Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 146341396

GI number: 146341396

Start: 4685325

End: 4686842

Strand: Reverse

Name: gatB [H]

Synonym: BRADO4486

Alternate gene names: 146341396

Gene position: 4686842-4685325 (Counterclockwise)

Preceding gene: 146341398

Following gene: 146341395

Centisome position: 62.86

GC content: 65.74

Gene sequence:

>1518_bases
ATGCAACCGACACTTTGCCTGGACACCACTGCCATGAACGCACCCGCCACACCGCACAAGCTGATCAAGGGCGCCACCGG
CGACTGGGAGACCGTGATCGGCATGGAGATCCATGCCCAGGTCACCTCGAAGTCGAAACTGTTCTCGGGCGCCTCGACGG
CCTTCGGCGGCGATCCCAATACCCACGTCTCGCTGGTCGACGCCGCGATGCCGGGCATGCTGCCGGTCATCAACGAGGAG
TGCGTGCGCCAGGCCGTGCGGACCGGGCTCGGGCTGAACGCCAAGATCAACCTGCGCTCGGTGTTCGACCGCAAGAACTA
TTTCTACCCCGACCTGCCGCAAGGCTACCAGATCAGCCAGTACAAGGACCCGATCGTGGGCGAGGGCGAGGTCACGGTCG
AGCTCGACGGCGGCAAGACCGCGACCATCGGCATCGAGCGTCTGCACCTCGAGCAGGATCCGGGCAAGATGCTGCACGAC
CAGTCGCCGTCGCTGTCCTATATCGACTTCAACCGCTGCGGCGTGGCGCTGATGGAGATCGTCTCCAAGCCAGACATCCG
CGACGCCGAGCAGGCCAAGGCCTACGTGACCAAGCTGCGCTCGATCATGCGCTATCTCGGCACCTGCGACGGCGACATGG
AGAAGGGCTCACTGCGCGCCGACGTCAACGTCTCCGTCCGCAAGCCGGGCGGGCCGCTCGGCACCCGCTGCGAGATCAAG
AACATCAACTCCATCAGCTTCATCGGCCAGGCGGTGGAGGCCGAGGCGCGGCGCCAGATCGAGATTCTCGAGGACGGCGG
CGTCATCGAGCAGGAGACCCGGCGGTTCGATCCCGACAAGGGCGAGACGCGCTCGATGCGCTCCAAGGAGGAGGCGCATG
ACTACCGTTACTTCCCGGACCCGGACCTGTTGCCGCTGGAGTTCGACCAGGCTTTCGTCGACGAGCTGAAGGCGGCGCTG
CCGGAGCTGCCGGACCAGAAGAAGGCGCGCTTCATCGCCGATTTCGGTCTCTCGCCGTACGACGCCTCGGTACTGGTCGC
CGAACGCGAGAGCGCGGCGTTCTACGAAGCCGTGCTGGACGCGCTGTCCGAGCGGGTGCGCGACGGCAAGCTCGCCGCCA
ACTGGGTGATCAACGAGCTGTTCGGGCGGCTCAACAAAGAGGGCCGCGACATCGCATTCTCGCCGGTGTCGGCCGCGCAG
CTCGCGTCCATCGTCGCGCTGATCGGCGAGGGCATCATCTCCGGCAAGATCGCCAAGGATCTGTTCGAGATCGTCTGGAG
CGAGGGCGGCGATCCGCGCGAGCTGGTCGAGAGCCGCGGCATGAAGCAGGTCACCGACCTCGGCGCGATCGAGAAGGTGG
TCGACGAGATCATCGCCGCCAATCCGGACAAGGTCGCGCAGGCCAAGGCGAAGCCGCAGCTCGCCGGCTGGTTCGTCGGT
CAGGTGATGAAGTCGTCGGGCGGCAAGGCCAACCCGCAGGCGGTCAACGATCTCCTGAAGAGCAAGCTCGGCATCTGA

Upstream 100 bases:

>100_bases
GCACTCGGATTGTCTCGCATTCACAGGCGGAAGGCGCCGCTTGCGGGCCGCAGCGGAGGCTCGTCCGGCTTGACTGCCGG
GTGACGTTGCGCGAAGCCGG

Downstream 100 bases:

>100_bases
AACGACGCTCGCGGATCTTCGTTCCGACGGCGCGCACGAAGGTGCGCGCCGTTTTGCTTGGCGCTCGCGTTCGGCGAATC
GCCGCGCGCGATTCGCAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MQPTLCLDTTAMNAPATPHKLIKGATGDWETVIGMEIHAQVTSKSKLFSGASTAFGGDPNTHVSLVDAAMPGMLPVINEE
CVRQAVRTGLGLNAKINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVTVELDGGKTATIGIERLHLEQDPGKMLHD
QSPSLSYIDFNRCGVALMEIVSKPDIRDAEQAKAYVTKLRSIMRYLGTCDGDMEKGSLRADVNVSVRKPGGPLGTRCEIK
NINSISFIGQAVEAEARRQIEILEDGGVIEQETRRFDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDELKAAL
PELPDQKKARFIADFGLSPYDASVLVAERESAAFYEAVLDALSERVRDGKLAANWVINELFGRLNKEGRDIAFSPVSAAQ
LASIVALIGEGIISGKIAKDLFEIVWSEGGDPRELVESRGMKQVTDLGAIEKVVDEIIAANPDKVAQAKAKPQLAGWFVG
QVMKSSGGKANPQAVNDLLKSKLGI

