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The map label for this gene is ligA [H]

Identifier: 146342488

GI number: 146342488

Start: 5837705

End: 5839855

Strand: Reverse

Name: ligA [H]

Synonym: BRADO5649

Alternate gene names: 146342488

Gene position: 5839855-5837705 (Counterclockwise)

Preceding gene: 146342489

Following gene: 146342485

Centisome position: 78.32

GC content: 64.44

Gene sequence:

>2151_bases
ATGGCCAAAACAGCAAAATCCAAGCAGTCCGTCGACGTCGCCGATCTGACCAAGGCCCAGGCCAAGGTGGAGTGGAAGCG
GCTGGCGCTGGAACTCGAGACCCACGATAGGCTCTACTACCAGGAGGACGCGCCGAAGATTTCGGACGCCGCCTACGACG
AGCTGCGTCGTCGCTTCAACGCCATCGAAAAGCGCTTTCCCGAACTCGTCAGCCGCGACTCGCCGTCCCAGACGGTGGGC
GCCGCACCGTCCGGCCGGTTCAAGAAGGTCCGGCATGCGGTGCCGATGCTGTCGCTCGATAATGCCTTCGCCGAAGAGGA
CGTGCGCGACTTCGTCGGCCGCATCGCGCGCTTCCTGAAGCTTGCCGAGGATGATCGCATCGCTTTCTCCGCAGAGCCGA
AGATCGACGGGCTGTCGATGTCGCTGCGCTACGAGGGCGGCCAACTGGTCACAGCGGCGACCCGCGGCGACGGCGCGGAG
GGCGAGGACGTCACTGCCAACATCCGTACCTTGAAGGACGTGCCGCAGAAGCTGCATGGCCGCAACCTTCCCGACATCTG
CGAGATCCGCGGCGAGGTCTACATGACCAAGCAGGCGTTTCTCGCGCTCAACGAGCGCCAGAAGGAAGCCGGGGACACGA
TTTTCGCGAATCCCCGCAACTCCGCCGCGGGGTCGTTGCGCCAGAAGGACCCGACCATCACGGCGTCGCGGCCCCTCGGC
TTCTTCGCCTATGCCTGGGGCGACATGAGCGCGATGCCCGCGGAGACGCAGAGCGGCATGATCAAATGGTTCGAGCATTG
TGGCTTCACCACCAACCCGCTGACCAAGCTCTGCCACTCGGTCGAGGAGCTGATTGCGTTCCACCGCGCGATCGAGGAGC
AGCGCGCCGAGCTCGACTATGACATCGACGGTGTCGTCTACAAGGTCGACCGCATCGACTGGCAGGAGCGGCTCGGCTTC
GTCTCGCGCACGCCGCGCTGGGGCATCGCGCACAAATTCCCGGCCGAGCGTGCCATGACCGTGCTGAAGGACATCGAGAT
CCAGGTCGGCCGCACCGGCTCGTTCACGCCAGTCGGCAAGCTTGAGCCGGTCGGCGTCGGCGGCGTCATCGTGCAGAACG
TCACCCTGCACAATGAGGACTACATCAAGGGAATCGGCAACAAGGGCGAGGTCTTGCGGGAGGGGCGCGACATCCGTATC
GGCGACACCGTCGTCATCCAGCGCGCCGGCGATGTCATTCCTCAGGTGGTTGACGTTCTCATCGACAAGCGGCCTGCCGA
TGCCGAGGTGTTTGCCTTTCCCAGAAAGTGTCCCTGCCCGCTACACACCGACGTCGTCCGCGAGGAGACTGCGGCCGGCG
AGGAGGGCTCGCGCGCCCGCTGCACCGGCGAGTTCGCCTGTCCCTATCAGAAGATCGAGCACCTCAAGCTGTTCGCCTCG
CGCCGCGCCTTCGATATCGACGGTCTCGGCGAAAAGCAGATCCAGTTCTTCTTCGATGAAGGCTGGGTGAAGGAGCCGGC
CGACATCTTCACCCTGCAGAAGCGCAACGCCAAGCTGAAGCTGGAGGAGGTCGAGGGCTATGGCGAGACCTCCGTGCGCA
ATCTCTTCAACGCCATCGAGGCGCGGCGCGAGATGGCGCTGGAGCGGTTCATCTACGCGCTCGGCATGCGCTATGTCGGC
GAGACCACGGCCCTGGCGCTGGCGCGCGGCTACGGCTCCTGGGAGGCGTTCCACGACGCCTGCCTGAAGGTTGCCAATGG
CGACGAGGAGGCGATCGCCGAGATGGATGCGCTCGACCAGATCGGCGAAACAGTGATCAAGAGCGTCGCCGCCTATTTCG
GCGAAGACCACAATCGCGGCATCGTCGAGCGGCTGACCCGCGAGGTGAAGATCCTGGATGCCGAGAAGCCCAAGCGTAAT
TCGCCGATCGCGACCAAGACGGTGGTGTTCACGGGCACGCTGGAAAAGATGACGCGCGACGAGGCCAAGGCCACAGCCGA
GCGGCTCGGCGCGAAAGTGTCGGGATCGGTATCAAAGAAGACGGACTACGTCGTCGCCGGTCCTGGTGCGGGCTCGAAGC
TGAAGGATGCCGAGAAGCACGGCGTCAAGGTGCTGACCGAGGACGAGTGGCTGCAGCTGATCGGGGAGTAG

