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The map label for this gene is siaT [H]

Identifier: 146341657

GI number: 146341657

Start: 4954203

End: 4956062

Strand: Reverse

Name: siaT [H]

Synonym: BRADO4761

Alternate gene names: 146341657

Gene position: 4956062-4954203 (Counterclockwise)

Preceding gene: 146341658

Following gene: 146341656

Centisome position: 66.47

GC content: 68.44

Gene sequence:

>1860_bases
ATGAACACGGTCGACGCGTCTCTGGTTGCCCCCACGAAGCACGGCATCGTCGAAACCCTCGAACGGGTGCTGACGAAGCT
GATCGAGATTCCCGCGATCGTCGCGCTGGTCGCGGAAGTCGGAATCCTGTTCACCGGCGTGACGGCGCGTTTCGCCTTCA
ACCGGCCGCTGACCTGGGCCGATGAGCTCGCGTCGATCACCTTTCTCTGGCTCGCGATGCTGGGCGCGGTGCTCGCGCTC
AGCCGCGGCGAGCACATGCGGATGACCGCATTGATCGACCGCGTCGGACCGCGCACCCGCGCGGTGCTGGAGAGCGCGGC
GCTGATGGCGCCGGCGCTGTTCCTGGCGATCCTGCTGCACCCCGCCATCGACTATGCGAGCGGGCAGGCGTTCGCGGAAA
CGCCGGCGCTCGGCCTGCCCGACAGCGTGCGGGCCGGCGCGGTGCCGGTCGGCATCGGTCTCATGCTCGCCACCGCCTTG
CTGCGCATGGCCCGCCATGGCTCGCGCGACATCGCGATCACGCTCGTGATCCTGGCGGCCACCGCGCTCGTGTTGTGGGG
TGCGGCGCCGGCGCTCAAGGCGCTCGGCAATTGGAATCTGATCGTCTTCTTCGCGATCCTGCTCGGCGCGGCCGTGCTCG
CCGGCGTGCCGATCGCGTTCTGCTTCGGCCTCGCCACGGTCGCCTATCTGCTCACGGTGACGTCGGCGCCGCTCGAGGTC
GTGACCAGCCGCATCGACGAGGGCGTCTCATCGCTGGTGCTGCTCGCAATCCCCTTGTTCATCCTGCTCGGCCACCTCAT
CGTGATGACCGGCATGGCCGCGGCCATGGTGGGCTTCCTGGTCTCGCTGGTCGGCCATGTCCGGGGCGGCCTGTCCTACG
TGCTGCTCGGTGCGATCCTGCTGGTGTCCGGCATCTCCGGCGCCAAGACGGCCGACATGGCCGCGGTCGCGCCGGTGCTG
TTTCCCGACATGAAGCGCCGCGGCATTCATGACGGCGAGCTGGTGTCGCTGCTCGCGGCCTCCGGCGCCATGGCGGAGAC
CATTCCGCCATCGATCGTGCTGATCATCATCGGCTCGGTGGCCGGGGTGTCGATCTCCGCGCTGTTCACCGGCGGCATCC
TGCCGGGCCTGGTGCTGGCCGCGGCGCTCGCGATCGTCGCCAAGCGCCGCATGGTCGAGCCCGAAGGCATCGTCCGGCAG
CGTCCCTCCTGGGCCACGATCCGCGCCGCGCTGATCACCGCACTGCCGGCGCTGCTGCTACCGATCCTGATCCGCACCGC
GGTCACCGAGGGCGTCGCCACCGCGACCGAAGTCTCGACCATCGGCATCGCCTACACCGTGCTGGTCGGTCTGCTCGTCT
ATCGCCGCTTCGACTGGCGCAAGCTGTATCCTGCGCTGGTGCATACCGCGGCGTTGTCGGGATCGATCCTGTTCATCATC
GGCGCGGCCACCGCGATGGCCTGGGCGCTGACGCAGTCCAACTTCTCGCACGCGCTCGCGGCGATGATGGTGTCGGCCCC
GGGCGGCAAATACGGATTCCTGCTGATCTCGATCGCCGTGTTCATCCTGCTCGGCAGCATCCTCGAAGGCCTGCCGGCGA
TGGTGCTGTTTGGCCCGTTGCTATTCCCGGCGGCCAAGCTGCTCGGCGTGCATGAGGTGCACTACGCGATGGTGATCATC
CTCGCCATGGGCATCGGCCTGTTCGCGCCGCCCTTCGGCCTCGGCTACTACGCCGCGTGCTCGATCGGGCAGATCGATCC
CAACGCCGGACTGACGCGGATCTGGCCCTACCTCGGCGCGCTCGTGCTCGGCCTGCTGCTCGTCGCGTTCGTGCCGTGGC
TGTCGATTGGATTTCTCTGA

Upstream 100 bases:

>100_bases
TCCGGACCGCACTCGGCAAGACCAGCTTCTACAAGGACTGGCGCGGCAAGTTCGGCGAGGATGCCTGGAAGATCCTGCAG
TCGAACGTGGGAGAGCTCGG

Downstream 100 bases:

>100_bases
TCCGCGCGGCGGAGGCACAGCCGCATGCAACACACAGCCTTGCGCCGCGCCCGCGGCGCGGGGCATCCTGGCGCAAAGTG
ACACCGTCTGAAGGACCCCT

Product: TRAP-type C4-dicarboxylate transporter small and large permease

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNTVDASLVAPTKHGIVETLERVLTKLIEIPAIVALVAEVGILFTGVTARFAFNRPLTWADELASITFLWLAMLGAVLAL
SRGEHMRMTALIDRVGPRTRAVLESAALMAPALFLAILLHPAIDYASGQAFAETPALGLPDSVRAGAVPVGIGLMLATAL
LRMARHGSRDIAITLVILAATALVLWGAAPALKALGNWNLIVFFAILLGAAVLAGVPIAFCFGLATVAYLLTVTSAPLEV
VTSRIDEGVSSLVLLAIPLFILLGHLIVMTGMAAAMVGFLVSLVGHVRGGLSYVLLGAILLVSGISGAKTADMAAVAPVL
FPDMKRRGIHDGELVSLLAASGAMAETIPPSIVLIIIGSVAGVSISALFTGGILPGLVLAAALAIVAKRRMVEPEGIVRQ
RPSWATIRAALITALPALLLPILIRTAVTEGVATATEVSTIGIAYTVLVGLLVYRRFDWRKLYPALVHTAALSGSILFII
GAATAMAWALTQSNFSHALAAMMVSAPGGKYGFLLISIAVFILLGSILEGLPAMVLFGPLLFPAAKLLGVHEVHYAMVII
LAMGIGLFAPPFGLGYYAACSIGQIDPNAGLTRIWPYLGALVLGLLLVAFVPWLSIGFL

Sequences:

>Translated_619_residues
MNTVDASLVAPTKHGIVETLERVLTKLIEIPAIVALVAEVGILFTGVTARFAFNRPLTWADELASITFLWLAMLGAVLAL
SRGEHMRMTALIDRVGPRTRAVLESAALMAPALFLAILLHPAIDYASGQAFAETPALGLPDSVRAGAVPVGIGLMLATAL
LRMARHGSRDIAITLVILAATALVLWGAAPALKALGNWNLIVFFAILLGAAVLAGVPIAFCFGLATVAYLLTVTSAPLEV
VTSRIDEGVSSLVLLAIPLFILLGHLIVMTGMAAAMVGFLVSLVGHVRGGLSYVLLGAILLVSGISGAKTADMAAVAPVL
FPDMKRRGIHDGELVSLLAASGAMAETIPPSIVLIIIGSVAGVSISALFTGGILPGLVLAAALAIVAKRRMVEPEGIVRQ
RPSWATIRAALITALPALLLPILIRTAVTEGVATATEVSTIGIAYTVLVGLLVYRRFDWRKLYPALVHTAALSGSILFII
GAATAMAWALTQSNFSHALAAMMVSAPGGKYGFLLISIAVFILLGSILEGLPAMVLFGPLLFPAAKLLGVHEVHYAMVII
LAMGIGLFAPPFGLGYYAACSIGQIDPNAGLTRIWPYLGALVLGLLLVAFVPWLSIGFL
>Mature_619_residues
MNTVDASLVAPTKHGIVETLERVLTKLIEIPAIVALVAEVGILFTGVTARFAFNRPLTWADELASITFLWLAMLGAVLAL
SRGEHMRMTALIDRVGPRTRAVLESAALMAPALFLAILLHPAIDYASGQAFAETPALGLPDSVRAGAVPVGIGLMLATAL
LRMARHGSRDIAITLVILAATALVLWGAAPALKALGNWNLIVFFAILLGAAVLAGVPIAFCFGLATVAYLLTVTSAPLEV
VTSRIDEGVSSLVLLAIPLFILLGHLIVMTGMAAAMVGFLVSLVGHVRGGLSYVLLGAILLVSGISGAKTADMAAVAPVL
FPDMKRRGIHDGELVSLLAASGAMAETIPPSIVLIIIGSVAGVSISALFTGGILPGLVLAAALAIVAKRRMVEPEGIVRQ
RPSWATIRAALITALPALLLPILIRTAVTEGVATATEVSTIGIAYTVLVGLLVYRRFDWRKLYPALVHTAALSGSILFII
GAATAMAWALTQSNFSHALAAMMVSAPGGKYGFLLISIAVFILLGSILEGLPAMVLFGPLLFPAAKLLGVHEVHYAMVII
LAMGIGLFAPPFGLGYYAACSIGQIDPNAGLTRIWPYLGALVLGLLLVAFVPWLSIGFL

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=380, Percent_Identity=29.4736842105263, Blast_Score=132, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 64421; Mature: 64421

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVDASLVAPTKHGIVETLERVLTKLIEIPAIVALVAEVGILFTGVTARFAFNRPLTWA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DELASITFLWLAMLGAVLALSRGEHMRMTALIDRVGPRTRAVLESAALMAPALFLAILLH
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
PAIDYASGQAFAETPALGLPDSVRAGAVPVGIGLMLATALLRMARHGSRDIAITLVILAA
HHHHHCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TALVLWGAAPALKALGNWNLIVFFAILLGAAVLAGVPIAFCFGLATVAYLLTVTSAPLEV
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VTSRIDEGVSSLVLLAIPLFILLGHLIVMTGMAAAMVGFLVSLVGHVRGGLSYVLLGAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVSGISGAKTADMAAVAPVLFPDMKRRGIHDGELVSLLAASGAMAETIPPSIVLIIIGSV
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHH
AGVSISALFTGGILPGLVLAAALAIVAKRRMVEPEGIVRQRPSWATIRAALITALPALLL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
PILIRTAVTEGVATATEVSTIGIAYTVLVGLLVYRRFDWRKLYPALVHTAALSGSILFII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEH
GAATAMAWALTQSNFSHALAAMMVSAPGGKYGFLLISIAVFILLGSILEGLPAMVLFGPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPAAKLLGVHEVHYAMVIILAMGIGLFAPPFGLGYYAACSIGQIDPNAGLTRIWPYLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
LVLGLLLVAFVPWLSIGFL
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTVDASLVAPTKHGIVETLERVLTKLIEIPAIVALVAEVGILFTGVTARFAFNRPLTWA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DELASITFLWLAMLGAVLALSRGEHMRMTALIDRVGPRTRAVLESAALMAPALFLAILLH
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
PAIDYASGQAFAETPALGLPDSVRAGAVPVGIGLMLATALLRMARHGSRDIAITLVILAA
HHHHHCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TALVLWGAAPALKALGNWNLIVFFAILLGAAVLAGVPIAFCFGLATVAYLLTVTSAPLEV
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VTSRIDEGVSSLVLLAIPLFILLGHLIVMTGMAAAMVGFLVSLVGHVRGGLSYVLLGAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVSGISGAKTADMAAVAPVLFPDMKRRGIHDGELVSLLAASGAMAETIPPSIVLIIIGSV
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHH
AGVSISALFTGGILPGLVLAAALAIVAKRRMVEPEGIVRQRPSWATIRAALITALPALLL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
PILIRTAVTEGVATATEVSTIGIAYTVLVGLLVYRRFDWRKLYPALVHTAALSGSILFII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEH
GAATAMAWALTQSNFSHALAAMMVSAPGGKYGFLLISIAVFILLGSILEGLPAMVLFGPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPAAKLLGVHEVHYAMVIILAMGIGLFAPPFGLGYYAACSIGQIDPNAGLTRIWPYLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
LVLGLLLVAFVPWLSIGFL
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]