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The map label for this gene is fadD25 [H]

Identifier: 146342477

GI number: 146342477

Start: 5822606

End: 5824324

Strand: Direct

Name: fadD25 [H]

Synonym: BRADO5637

Alternate gene names: 146342477

Gene position: 5822606-5824324 (Clockwise)

Preceding gene: 146342476

Following gene: 146342482

Centisome position: 78.09

GC content: 68.59

Gene sequence:

>1719_bases
ATGGATCAGTTCGGCTCGCTGGTGGCCCTGCTCGCGGCGCGAGCGCAATCGCAGGCCGACGATCCCGCTTATCTGTTCCT
CAACGATCGCGGCACCGAGGAATCGAGGCTGACATTCGGCGACCTGCAGGCGAGAGCCCGCGACATGGCGGCGCGGCTCG
CCGACCACGCCGCGCCCGGCGACCGCGCGCTTCTGGTGTTTCCGCCGGGGCTCGAATTCTTTGTTGCCTTTTTCGGCTGC
CTGATCGCGGGCATCATCGCCGTTCCCATGATGATGCCGCGGCGGCTGGGAGCGCGTGATGCGAGCGGCGCGATCATCGC
CGACTGCGCGCCCAGGCTCGCGCTGAGCAGCTCCGCCTTTGCGCTGCGTGGCGATCTCAGAGAGCGCTTTGCGCGCGACG
GGCTGACGTGGCTGGAGGTCGATCTGACGTCATCCGACGCCGGCGGCACCGCGCCGGCACGCCGCATCGAGGCCGACGAC
GTCGCCTTCCTGCAATACACGTCCGGATCGACATCGGAGCCGAAGGGTGTCGCGGTGAGCCACGCCAACCTGCTCGCCAA
TTCGGAGATGATCCGCATCGCGCTCGGCAACAGCCGGAGCTCGACCTACGTCAACTGGGTGCCGCTCTATCACGACATGG
GGCTGATCCTGAACGCGCTCCAGACGCTGTATGTCGGATCGCTCTGCGTGCTGATGGCGCCCAATGCGTTCACGCAGCGC
CCGCTGAACTGGCTGCGCGCGATCGGCCGCTACCGCGCCGAGGTCGCCTGCTGTCCGAACTTCGGCTACGACCTCTGCGC
CGCGCGCTATCGCGCCGACCAGATGCAGGATGTCGATCTGTCCTGCTGGAAGGTGGCCCTCAACGGCGCCGAGCCGGTGC
GCGCGGACACGCTGCACCGGTTCACGCAGACCTTTGCGCCGCATGGCTTCGCGGCAGAGGCGATCTACCCCGCCTATGGC
ATGGCGGAGGCCACCCTGCTGATCTCGGGCGGCCGTCGCGGCGCCGGGCCATTGCTCCGCGAGCTCAGCCATCGCGCGTT
GCAGACGCATCGCGCGGAGGCTCCGTCGACCCCGGAGGATTCGCAGGTGGTCGTCGGCTGCGGCCGCGCGCTTGCCGGCG
AGCAGATCGCCATCGTCGATCCCGAGAGCTGCAGGCGGCTGGCCTGCGACGAGGTCGGCGAGATCTGGGTGGCCGGCCCG
AACGTTGCCCGCTCCTATTGGCGCAATCCGGAAGCGACCGTCGCGAGCCTGCGCGCGACCATCACAGGCGACGCTAGCGG
GGTGTCCTGGCTGCGCACCGGCGATCTCGGCTTTCTCGATGCCGATGGCGAGCTGTTCATCACCGGCCGCATCAAGGATC
TCCTGATCATCCGGGGCGTCAACCATTACCCGCAGGACATCGAGCACACGGTGCAGGCCACCGATCCGGCGCTGCGGCCG
AACTGCGGAGCGGCGTTCTCGGTTCCGGACGAGGATGGCAGCGAGACGCTCGCCATCGTGCAGGAGGTCGAGCGCACCGA
GCGCAAGCGGATCGATCTCGCCGAGCTCACGAGCATGATCCGGGAGCGCGTCACCGAGCAGCACGAGGTATTCGCGCGCC
ACATCGCGCTGATCCGCCCCGGGCAGCTGCCGAAGACGACCAGCGGCAAGATCCAGCGCCGACGGGCGCGGCAGATGTGG
CTCGACGGACAGTTGCCGCCGCTCGACGGCGCCCAATGA

Upstream 100 bases:

>100_bases
GACTTGTACGACTATCCGACGATCGACGAGTTGTCGCAGCAGCTGGCAGGTCGTTGCGCGGCACGGTCTGCCGCGTAGGC
TCAAGCTCAACACGCGCCCA

Downstream 100 bases:

