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The map label for this gene is murD [H]

Identifier: 146342500

GI number: 146342500

Start: 5854930

End: 5856330

Strand: Reverse

Name: murD [H]

Synonym: BRADO5661

Alternate gene names: 146342500

Gene position: 5856330-5854930 (Counterclockwise)

Preceding gene: 146342501

Following gene: 146342499

Centisome position: 78.54

GC content: 68.52

Gene sequence:

>1401_bases
ATGATTCCCGTCACCTCTTTCGCCGGCAAGACAGTCGCCGTGTTCGGGCTCGGCGGCTCCGGGTTGGCCTCATGCCACGC
GCTGCGCGCCGGCGGTGCCGAAGTAATCGCCGCCGATGACGGCGCCGATCGCATGACGGAGGCTGCAAAGGCGGGCTTCA
TCACCGCCGATCTGCGCACCGTCTCCTGGTCGAACTTCGCTGCGCTGGTGCTGGCGCCGGGCGTGCCGCTGACCCATCCG
GTGCCGCATTGGAGCGTGCTGAGGGCGCGCGAGGCCGGCGTCGAGGTGATCGGCGACATCGAGCTGTTCTGCCGTGAGCG
CCGGCGCCACGCGCCGGATGCGCCGTTCATCGCCATCACCGGCACCAACGGCAAGTCGACGACCACCGCGCTGATCGCGC
ATCTGATGCGGGTGGCCGGCTACGACACCCAGATGGGCGGCAATATCGGCACCGCGATTCTGTCGCTGGAGCCGCCGCGC
ATGGGGCGCGTTCATGTGATCGAGATGTCGTCCTACCAGATCGACCTGACACCGTCGCTCGATCCGAGCATCGGGATCCT
GCTCAATGTCAGCGAGGATCACATCGACCGTCACGGCACGATCGAGAACTATGCGGCGGTCAAGGAGCGCCTGGTCGCCG
GCGTGAAGCCGCAGGGCACCGCGATCGTCGGCGTCGACGACGGCTTCTGCCGCGCCATCGCCGACCGGCTCGACCAGGCC
GGCAAGCGCGTGGTCCGTATCTCGGTGAAGAACCCGCTGGCCGATGGGCTCTACACCGAGCACGAGACCATCGTACGCAG
CTCCGGCGGCGCGCGCAGCGAGGTCGCGCGGCTCGGCGGCATCGGCTCGCTGCGCGGCCAGCACAATGCGCAGAACGCGG
CCTGCGCCGCCGCGGCCGCGCTCGCGATCGGCGTGAGAGAGGATGTGCTGCAGCAAGGCCTACGCAGCTTCCCCGGCCTC
GCGCATCGCATGGAGCAGGTCGGGCGTCTCGGCAACGTGCTGTTCGTCAACGACTCCAAGGGCACCAATGCGGACGCCAC
GGCGCACGCGCTGTCGTCCTTCGCCGATATCTTCTGGATTGCCGGCGGCAAGCCCAAGGCGGGCGGCATCACCAGCCTCG
CCGGCTTCTTCCCGCGCATCCGCAAGGCCTATCTGATCGGCGAAGCGGCCAAGGAATTCGCCGGTACGCTCGGTGAGAAG
GTGCCGCACGCGATGTCCGGCACGCTGGAGGTCGCCGTGGAGCAGGCGGCTGCCGACGCCAAGGCATCAGGCTTGAAGGA
GGCCGTCGTCCTGTTGTCCCCGGCCTGCGCCTCGTTCGACCAGTTCCGCAACTTCGAGATCCGCGGCACCCGCTTTCGCG
ACCTGGTCCAGGCGCTGCCGGGCGTGGAAAAGGTCGTCTAG

Upstream 100 bases:

>100_bases
CCTCAATCCCACTGTCATTCCGGGGCGCGCGGAGCGCGAACCCGGAATCTCGAGATTCCGGATCACCGCTGCGCGGTGTC
CGAATGACGGAGGTCTTCGC

Downstream 100 bases:

>100_bases
GCTGCAAACTCCGCTGTCGTCGGCGCCGCGACGCGCTGGCGCGCGTCACGGCTTGTCCGGGACGACAGCGAGATTCTTAA
CCCTTCGATAACCATCCTGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIPVTSFAGKTVAVFGLGGSGLASCHALRAGGAEVIAADDGADRMTEAAKAGFITADLRTVSWSNFAALVLAPGVPLTHP
VPHWSVLRAREAGVEVIGDIELFCRERRRHAPDAPFIAITGTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPR
MGRVHVIEMSSYQIDLTPSLDPSIGILLNVSEDHIDRHGTIENYAAVKERLVAGVKPQGTAIVGVDDGFCRAIADRLDQA
GKRVVRISVKNPLADGLYTEHETIVRSSGGARSEVARLGGIGSLRGQHNAQNAACAAAAALAIGVREDVLQQGLRSFPGL
AHRMEQVGRLGNVLFVNDSKGTNADATAHALSSFADIFWIAGGKPKAGGITSLAGFFPRIRKAYLIGEAAKEFAGTLGEK
VPHAMSGTLEVAVEQAAADAKASGLKEAVVLLSPACASFDQFRNFEIRGTRFRDLVQALPGVEKVV

Sequences:

>Translated_466_residues
MIPVTSFAGKTVAVFGLGGSGLASCHALRAGGAEVIAADDGADRMTEAAKAGFITADLRTVSWSNFAALVLAPGVPLTHP
VPHWSVLRAREAGVEVIGDIELFCRERRRHAPDAPFIAITGTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPR
MGRVHVIEMSSYQIDLTPSLDPSIGILLNVSEDHIDRHGTIENYAAVKERLVAGVKPQGTAIVGVDDGFCRAIADRLDQA
GKRVVRISVKNPLADGLYTEHETIVRSSGGARSEVARLGGIGSLRGQHNAQNAACAAAAALAIGVREDVLQQGLRSFPGL
AHRMEQVGRLGNVLFVNDSKGTNADATAHALSSFADIFWIAGGKPKAGGITSLAGFFPRIRKAYLIGEAAKEFAGTLGEK
VPHAMSGTLEVAVEQAAADAKASGLKEAVVLLSPACASFDQFRNFEIRGTRFRDLVQALPGVEKVV
>Mature_466_residues
MIPVTSFAGKTVAVFGLGGSGLASCHALRAGGAEVIAADDGADRMTEAAKAGFITADLRTVSWSNFAALVLAPGVPLTHP
VPHWSVLRAREAGVEVIGDIELFCRERRRHAPDAPFIAITGTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPR
MGRVHVIEMSSYQIDLTPSLDPSIGILLNVSEDHIDRHGTIENYAAVKERLVAGVKPQGTAIVGVDDGFCRAIADRLDQA
GKRVVRISVKNPLADGLYTEHETIVRSSGGARSEVARLGGIGSLRGQHNAQNAACAAAAALAIGVREDVLQQGLRSFPGL
AHRMEQVGRLGNVLFVNDSKGTNADATAHALSSFADIFWIAGGKPKAGGITSLAGFFPRIRKAYLIGEAAKEFAGTLGEK
VPHAMSGTLEVAVEQAAADAKASGLKEAVVLLSPACASFDQFRNFEIRGTRFRDLVQALPGVEKVV

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=467, Percent_Identity=34.0471092077088, Blast_Score=191, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013116
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF07991 IlvN; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48905; Mature: 48905

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTSFAGKTVAVFGLGGSGLASCHALRAGGAEVIAADDGADRMTEAAKAGFITADLRT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEEEEE
VSWSNFAALVLAPGVPLTHPVPHWSVLRAREAGVEVIGDIELFCRERRRHAPDAPFIAIT
ECCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPRMGRVHVIEMSSYQIDLTPSL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSIGILLNVSEDHIDRHGTIENYAAVKERLVAGVKPQGTAIVGVDDGFCRAIADRLDQA
CCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHC
GKRVVRISVKNPLADGLYTEHETIVRSSGGARSEVARLGGIGSLRGQHNAQNAACAAAAA
CCEEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LAIGVREDVLQQGLRSFPGLAHRMEQVGRLGNVLFVNDSKGTNADATAHALSSFADIFWI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEE
AGGKPKAGGITSLAGFFPRIRKAYLIGEAAKEFAGTLGEKVPHAMSGTLEVAVEQAAADA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
KASGLKEAVVLLSPACASFDQFRNFEIRGTRFRDLVQALPGVEKVV
HHHHHHHHHHHHCCHHHHHHHHCCCEECCHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MIPVTSFAGKTVAVFGLGGSGLASCHALRAGGAEVIAADDGADRMTEAAKAGFITADLRT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEEEEE
VSWSNFAALVLAPGVPLTHPVPHWSVLRAREAGVEVIGDIELFCRERRRHAPDAPFIAIT
ECCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPRMGRVHVIEMSSYQIDLTPSL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSIGILLNVSEDHIDRHGTIENYAAVKERLVAGVKPQGTAIVGVDDGFCRAIADRLDQA
CCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHC
GKRVVRISVKNPLADGLYTEHETIVRSSGGARSEVARLGGIGSLRGQHNAQNAACAAAAA
CCEEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LAIGVREDVLQQGLRSFPGLAHRMEQVGRLGNVLFVNDSKGTNADATAHALSSFADIFWI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEE
AGGKPKAGGITSLAGFFPRIRKAYLIGEAAKEFAGTLGEKVPHAMSGTLEVAVEQAAADA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
KASGLKEAVVLLSPACASFDQFRNFEIRGTRFRDLVQALPGVEKVV
HHHHHHHHHHHHCCHHHHHHHHCCCEECCHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]