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The map label for this gene is dacF [H]

Identifier: 146341457

GI number: 146341457

Start: 4747308

End: 4749068

Strand: Direct

Name: dacF [H]

Synonym: BRADO4549

Alternate gene names: 146341457

Gene position: 4747308-4749068 (Clockwise)

Preceding gene: 146341453

Following gene: 146341462

Centisome position: 63.67

GC content: 69.51

Gene sequence:

>1761_bases
ATGCTTCGCAAAAACTTGGCTTCCTCGCGCCTGCTGCGGGTTGGCGTCTCAGGGCTGATCACCCTTGCGGTGGCGGCAGT
GGTCACCGTCGACAGCGCGGAAGCGCGCCGGCACCATCGGCATCACGCGCGTCACTCCCCCCACAATGACGAGGGCAGCT
ACAGCCCGGCGTTCGCCTCGATCGTCGTCGACGGCAATTCGGGCGCGACCCTGACTGCGACCAATCCGGACGCGTTGCGG
CACCCGGCGTCGCTCACCAAGATCATGACGCTTTATCTGCTCTTCGAGCGCCTCGACTCCGGCAAGATGAAGCTCGACAC
CGAGATGCCGGTGTCGGAGCACGCCTCCGAGCAGTCGCCGACCAAGCTCGGCCTGCGTCCGGGCCAGACCATCCGCGTCG
AGGATGCGATCAAAGGCCTAGTGACCCGTTCGGCCAACGATGCCGCCGTGGTCATCGCGGAGGCGATCGGCGGCGACGAG
GACGAGTTCGCCAAGATGATGACGCGCAAGGCGCGCTCGCTCGGCATGAGCCGCACCACCTATGTCAACGCATCGGGCCT
GCCGGCCGACGAGCAGATCACGACGGCGCGCGACCAGTCCATCCTCGGCCGCGCCATCCAGGACCGCTTCCCGCGCTACT
ATCGCTATTTCTCGACCGTAGTGTTCAATTATCGCGGCCAGTCGATCCGCAACCACAATCACCTGCTCGGCAATGTCGAG
GGCATCGACGGCATCAAGACCGGCTATACCCGCGCCTCCGGCTTCAACATCGTGACCTCGCTGCGCCGCGGCAACCGCCA
TCTGGTCGGCGTCGTGCTCGGCGGCCGCAGCGGCGGCTCGCGCGACGCCATCATGCGCAACCTGCTTGCAGAGAACCTCG
AGAAGGGCGCGACCAAGCGCACGGTTGCGGCGGTCGGCGAGCGCGGCAACTCCGACGCCGGCGACGACCAGAGCACGGTG
CAGCTCGCCTCGGCCGCGCCCGAGACCCCGGCTCCGAGCCGGGCCGCAAAATTCATTTCGACGCTTTCGGAGGCCACCGC
CGCCTTGCCGCCGGCTGCGCCCAAGGAAGCCAAGGTCGAGCCGGCACCGATGACCAACGGCGTCATCCAGAATCAGCCGA
TCTCGCTCGTTCCCGGCTCGACCGAGCCGATGAAGCCGGTGAAGGTGCGGACGGTTCAGGTCAAGGCCGGCGCCGTCAAG
CTCGCCACCGCCGCCCCGCAGCCGGTGGCGGCCGCCCCGGCCCCCGCGCCGTTCGCGCAGCGCGTCGAGGCGATCGAGCC
GGCACCGCCGATCGCCCGCGCCGAAGTCGCCGCGCGGCCGGACATGCCGCCGCAGCCGCCGGGCCACGGCACCGGCAACG
GCATTCTCGGCGTTTTGCCGGCCTCCAGCCTGCCGCCGGCGTCGTCCCAGGCGATGGCCTATGCCGCGCCCCCGGCCCAG
CCGGCTCCGCCGGCCCCGGTCCAGATGGCGTCCGCCAACCCCGCTCCGGACGCGCCCGCCCGCGCCGCCGTCGCGCGCAG
CGGCTGGATCATCCAGGTCGGCGCACTCGACAGCGAGGCCGAGGCGCAGAAGCGCATCGATGCGGCGCGCAGCTCCGCGC
AGGGCCTCTTGAGCAAGGCCGATCCGTTCACCGAGACGGTCTCGAAGGGCGACAAGACGCTGATCCGCGCCCGCTTCGCA
GGTCTCGATCGCGATCAGGCCGAAGCGGTCTGCAAGAAGCTCAAGCGCGCCGACATCTCCTGCATGACCGTACACAACTA
A

Upstream 100 bases:

>100_bases
GGCGCACACTGGAGGTCCGACAGGCAAAGATCACGGGGACGTTTTGGCACAATTTCGCCTCAACTTCCGGTGATCACGTC
AGAAATTGGACGGGGATTTC

Downstream 100 bases:

>100_bases
CTCACCGGCATCGCCGCATGTGAGGCCCGCGCGACACACGCGCGGGCCTTTTTCTTTGCCTGCGCGCGACTCCGCAACAA
GCGGGCGCACGCAAACCACT

Product: putative D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MLRKNLASSRLLRVGVSGLITLAVAAVVTVDSAEARRHHRHHARHSPHNDEGSYSPAFASIVVDGNSGATLTATNPDALR
HPASLTKIMTLYLLFERLDSGKMKLDTEMPVSEHASEQSPTKLGLRPGQTIRVEDAIKGLVTRSANDAAVVIAEAIGGDE
DEFAKMMTRKARSLGMSRTTYVNASGLPADEQITTARDQSILGRAIQDRFPRYYRYFSTVVFNYRGQSIRNHNHLLGNVE
GIDGIKTGYTRASGFNIVTSLRRGNRHLVGVVLGGRSGGSRDAIMRNLLAENLEKGATKRTVAAVGERGNSDAGDDQSTV
QLASAAPETPAPSRAAKFISTLSEATAALPPAAPKEAKVEPAPMTNGVIQNQPISLVPGSTEPMKPVKVRTVQVKAGAVK
LATAAPQPVAAAPAPAPFAQRVEAIEPAPPIARAEVAARPDMPPQPPGHGTGNGILGVLPASSLPPASSQAMAYAAPPAQ
PAPPAPVQMASANPAPDAPARAAVARSGWIIQVGALDSEAEAQKRIDAARSSAQGLLSKADPFTETVSKGDKTLIRARFA
GLDRDQAEAVCKKLKRADISCMTVHN

Sequences:

>Translated_586_residues
MLRKNLASSRLLRVGVSGLITLAVAAVVTVDSAEARRHHRHHARHSPHNDEGSYSPAFASIVVDGNSGATLTATNPDALR
HPASLTKIMTLYLLFERLDSGKMKLDTEMPVSEHASEQSPTKLGLRPGQTIRVEDAIKGLVTRSANDAAVVIAEAIGGDE
DEFAKMMTRKARSLGMSRTTYVNASGLPADEQITTARDQSILGRAIQDRFPRYYRYFSTVVFNYRGQSIRNHNHLLGNVE
GIDGIKTGYTRASGFNIVTSLRRGNRHLVGVVLGGRSGGSRDAIMRNLLAENLEKGATKRTVAAVGERGNSDAGDDQSTV
QLASAAPETPAPSRAAKFISTLSEATAALPPAAPKEAKVEPAPMTNGVIQNQPISLVPGSTEPMKPVKVRTVQVKAGAVK
LATAAPQPVAAAPAPAPFAQRVEAIEPAPPIARAEVAARPDMPPQPPGHGTGNGILGVLPASSLPPASSQAMAYAAPPAQ
PAPPAPVQMASANPAPDAPARAAVARSGWIIQVGALDSEAEAQKRIDAARSSAQGLLSKADPFTETVSKGDKTLIRARFA
GLDRDQAEAVCKKLKRADISCMTVHN
>Mature_586_residues
MLRKNLASSRLLRVGVSGLITLAVAAVVTVDSAEARRHHRHHARHSPHNDEGSYSPAFASIVVDGNSGATLTATNPDALR
HPASLTKIMTLYLLFERLDSGKMKLDTEMPVSEHASEQSPTKLGLRPGQTIRVEDAIKGLVTRSANDAAVVIAEAIGGDE
DEFAKMMTRKARSLGMSRTTYVNASGLPADEQITTARDQSILGRAIQDRFPRYYRYFSTVVFNYRGQSIRNHNHLLGNVE
GIDGIKTGYTRASGFNIVTSLRRGNRHLVGVVLGGRSGGSRDAIMRNLLAENLEKGATKRTVAAVGERGNSDAGDDQSTV
QLASAAPETPAPSRAAKFISTLSEATAALPPAAPKEAKVEPAPMTNGVIQNQPISLVPGSTEPMKPVKVRTVQVKAGAVK
LATAAPQPVAAAPAPAPFAQRVEAIEPAPPIARAEVAARPDMPPQPPGHGTGNGILGVLPASSLPPASSQAMAYAAPPAQ
PAPPAPVQMASANPAPDAPARAAVARSGWIIQVGALDSEAEAQKRIDAARSSAQGLLSKADPFTETVSKGDKTLIRARFA
GLDRDQAEAVCKKLKRADISCMTVHN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786851, Length=250, Percent_Identity=34, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787062, Length=226, Percent_Identity=34.5132743362832, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87082054, Length=226, Percent_Identity=28.3185840707965, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 61634; Mature: 61634

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKNLASSRLLRVGVSGLITLAVAAVVTVDSAEARRHHRHHARHSPHNDEGSYSPAFAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IVVDGNSGATLTATNPDALRHPASLTKIMTLYLLFERLDSGKMKLDTEMPVSEHASEQSP
EEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCC
TKLGLRPGQTIRVEDAIKGLVTRSANDAAVVIAEAIGGDEDEFAKMMTRKARSLGMSRTT
CEECCCCCCEEEHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEE
YVNASGLPADEQITTARDQSILGRAIQDRFPRYYRYFSTVVFNYRGQSIRNHNHLLGNVE
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
GIDGIKTGYTRASGFNIVTSLRRGNRHLVGVVLGGRSGGSRDAIMRNLLAENLEKGATKR
CCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
TVAAVGERGNSDAGDDQSTVQLASAAPETPAPSRAAKFISTLSEATAALPPAAPKEAKVE
HHHHHCCCCCCCCCCCHHHEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAPMTNGVIQNQPISLVPGSTEPMKPVKVRTVQVKAGAVKLATAAPQPVAAAPAPAPFAQ
CCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCCCCCHHHH
RVEAIEPAPPIARAEVAARPDMPPQPPGHGTGNGILGVLPASSLPPASSQAMAYAAPPAQ
HHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCC
PAPPAPVQMASANPAPDAPARAAVARSGWIIQVGALDSEAEAQKRIDAARSSAQGLLSKA
CCCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
DPFTETVSKGDKTLIRARFAGLDRDQAEAVCKKLKRADISCMTVHN
CCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MLRKNLASSRLLRVGVSGLITLAVAAVVTVDSAEARRHHRHHARHSPHNDEGSYSPAFAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IVVDGNSGATLTATNPDALRHPASLTKIMTLYLLFERLDSGKMKLDTEMPVSEHASEQSP
EEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCC
TKLGLRPGQTIRVEDAIKGLVTRSANDAAVVIAEAIGGDEDEFAKMMTRKARSLGMSRTT
CEECCCCCCEEEHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEE
YVNASGLPADEQITTARDQSILGRAIQDRFPRYYRYFSTVVFNYRGQSIRNHNHLLGNVE
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
GIDGIKTGYTRASGFNIVTSLRRGNRHLVGVVLGGRSGGSRDAIMRNLLAENLEKGATKR
CCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
TVAAVGERGNSDAGDDQSTVQLASAAPETPAPSRAAKFISTLSEATAALPPAAPKEAKVE
HHHHHCCCCCCCCCCCHHHEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAPMTNGVIQNQPISLVPGSTEPMKPVKVRTVQVKAGAVKLATAAPQPVAAAPAPAPFAQ
CCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCCCCCHHHH
RVEAIEPAPPIARAEVAARPDMPPQPPGHGTGNGILGVLPASSLPPASSQAMAYAAPPAQ
HHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCC
PAPPAPVQMASANPAPDAPARAAVARSGWIIQVGALDSEAEAQKRIDAARSSAQGLLSKA
CCCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
DPFTETVSKGDKTLIRARFAGLDRDQAEAVCKKLKRADISCMTVHN
CCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]