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The map label for this gene is leuA

Identifier: 146342371

GI number: 146342371

Start: 5712612

End: 5714189

Strand: Reverse

Name: leuA

Synonym: BRADO5528

Alternate gene names: 146342371

Gene position: 5714189-5712612 (Counterclockwise)

Preceding gene: 146342372

Following gene: 146342370

Centisome position: 76.63

GC content: 65.59

Gene sequence:

>1578_bases
ATGTCCAATCCCGTTCAGTCCGACAAGGACCGCGTCGTCATTTTCGACACCACCCTGCGCGACGGCGAGCAGTGCCCCGG
CGCCACCATGACGTTCGAGGAGAAGCTCAACATCGCCGCCATGCTGGACGACATGGGCGTCGACATCATCGAGGCCGGCT
TCCCGATTGCCTCTGACGGCGACTTCGAGGCCGTCAACGAGATCGCCAAGCGGTCGAAGAACGCGGTGATCTGCGGCCTG
TCGCGCGCCGGCGCCAAGGACATCGATCGCTGCGCCGAGGCGATCCGCCCGGCCAAGCGCGGCCGCATCCACACCTTCCT
GTCCACCTCGCCGGTGCATATGAAGTACAAGCTGCAGATGGATCCGCAGCAGGTGTTCGAACTGGTGATCTCCTCGGTGA
CCCGCGCGCGCAACCACACCGACGACGTCGAGTGGTCGTCGGAGGACGGCACCCGCACCGAGTTCGACTTCCTGTGCCGC
TGCGTCGAGGCCGCGATCAAGGCCGGCGCCAGCACCATCAACATCCCGGACACCGTCGGCTACGCCGTGCCGGAGGAGTA
TTACGACCTGTTCAAGCGGGTGCGCGAGACGGTGCCGAACTCCGACAAGGCCGTGTTCTCCGTCCACTGCCACAACGATC
TCGGCATGGCGGTCGCCAACTCGATGGCCGGCGTGCGCGCCGGCGCCCGCCAGATCGAATGCACAATCAACGGCATCGGC
GAGCGCGCCGGCAACGCGGCGCTCGAAGAGGTCGTGATGGCGATGCGCGTGCGCAACGACAAGCTGCCGTTCTGGAACAA
GATCGACACCACGCAGCTGACTCATGCCTCGAAGGTGGTGTCGGCGGCGACCTCGTTCCCGGTGCAGTACAACAAGGCCA
TCGTCGGCCGCAACGCCTTCGCGCATGAGAGCGGCATCCATCAGGACGGCATGCTGAAGAACGCGCAGACCTACGAGATC
ATGCTGCCAGAGACGGTCGGCGTGAAGCAGACCTCGCTGGTGATGGGCAAGCATTCCGGCCGTCACGCCTTCATCCACAA
GCTGGAGGAGATGGGCCACAAGCTGTCGAGCAACCAGGTCGAGGATGCCTTCGTCCGCTTCAAGGCGCTGGCCGATCGCA
AGAAGCAGATCTACGACGAGGACATCGAGGCCCTGATCGACGAGGGCATGGTGAGCGCCCATGACCGCATCAAGCTGCTG
TCTTTGAGCGTGATCGCCGGCACCCGCGGCCCGCAGCGCGCGACCATGAAGCTCGACATCGACGGCGTGACCAGGATCGA
GGAGAGCGAGGGCAACGGTCCGGTCGATGCGGTGTTCAACTGCATCAAGTCGCTGGTGCCGCACGAGGCCAAGCTGGAGC
TGTACCAGGTCCATGCCGTCACCGAGGGCACCGACGCGCAGGCCGAAGTCTCGGTGCGGCTGTCGCAGGACGGCCGCTCG
ATGACCGCGCGCGCCGCAGATCCGGACACGCTGGTCGCCTCGGCCAAGGCCTATCTCGGAGCGCTGAACAAGCTGGTCAT
GAAGCGCCAGCGCGATGTCGCGCCAGGGCATGGCGCCTCGGCGGCCGCGGCGAGCTGA

Upstream 100 bases:

>100_bases
CACTCAGGGGGCTGCTTCGACACCGAAACACCCTTCAGCGCCCAATGTCTGGAGATCCTGACAGCCAGGCGCACCACCTT
CAGACTCCGAGGAATAGCCA

Downstream 100 bases:

>100_bases
TCCCGCGCAACGTCATTCCGGGGCAGCCGCGCCAGCGGCTGAACCCGGAATCCCGAGCTTGGTTTGCACCTCTTCCTACA
ATTTCGAGGTTTCCCAATGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSNPVQSDKDRVVIFDTTLRDGEQCPGATMTFEEKLNIAAMLDDMGVDIIEAGFPIASDGDFEAVNEIAKRSKNAVICGL
SRAGAKDIDRCAEAIRPAKRGRIHTFLSTSPVHMKYKLQMDPQQVFELVISSVTRARNHTDDVEWSSEDGTRTEFDFLCR
CVEAAIKAGASTINIPDTVGYAVPEEYYDLFKRVRETVPNSDKAVFSVHCHNDLGMAVANSMAGVRAGARQIECTINGIG
ERAGNAALEEVVMAMRVRNDKLPFWNKIDTTQLTHASKVVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKNAQTYEI
MLPETVGVKQTSLVMGKHSGRHAFIHKLEEMGHKLSSNQVEDAFVRFKALADRKKQIYDEDIEALIDEGMVSAHDRIKLL
SLSVIAGTRGPQRATMKLDIDGVTRIEESEGNGPVDAVFNCIKSLVPHEAKLELYQVHAVTEGTDAQAEVSVRLSQDGRS
MTARAADPDTLVASAKAYLGALNKLVMKRQRDVAPGHGASAAAAS

Sequences:

>Translated_525_residues
MSNPVQSDKDRVVIFDTTLRDGEQCPGATMTFEEKLNIAAMLDDMGVDIIEAGFPIASDGDFEAVNEIAKRSKNAVICGL
SRAGAKDIDRCAEAIRPAKRGRIHTFLSTSPVHMKYKLQMDPQQVFELVISSVTRARNHTDDVEWSSEDGTRTEFDFLCR
CVEAAIKAGASTINIPDTVGYAVPEEYYDLFKRVRETVPNSDKAVFSVHCHNDLGMAVANSMAGVRAGARQIECTINGIG
ERAGNAALEEVVMAMRVRNDKLPFWNKIDTTQLTHASKVVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKNAQTYEI
MLPETVGVKQTSLVMGKHSGRHAFIHKLEEMGHKLSSNQVEDAFVRFKALADRKKQIYDEDIEALIDEGMVSAHDRIKLL
SLSVIAGTRGPQRATMKLDIDGVTRIEESEGNGPVDAVFNCIKSLVPHEAKLELYQVHAVTEGTDAQAEVSVRLSQDGRS
MTARAADPDTLVASAKAYLGALNKLVMKRQRDVAPGHGASAAAAS
>Mature_524_residues
SNPVQSDKDRVVIFDTTLRDGEQCPGATMTFEEKLNIAAMLDDMGVDIIEAGFPIASDGDFEAVNEIAKRSKNAVICGLS
RAGAKDIDRCAEAIRPAKRGRIHTFLSTSPVHMKYKLQMDPQQVFELVISSVTRARNHTDDVEWSSEDGTRTEFDFLCRC
VEAAIKAGASTINIPDTVGYAVPEEYYDLFKRVRETVPNSDKAVFSVHCHNDLGMAVANSMAGVRAGARQIECTINGIGE
RAGNAALEEVVMAMRVRNDKLPFWNKIDTTQLTHASKVVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKNAQTYEIM
LPETVGVKQTSLVMGKHSGRHAFIHKLEEMGHKLSSNQVEDAFVRFKALADRKKQIYDEDIEALIDEGMVSAHDRIKLLS
LSVIAGTRGPQRATMKLDIDGVTRIEESEGNGPVDAVFNCIKSLVPHEAKLELYQVHAVTEGTDAQAEVSVRLSQDGRSM
TARAADPDTLVASAKAYLGALNKLVMKRQRDVAPGHGASAAAAS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=46.7741935483871, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320019, Length=375, Percent_Identity=28.2666666666667, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=377, Percent_Identity=28.1167108753316, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=540, Percent_Identity=25.7407407407407, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=502, Percent_Identity=26.0956175298805, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BRASO (A4YZ76)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001207419.1
- ProteinModelPortal:   A4YZ76
- STRING:   A4YZ76
- GeneID:   5119952
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO5528
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   BSP376:BRADO5528-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57275; Mature: 57143

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPVQSDKDRVVIFDTTLRDGEQCPGATMTFEEKLNIAAMLDDMGVDIIEAGFPIASDG
CCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHCCHHHHHHHCCCHHHHCCCCCCCCC
DFEAVNEIAKRSKNAVICGLSRAGAKDIDRCAEAIRPAKRGRIHTFLSTSPVHMKYKLQM
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCHHHCCCEEEEECCCCEEEEEEECC
DPQQVFELVISSVTRARNHTDDVEWSSEDGTRTEFDFLCRCVEAAIKAGASTINIPDTVG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCC
YAVPEEYYDLFKRVRETVPNSDKAVFSVHCHNDLGMAVANSMAGVRAGARQIECTINGIG
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCC
ERAGNAALEEVVMAMRVRNDKLPFWNKIDTTQLTHASKVVSAATSFPVQYNKAIVGRNAF
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCEEECCCCH
AHESGIHQDGMLKNAQTYEIMLPETVGVKQTSLVMGKHSGRHAFIHKLEEMGHKLSSNQV
HHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCHH
EDAFVRFKALADRKKQIYDEDIEALIDEGMVSAHDRIKLLSLSVIAGTRGPQRATMKLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEC
DGVTRIEESEGNGPVDAVFNCIKSLVPHEAKLELYQVHAVTEGTDAQAEVSVRLSQDGRS
CCCEEEECCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCEEEEEEECCCCCE
MTARAADPDTLVASAKAYLGALNKLVMKRQRDVAPGHGASAAAAS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNPVQSDKDRVVIFDTTLRDGEQCPGATMTFEEKLNIAAMLDDMGVDIIEAGFPIASDG
CCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHCCHHHHHHHCCCHHHHCCCCCCCCC
DFEAVNEIAKRSKNAVICGLSRAGAKDIDRCAEAIRPAKRGRIHTFLSTSPVHMKYKLQM
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCHHHCCCEEEEECCCCEEEEEEECC
DPQQVFELVISSVTRARNHTDDVEWSSEDGTRTEFDFLCRCVEAAIKAGASTINIPDTVG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCC
YAVPEEYYDLFKRVRETVPNSDKAVFSVHCHNDLGMAVANSMAGVRAGARQIECTINGIG
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCC
ERAGNAALEEVVMAMRVRNDKLPFWNKIDTTQLTHASKVVSAATSFPVQYNKAIVGRNAF
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCEEECCCCH
AHESGIHQDGMLKNAQTYEIMLPETVGVKQTSLVMGKHSGRHAFIHKLEEMGHKLSSNQV
HHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCHH
EDAFVRFKALADRKKQIYDEDIEALIDEGMVSAHDRIKLLSLSVIAGTRGPQRATMKLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEC
DGVTRIEESEGNGPVDAVFNCIKSLVPHEAKLELYQVHAVTEGTDAQAEVSVRLSQDGRS
CCCEEEECCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCEEEEEEECCCCCE
MTARAADPDTLVASAKAYLGALNKLVMKRQRDVAPGHGASAAAAS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA