Click here to switch to the map view.

The map label for this gene is nylB [H]

Identifier: 146343002

GI number: 146343002

Start: 6414717

End: 6415838

Strand: Reverse

Name: nylB [H]

Synonym: BRADO6186

Alternate gene names: 146343002

Gene position: 6415838-6414717 (Counterclockwise)

Preceding gene: 146343003

Following gene: 146342999

Centisome position: 86.04

GC content: 62.66

Gene sequence:

>1122_bases
TTGCTCGCGTTCAATCCGCTCCCGTCTCGCGCCGAGCAGGCGGATTGCGGCACGCCAGCGGCCCTGGACGACGGCTGGAC
GATCGCGAGGCCGGACGAGGTGGGCCTCGATGGGGCGCGTCTGTGCGCGATCGCCGAGCGGCTCAGGGTGACGAATGCGA
ACGTTCATGGCGTGGTGATCGTCCGCCGCGGCAAGCTGGTGTTCGAGCAGTATTTCGCAGGCTACGATGAACCTTGGGGC
GGTGATTCAGGGCGCTACGAATTCAAGGCCACGACGATGCACGACCTGCGCTCGATCACGAAGAGCGTCGTGTCGCTCCT
AGTGGGGATCGCGCTTGATCGCAAGCTGATCGAAAGCCTCGACGAGCCCGTCACCAAATACGTCCCGGACTATTCGGATG
TGAAGGCAGCTGGCTGGGACAGGATCACGCTGCGGCATCTGATCAAGATGTCGTCCGGCCTGCAATGGAATGAAAACCTG
CCGTGGGACGACAAGAATGACGAATGGCATCTGGTGAATGATTCGGACCCGCTGCGTTACGTGATGCAGAAGCCGTTCGT
TTTCGAGCCGGGCACGTTCTTCACCTACAATGGCGGGGGGACCGAGCTCCTCGGCAAGGTCATCGAAAAAGCGTCCGGGA
AACGGATCGACGTGTTCGCCGACGAGGTGCTCTTCAAACCGCTCGGGATCACCGATTGGCAGTGGAAGGACTATCGCAAC
GGCCATCTGGCCAAGGCCTCCGGGCTGCGCCTGCGTCCGCGCGATGCCGCAAAGATAGGGCAGCTGATTCTTGACGGCGG
CGAGTGGCAGGGGCGCCGTATCCTCCCCTCCGCGTGGATCGCCGAATCGATCAGGCCCCGGCTTCAGGCGACGAACATGT
TCGGTGGCCTGTTCTTTTATGGCTATCAATGGTGGATCGGCCGCTCGCTCGTCAACGAGCGAGCGGTCACGTGGATCGCC
GGTCAGGGCCTGGGCGGGCAGCGATTGGTCATCGCTCCAGCCGAACAACTCGTGATGATGGTCACCCAGGGCCTCTATGG
CAGCGCGCGGCAGGGGCAGGCGACGTTCGATCTGCTGGCCAACTTCGTTCTGCCGACGGAGCTTATCGAGGGGCCTCGCT
GA

Upstream 100 bases:

>100_bases
GAGACCATTTTGGTGTAGTGTCTCGGCCAAGTCAGAACTGTCCCATCTTGCCAGGAATGGAGCAAACCGCATGCGACTCC
AGATTGTTGCGCTGGCAGCA

Downstream 100 bases:

>100_bases
CGGCCTGCTGCGACAGAGCCGATATGGCAGATAGTCCAGCGGGCTCGGTCGAACTGCGACGCCCCCCGCTCAGAAAAAGC
CGGGACTGAGGTCGACGCCG

Product: putative beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLAFNPLPSRAEQADCGTPAALDDGWTIARPDEVGLDGARLCAIAERLRVTNANVHGVVIVRRGKLVFEQYFAGYDEPWG
GDSGRYEFKATTMHDLRSITKSVVSLLVGIALDRKLIESLDEPVTKYVPDYSDVKAAGWDRITLRHLIKMSSGLQWNENL
PWDDKNDEWHLVNDSDPLRYVMQKPFVFEPGTFFTYNGGGTELLGKVIEKASGKRIDVFADEVLFKPLGITDWQWKDYRN
GHLAKASGLRLRPRDAAKIGQLILDGGEWQGRRILPSAWIAESIRPRLQATNMFGGLFFYGYQWWIGRSLVNERAVTWIA
GQGLGGQRLVIAPAEQLVMMVTQGLYGSARQGQATFDLLANFVLPTELIEGPR

Sequences:

>Translated_373_residues
MLAFNPLPSRAEQADCGTPAALDDGWTIARPDEVGLDGARLCAIAERLRVTNANVHGVVIVRRGKLVFEQYFAGYDEPWG
GDSGRYEFKATTMHDLRSITKSVVSLLVGIALDRKLIESLDEPVTKYVPDYSDVKAAGWDRITLRHLIKMSSGLQWNENL
PWDDKNDEWHLVNDSDPLRYVMQKPFVFEPGTFFTYNGGGTELLGKVIEKASGKRIDVFADEVLFKPLGITDWQWKDYRN
GHLAKASGLRLRPRDAAKIGQLILDGGEWQGRRILPSAWIAESIRPRLQATNMFGGLFFYGYQWWIGRSLVNERAVTWIA
GQGLGGQRLVIAPAEQLVMMVTQGLYGSARQGQATFDLLANFVLPTELIEGPR
>Mature_373_residues
MLAFNPLPSRAEQADCGTPAALDDGWTIARPDEVGLDGARLCAIAERLRVTNANVHGVVIVRRGKLVFEQYFAGYDEPWG
GDSGRYEFKATTMHDLRSITKSVVSLLVGIALDRKLIESLDEPVTKYVPDYSDVKAAGWDRITLRHLIKMSSGLQWNENL
PWDDKNDEWHLVNDSDPLRYVMQKPFVFEPGTFFTYNGGGTELLGKVIEKASGKRIDVFADEVLFKPLGITDWQWKDYRN
GHLAKASGLRLRPRDAAKIGQLILDGGEWQGRRILPSAWIAESIRPRLQATNMFGGLFFYGYQWWIGRSLVNERAVTWIA
GQGLGGQRLVIAPAEQLVMMVTQGLYGSARQGQATFDLLANFVLPTELIEGPR

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 41770; Mature: 41770

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFNPLPSRAEQADCGTPAALDDGWTIARPDEVGLDGARLCAIAERLRVTNANVHGVVI
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
VRRGKLVFEQYFAGYDEPWGGDSGRYEFKATTMHDLRSITKSVVSLLVGIALDRKLIESL
EECCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEPVTKYVPDYSDVKAAGWDRITLRHLIKMSSGLQWNENLPWDDKNDEWHLVNDSDPLRY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
VMQKPFVFEPGTFFTYNGGGTELLGKVIEKASGKRIDVFADEVLFKPLGITDWQWKDYRN
HHCCCEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCC
GHLAKASGLRLRPRDAAKIGQLILDGGEWQGRRILPSAWIAESIRPRLQATNMFGGLFFY
CCEEECCCCEECCHHHHHHHHHEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
GYQWWIGRSLVNERAVTWIAGQGLGGQRLVIAPAEQLVMMVTQGLYGSARQGQATFDLLA
HHHHHHHHHHHHHHHEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHH
NFVLPTELIEGPR
HHHCCHHHHCCCC
>Mature Secondary Structure
MLAFNPLPSRAEQADCGTPAALDDGWTIARPDEVGLDGARLCAIAERLRVTNANVHGVVI
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
VRRGKLVFEQYFAGYDEPWGGDSGRYEFKATTMHDLRSITKSVVSLLVGIALDRKLIESL
EECCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEPVTKYVPDYSDVKAAGWDRITLRHLIKMSSGLQWNENLPWDDKNDEWHLVNDSDPLRY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
VMQKPFVFEPGTFFTYNGGGTELLGKVIEKASGKRIDVFADEVLFKPLGITDWQWKDYRN
HHCCCEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCC
GHLAKASGLRLRPRDAAKIGQLILDGGEWQGRRILPSAWIAESIRPRLQATNMFGGLFFY
CCEEECCCCEECCHHHHHHHHHEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
GYQWWIGRSLVNERAVTWIAGQGLGGQRLVIAPAEQLVMMVTQGLYGSARQGQATFDLLA
HHHHHHHHHHHHHHHEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHH
NFVLPTELIEGPR
HHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]