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The map label for this gene is sseA [H]

Identifier: 146342275

GI number: 146342275

Start: 5617806

End: 5618657

Strand: Direct

Name: sseA [H]

Synonym: BRADO5429

Alternate gene names: 146342275

Gene position: 5617806-5618657 (Clockwise)

Preceding gene: 146342274

Following gene: 146342276

Centisome position: 75.34

GC content: 67.84

Gene sequence:

>852_bases
ATGAGCAGAGATCCTTCCGACGCCCTCGTCAGCTCCGAATGGCTGGCGCAGCATTTGTCTGATCCGGATATCAAGATCCT
CGACTGCACCTGGCATCATCCGAGCACCAATCTCGACGGCCGCACACAGTATCGCGGCCGGCACCTGCCGGGCTCGGTGC
ATTTCGACATCGACCAGGTCGCCGACAAGACCAACCCGCTGCCGCATATGCTGCCGACGGCGGAGGACTTCGCCCACAAG
GTCGGGCTGCTCGGCATCAGCAATGCCGACCGGGTCATCGTCTATGACCGCCACTATGGCGGCTCCGCCGCGGGCCGCGT
GTGGTGGATGTTCCGCGTCTTCGGCCACGATAACGTTGCCCTGCTCGATGGTGGCTTCGGCAAATGGACCAAGGAGAAGC
AGCCGGCGGAGATGACGCCGGTGCGCCCGTCGCCGGCCAGCTTCACGGCCAGCTTCCGGCCCGAGCTGGTCGCGACCTCT
GCGCAGGTGCAGGGCGCGCTGCAAAGTGGCGGGCAGCTGGTCGACGCCCGCGGTCCGGGCAAGTTCGACGGCACGCAGCC
GGACGTTTTTTCCTTCAAGCGCCAGGGCCACATTCCCGGCGCCATCAACCTGCCCTGGGCCGATCTGGTCGATCCCGACA
CCGGCGTGCTGCTGCCGCCCGACGGACTTGCCGCGCGCTTCGCGGCCGCCGGTGTCGATCTGGCGAAGCCGATCGTCACC
ACCTGCGCCTCGGGCATCATCTCCTGCATGCTGGCGCTGGCGCTGTACCGGCTCGGCGTTCCCACCGTCGCGGTCCACGA
CGGCGCCTGGGCGGAATGGAGCCAGCTCGACGACACGCCGGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TATTACGACAGCCATCCGCGCCCGGGCTACGCGATGCCGAAAACGCTCTGACGCGCTTTTCGCGTCAGCTGTCCCGTTGG
CCCTCATCGAGAGCGCATGC

Downstream 100 bases:

>100_bases
CCTCTGGACCTCGATGACGGGAGCCCACGCATGACAAAGACTCTGGTCAAGACCACGCAGGACGGCCGCAAGGTGGAAGT
GATCGGGCTGGCCATCTGCC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSRDPSDALVSSEWLAQHLSDPDIKILDCTWHHPSTNLDGRTQYRGRHLPGSVHFDIDQVADKTNPLPHMLPTAEDFAHK
VGLLGISNADRVIVYDRHYGGSAAGRVWWMFRVFGHDNVALLDGGFGKWTKEKQPAEMTPVRPSPASFTASFRPELVATS
AQVQGALQSGGQLVDARGPGKFDGTQPDVFSFKRQGHIPGAINLPWADLVDPDTGVLLPPDGLAARFAAAGVDLAKPIVT
TCASGIISCMLALALYRLGVPTVAVHDGAWAEWSQLDDTPAAA

Sequences:

>Translated_283_residues
MSRDPSDALVSSEWLAQHLSDPDIKILDCTWHHPSTNLDGRTQYRGRHLPGSVHFDIDQVADKTNPLPHMLPTAEDFAHK
VGLLGISNADRVIVYDRHYGGSAAGRVWWMFRVFGHDNVALLDGGFGKWTKEKQPAEMTPVRPSPASFTASFRPELVATS
AQVQGALQSGGQLVDARGPGKFDGTQPDVFSFKRQGHIPGAINLPWADLVDPDTGVLLPPDGLAARFAAAGVDLAKPIVT
TCASGIISCMLALALYRLGVPTVAVHDGAWAEWSQLDDTPAAA
>Mature_282_residues
SRDPSDALVSSEWLAQHLSDPDIKILDCTWHHPSTNLDGRTQYRGRHLPGSVHFDIDQVADKTNPLPHMLPTAEDFAHKV
GLLGISNADRVIVYDRHYGGSAAGRVWWMFRVFGHDNVALLDGGFGKWTKEKQPAEMTPVRPSPASFTASFRPELVATSA
QVQGALQSGGQLVDARGPGKFDGTQPDVFSFKRQGHIPGAINLPWADLVDPDTGVLLPPDGLAARFAAAGVDLAKPIVTT
CASGIISCMLALALYRLGVPTVAVHDGAWAEWSQLDDTPAAA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=44.6886446886447, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=41.1764705882353, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=41.1764705882353, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=41.1764705882353, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI87082121, Length=274, Percent_Identity=38.6861313868613, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87081967, Length=273, Percent_Identity=27.1062271062271, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=294, Percent_Identity=25.5102040816327, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71997283, Length=304, Percent_Identity=26.6447368421053, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17559150, Length=240, Percent_Identity=27.0833333333333, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=197, Percent_Identity=30.9644670050761, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543836, Length=277, Percent_Identity=25.2707581227437, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17543838, Length=277, Percent_Identity=25.2707581227437, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=268, Percent_Identity=33.955223880597, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30431; Mature: 30299

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDPSDALVSSEWLAQHLSDPDIKILDCTWHHPSTNLDGRTQYRGRHLPGSVHFDIDQV
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHH
ADKTNPLPHMLPTAEDFAHKVGLLGISNADRVIVYDRHYGGSAAGRVWWMFRVFGHDNVA
HHCCCCCCCCCCCHHHHHHHHCEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEE
LLDGGFGKWTKEKQPAEMTPVRPSPASFTASFRPELVATSAQVQGALQSGGQLVDARGPG
EEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCC
KFDGTQPDVFSFKRQGHIPGAINLPWADLVDPDTGVLLPPDGLAARFAAAGVDLAKPIVT
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEECCCCHHHHHHHCCCHHHHHHHH
TCASGIISCMLALALYRLGVPTVAVHDGAWAEWSQLDDTPAAA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SRDPSDALVSSEWLAQHLSDPDIKILDCTWHHPSTNLDGRTQYRGRHLPGSVHFDIDQV
CCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHH
ADKTNPLPHMLPTAEDFAHKVGLLGISNADRVIVYDRHYGGSAAGRVWWMFRVFGHDNVA
HHCCCCCCCCCCCHHHHHHHHCEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEE
LLDGGFGKWTKEKQPAEMTPVRPSPASFTASFRPELVATSAQVQGALQSGGQLVDARGPG
EEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCC
KFDGTQPDVFSFKRQGHIPGAINLPWADLVDPDTGVLLPPDGLAARFAAAGVDLAKPIVT
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEECCCCHHHHHHHCCCHHHHHHHH
TCASGIISCMLALALYRLGVPTVAVHDGAWAEWSQLDDTPAAA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]