Sequences:

>Translated_505_residues
MQPTLCLDTTAMNAPATPHKLIKGATGDWETVIGMEIHAQVTSKSKLFSGASTAFGGDPNTHVSLVDAAMPGMLPVINEE
CVRQAVRTGLGLNAKINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVTVELDGGKTATIGIERLHLEQDPGKMLHD
QSPSLSYIDFNRCGVALMEIVSKPDIRDAEQAKAYVTKLRSIMRYLGTCDGDMEKGSLRADVNVSVRKPGGPLGTRCEIK
NINSISFIGQAVEAEARRQIEILEDGGVIEQETRRFDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDELKAAL
PELPDQKKARFIADFGLSPYDASVLVAERESAAFYEAVLDALSERVRDGKLAANWVINELFGRLNKEGRDIAFSPVSAAQ
LASIVALIGEGIISGKIAKDLFEIVWSEGGDPRELVESRGMKQVTDLGAIEKVVDEIIAANPDKVAQAKAKPQLAGWFVG
QVMKSSGGKANPQAVNDLLKSKLGI
>Mature_505_residues
MQPTLCLDTTAMNAPATPHKLIKGATGDWETVIGMEIHAQVTSKSKLFSGASTAFGGDPNTHVSLVDAAMPGMLPVINEE
CVRQAVRTGLGLNAKINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVTVELDGGKTATIGIERLHLEQDPGKMLHD
QSPSLSYIDFNRCGVALMEIVSKPDIRDAEQAKAYVTKLRSIMRYLGTCDGDMEKGSLRADVNVSVRKPGGPLGTRCEIK
NINSISFIGQAVEAEARRQIEILEDGGVIEQETRRFDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDELKAAL
PELPDQKKARFIADFGLSPYDASVLVAERESAAFYEAVLDALSERVRDGKLAANWVINELFGRLNKEGRDIAFSPVSAAQ
LASIVALIGEGIISGKIAKDLFEIVWSEGGDPRELVESRGMKQVTDLGAIEKVVDEIIAANPDKVAQAKAKPQLAGWFVG
QVMKSSGGKANPQAVNDLLKSKLGI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=337, Percent_Identity=39.7626112759644, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=32.0537428023033, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=36.969696969697, Blast_Score=318, Evalue=6e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 55108; Mature: 55108

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTLCLDTTAMNAPATPHKLIKGATGDWETVIGMEIHAQVTSKSKLFSGASTAFGGDPN
CCCCEEEECCCCCCCCCHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCCCCCCCC
THVSLVDAAMPGMLPVINEECVRQAVRTGLGLNAKINLRSVFDRKNYFYPDLPQGYQISQ
CEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCC
YKDPIVGEGEVTVELDGGKTATIGIERLHLEQDPGKMLHDQSPSLSYIDFNRCGVALMEI
CCCCCCCCCEEEEEECCCCEEEEEEEEEEECCCCCHHHCCCCCCCCEEECHHHHHHHHHH
VSKPDIRDAEQAKAYVTKLRSIMRYLGTCDGDMEKGSLRADVNVSVRKPGGPLGTRCEIK
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEC
NINSISFIGQAVEAEARRQIEILEDGGVIEQETRRFDPDKGETRSMRSKEEAHDYRYFPD
CCCHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PDLLPLEFDQAFVDELKAALPELPDQKKARFIADFGLSPYDASVLVAERESAAFYEAVLD
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHH
ALSERVRDGKLAANWVINELFGRLNKEGRDIAFSPVSAAQLASIVALIGEGIISGKIAKD
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHH
LFEIVWSEGGDPRELVESRGMKQVTDLGAIEKVVDEIIAANPDKVAQAKAKPQLAGWFVG
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
QVMKSSGGKANPQAVNDLLKSKLGI
HHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPTLCLDTTAMNAPATPHKLIKGATGDWETVIGMEIHAQVTSKSKLFSGASTAFGGDPN
CCCCEEEECCCCCCCCCHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCCCCCCCC
THVSLVDAAMPGMLPVINEECVRQAVRTGLGLNAKINLRSVFDRKNYFYPDLPQGYQISQ
CEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCC
YKDPIVGEGEVTVELDGGKTATIGIERLHLEQDPGKMLHDQSPSLSYIDFNRCGVALMEI
CCCCCCCCCEEEEEECCCCEEEEEEEEEEECCCCCHHHCCCCCCCCEEECHHHHHHHHHH
VSKPDIRDAEQAKAYVTKLRSIMRYLGTCDGDMEKGSLRADVNVSVRKPGGPLGTRCEIK
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEC
NINSISFIGQAVEAEARRQIEILEDGGVIEQETRRFDPDKGETRSMRSKEEAHDYRYFPD
CCCHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PDLLPLEFDQAFVDELKAALPELPDQKKARFIADFGLSPYDASVLVAERESAAFYEAVLD
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHH
ALSERVRDGKLAANWVINELFGRLNKEGRDIAFSPVSAAQLASIVALIGEGIISGKIAKD
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHH
LFEIVWSEGGDPRELVESRGMKQVTDLGAIEKVVDEIIAANPDKVAQAKAKPQLAGWFVG
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
QVMKSSGGKANPQAVNDLLKSKLGI
HHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA