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The map label for this gene is pbuE [H]

Identifier: 146341543

GI number: 146341543

Start: 4831779

End: 4832963

Strand: Reverse

Name: pbuE [H]

Synonym: BRADO4641

Alternate gene names: 146341543

Gene position: 4832963-4831779 (Counterclockwise)

Preceding gene: 146341544

Following gene: 146341542

Centisome position: 64.81

GC content: 71.9

Gene sequence:

>1185_bases
ATGCCCTTCGCACTGTTCGCCCTCGCCATGATCAACTTCGCCGTGGGCACGCAAGGCTTCGCCTTCGCGGGCGTGCTCTC
CGAGCTCTCCCGTGATCTCGGGGTCACCATCGGCCAGGCCGGCCTGATCGTCGGCGCGTCGTCGATCACCTTCGCGCTCG
GCGCTCCCTTTGCCGCCGCCGCCGTCGCGCAGATCGAACGCCGCCGCGTCATCGTCACCGGCCTTGCCGCACTGGCACTC
GTCAATCTGCTGTGCGCGGTCGCGCCGTCCTTTGCCGCGCTCACGGCGCTGCGCATCGCGGCCGGGCTGGCCACCGCATT
CGTCGGCGCGCTGGCGACCGTCGCCGCGGCCTCGCTCGTTGCGCCGGAGAAGCGGGGCCGGGCATTCGCGATCGTGATGG
GCGGGCTGACGGTCGCCTTCGTGCTCGGCGTCCCGCTCGGCTCGGTGATCGGCGGTCTCTATGGCTGGCGCGCGACCTTT
GCGATGTCGGCCATGGTCTGCCTGGGCTCGGTCGTGCTGGTTGCGCTCACGGTGCCCGCGATCGCACCGGCTGCCGGCGC
ACGTCCGCGCCTCGGCGACGTCCTGGGCAACGGCCGGGTCGTGCGGGTGCTGATGCTCACCCTGATCGGATTTGCCGCGA
CCTTCACCGTGGTGTCCTTTGTCGGCCCGGTGATCACCGCGACCACCGGCTTGACGGGCGCCAGGATCGGCGCGTTGCAG
GCCTTCATCGGTTTCGGCTCGCTCGTTGGGCTGGCGCTCGGCGGCATCGCCAGCGACCGCGGCCGCGGTCCGGTCGCGGC
GGCCGTCGCCTGTGCCGCGATGTCCTTGAGCCTGTTGCTGTACTGGCTCGCGCTGTCGGCGCCGCACGCCACCGTGCCGG
CGCTGGCAATGGGTCTCCTGATGTTCGCCGGTGCGACGGCACTGTTCTCGCTCATCCCGCTCAATCTCGCCGCGATCACG
CTGCACGCCGGGACGTCGGCGCCAGTTGCGCTCGCGCTCAACGGCTCGCTGGTCTCGCTGGGACAGGGCCTCGGCGCGCT
GCTCGGCGGTGCGCTCACCGACGCGTTCGGGGCGGCGGCGATGGGGCCGGGTGGAGCGGTGCTGGCGGGAATGGGTTTCT
TGCTCGCGCTCACCATCGCGCGCCGCGACGCCGCCCCCGCCGTCAAGCTCGTGGCAGCCGAGTGA

Upstream 100 bases:

>100_bases
GATGCAGAATCCGAGCTGGCGCATCGCGCGCGGCGGCACTCACAACGTGTCCGTCATCGCTGCCTGACGCCGTCATTCAC
CCCAACCAGAGGCCCGTATC

Downstream 100 bases:

>100_bases
ATGGGAGATACCGATGACCGCGACCGTCTTTCTCGTCTTCCTGTTTGTGGTGGTTGCGGCCGTTGCGATCGCTGTGAGCC
GCGCGCTGCCGCGCGGCGAA

Product: major facilitator superfamily permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPFALFALAMINFAVGTQGFAFAGVLSELSRDLGVTIGQAGLIVGASSITFALGAPFAAAAVAQIERRRVIVTGLAALAL
VNLLCAVAPSFAALTALRIAAGLATAFVGALATVAAASLVAPEKRGRAFAIVMGGLTVAFVLGVPLGSVIGGLYGWRATF
AMSAMVCLGSVVLVALTVPAIAPAAGARPRLGDVLGNGRVVRVLMLTLIGFAATFTVVSFVGPVITATTGLTGARIGALQ
AFIGFGSLVGLALGGIASDRGRGPVAAAVACAAMSLSLLLYWLALSAPHATVPALAMGLLMFAGATALFSLIPLNLAAIT
LHAGTSAPVALALNGSLVSLGQGLGALLGGALTDAFGAAAMGPGGAVLAGMGFLLALTIARRDAAPAVKLVAAE

Sequences:

>Translated_394_residues
MPFALFALAMINFAVGTQGFAFAGVLSELSRDLGVTIGQAGLIVGASSITFALGAPFAAAAVAQIERRRVIVTGLAALAL
VNLLCAVAPSFAALTALRIAAGLATAFVGALATVAAASLVAPEKRGRAFAIVMGGLTVAFVLGVPLGSVIGGLYGWRATF
AMSAMVCLGSVVLVALTVPAIAPAAGARPRLGDVLGNGRVVRVLMLTLIGFAATFTVVSFVGPVITATTGLTGARIGALQ
AFIGFGSLVGLALGGIASDRGRGPVAAAVACAAMSLSLLLYWLALSAPHATVPALAMGLLMFAGATALFSLIPLNLAAIT
LHAGTSAPVALALNGSLVSLGQGLGALLGGALTDAFGAAAMGPGGAVLAGMGFLLALTIARRDAAPAVKLVAAE
>Mature_393_residues
PFALFALAMINFAVGTQGFAFAGVLSELSRDLGVTIGQAGLIVGASSITFALGAPFAAAAVAQIERRRVIVTGLAALALV
NLLCAVAPSFAALTALRIAAGLATAFVGALATVAAASLVAPEKRGRAFAIVMGGLTVAFVLGVPLGSVIGGLYGWRATFA
MSAMVCLGSVVLVALTVPAIAPAAGARPRLGDVLGNGRVVRVLMLTLIGFAATFTVVSFVGPVITATTGLTGARIGALQA
FIGFGSLVGLALGGIASDRGRGPVAAAVACAAMSLSLLLYWLALSAPHATVPALAMGLLMFAGATALFSLIPLNLAAITL
HAGTSAPVALALNGSLVSLGQGLGALLGGALTDAFGAAAMGPGGAVLAGMGFLLALTIARRDAAPAVKLVAAE

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=262, Percent_Identity=33.969465648855, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786595, Length=287, Percent_Identity=25.7839721254355, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787808, Length=229, Percent_Identity=24.4541484716157, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693198, Length=335, Percent_Identity=24.7761194029851, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 38554; Mature: 38422

Theoretical pI: Translated: 11.69; Mature: 11.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFALFALAMINFAVGTQGFAFAGVLSELSRDLGVTIGQAGLIVGASSITFALGAPFAAA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEECCCCEEEECCHHHHHHCCCHHHH
AVAQIERRRVIVTGLAALALVNLLCAVAPSFAALTALRIAAGLATAFVGALATVAAASLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APEKRGRAFAIVMGGLTVAFVLGVPLGSVIGGLYGWRATFAMSAMVCLGSVVLVALTVPA
CHHHCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAPAAGARPRLGDVLGNGRVVRVLMLTLIGFAATFTVVSFVGPVITATTGLTGARIGALQ
HCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AFIGFGSLVGLALGGIASDRGRGPVAAAVACAAMSLSLLLYWLALSAPHATVPALAMGLL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MFAGATALFSLIPLNLAAITLHAGTSAPVALALNGSLVSLGQGLGALLGGALTDAFGAAA
HHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
MGPGGAVLAGMGFLLALTIARRDAAPAVKLVAAE
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
PFALFALAMINFAVGTQGFAFAGVLSELSRDLGVTIGQAGLIVGASSITFALGAPFAAA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEECCCCEEEECCHHHHHHCCCHHHH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CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA