Click here to switch to the map view.

The map label for this gene is nifB [H]

Identifier: 146342266

GI number: 146342266

Start: 5610752

End: 5612314

Strand: Reverse

Name: nifB [H]

Synonym: BRADO5420

Alternate gene names: 146342266

Gene position: 5612314-5610752 (Counterclockwise)

Preceding gene: 146342267

Following gene: 146342265

Centisome position: 75.27

GC content: 65.64

Gene sequence:

>1563_bases
ATGGACGCGTCAGTGCAGCACGAACAGGCGACGATACCGGACGGCGACAAGCTGAGCTCGGTGATGCAGACCATCGCAGA
ACATAAGGGCTGCGGCACCTCGGGCGGCAGCGGCAAAGCGAGCTGCGGCTCGGGCGCCGGCGAGAACGACCTCCCGCCGG
AGATCTGGGAGAAGGTGAAGAACCATCCCTGCTACAGCGAGGAGGCGCATCACCACTACGCGCGCATGCATGTCGCCGTG
GCGCCCGCCTGCAACATCCAGTGCAACTATTGCAATCGCAAATATGACTGCGCCAACGAGTCGCGTCCGGGCGTCGTCAG
CGAGAAGCTCTCGCCGGAGCAGGCTGCCAAGAAGGTGCTCGCGGTCGCCTCGACCATTCCGCAGATGACCGTGCTCGGCA
TCGCCGGCCCCGGCGATCCGCTCGCCAACCCCGAGAAGACCTTCAAGACGTTCGAGCTGATCGCGCGCACCGCGCCCGAC
ATCAAGCTCTGCCTGTCGACCAACGGCCTCGCGCTGCCCGACCATGTCGACACCATCGCCGGCTTCAACGTCGACCACGT
CACCATCACCATCAACATGGTCGATCCCGAGATCGGCGCCAAGATCTATCCCTGGGTGTTCTGGAAGCACAAGCGCTACA
CCGGCGTCGAGGCGGCCAAGCTGCTGACCGACCGCCAGCTGCAGGGCCTGGAGATGCTGACCGAGCGCGGCATCCTCTGC
AAGGTCAACTCGGTGATGATCCCCGGCGTCAACGACAAGCACCTCGTCGAGGTCAACAAGGCGGTGAAGTCGCGCGGCGC
CTTCCTGCACAACATCATGCCGCTGATCTCCTCGCCGGAGCATGGCACCGTGTTCGGCCTGACCGGCCAGCGCGGCCCGA
CCGCGCAGGAGCTGAAGGCGCTGCAGGACGAGTGCGAAGGCGAGATGAACATGATGCGGCACTGCCGCCAGTGCCGCGCC
GACGCCGTCGGCCTGCTCGGCGAGGACCGCTCGGCGGAGTTCACCACCGACAAGATCATGGCGATGGAAGTGAACTACGA
CATCGACAGCCGCAAGGCCTATCAGGAGGCGGTCGAGAGCGAGCGTGTCGCCAAGGTCGTTGCCCGCCAGGAGGAGCTTG
CCTCGCTCGCCGGCCAGTCCAGCGACGTCAAGCTGCTGGTCGCGGTCGCGACCAAGGGCTCGGGCCTGATCAACGAGCAT
TTCGGCCACGCCAAGGAGTTCCAGGTCTACGAACTCTCCACGGCCGGTGCGAAGTTCGTCGGTCACCGCCGTGTCGACCT
GTACTGCCAGGGCGGTTATGGCGACGAGGACAGCCTGGAGACCGTGATCCGCGCCATCAACGACTGCCATGCGGTGTTCG
TGGCCAAGATCGGCGGCTGCCCGAAGAGCGACCTGCAGGCCGCCGGCATCGAGCCGGTCGACCAGTTCGCCCACGAGTTC
ATCGAGAAGTCGGCGATCGCCTGGTTCAAGAGCTACCTGGACAAGGTCAACAGCGGCGAGATCGAGCACGTCGTGCGCGG
GGACGCCGAGATCCGCCAGGGCGCCCTGACCTCGGCGGCGTGA

Upstream 100 bases:

>100_bases
GATAGTCCGAAGAAATGATCTTGTCCGCGGCGTGGCACGCGAGTTGCTGAACTCCTTACCAAGGCGGTTTGCGGCTCGCC
AGCAACGTTAAGGAGCGGAC

Downstream 100 bases:

>100_bases
GATCCGATCCCGGCGGCGTCGCGGCGCCGCCGGCCCCTTTCGACAGGAGCGTTTCATGACTTTGAAGATCATTGCCTCGC
AATGCACGAGCTGTTCGGCC

Product: nitrogenase FeMo cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MDASVQHEQATIPDGDKLSSVMQTIAEHKGCGTSGGSGKASCGSGAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIARTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEIGAKIYPWVFWKHKRYTGVEAAKLLTDRQLQGLEMLTERGILC
KVNSVMIPGVNDKHLVEVNKAVKSRGAFLHNIMPLISSPEHGTVFGLTGQRGPTAQELKALQDECEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDIDSRKAYQEAVESERVAKVVARQEELASLAGQSSDVKLLVAVATKGSGLINEH
FGHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGDEDSLETVIRAINDCHAVFVAKIGGCPKSDLQAAGIEPVDQFAHEF
IEKSAIAWFKSYLDKVNSGEIEHVVRGDAEIRQGALTSAA

Sequences:

>Translated_520_residues
MDASVQHEQATIPDGDKLSSVMQTIAEHKGCGTSGGSGKASCGSGAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIARTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEIGAKIYPWVFWKHKRYTGVEAAKLLTDRQLQGLEMLTERGILC
KVNSVMIPGVNDKHLVEVNKAVKSRGAFLHNIMPLISSPEHGTVFGLTGQRGPTAQELKALQDECEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDIDSRKAYQEAVESERVAKVVARQEELASLAGQSSDVKLLVAVATKGSGLINEH
FGHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGDEDSLETVIRAINDCHAVFVAKIGGCPKSDLQAAGIEPVDQFAHEF
IEKSAIAWFKSYLDKVNSGEIEHVVRGDAEIRQGALTSAA
>Mature_520_residues
MDASVQHEQATIPDGDKLSSVMQTIAEHKGCGTSGGSGKASCGSGAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIARTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEIGAKIYPWVFWKHKRYTGVEAAKLLTDRQLQGLEMLTERGILC
KVNSVMIPGVNDKHLVEVNKAVKSRGAFLHNIMPLISSPEHGTVFGLTGQRGPTAQELKALQDECEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDIDSRKAYQEAVESERVAKVVARQEELASLAGQSSDVKLLVAVATKGSGLINEH
FGHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGDEDSLETVIRAINDCHAVFVAKIGGCPKSDLQAAGIEPVDQFAHEF
IEKSAIAWFKSYLDKVNSGEIEHVVRGDAEIRQGALTSAA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56468; Mature: 56468

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASVQHEQATIPDGDKLSSVMQTIAEHKGCGTSGGSGKASCGSGAGENDLPPEIWEKVK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVL
CCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
AVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIARTAPDIKLCLSTNGLALPDHVDTIA
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
GFNVDHVTITINMVDPEIGAKIYPWVFWKHKRYTGVEAAKLLTDRQLQGLEMLTERGILC
CCCEEEEEEEEEEECCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
KVNSVMIPGVNDKHLVEVNKAVKSRGAFLHNIMPLISSPEHGTVFGLTGQRGPTAQELKA
EECCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
LQDECEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDIDSRKAYQEAVES
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ERVAKVVARQEELASLAGQSSDVKLLVAVATKGSGLINEHFGHAKEFQVYELSTAGAKFV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHH
GHRRVDLYCQGGYGDEDSLETVIRAINDCHAVFVAKIGGCPKSDLQAAGIEPVDQFAHEF
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHH
IEKSAIAWFKSYLDKVNSGEIEHVVRGDAEIRQGALTSAA
HHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCC
>Mature Secondary Structure
MDASVQHEQATIPDGDKLSSVMQTIAEHKGCGTSGGSGKASCGSGAGENDLPPEIWEKVK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVL
CCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
AVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIARTAPDIKLCLSTNGLALPDHVDTIA
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
GFNVDHVTITINMVDPEIGAKIYPWVFWKHKRYTGVEAAKLLTDRQLQGLEMLTERGILC
CCCEEEEEEEEEEECCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
KVNSVMIPGVNDKHLVEVNKAVKSRGAFLHNIMPLISSPEHGTVFGLTGQRGPTAQELKA
EECCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
LQDECEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDIDSRKAYQEAVES
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ERVAKVVARQEELASLAGQSSDVKLLVAVATKGSGLINEHFGHAKEFQVYELSTAGAKFV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHH
GHRRVDLYCQGGYGDEDSLETVIRAINDCHAVFVAKIGGCPKSDLQAAGIEPVDQFAHEF
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHH
IEKSAIAWFKSYLDKVNSGEIEHVVRGDAEIRQGALTSAA
HHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]