>100_bases
CGGATCGCGTCAGAACTCGATCCGCGCCGCCGCTGCATCGCGGAAGCTTGCGATGATGCCGCCATTGACGCGTCGCGCGA
TCGGCGCGCGATAGTGGATG

Product: putative fatty-acid--CoA ligase (Acyl-CoA synthetase)

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MDQFGSLVALLAARAQSQADDPAYLFLNDRGTEESRLTFGDLQARARDMAARLADHAAPGDRALLVFPPGLEFFVAFFGC
LIAGIIAVPMMMPRRLGARDASGAIIADCAPRLALSSSAFALRGDLRERFARDGLTWLEVDLTSSDAGGTAPARRIEADD
VAFLQYTSGSTSEPKGVAVSHANLLANSEMIRIALGNSRSSTYVNWVPLYHDMGLILNALQTLYVGSLCVLMAPNAFTQR
PLNWLRAIGRYRAEVACCPNFGYDLCAARYRADQMQDVDLSCWKVALNGAEPVRADTLHRFTQTFAPHGFAAEAIYPAYG
MAEATLLISGGRRGAGPLLRELSHRALQTHRAEAPSTPEDSQVVVGCGRALAGEQIAIVDPESCRRLACDEVGEIWVAGP
NVARSYWRNPEATVASLRATITGDASGVSWLRTGDLGFLDADGELFITGRIKDLLIIRGVNHYPQDIEHTVQATDPALRP
NCGAAFSVPDEDGSETLAIVQEVERTERKRIDLAELTSMIRERVTEQHEVFARHIALIRPGQLPKTTSGKIQRRRARQMW
LDGQLPPLDGAQ

Sequences:

>Translated_572_residues
MDQFGSLVALLAARAQSQADDPAYLFLNDRGTEESRLTFGDLQARARDMAARLADHAAPGDRALLVFPPGLEFFVAFFGC
LIAGIIAVPMMMPRRLGARDASGAIIADCAPRLALSSSAFALRGDLRERFARDGLTWLEVDLTSSDAGGTAPARRIEADD
VAFLQYTSGSTSEPKGVAVSHANLLANSEMIRIALGNSRSSTYVNWVPLYHDMGLILNALQTLYVGSLCVLMAPNAFTQR
PLNWLRAIGRYRAEVACCPNFGYDLCAARYRADQMQDVDLSCWKVALNGAEPVRADTLHRFTQTFAPHGFAAEAIYPAYG
MAEATLLISGGRRGAGPLLRELSHRALQTHRAEAPSTPEDSQVVVGCGRALAGEQIAIVDPESCRRLACDEVGEIWVAGP
NVARSYWRNPEATVASLRATITGDASGVSWLRTGDLGFLDADGELFITGRIKDLLIIRGVNHYPQDIEHTVQATDPALRP
NCGAAFSVPDEDGSETLAIVQEVERTERKRIDLAELTSMIRERVTEQHEVFARHIALIRPGQLPKTTSGKIQRRRARQMW
LDGQLPPLDGAQ
>Mature_572_residues
MDQFGSLVALLAARAQSQADDPAYLFLNDRGTEESRLTFGDLQARARDMAARLADHAAPGDRALLVFPPGLEFFVAFFGC
LIAGIIAVPMMMPRRLGARDASGAIIADCAPRLALSSSAFALRGDLRERFARDGLTWLEVDLTSSDAGGTAPARRIEADD
VAFLQYTSGSTSEPKGVAVSHANLLANSEMIRIALGNSRSSTYVNWVPLYHDMGLILNALQTLYVGSLCVLMAPNAFTQR
PLNWLRAIGRYRAEVACCPNFGYDLCAARYRADQMQDVDLSCWKVALNGAEPVRADTLHRFTQTFAPHGFAAEAIYPAYG
MAEATLLISGGRRGAGPLLRELSHRALQTHRAEAPSTPEDSQVVVGCGRALAGEQIAIVDPESCRRLACDEVGEIWVAGP
NVARSYWRNPEATVASLRATITGDASGVSWLRTGDLGFLDADGELFITGRIKDLLIIRGVNHYPQDIEHTVQATDPALRP
NCGAAFSVPDEDGSETLAIVQEVERTERKRIDLAELTSMIRERVTEQHEVFARHIALIRPGQLPKTTSGKIQRRRARQMW
LDGQLPPLDGAQ

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=604, Percent_Identity=27.4834437086093, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI45827692, Length=604, Percent_Identity=27.4834437086093, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI44888818, Length=607, Percent_Identity=26.3591433278418, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI55749758, Length=601, Percent_Identity=26.4559068219634, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI225735625, Length=604, Percent_Identity=26.6556291390728, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI45827694, Length=509, Percent_Identity=27.3084479371316, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI225735627, Length=509, Percent_Identity=27.3084479371316, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI45827696, Length=509, Percent_Identity=27.3084479371316, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI45827698, Length=472, Percent_Identity=27.3305084745763, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1788107, Length=599, Percent_Identity=25.3756260434057, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=25, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789201, Length=426, Percent_Identity=23.7089201877934, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193202679, Length=617, Percent_Identity=24.9594813614263, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=558, Percent_Identity=23.4767025089606, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=564, Percent_Identity=24.290780141844, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=579, Percent_Identity=24.1796200345423, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=591, Percent_Identity=23.1810490693739, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=419, Percent_Identity=24.1050119331742, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=416, Percent_Identity=22.3557692307692, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557194, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24654656, Length=450, Percent_Identity=24.4444444444444, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859661, Length=414, Percent_Identity=25.3623188405797, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355181, Length=479, Percent_Identity=24.8434237995825, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581924, Length=399, Percent_Identity=27.5689223057644, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922652, Length=423, Percent_Identity=25.7683215130024, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648260, Length=191, Percent_Identity=31.413612565445, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=347, Percent_Identity=24.207492795389, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281365686, Length=404, Percent_Identity=23.2673267326733, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076582, Length=412, Percent_Identity=23.5436893203883, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62296; Mature: 62296

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFGSLVALLAARAQSQADDPAYLFLNDRGTEESRLTFGDLQARARDMAARLADHAAPG
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DRALLVFPPGLEFFVAFFGCLIAGIIAVPMMMPRRLGARDASGAIIADCAPRLALSSSAF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHCCCCH
ALRGDLRERFARDGLTWLEVDLTSSDAGGTAPARRIEADDVAFLQYTSGSTSEPKGVAVS
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCEEEE
HANLLANSEMIRIALGNSRSSTYVNWVPLYHDMGLILNALQTLYVGSLCVLMAPNAFTQR
HHHHHCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
PLNWLRAIGRYRAEVACCPNFGYDLCAARYRADQMQDVDLSCWKVALNGAEPVRADTLHR
HHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHH
FTQTFAPHGFAAEAIYPAYGMAEATLLISGGRRGAGPLLRELSHRALQTHRAEAPSTPED
HHHHHCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SQVVVGCGRALAGEQIAIVDPESCRRLACDEVGEIWVAGPNVARSYWRNPEATVASLRAT
CEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCHHHHHHEEE
ITGDASGVSWLRTGDLGFLDADGELFITGRIKDLLIIRGVNHYPQDIEHTVQATDPALRP
EECCCCCCCEEEECCCEEEECCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
NCGAAFSVPDEDGSETLAIVQEVERTERKRIDLAELTSMIRERVTEQHEVFARHIALIRP
CCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQLPKTTSGKIQRRRARQMWLDGQLPPLDGAQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDQFGSLVALLAARAQSQADDPAYLFLNDRGTEESRLTFGDLQARARDMAARLADHAAPG
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DRALLVFPPGLEFFVAFFGCLIAGIIAVPMMMPRRLGARDASGAIIADCAPRLALSSSAF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHCCCCH
ALRGDLRERFARDGLTWLEVDLTSSDAGGTAPARRIEADDVAFLQYTSGSTSEPKGVAVS
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCEEEE
HANLLANSEMIRIALGNSRSSTYVNWVPLYHDMGLILNALQTLYVGSLCVLMAPNAFTQR
HHHHHCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
PLNWLRAIGRYRAEVACCPNFGYDLCAARYRADQMQDVDLSCWKVALNGAEPVRADTLHR
HHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHH
FTQTFAPHGFAAEAIYPAYGMAEATLLISGGRRGAGPLLRELSHRALQTHRAEAPSTPED
HHHHHCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SQVVVGCGRALAGEQIAIVDPESCRRLACDEVGEIWVAGPNVARSYWRNPEATVASLRAT
CEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCHHHHHHEEE
ITGDASGVSWLRTGDLGFLDADGELFITGRIKDLLIIRGVNHYPQDIEHTVQATDPALRP
EECCCCCCCEEEECCCEEEECCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
NCGAAFSVPDEDGSETLAIVQEVERTERKRIDLAELTSMIRERVTEQHEVFARHIALIRP
CCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQLPKTTSGKIQRRRARQMWLDGQLPPLDGAQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]