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The map label for this gene is algI [H]

Identifier: 146342479

GI number: 146342479

Start: 5825322

End: 5826704

Strand: Reverse

Name: algI [H]

Synonym: BRADO5639

Alternate gene names: 146342479

Gene position: 5826704-5825322 (Counterclockwise)

Preceding gene: 146342480

Following gene: 146342478

Centisome position: 78.14

GC content: 65.37

Gene sequence:

>1383_bases
ATGCTGTTCAATGGCTATCCGTTCCTGCTGGTGTTTCTGCCTGTCGCCATCGTGCTGGCGCGGCTTGCCGATCCCTTCCC
GTCGTTGCGGATGCCCGTGCTGGCAGGACTGTCGCTCGCCTTCTACGGCTATGGCGATCCGCCGTTCCTGCTGCTGCTGA
TCGCCTCCATCCTCGGCAATTGGCTCGCCGCGCGACTGTTCGCCCGCTTCAAGGCCCGCGGCATCCTGACGTTTGCGATC
CTCGCCAATTTCGTCGTCCTCGGCCTGTTCAAATACGCCGACTTCGCCGCTGCCAATCTCGGACTGCTGATCGGCCATCA
TTTCCCGCCGCTGCAGTTGGCGCTGCCGCTCGGCATCTCGTTCTTCACCTTCCACCACATCATGTATCTGGCCGATCTGC
GCGCGGGGAAGGCGCCGTTGACGTCGCTCGACCGCTATGCGCTGTACATCGCCTTTTTCCCGCAGGCGATCGCCGGGCCG
CTGGCGCGCTGGTCGGAGGTGATGGGGCAGTTCGGGCAACGGATCTATGCGCCGGGATGGCAGCAGCGGTTCTATCTCGG
CATCGTCTTCATCGTCGGGGGCTTGATCGAGAAGATCATGTTCGGCGACCGGCTCGGCCGCCTGCTCGATCCGATCTATG
CGCAGGCGGCGAGCGGCCCGTTGCCGGCCGGCGAGGCGTGGCTTGCAGCGTTCGCGTCGGTGCAGATCCTCTACGACTTC
GCCGGCTACTCCGACATCGCCATCGGCCTCGGTTTGCTGTTCGGCGTGCGGTTGCCGTTCAATTTCGACGCGCCGCTGCG
TGCCACTAACATGCAGGATTTCTGGCAGCGCTGGCACATGACGCTGATGGCGTTCCTGCGCGACTACGTGTTCCGGCCGA
TGGTCAACGCCCGGCTGCTGCCGCGGCGGCTGTTCGCGGTGCAGTATTTCGCGGCCATGTTCATCACCATGATCCTGTGC
GGCCTGTGGCATGGCGCCAGCTGGAGCTTCGTGTTGTGGGGCACGCTGCACGGCATGGCCGTCGTCATCTGCGCGCTGTG
GCGTCGGTTCGGTCCGCGACTGCCGGCGGGGCTCGGCTGGCTGCTGACCTTTCTCGTCGTGCTGGGCACCGGCGTCATCT
TCCGCGCCGGCTCGGTGGAAGCGGCGTGGCACGTGTTCCAGGGACTGGCCTCACCGCCGCCGTTCGATCGCGGCGTGCGG
CTGCTCCCGGTCGTGATCCCGACCCTGGCCGCGCTGCTGCTACCGGCCAGCCAGGACGTGGCCGCCCGGCTGACGCGCCG
TCCGAATGTCGGCTTGGCTTGTCTGCTCGGCGTAGCGGTGCTGGCGCTGTTGATCGAGCTCGGAGAGCGTAATGTCCATG
AGTTCATCTATTTCAAGTTCTGA

Upstream 100 bases:

>100_bases
GATCACCGCGCGCATGACGGCGCACCCGGCCGAAGCCGCGCCCGATTTCGGGCATGGCTGATTGTCTTGGGATTGAGCAG
GGCGGGCGGGACGCCGCCTG

Downstream 100 bases:

>100_bases
GGCCGTCGCGCCGGCGGCCTGGGGCCGCTGCATCGCCGCGTTCCTTGCTGCGCTTGGCGGTGGCGCCTTGCTCGTGCTGG
CGCTGATGATCATGGTCGAT

Product: putative membrane bound O-acyl transferase (MBOAT) family protein alginate O-acetyltransferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLFNGYPFLLVFLPVAIVLARLADPFPSLRMPVLAGLSLAFYGYGDPPFLLLLIASILGNWLAARLFARFKARGILTFAI
LANFVVLGLFKYADFAAANLGLLIGHHFPPLQLALPLGISFFTFHHIMYLADLRAGKAPLTSLDRYALYIAFFPQAIAGP
LARWSEVMGQFGQRIYAPGWQQRFYLGIVFIVGGLIEKIMFGDRLGRLLDPIYAQAASGPLPAGEAWLAAFASVQILYDF
AGYSDIAIGLGLLFGVRLPFNFDAPLRATNMQDFWQRWHMTLMAFLRDYVFRPMVNARLLPRRLFAVQYFAAMFITMILC
GLWHGASWSFVLWGTLHGMAVVICALWRRFGPRLPAGLGWLLTFLVVLGTGVIFRAGSVEAAWHVFQGLASPPPFDRGVR
LLPVVIPTLAALLLPASQDVAARLTRRPNVGLACLLGVAVLALLIELGERNVHEFIYFKF

Sequences:

>Translated_460_residues
MLFNGYPFLLVFLPVAIVLARLADPFPSLRMPVLAGLSLAFYGYGDPPFLLLLIASILGNWLAARLFARFKARGILTFAI
LANFVVLGLFKYADFAAANLGLLIGHHFPPLQLALPLGISFFTFHHIMYLADLRAGKAPLTSLDRYALYIAFFPQAIAGP
LARWSEVMGQFGQRIYAPGWQQRFYLGIVFIVGGLIEKIMFGDRLGRLLDPIYAQAASGPLPAGEAWLAAFASVQILYDF
AGYSDIAIGLGLLFGVRLPFNFDAPLRATNMQDFWQRWHMTLMAFLRDYVFRPMVNARLLPRRLFAVQYFAAMFITMILC
GLWHGASWSFVLWGTLHGMAVVICALWRRFGPRLPAGLGWLLTFLVVLGTGVIFRAGSVEAAWHVFQGLASPPPFDRGVR
LLPVVIPTLAALLLPASQDVAARLTRRPNVGLACLLGVAVLALLIELGERNVHEFIYFKF
>Mature_460_residues
MLFNGYPFLLVFLPVAIVLARLADPFPSLRMPVLAGLSLAFYGYGDPPFLLLLIASILGNWLAARLFARFKARGILTFAI
LANFVVLGLFKYADFAAANLGLLIGHHFPPLQLALPLGISFFTFHHIMYLADLRAGKAPLTSLDRYALYIAFFPQAIAGP
LARWSEVMGQFGQRIYAPGWQQRFYLGIVFIVGGLIEKIMFGDRLGRLLDPIYAQAASGPLPAGEAWLAAFASVQILYDF
AGYSDIAIGLGLLFGVRLPFNFDAPLRATNMQDFWQRWHMTLMAFLRDYVFRPMVNARLLPRRLFAVQYFAAMFITMILC
GLWHGASWSFVLWGTLHGMAVVICALWRRFGPRLPAGLGWLLTFLVVLGTGVIFRAGSVEAAWHVFQGLASPPPFDRGVR
LLPVVIPTLAALLLPASQDVAARLTRRPNVGLACLLGVAVLALLIELGERNVHEFIYFKF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 51055; Mature: 51055

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECC
>Mature Secondary Structure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HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]