHHHHH
DWLVGADLKHGRLVNLFPGYEVTATAFSTAAWILYPSRSYVPLKVRAFIDFMKAETSAKG
HHHHCCCCCCCEEEEECCCCEEEHHHHCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCC
ARRA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]