BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

Click here to switch to the map view.

The map label for this gene is dkgB [H]

Identifier: 146341469

GI number: 146341469

Start: 4758954

End: 4759772

Strand: Reverse

Name: dkgB [H]

Synonym: BRADO4561

Alternate gene names: 146341469

Gene position: 4759772-4758954 (Counterclockwise)

Preceding gene: 146341470

Following gene: 146341468

Centisome position: 63.83

GC content: 64.84

Gene sequence:

>819_bases
ATGCTGATGATCGAGGCCAATGGTGCCAGGATTCCCGCTCTCGGGCTCGGGACCTGGGAGCTGCGCGGACGCACCTGCAC
CCGCATCGTCGAGCAGGCGCTGACGCTCGGCTACCGGCACATCGACACCGCCCAGGTCTATGAGAACGAGCACGAGGTCG
GGGAGGGCCTGCGGGCGTCGCGGGTCCGCCGCAACGAGATCTTCCTCACGACCAAGGTGTGGACGACGCATTTCGCGCCG
AACGATCTGGAGCGCTCGACCAAGGAGAGCCTGGTCCGCCTGCGCGTCTCCGAGGTCGATCTCCTGCTGCTGCACTGGCC
GAACTCGCATGTGCCGCTGGCCGAGACGCTCGGTGCGCTGGCGCATGCCAAAAAGCTTGGTCTCGCGCGCCACATCGGCG
TGTCCAACTTCACCGTGGCGCTGCTCGACGAAGCGGTGGCAGTCTCGCCGGAGCCGCTGGCCTGCAATCAGGTCGAGTAT
CATCCCTATCTCGATCAGACCAAGGTCCGGGAAGCCTGCGCCCGGCATGGCATCGCGCTCGTCGCCTACAGTCCGATCGC
CAAGGGCAAGATAAAGAACGATCCGACGCTGACCCGCATCGGCCGCGCCCATGGCAAGACGACGGCGCAGATCTGTCTGC
GCTGGCTGGTGCAGCAGAACGTCGTGGCGATCCCGCGCACCTCGAAGATCGAGCGGCTGTCAGAGAATCTCGACGTGTTC
GATTTCGAGCTGTCCGGGCAGGACATGGCCGACATCTTCGCGATGGGCAGTCCCGAGGGACGAATGACCAACTTCGCCTT
CGCGCCCAATTGGGATTGA

Upstream 100 bases:

>100_bases
TGTTCGAGGCCTTAGCTAAGACTTAAGCTAAAAACCGGTCCCGCGTGGCACGGTGGGCGTGTGGCATGCATAATCATGCA
CGCGCTTGGAGTGCACTTCC

Downstream 100 bases:

>100_bases
GCGACAACCGCGCCGGCATGCTAGGGTGAGGCCAGCGGGCGGGTGGCCCGCGCTCAATCCAGGCGATGATCGCCGTGACC
TCGCGTGCCGGAGGCGCGCC

Product: putative aldo/keto reductase 2, 5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLMIEANGARIPALGLGTWELRGRTCTRIVEQALTLGYRHIDTAQVYENEHEVGEGLRASRVRRNEIFLTTKVWTTHFAP
NDLERSTKESLVRLRVSEVDLLLLHWPNSHVPLAETLGALAHAKKLGLARHIGVSNFTVALLDEAVAVSPEPLACNQVEY
HPYLDQTKVREACARHGIALVAYSPIAKGKIKNDPTLTRIGRAHGKTTAQICLRWLVQQNVVAIPRTSKIERLSENLDVF
DFELSGQDMADIFAMGSPEGRMTNFAFAPNWD

Sequences:

>Translated_272_residues
MLMIEANGARIPALGLGTWELRGRTCTRIVEQALTLGYRHIDTAQVYENEHEVGEGLRASRVRRNEIFLTTKVWTTHFAP
NDLERSTKESLVRLRVSEVDLLLLHWPNSHVPLAETLGALAHAKKLGLARHIGVSNFTVALLDEAVAVSPEPLACNQVEY
HPYLDQTKVREACARHGIALVAYSPIAKGKIKNDPTLTRIGRAHGKTTAQICLRWLVQQNVVAIPRTSKIERLSENLDVF
DFELSGQDMADIFAMGSPEGRMTNFAFAPNWD
>Mature_272_residues
MLMIEANGARIPALGLGTWELRGRTCTRIVEQALTLGYRHIDTAQVYENEHEVGEGLRASRVRRNEIFLTTKVWTTHFAP
NDLERSTKESLVRLRVSEVDLLLLHWPNSHVPLAETLGALAHAKKLGLARHIGVSNFTVALLDEAVAVSPEPLACNQVEY
HPYLDQTKVREACARHGIALVAYSPIAKGKIKNDPTLTRIGRAHGKTTAQICLRWLVQQNVVAIPRTSKIERLSENLDVF
DFELSGQDMADIFAMGSPEGRMTNFAFAPNWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI4502049, Length=284, Percent_Identity=36.2676056338028, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=33.9041095890411, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI24497577, Length=282, Percent_Identity=32.9787234042553, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI5174391, Length=282, Percent_Identity=32.9787234042553, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI223468663, Length=287, Percent_Identity=33.7979094076655, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI24497585, Length=284, Percent_Identity=33.8028169014084, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=31.864406779661, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI310109922, Length=289, Percent_Identity=33.2179930795848, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI24497583, Length=308, Percent_Identity=31.8181818181818, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI45446745, Length=308, Percent_Identity=32.4675324675325, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4503285, Length=308, Percent_Identity=32.4675324675325, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI310109920, Length=308, Percent_Identity=32.1428571428571, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=33.4545454545455, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5453543, Length=307, Percent_Identity=30.9446254071661, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI300116271, Length=271, Percent_Identity=32.4723247232472, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=32.6996197718631, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI310109924, Length=169, Percent_Identity=33.7278106508876, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI27436969, Length=224, Percent_Identity=29.4642857142857, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4504825, Length=224, Percent_Identity=29.4642857142857, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI27436964, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27436966, Length=239, Percent_Identity=28.4518828451883, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27436962, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI207028673, Length=125, Percent_Identity=34.4, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310109926, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27436971, Length=209, Percent_Identity=28.2296650717703, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=41.1320754716981, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI87082198, Length=254, Percent_Identity=34.6456692913386, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788081, Length=278, Percent_Identity=31.294964028777, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787674, Length=261, Percent_Identity=27.5862068965517, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081735, Length=287, Percent_Identity=26.8292682926829, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789375, Length=308, Percent_Identity=24.6753246753247, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI48994888, Length=199, Percent_Identity=31.1557788944724, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=283, Percent_Identity=39.2226148409894, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17537075, Length=283, Percent_Identity=38.5159010600707, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17566692, Length=278, Percent_Identity=32.7338129496403, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=32.8358208955224, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17537079, Length=288, Percent_Identity=30.9027777777778, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17550248, Length=279, Percent_Identity=31.8996415770609, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17564128, Length=285, Percent_Identity=32.6315789473684, Blast_Score=143, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17561298, Length=266, Percent_Identity=29.6992481203008, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=250, Percent_Identity=33.6, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=32.0754716981132, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=28.8973384030418, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17562292, Length=254, Percent_Identity=29.1338582677165, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321896, Length=301, Percent_Identity=32.2259136212625, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=32.4324324324324, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=30.9803921568627, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320576, Length=267, Percent_Identity=32.9588014981273, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6319625, Length=271, Percent_Identity=31.3653136531365, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320079, Length=277, Percent_Identity=31.7689530685921, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI20129731, Length=273, Percent_Identity=39.1941391941392, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24663317, Length=276, Percent_Identity=36.231884057971, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24644950, Length=308, Percent_Identity=34.4155844155844, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21356425, Length=299, Percent_Identity=32.7759197324415, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24657054, Length=276, Percent_Identity=32.9710144927536, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221378297, Length=325, Percent_Identity=33.2307692307692, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45553081, Length=277, Percent_Identity=34.2960288808664, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24662789, Length=283, Percent_Identity=30.7420494699647, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24662781, Length=272, Percent_Identity=33.8235294117647, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24662785, Length=272, Percent_Identity=33.4558823529412, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281366140, Length=248, Percent_Identity=33.0645161290323, Blast_Score=130, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 30353; Mature: 30353

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMIEANGARIPALGLGTWELRGRTCTRIVEQALTLGYRHIDTAQVYENEHEVGEGLRAS
CEEEECCCCCCCEECCCCEEECCCHHHHHHHHHHHHCHHCCCHHHHHCCHHHHHCCHHHH
RVRRNEIFLTTKVWTTHFAPNDLERSTKESLVRLRVSEVDLLLLHWPNSHVPLAETLGAL
HEECCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
AHAKKLGLARHIGVSNFTVALLDEAVAVSPEPLACNQVEYHPYLDQTKVREACARHGIAL
HHHHHHHHHHHCCCCCEEHEEHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCEEE
VAYSPIAKGKIKNDPTLTRIGRAHGKTTAQICLRWLVQQNVVAIPRTSKIERLSENLDVF
EEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCCEE
DFELSGQDMADIFAMGSPEGRMTNFAFAPNWD
EEEECCCCHHHHHCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLMIEANGARIPALGLGTWELRGRTCTRIVEQALTLGYRHIDTAQVYENEHEVGEGLRAS
CEEEECCCCCCCEECCCCEEECCCHHHHHHHHHHHHCHHCCCHHHHHCCHHHHHCCHHHH
RVRRNEIFLTTKVWTTHFAPNDLERSTKESLVRLRVSEVDLLLLHWPNSHVPLAETLGAL
HEECCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
AHAKKLGLARHIGVSNFTVALLDEAVAVSPEPLACNQVEYHPYLDQTKVREACARHGIAL
HHHHHHHHHHHCCCCCEEHEEHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCEEE
VAYSPIAKGKIKNDPTLTRIGRAHGKTTAQICLRWLVQQNVVAIPRTSKIERLSENLDVF
EEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCCEE
DFELSGQDMADIFAMGSPEGRMTNFAFAPNWD
EEEECCCCHHHHHCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]