Upstream 100 bases:

>100_bases
TGACCCGAACGCTTTTGGCACCGCCGCGCGCATTGTGTAAACCGTCGTCCTTGGGAGGCCTGAAGCCTGCCCCTGGTTCG
ACGAGCAGACATTACGCAGT

Downstream 100 bases:

>100_bases
CTGTTCGTGGTGGCGGTCGCCGCCGATGCTGTTCGGCTTCGATCGCGCGCGCATGTTCTGCGCTGCGCCCCACGCGTGAG
GTGGACCGCATGATCTGCCA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MAKTAKSKQSVDVADLTKAQAKVEWKRLALELETHDRLYYQEDAPKISDAAYDELRRRFNAIEKRFPELVSRDSPSQTVG
AAPSGRFKKVRHAVPMLSLDNAFAEEDVRDFVGRIARFLKLAEDDRIAFSAEPKIDGLSMSLRYEGGQLVTAATRGDGAE
GEDVTANIRTLKDVPQKLHGRNLPDICEIRGEVYMTKQAFLALNERQKEAGDTIFANPRNSAAGSLRQKDPTITASRPLG
FFAYAWGDMSAMPAETQSGMIKWFEHCGFTTNPLTKLCHSVEELIAFHRAIEEQRAELDYDIDGVVYKVDRIDWQERLGF
VSRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGKLEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRI
GDTVVIQRAGDVIPQVVDVLIDKRPADAEVFAFPRKCPCPLHTDVVREETAAGEEGSRARCTGEFACPYQKIEHLKLFAS
RRAFDIDGLGEKQIQFFFDEGWVKEPADIFTLQKRNAKLKLEEVEGYGETSVRNLFNAIEARREMALERFIYALGMRYVG
ETTALALARGYGSWEAFHDACLKVANGDEEAIAEMDALDQIGETVIKSVAAYFGEDHNRGIVERLTREVKILDAEKPKRN
SPIATKTVVFTGTLEKMTRDEAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAEKHGVKVLTEDEWLQLIGE

Sequences:

>Translated_716_residues
MAKTAKSKQSVDVADLTKAQAKVEWKRLALELETHDRLYYQEDAPKISDAAYDELRRRFNAIEKRFPELVSRDSPSQTVG
AAPSGRFKKVRHAVPMLSLDNAFAEEDVRDFVGRIARFLKLAEDDRIAFSAEPKIDGLSMSLRYEGGQLVTAATRGDGAE
GEDVTANIRTLKDVPQKLHGRNLPDICEIRGEVYMTKQAFLALNERQKEAGDTIFANPRNSAAGSLRQKDPTITASRPLG
FFAYAWGDMSAMPAETQSGMIKWFEHCGFTTNPLTKLCHSVEELIAFHRAIEEQRAELDYDIDGVVYKVDRIDWQERLGF
VSRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGKLEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRI
GDTVVIQRAGDVIPQVVDVLIDKRPADAEVFAFPRKCPCPLHTDVVREETAAGEEGSRARCTGEFACPYQKIEHLKLFAS
RRAFDIDGLGEKQIQFFFDEGWVKEPADIFTLQKRNAKLKLEEVEGYGETSVRNLFNAIEARREMALERFIYALGMRYVG
ETTALALARGYGSWEAFHDACLKVANGDEEAIAEMDALDQIGETVIKSVAAYFGEDHNRGIVERLTREVKILDAEKPKRN
SPIATKTVVFTGTLEKMTRDEAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAEKHGVKVLTEDEWLQLIGE
>Mature_715_residues
AKTAKSKQSVDVADLTKAQAKVEWKRLALELETHDRLYYQEDAPKISDAAYDELRRRFNAIEKRFPELVSRDSPSQTVGA
APSGRFKKVRHAVPMLSLDNAFAEEDVRDFVGRIARFLKLAEDDRIAFSAEPKIDGLSMSLRYEGGQLVTAATRGDGAEG
EDVTANIRTLKDVPQKLHGRNLPDICEIRGEVYMTKQAFLALNERQKEAGDTIFANPRNSAAGSLRQKDPTITASRPLGF
FAYAWGDMSAMPAETQSGMIKWFEHCGFTTNPLTKLCHSVEELIAFHRAIEEQRAELDYDIDGVVYKVDRIDWQERLGFV
SRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGKLEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRIG
DTVVIQRAGDVIPQVVDVLIDKRPADAEVFAFPRKCPCPLHTDVVREETAAGEEGSRARCTGEFACPYQKIEHLKLFASR
RAFDIDGLGEKQIQFFFDEGWVKEPADIFTLQKRNAKLKLEEVEGYGETSVRNLFNAIEARREMALERFIYALGMRYVGE
TTALALARGYGSWEAFHDACLKVANGDEEAIAEMDALDQIGETVIKSVAAYFGEDHNRGIVERLTREVKILDAEKPKRNS
PIATKTVVFTGTLEKMTRDEAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAEKHGVKVLTEDEWLQLIGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=692, Percent_Identity=46.6763005780347, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI87082305, Length=545, Percent_Identity=24.5871559633028, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 79735; Mature: 79604

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTAKSKQSVDVADLTKAQAKVEWKRLALELETHDRLYYQEDAPKISDAAYDELRRRFN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCHHHHHHHHHHHHH
AIEKRFPELVSRDSPSQTVGAAPSGRFKKVRHAVPMLSLDNAFAEEDVRDFVGRIARFLK
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAEDDRIAFSAEPKIDGLSMSLRYEGGQLVTAATRGDGAEGEDVTANIRTLKDVPQKLHG
HCCCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
RNLPDICEIRGEVYMTKQAFLALNERQKEAGDTIFANPRNSAAGSLRQKDPTITASRPLG
CCCCHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEECCCCCC
FFAYAWGDMSAMPAETQSGMIKWFEHCGFTTNPLTKLCHSVEELIAFHRAIEEQRAELDY
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIDGVVYKVDRIDWQERLGFVSRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGK
CCCCEEEEEECCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCC
LEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRIGDTVVIQRAGDVIPQVVDVL
CCCCCCCCEEEEEEEECCCHHHHCCCCCCHHHHCCCCEEECCEEEEECHHHHHHHHHHHH
IDKRPADAEVFAFPRKCPCPLHTDVVREETAAGEEGSRARCTGEFACPYQKIEHLKLFAS
HCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHH
RRAFDIDGLGEKQIQFFFDEGWVKEPADIFTLQKRNAKLKLEEVEGYGETSVRNLFNAIE
CCCCCCCCCCCHHEEEEECCCCCCCCHHHEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
ARREMALERFIYALGMRYVGETTALALARGYGSWEAFHDACLKVANGDEEAIAEMDALDQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
IGETVIKSVAAYFGEDHNRGIVERLTREVKILDAEKPKRNSPIATKTVVFTGTLEKMTRD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCHHHHHHH
EAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAEKHGVKVLTEDEWLQLIGE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCEEEEHHHHHHHHCC
>Mature Secondary Structure 
AKTAKSKQSVDVADLTKAQAKVEWKRLALELETHDRLYYQEDAPKISDAAYDELRRRFN
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCHHHHHHHHHHHHH
AIEKRFPELVSRDSPSQTVGAAPSGRFKKVRHAVPMLSLDNAFAEEDVRDFVGRIARFLK
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAEDDRIAFSAEPKIDGLSMSLRYEGGQLVTAATRGDGAEGEDVTANIRTLKDVPQKLHG
HCCCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
RNLPDICEIRGEVYMTKQAFLALNERQKEAGDTIFANPRNSAAGSLRQKDPTITASRPLG
CCCCHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEECCCCCC
FFAYAWGDMSAMPAETQSGMIKWFEHCGFTTNPLTKLCHSVEELIAFHRAIEEQRAELDY
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIDGVVYKVDRIDWQERLGFVSRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGK
CCCCEEEEEECCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCC
LEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRIGDTVVIQRAGDVIPQVVDVL
CCCCCCCCEEEEEEEECCCHHHHCCCCCCHHHHCCCCEEECCEEEEECHHHHHHHHHHHH
IDKRPADAEVFAFPRKCPCPLHTDVVREETAAGEEGSRARCTGEFACPYQKIEHLKLFAS
HCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHH
RRAFDIDGLGEKQIQFFFDEGWVKEPADIFTLQKRNAKLKLEEVEGYGETSVRNLFNAIE
CCCCCCCCCCCHHEEEEECCCCCCCCHHHEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
ARREMALERFIYALGMRYVGETTALALARGYGSWEAFHDACLKVANGDEEAIAEMDALDQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
IGETVIKSVAAYFGEDHNRGIVERLTREVKILDAEKPKRNSPIATKTVVFTGTLEKMTRD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCHHHHHHH
EAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAEKHGVKVLTEDEWLQLIGE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA