Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 146342384

GI number: 146342384

Start: 5723469

End: 5724716

Strand: Direct

Name: bcr [H]

Synonym: BRADO5541

Alternate gene names: 146342384

Gene position: 5723469-5724716 (Clockwise)

Preceding gene: 146342381

Following gene: 146342386

Centisome position: 76.76

GC content: 68.75

Gene sequence:

>1248_bases
TTGACCGACACCAGTGCCGACAACATCGAGCTCGTCCGTCACCGCCCGATGGCGTTCGCGGAGTTCGTCATCGTGATCGC
GGCGATCATGGCGCTGAACCCGCTCGCCATGGACATGATGCTGCCGGCGCTGCCGGACATCGGCTCGGCGTTCCACATCG
GCACCGCCAACCGGGTGCAGATGGTGCTGTCGGCGTTCGTGATCGGCTTCGGCGTCGGCCAGTTCGCGATCGGCCCGCTG
TCGGACAGTTTCGGCCGCCGTCCCGTGCTGCTCAGCGGCATGGTGCTGTACGTCATCGCCAGCCTGCTCGCGCTCGCGGC
GCCGTCATTCGAGACGCTGCTGCTGGCGCGCGTGCTGGAAGGCCTCGGCACTGCCGCGACCCGCGTGATCGCGACGTCCA
TCGTGCGCGACTGCTATGCCGGCCGGCGCATGGCCAGCGTGGTGTCGCTGGCGATGATGGTGTTCATCGCGGTCCCCGTG
GTGGCGCCGGCCTTCGGCCAGGCGGTGATGCTGCTGACGCAATGGCGCGGCATCTTCGTGGTGCTGACCATCTATGGCGT
GATCGCGCTGCTGTGGACGGTGTTCCGCCTGCCGGAGACGCTGCCGGAGACCGAGCGCAAGCCATTCAACGTCGCCGCGA
TCAGCGCCGCCTTCCGCGCTACGCTCACCAATCGCCAGACGCTGGGCTATGCGCTCGCCGCCAGCGGCATTTTCGGCGCG
GTGCTGGCCTATGTGCTGTCGTCGCAGCAGCTGTTCACCGGCGTCTATCATCTCGGCCACTACTTCCCGCTGGCGTTTGC
CGCGATCTCGGCCACCATCGCCGTCGCCGGCTTCCTCAATGCGCGGCTGGTCGGCCGGCTCGGCATGCGCAAGATCTCGC
ACAGCGCGCTGGTCGGCTGCGTGATGGTCGCACTCGCCTGCTTCGCCGCGGCCCTGCTCGGCGCGCTGCCGCTGTGGCTG
TTCATGGTGCTGTCGGCGGCGATGATGTTCTCGTTCGGCCTGATGTTCGCGAACTTCACCGCGCTGGCGATGGAGCCGCA
GCGCGCCAATGCCGGCACTGCGTCGTCGCTCTACGGCTCGATCACCACGCTGCTCGGCATGGCCGCCGGCGCCGTGGTCG
GCCAGGCCTTCGACGGCACGCCGCTGCCGTTCGCGACCGGCTTCCTCGCCTGCACCGCGACCGCGCTGGCCATCGTGCTG
ATCACCGAGAAGGGCCGTCTGTTCCACGGCGCCGCCAAGCCGGTCTGA

Upstream 100 bases:

>100_bases
CTGACCAGCCATTCCGAAACGCGTCTTGTGCGACGCAGCAAGTCCGATACACATCAAAGCTCGCAACCGCCGTCCCCCCG
CCCTTACGGAAAGCCGCGCC

Downstream 100 bases:

>100_bases
ACCGGCCCTAGAGGCAGGACCCCTGCCCGCGCTGCGGGCACATCTTCAGCACGCTCGACAGCGACAGCAGCAGACGCGCG
CTGCGCGTCGAATTGTCGGG

Product: Bcr/CflA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTDTSADNIELVRHRPMAFAEFVIVIAAIMALNPLAMDMMLPALPDIGSAFHIGTANRVQMVLSAFVIGFGVGQFAIGPL
SDSFGRRPVLLSGMVLYVIASLLALAAPSFETLLLARVLEGLGTAATRVIATSIVRDCYAGRRMASVVSLAMMVFIAVPV
VAPAFGQAVMLLTQWRGIFVVLTIYGVIALLWTVFRLPETLPETERKPFNVAAISAAFRATLTNRQTLGYALAASGIFGA
VLAYVLSSQQLFTGVYHLGHYFPLAFAAISATIAVAGFLNARLVGRLGMRKISHSALVGCVMVALACFAAALLGALPLWL
FMVLSAAMMFSFGLMFANFTALAMEPQRANAGTASSLYGSITTLLGMAAGAVVGQAFDGTPLPFATGFLACTATALAIVL
ITEKGRLFHGAAKPV

Sequences:

>Translated_415_residues
MTDTSADNIELVRHRPMAFAEFVIVIAAIMALNPLAMDMMLPALPDIGSAFHIGTANRVQMVLSAFVIGFGVGQFAIGPL
SDSFGRRPVLLSGMVLYVIASLLALAAPSFETLLLARVLEGLGTAATRVIATSIVRDCYAGRRMASVVSLAMMVFIAVPV
VAPAFGQAVMLLTQWRGIFVVLTIYGVIALLWTVFRLPETLPETERKPFNVAAISAAFRATLTNRQTLGYALAASGIFGA
VLAYVLSSQQLFTGVYHLGHYFPLAFAAISATIAVAGFLNARLVGRLGMRKISHSALVGCVMVALACFAAALLGALPLWL
FMVLSAAMMFSFGLMFANFTALAMEPQRANAGTASSLYGSITTLLGMAAGAVVGQAFDGTPLPFATGFLACTATALAIVL
ITEKGRLFHGAAKPV
>Mature_414_residues
TDTSADNIELVRHRPMAFAEFVIVIAAIMALNPLAMDMMLPALPDIGSAFHIGTANRVQMVLSAFVIGFGVGQFAIGPLS
DSFGRRPVLLSGMVLYVIASLLALAAPSFETLLLARVLEGLGTAATRVIATSIVRDCYAGRRMASVVSLAMMVFIAVPVV
APAFGQAVMLLTQWRGIFVVLTIYGVIALLWTVFRLPETLPETERKPFNVAAISAAFRATLTNRQTLGYALAASGIFGAV
LAYVLSSQQLFTGVYHLGHYFPLAFAAISATIAVAGFLNARLVGRLGMRKISHSALVGCVMVALACFAAALLGALPLWLF
MVLSAAMMFSFGLMFANFTALAMEPQRANAGTASSLYGSITTLLGMAAGAVVGQAFDGTPLPFATGFLACTATALAIVLI
TEKGRLFHGAAKPV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=389, Percent_Identity=29.0488431876607, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082312, Length=341, Percent_Identity=26.3929618768328, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790146, Length=183, Percent_Identity=32.2404371584699, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994889, Length=180, Percent_Identity=32.7777777777778, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790794, Length=360, Percent_Identity=22.5, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787065, Length=336, Percent_Identity=24.1071428571429, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081983, Length=171, Percent_Identity=25.7309941520468, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367379, Length=282, Percent_Identity=25.531914893617, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=165, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43640; Mature: 43508

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSADNIELVRHRPMAFAEFVIVIAAIMALNPLAMDMMLPALPDIGSAFHIGTANRVQ
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHH
MVLSAFVIGFGVGQFAIGPLSDSFGRRPVLLSGMVLYVIASLLALAAPSFETLLLARVLE
HHHHHHHHHHCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLGTAATRVIATSIVRDCYAGRRMASVVSLAMMVFIAVPVVAPAFGQAVMLLTQWRGIFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLTIYGVIALLWTVFRLPETLPETERKPFNVAAISAAFRATLTNRQTLGYALAASGIFGA
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAYVLSSQQLFTGVYHLGHYFPLAFAAISATIAVAGFLNARLVGRLGMRKISHSALVGC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVALACFAAALLGALPLWLFMVLSAAMMFSFGLMFANFTALAMEPQRANAGTASSLYGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
ITTLLGMAAGAVVGQAFDGTPLPFATGFLACTATALAIVLITEKGRLFHGAAKPV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEECCCCCCC
>Mature Secondary Structure 
TDTSADNIELVRHRPMAFAEFVIVIAAIMALNPLAMDMMLPALPDIGSAFHIGTANRVQ
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHH
MVLSAFVIGFGVGQFAIGPLSDSFGRRPVLLSGMVLYVIASLLALAAPSFETLLLARVLE
HHHHHHHHHHCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLGTAATRVIATSIVRDCYAGRRMASVVSLAMMVFIAVPVVAPAFGQAVMLLTQWRGIFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLTIYGVIALLWTVFRLPETLPETERKPFNVAAISAAFRATLTNRQTLGYALAASGIFGA
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAYVLSSQQLFTGVYHLGHYFPLAFAAISATIAVAGFLNARLVGRLGMRKISHSALVGC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVALACFAAALLGALPLWLFMVLSAAMMFSFGLMFANFTALAMEPQRANAGTASSLYGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
ITTLLGMAAGAVVGQAFDGTPLPFATGFLACTATALAIVLITEKGRLFHGAAKPV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]