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The map label for this gene is dapE [H]

Identifier: 146342364

GI number: 146342364

Start: 5703012

End: 5704403

Strand: Reverse

Name: dapE [H]

Synonym: BRADO5521

Alternate gene names: 146342364

Gene position: 5704403-5703012 (Counterclockwise)

Preceding gene: 146342366

Following gene: 146342362

Centisome position: 76.5

GC content: 63.94

Gene sequence:

>1392_bases
ATGAACGACATCAGCAGCCTGCCGTCTCCATCCGCCGGCCTGGATGCGGTGTTCGCCCATGTCGATAGCAACAAGCAGGA
TTTCCTTTCCCGCGTGATGGACTATGTGCGTCATCCCTCGATCAGCGCGCACAATGTCGGCATCACCGAGGTGGCCGCGC
TGCTGGTGAAGATGCTGCGGGAGATCGGGCTGGAAGCCGACACCGTGCCGACCGCCGGACATCCGATGGTGCTGGGACGC
TGGCACAAGAAGCCCGATGCGCCGACTGTGCTGCTGTATGGCCACTACGACGTGCAGCCGCCGGATCCGCTCGATCTGTG
GCTGAGCCCGCCCTTCGAGCCCACCATCCGCGACGGCCGCATCTATGCGCGCGGCATCGGCGACAACAAGGGCCAGCATT
TCGCGCAACTGATGGCGATCGAATCTCATTTGAAGGTGCACGGCAAGCTGCCCTGCAATGTCACGTTTCTGCTCGAGGGC
GAGGAGGAGATCGGCAGTCCGCGCATTGCCGAATTCGTGGCTGAACATCGCGAGCAGCTGAAGGCGGATGTCGTCGTCAC
CGCCGATGGGCCGATGCATCCGTCGGGGCGCCCGACAATGACCTATGGCGTTCGCGGCATGTGCTCGTTCGAGCTGCGCG
CCAGCCACGCGAATCGGGACGTGCATTCCGGCAATCTCGGCGGCATCGTGCCGAATCCGATCTGGACCCTGGTGCATCTG
CTCGGGACGATGAAGAACGCCGCCGGAGAGATCACGATTGAGGGTTTGCATGATGCCATCGTGCCGCCAAGCGCGCTCGA
TGTGGCTGCGGTGAAGCGCCTGCCGCTCGATCTCGACGCCGCCAAGGCGAGCCTCGGCCTTAGCCGGCTCGATGCGCCGG
CCGAACGCGGCTACTACGAAAGGCTGATGTTCCATCCGACGCTGACCATCAACGGCTTCCATGGCGGCTATGGCGGACCG
GGCAGCAAGACTGTCCTTCCCTGCGAGGCGTTCGTGAAGTGCGACATACGCCTGGTCGAGGCAATGACGCCGGACCACGT
GCTGGCCAAGGTCGAGGCCCATGTGAAAAAGCATGCGCCGGAGGTCGAGTTCATCCGGCGCGGCGGCATGCTGCCGTCGC
GCGTGCCGCTCGATTCGCCGTTTGCCGCGATCATCCGCGACGCGATCACGCTGGCGCAGGGCGTTGCGCCGCTCGAATTT
CCGTGTACCGGCGGCAGCTTGCCGGACTACGTCTTCACCAAAATTCTCGGTGTGCCGGCTTTCGTCGTCCCCTATGCCAA
CGCCGACGAGGCGAACCACGCGCCTAACGAAAACATGATCATCGACTGCTTCACCGACGGCATCCGCACCGGCGCGGCGC
TGCTTGACCGCGTCGGTGCAAGTCGCAATTAG

Upstream 100 bases:

>100_bases
GATGACGCGCGGAGTTCGCTTCGCTCGTCATTTGCGTTCCGGACGAATCCGATCGAACATGGCGACTCTCTCATCGCACG
GGCCAGGAGAAGCCAGCCGC

Downstream 100 bases:

>100_bases
TGCCGACGACGGGCGATCACGATTTTTTGCGTCGCCGAGGCGGTCATCGCGCGGCCACGAAACGATGGCATTGAGGCCGC
GCTTGCTTGCAGCGCGGGAT

Product: M20/M25/M40 family peptidase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNDISSLPSPSAGLDAVFAHVDSNKQDFLSRVMDYVRHPSISAHNVGITEVAALLVKMLREIGLEADTVPTAGHPMVLGR
WHKKPDAPTVLLYGHYDVQPPDPLDLWLSPPFEPTIRDGRIYARGIGDNKGQHFAQLMAIESHLKVHGKLPCNVTFLLEG
EEEIGSPRIAEFVAEHREQLKADVVVTADGPMHPSGRPTMTYGVRGMCSFELRASHANRDVHSGNLGGIVPNPIWTLVHL
LGTMKNAAGEITIEGLHDAIVPPSALDVAAVKRLPLDLDAAKASLGLSRLDAPAERGYYERLMFHPTLTINGFHGGYGGP
GSKTVLPCEAFVKCDIRLVEAMTPDHVLAKVEAHVKKHAPEVEFIRRGGMLPSRVPLDSPFAAIIRDAITLAQGVAPLEF
PCTGGSLPDYVFTKILGVPAFVVPYANADEANHAPNENMIIDCFTDGIRTGAALLDRVGASRN

Sequences:

>Translated_463_residues
MNDISSLPSPSAGLDAVFAHVDSNKQDFLSRVMDYVRHPSISAHNVGITEVAALLVKMLREIGLEADTVPTAGHPMVLGR
WHKKPDAPTVLLYGHYDVQPPDPLDLWLSPPFEPTIRDGRIYARGIGDNKGQHFAQLMAIESHLKVHGKLPCNVTFLLEG
EEEIGSPRIAEFVAEHREQLKADVVVTADGPMHPSGRPTMTYGVRGMCSFELRASHANRDVHSGNLGGIVPNPIWTLVHL
LGTMKNAAGEITIEGLHDAIVPPSALDVAAVKRLPLDLDAAKASLGLSRLDAPAERGYYERLMFHPTLTINGFHGGYGGP
GSKTVLPCEAFVKCDIRLVEAMTPDHVLAKVEAHVKKHAPEVEFIRRGGMLPSRVPLDSPFAAIIRDAITLAQGVAPLEF
PCTGGSLPDYVFTKILGVPAFVVPYANADEANHAPNENMIIDCFTDGIRTGAALLDRVGASRN
>Mature_463_residues
MNDISSLPSPSAGLDAVFAHVDSNKQDFLSRVMDYVRHPSISAHNVGITEVAALLVKMLREIGLEADTVPTAGHPMVLGR
WHKKPDAPTVLLYGHYDVQPPDPLDLWLSPPFEPTIRDGRIYARGIGDNKGQHFAQLMAIESHLKVHGKLPCNVTFLLEG
EEEIGSPRIAEFVAEHREQLKADVVVTADGPMHPSGRPTMTYGVRGMCSFELRASHANRDVHSGNLGGIVPNPIWTLVHL
LGTMKNAAGEITIEGLHDAIVPPSALDVAAVKRLPLDLDAAKASLGLSRLDAPAERGYYERLMFHPTLTINGFHGGYGGP
GSKTVLPCEAFVKCDIRLVEAMTPDHVLAKVEAHVKKHAPEVEFIRRGGMLPSRVPLDSPFAAIIRDAITLAQGVAPLEF
PCTGGSLPDYVFTKILGVPAFVVPYANADEANHAPNENMIIDCFTDGIRTGAALLDRVGASRN

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=476, Percent_Identity=29.4117647058824, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI21071039, Length=489, Percent_Identity=28.6298568507157, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI271398277, Length=325, Percent_Identity=28, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1788816, Length=327, Percent_Identity=26.2996941896024, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17563146, Length=453, Percent_Identity=30.6843267108168, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI193211059, Length=472, Percent_Identity=27.3305084745763, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321159, Length=480, Percent_Identity=27.5, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6319758, Length=477, Percent_Identity=22.2222222222222, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24585880, Length=482, Percent_Identity=28.6307053941909, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50025; Mature: 50025

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDISSLPSPSAGLDAVFAHVDSNKQDFLSRVMDYVRHPSISAHNVGITEVAALLVKMLR
CCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EIGLEADTVPTAGHPMVLGRWHKKPDAPTVLLYGHYDVQPPDPLDLWLSPPFEPTIRDGR
HHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
IYARGIGDNKGQHFAQLMAIESHLKVHGKLPCNVTFLLEGEEEIGSPRIAEFVAEHREQL
EEEEECCCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHH
KADVVVTADGPMHPSGRPTMTYGVRGMCSFELRASHANRDVHSGNLGGIVPNPIWTLVHL
CCCEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LGTMKNAAGEITIEGLHDAIVPPSALDVAAVKRLPLDLDAAKASLGLSRLDAPAERGYYE
HHHHHCCCCEEEEECCHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCHHHCCCCHHCCHHH
RLMFHPTLTINGFHGGYGGPGSKTVLPCEAFVKCDIRLVEAMTPDHVLAKVEAHVKKHAP
HHEECCEEEEECCCCCCCCCCCCEEECCHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCC
EVEFIRRGGMLPSRVPLDSPFAAIIRDAITLAQGVAPLEFPCTGGSLPDYVFTKILGVPA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCE
FVVPYANADEANHAPNENMIIDCFTDGIRTGAALLDRVGASRN
EEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDISSLPSPSAGLDAVFAHVDSNKQDFLSRVMDYVRHPSISAHNVGITEVAALLVKMLR
CCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EIGLEADTVPTAGHPMVLGRWHKKPDAPTVLLYGHYDVQPPDPLDLWLSPPFEPTIRDGR
HHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
IYARGIGDNKGQHFAQLMAIESHLKVHGKLPCNVTFLLEGEEEIGSPRIAEFVAEHREQL
EEEEECCCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHH
KADVVVTADGPMHPSGRPTMTYGVRGMCSFELRASHANRDVHSGNLGGIVPNPIWTLVHL
CCCEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LGTMKNAAGEITIEGLHDAIVPPSALDVAAVKRLPLDLDAAKASLGLSRLDAPAERGYYE
HHHHHCCCCEEEEECCHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCHHHCCCCHHCCHHH
RLMFHPTLTINGFHGGYGGPGSKTVLPCEAFVKCDIRLVEAMTPDHVLAKVEAHVKKHAP
HHEECCEEEEECCCCCCCCCCCCEEECCHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCC
EVEFIRRGGMLPSRVPLDSPFAAIIRDAITLAQGVAPLEFPCTGGSLPDYVFTKILGVPA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCE
FVVPYANADEANHAPNENMIIDCFTDGIRTGAALLDRVGASRN
EEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA