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The map label for this gene is dfrA [H]

Identifier: 146342463

GI number: 146342463

Start: 5810816

End: 5811841

Strand: Direct

Name: dfrA [H]

Synonym: BRADO5622

Alternate gene names: 146342463

Gene position: 5810816-5811841 (Clockwise)

Preceding gene: 146342462

Following gene: 146342464

Centisome position: 77.93

GC content: 66.86

Gene sequence:

>1026_bases
ATGAAAGTCCTCGTTACCGGCGGCAGCGGCTTCATCGGCCATCATCTCGTCTCTGCGCTCGTGGCGCGGGGGATGGACGT
GCGCGTGCTCGACATCCGCTCGCCGACCCATATGATGGCCGAGGCGGAGTATCTTGAGGGCTCGGTGCTGGATGCCGACC
TGGTGAAGCGGGCGGTCGCCGGGGTCGATCAGGTCTACCATCTCGCCGGGCTGCCCGGCATGTGGATGCCGGACCGCGAG
GATTTCTACCGCGTGAACTGCGTCGGCACCGAAACCGTGCTGGCCGCGGCCCGCGCCGGTAAGGTGCGCCGCTTCCTGCA
TTGTTCCACCGAGTCGATTCTGTTCGACTACCCCGGCTCGACCAATGCAGCCGCGGCCCCCACGGCACCGCCGGCGGACG
CGATGCCGGGCGCCTACACCCGATCCAAAGCGCTGGCCGAGGCCCGCGCCATGGCAGCAGCGGCGGACGGTTTCCCGGTC
GTCGTCGGCACCCCAACGATGCCGATCGGGCCGCACGACAGCAACCTGACACCGCCTTCGCAGATGCTGCGGCACTTCCT
CGACAGCCGGGTGCAGCTCTACCTCGATTTCATCGTCAATCTGGTCGATGTTCGCGACGTCGCGGCGGGTCTGATCCTGG
CGATGGAGCGCGGCAAGGTCGGCGATCGTTACGTGTTCGGTGGCGAGAGCCTGCGTCTCAGCCGCATCCTCGAGCTGATG
GCGACGATCAGCGGCCGCAAGCATGTGGCGATCTCCGTGCCTGGCGGTTTCGCCGAACTGTCGGCCGGCATGTTGGAGCT
GATCTCCGACCGCCTCACCAAGCAGTGTCCGTCCGGCACGGCCGAAGGCGTGCGGATCGCCCGTTCGGCCTCTGACCTGT
CGATCGACAAGGCCAAGCGGGAGCTCGGCTACACGCCCCGTCCGATCGAGCCGACCTTGCGCGAGACCATCGACTTCCTG
CTCGGCACCGAGCAGGCCAAGCGGCTCGACGAGAAACAACACCCTTTTCGCACTGCGGCCGAATAG

Upstream 100 bases:

>100_bases
TGGGATTCAAGCCGTCGGTGGCTGATCTCATCACGCCGGACTGGTCGCCGGATCGGCTTGCATCGGACCGTCTTCAAGGC
GATCAGCACTCGCGTCCCTA

Downstream 100 bases:

>100_bases
CTAAGAGCAGCCGGTCCCACGTCCCATAACGGTTTTCAAAGACATGTCCGGCGATGCCAGCCCCATCTCGTCAGTTGCGT
TCAGCGGCTGGACCACCACC

Product: putative NAD dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVLVTGGSGFIGHHLVSALVARGMDVRVLDIRSPTHMMAEAEYLEGSVLDADLVKRAVAGVDQVYHLAGLPGMWMPDRE
DFYRVNCVGTETVLAAARAGKVRRFLHCSTESILFDYPGSTNAAAAPTAPPADAMPGAYTRSKALAEARAMAAAADGFPV
VVGTPTMPIGPHDSNLTPPSQMLRHFLDSRVQLYLDFIVNLVDVRDVAAGLILAMERGKVGDRYVFGGESLRLSRILELM
ATISGRKHVAISVPGGFAELSAGMLELISDRLTKQCPSGTAEGVRIARSASDLSIDKAKRELGYTPRPIEPTLRETIDFL
LGTEQAKRLDEKQHPFRTAAE

Sequences:

>Translated_341_residues
MKVLVTGGSGFIGHHLVSALVARGMDVRVLDIRSPTHMMAEAEYLEGSVLDADLVKRAVAGVDQVYHLAGLPGMWMPDRE
DFYRVNCVGTETVLAAARAGKVRRFLHCSTESILFDYPGSTNAAAAPTAPPADAMPGAYTRSKALAEARAMAAAADGFPV
VVGTPTMPIGPHDSNLTPPSQMLRHFLDSRVQLYLDFIVNLVDVRDVAAGLILAMERGKVGDRYVFGGESLRLSRILELM
ATISGRKHVAISVPGGFAELSAGMLELISDRLTKQCPSGTAEGVRIARSASDLSIDKAKRELGYTPRPIEPTLRETIDFL
LGTEQAKRLDEKQHPFRTAAE
>Mature_341_residues
MKVLVTGGSGFIGHHLVSALVARGMDVRVLDIRSPTHMMAEAEYLEGSVLDADLVKRAVAGVDQVYHLAGLPGMWMPDRE
DFYRVNCVGTETVLAAARAGKVRRFLHCSTESILFDYPGSTNAAAAPTAPPADAMPGAYTRSKALAEARAMAAAADGFPV
VVGTPTMPIGPHDSNLTPPSQMLRHFLDSRVQLYLDFIVNLVDVRDVAAGLILAMERGKVGDRYVFGGESLRLSRILELM
ATISGRKHVAISVPGGFAELSAGMLELISDRLTKQCPSGTAEGVRIARSASDLSIDKAKRELGYTPRPIEPTLRETIDFL
LGTEQAKRLDEKQHPFRTAAE

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=323, Percent_Identity=28.4829721362229, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8393516, Length=323, Percent_Identity=28.4829721362229, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239745448, Length=331, Percent_Identity=27.7945619335347, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310132178, Length=331, Percent_Identity=27.4924471299094, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310113012, Length=331, Percent_Identity=27.4924471299094, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116268111, Length=345, Percent_Identity=25.5072463768116, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786974, Length=253, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081792, Length=325, Percent_Identity=24.6153846153846, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17570557, Length=333, Percent_Identity=22.5225225225225, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71987463, Length=242, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36753; Mature: 36753

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGGSGFIGHHLVSALVARGMDVRVLDIRSPTHMMAEAEYLEGSVLDADLVKRAVA
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHH
GVDQVYHLAGLPGMWMPDREDFYRVNCVGTETVLAAARAGKVRRFLHCSTESILFDYPGS
HHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
TNAAAAPTAPPADAMPGAYTRSKALAEARAMAAAADGFPVVVGTPTMPIGPHDSNLTPPS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
QMLRHFLDSRVQLYLDFIVNLVDVRDVAAGLILAMERGKVGDRYVFGGESLRLSRILELM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCCCCCEEEECCCCHHHHHHHHHH
ATISGRKHVAISVPGGFAELSAGMLELISDRLTKQCPSGTAEGVRIARSASDLSIDKAKR
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
ELGYTPRPIEPTLRETIDFLLGTEQAKRLDEKQHPFRTAAE
HCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKVLVTGGSGFIGHHLVSALVARGMDVRVLDIRSPTHMMAEAEYLEGSVLDADLVKRAVA
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHH
GVDQVYHLAGLPGMWMPDREDFYRVNCVGTETVLAAARAGKVRRFLHCSTESILFDYPGS
HHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
TNAAAAPTAPPADAMPGAYTRSKALAEARAMAAAADGFPVVVGTPTMPIGPHDSNLTPPS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
QMLRHFLDSRVQLYLDFIVNLVDVRDVAAGLILAMERGKVGDRYVFGGESLRLSRILELM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCCCCCEEEECCCCHHHHHHHHHH
ATISGRKHVAISVPGGFAELSAGMLELISDRLTKQCPSGTAEGVRIARSASDLSIDKAKR
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
ELGYTPRPIEPTLRETIDFLLGTEQAKRLDEKQHPFRTAAE
HCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]