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The map label for this gene is thyA [H]

Identifier: 146342419

GI number: 146342419

Start: 5761728

End: 5762522

Strand: Reverse

Name: thyA [H]

Synonym: BRADO5578

Alternate gene names: 146342419

Gene position: 5762522-5761728 (Counterclockwise)

Preceding gene: 146342420

Following gene: 146342418

Centisome position: 77.28

GC content: 63.14

Gene sequence:

>795_bases
ATGCACCAGTATCACGACCTGCTCGAACGCATTTTGTCCGATGGCGCGCAGAAGCACGACCGCACCGGCACCGGCACGCT
GTCCGTGTTCGGCCATCAGATGCGCTTCAACCTCGCCGCCGGCTTCCCGATGGTCACCACCAAGCGGTTGCCGCTGAAGG
CGATCGTGCACGAGCTGCTGTGGTTTCTGAAGGGCGACACCAACATCAAATATCTGCACGACCACGGAGTCACCATCTGG
GACGAGTGGGCCGATGCCAACGGCGATCTCGGTCCCGTCTACGGCTACCAGTGGCGCTCCTGGCCGACGTCGGACGGCGG
CCAGATCGACCAGATCAGCAATGTCGTCGACATGATCCGGCGCAACCCGGATTCGCGCCGGCTGATCGTCACCGCGTGGA
ATCCGGCCGACGTCGAGAAGATGGCGCTGCCGCCGTGCCACTGCCTGTTCCAGTTCTACGTGGCCAATGGCAAGCTGTCC
TGCCAGCTCTATCAGCGCTCGGCCGACGTCTTCCTCGGCGTGCCCTTCAACATCGCCTCCTATGCGCTGCTGACGATGAT
GGTGGCGCAAGTGACGGGGCTGAAGCTCGGCGAGTTCGTGCACTCGTTCGGCGACGTGCATCTCTATTCCAACCACATCG
AGCAGGCCCGCCTGCAACTGAGCCGAACCCCGCGGCCGCTGCCGACCATGACGCTCAATCCCGACGTGAAGGACATCTTC
GCCTTCCGCTATGAGGATTTTGCGCTCGCCGGCTACGATCCGCATCCGCACATCAAGGCCGAGGTCGCGGTCTAG

Upstream 100 bases:

>100_bases
AGCATATTTCTATGGCGAAGGGGGCTCTGGCGGCCGGGACTGTCGGCGGCAAAGCGGGTCCGCTAAACCTGCACCTTCAC
AAACCCAGACGTCGCCGGCC

Downstream 100 bases:

>100_bases
CGCATGCCGGTCACGATCCGCAGCGCCAAGCCTTCCGACATCGGCGTCGTGTTCGCGCTGATCCGCGAACTCGCCGATTA
CGAGAAGCTCTCGCAAGAGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHQYHDLLERILSDGAQKHDRTGTGTLSVFGHQMRFNLAAGFPMVTTKRLPLKAIVHELLWFLKGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGYQWRSWPTSDGGQIDQISNVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGKLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKLGEFVHSFGDVHLYSNHIEQARLQLSRTPRPLPTMTLNPDVKDIF
AFRYEDFALAGYDPHPHIKAEVAV

Sequences:

>Translated_264_residues
MHQYHDLLERILSDGAQKHDRTGTGTLSVFGHQMRFNLAAGFPMVTTKRLPLKAIVHELLWFLKGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGYQWRSWPTSDGGQIDQISNVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGKLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKLGEFVHSFGDVHLYSNHIEQARLQLSRTPRPLPTMTLNPDVKDIF
AFRYEDFALAGYDPHPHIKAEVAV
>Mature_264_residues
MHQYHDLLERILSDGAQKHDRTGTGTLSVFGHQMRFNLAAGFPMVTTKRLPLKAIVHELLWFLKGDTNIKYLHDHGVTIW
DEWADANGDLGPVYGYQWRSWPTSDGGQIDQISNVVDMIRRNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGKLS
CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKLGEFVHSFGDVHLYSNHIEQARLQLSRTPRPLPTMTLNPDVKDIF
AFRYEDFALAGYDPHPHIKAEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=53.1914893617021, Blast_Score=303, Evalue=8e-83,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.9090909090909, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71993377, Length=284, Percent_Identity=46.1267605633803, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=41.4383561643836, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17137556, Length=286, Percent_Identity=55.2447552447552, Blast_Score=312, Evalue=2e-85,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 29965; Mature: 29965

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQYHDLLERILSDGAQKHDRTGTGTLSVFGHQMRFNLAAGFPMVTTKRLPLKAIVHELL
CCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEHEEEEEEECCCCEEECCCCCHHHHHHHHH
WFLKGDTNIKYLHDHGVTIWDEWADANGDLGPVYGYQWRSWPTSDGGQIDQISNVVDMIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLKLGEFVHSFGDVHLYSNHIEQARLQLSRTPRPLPTMTLNPDVKDIF
HHHHHHHHHHHHCCHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
AFRYEDFALAGYDPHPHIKAEVAV
HHHCCCEEEECCCCCCCCEEEECC
>Mature Secondary Structure
MHQYHDLLERILSDGAQKHDRTGTGTLSVFGHQMRFNLAAGFPMVTTKRLPLKAIVHELL
CCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEHEEEEEEECCCCEEECCCCCHHHHHHHHH
WFLKGDTNIKYLHDHGVTIWDEWADANGDLGPVYGYQWRSWPTSDGGQIDQISNVVDMIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVEKMALPPCHCLFQFYVANGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLKLGEFVHSFGDVHLYSNHIEQARLQLSRTPRPLPTMTLNPDVKDIF
HHHHHHHHHHHHCCHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
AFRYEDFALAGYDPHPHIKAEVAV
HHHCCCEEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA