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The map label for this gene is nifV [H]

Identifier: 146342237

GI number: 146342237

Start: 5594597

End: 5595736

Strand: Reverse

Name: nifV [H]

Synonym: BRADO5390

Alternate gene names: 146342237

Gene position: 5595736-5594597 (Counterclockwise)

Preceding gene: 146342238

Following gene: 146342236

Centisome position: 75.04

GC content: 67.72

Gene sequence:

>1140_bases
ATGCGCCGGGTTATCCTGAACGACACCACGCTTCGCGACGGCGAGCAGGCCCCGGGCGTCGCTTTCGCCCTTACCGAGAA
GGTCGCGATCGCCCGCGCGCTGGCGCGGGCCGGCGTTCCCGAGATCGAAGCCGGCACGCCCGCGATGGGGGCCGACGAGA
TCGCCGCGATCAGGGCAATCGTCGAGGCCGGTCTGCCGGCGACCATCATCGGCTGGTGCCGGATGCGCGAGGCGGATGTC
GATGCCGCCGTCGCAGCCGGTGTGTCGATGGTCAATGTCTCCATTCCGACGTCGGATGTTCAGATCGCAGCCAAGCTCGG
CGGTGGCCGCAGCGTCGCGCTGGAATTGCTCAAGCGCGTTGTCAGCTATGCAAAGAGCCGGGGATTGGACGTTGCCGTCG
GCGGCGAGGATTCCTCGCGCGCGGACGTCGGCTTCCTGGCGGAAGTGATTGCGACCGCAAAGGCGCTCGGCGCGCGGCGC
TTCCGTGTCGCCGATACCCTGAGCGTGCTCGATCCCGACGCGACATCGGCGCTGGTCGGAGCCTTGCGCGCGACGACCGA
CCTGGAGATCGAATTTCACGGCCACGATGATCTCGGCCTTGCCACCGCCAACACGCTCGCCGCCGTGAAGGCCGGCGCGA
CGCATGCGTCGGTCACGGTGATCGGCCTCGGCGAGCGCGCCGGCAATGCGCCGCTGGAGGAGGTGGCGGTCGCGTTGCGG
CAGCTCTACCGTCGCGAGACCGGCATCGTGCTCGCCGAGCTCGATCATGTCGCCAATGTCGTCGCAGCCGCTGCGGCGCG
CGCGATCCCGCTCAACAAGGCGATCGTTGGCGAGCATGTCTTCACCCACGAGTCCGGTATTCACGTCGATGGCCTGCTCA
AGGATCAGCGCACCTACCAGGCGCTCGATCCCATGCTGTTCGGCCGTTCCAACCGGATCGTGATCGGCAAGCATTCCGGT
CTCTCGGCGATCACCTCGCTGCTGTCCGACCTGCGTCTGCCGGCGAGCGCCGACCAGGCGCAACGCATCCTCGCGCGCGT
GCGCAAGCATGCGGTCGCGCACAAGGGTCCTGTGCCGAAGGAAGCCGTTGCCGCCATCTGGCGCGAAGTTTGTGATCACC
CGCATGCGAGCTTTGCCTGA

Upstream 100 bases:

>100_bases
CTTCGGACCACGCGAGCCCAAGAATGTCAGCAGGAATGGCCGGGAGGCCGGCTCTGCGCTCGGGAGCAAGGGTCTTGTGC
GTTCCACGTCCGGGAGCCCG

Downstream 100 bases:

>100_bases
TCATGACCGACATCATGGATAACCCGATTGCCAAGAGCGCTGCCGCCGACGCGAGCCCGCCGTCGCTGTTCCGCCTCATC
CGCGAGGACATCGGCTGCGT

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRRVILNDTTLRDGEQAPGVAFALTEKVAIARALARAGVPEIEAGTPAMGADEIAAIRAIVEAGLPATIIGWCRMREADV
DAAVAAGVSMVNVSIPTSDVQIAAKLGGGRSVALELLKRVVSYAKSRGLDVAVGGEDSSRADVGFLAEVIATAKALGARR
FRVADTLSVLDPDATSALVGALRATTDLEIEFHGHDDLGLATANTLAAVKAGATHASVTVIGLGERAGNAPLEEVAVALR
QLYRRETGIVLAELDHVANVVAAAAARAIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQALDPMLFGRSNRIVIGKHSG
LSAITSLLSDLRLPASADQAQRILARVRKHAVAHKGPVPKEAVAAIWREVCDHPHASFA

Sequences:

>Translated_379_residues
MRRVILNDTTLRDGEQAPGVAFALTEKVAIARALARAGVPEIEAGTPAMGADEIAAIRAIVEAGLPATIIGWCRMREADV
DAAVAAGVSMVNVSIPTSDVQIAAKLGGGRSVALELLKRVVSYAKSRGLDVAVGGEDSSRADVGFLAEVIATAKALGARR
FRVADTLSVLDPDATSALVGALRATTDLEIEFHGHDDLGLATANTLAAVKAGATHASVTVIGLGERAGNAPLEEVAVALR
QLYRRETGIVLAELDHVANVVAAAAARAIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQALDPMLFGRSNRIVIGKHSG
LSAITSLLSDLRLPASADQAQRILARVRKHAVAHKGPVPKEAVAAIWREVCDHPHASFA
>Mature_379_residues
MRRVILNDTTLRDGEQAPGVAFALTEKVAIARALARAGVPEIEAGTPAMGADEIAAIRAIVEAGLPATIIGWCRMREADV
DAAVAAGVSMVNVSIPTSDVQIAAKLGGGRSVALELLKRVVSYAKSRGLDVAVGGEDSSRADVGFLAEVIATAKALGARR
FRVADTLSVLDPDATSALVGALRATTDLEIEFHGHDDLGLATANTLAAVKAGATHASVTVIGLGERAGNAPLEEVAVALR
QLYRRETGIVLAELDHVANVVAAAAARAIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQALDPMLFGRSNRIVIGKHSG
LSAITSLLSDLRLPASADQAQRILARVRKHAVAHKGPVPKEAVAAIWREVCDHPHASFA

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=350, Percent_Identity=33.4285714285714, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320071, Length=354, Percent_Identity=32.7683615819209, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320019, Length=349, Percent_Identity=32.6647564469914, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 39550; Mature: 39550

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVILNDTTLRDGEQAPGVAFALTEKVAIARALARAGVPEIEAGTPAMGADEIAAIRAI
CCEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
VEAGLPATIIGWCRMREADVDAAVAAGVSMVNVSIPTSDVQIAAKLGGGRSVALELLKRV
HHCCCCHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
VSYAKSRGLDVAVGGEDSSRADVGFLAEVIATAKALGARRFRVADTLSVLDPDATSALVG
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
ALRATTDLEIEFHGHDDLGLATANTLAAVKAGATHASVTVIGLGERAGNAPLEEVAVALR
HHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
QLYRRETGIVLAELDHVANVVAAAAARAIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQ
HHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCHHHHCCHHEECCCCCEEHHHHHHHHHHH
ALDPMLFGRSNRIVIGKHSGLSAITSLLSDLRLPASADQAQRILARVRKHAVAHKGPVPK
HHCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
EAVAAIWREVCDHPHASFA
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRVILNDTTLRDGEQAPGVAFALTEKVAIARALARAGVPEIEAGTPAMGADEIAAIRAI
CCEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
VEAGLPATIIGWCRMREADVDAAVAAGVSMVNVSIPTSDVQIAAKLGGGRSVALELLKRV
HHCCCCHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
VSYAKSRGLDVAVGGEDSSRADVGFLAEVIATAKALGARRFRVADTLSVLDPDATSALVG
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
ALRATTDLEIEFHGHDDLGLATANTLAAVKAGATHASVTVIGLGERAGNAPLEEVAVALR
HHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
QLYRRETGIVLAELDHVANVVAAAAARAIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQ
HHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCHHHHCCHHEECCCCCEEHHHHHHHHHHH
ALDPMLFGRSNRIVIGKHSGLSAITSLLSDLRLPASADQAQRILARVRKHAVAHKGPVPK
HHCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
EAVAAIWREVCDHPHASFA
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9139910; 11759840 [H]