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The map label for this gene is glyA [H]

Identifier: 146341346

GI number: 146341346

Start: 4633100

End: 4634401

Strand: Reverse

Name: glyA [H]

Synonym: BRADO4430

Alternate gene names: 146341346

Gene position: 4634401-4633100 (Counterclockwise)

Preceding gene: 146341347

Following gene: 146341345

Centisome position: 62.15

GC content: 65.44

Gene sequence:

>1302_bases
ATGACCGCTTCTGCCAAGCCCGTATCGTCAGTCGATTCCTTCTTTTCCGCCACCCTGGCCGAGGCTGATCCCGAGATCGC
CGCCGCCATCAGGGGCGAACTCGGTCGGCAACGGCACGAAATCGAGCTGATCGCTTCGGAAAACATCGTCAGCCGGGCGG
TCCTGGAGGCCCAGGGCTCGGTGATGACCAACAAGTATGCCGAGGGCTATCCGGGCGCTCGGTATTACGGCGGCTGCGAA
TGGGTCGACGTCGCCGAGAACCTCGCGATCGACCGCGCCAAGAAGCTGTTCGGCGCCAATTTCGCCAACGTCCAGCCGAA
CTCGGGCAGCCAGATGAATCAGGCCGTGTTCCTGGCGCTGCTGCAGCCGGGCGACACCTTCATGGGCCTCGACCTCGCCG
CGGGCGGTCATCTCACCCACGGTTCGCCGGTCAATATGTCCGGCAAGTGGTTCAAGGCCGCGCATTACACCGTGCGCCGC
GACGACCACCTCATCGACATGGATGCGGTGGCCAAGCAGGCCGAAGAGGTGAAGCCGAAGCTGATCATCGCCGGCGGCAG
TGCCTATTCGCGCCCGTGGGACTTCAAGCGCTTCCGCGAGATCGCGGACAGCGTCGGCGCCTATCTGCTGGTCGACATGG
CCCACTTCGCCGGCCTCGTCGCTGGTGGCGTGCATGCCTCGCCGGTGCCTTACGCTCACATCACCACCACCACGACCCAT
AAGTCGCTGCGCGGTCCGCGCGGCGGCCTGATGCTCTGGAATGACGAGCAGTTCACCAAGAAGTTCAACTCGGCGATCTT
CCCGGGACTTCAGGGCGGTCCGCTCATGCACGTGATCGCGGCCAAGGCGGTGGCGTTCGCCGAGGCGCTGCGGCCGGAGT
TCAAGGCCTACGCGAAGAACGTCGTGGAGAATGCGAAGGCGCTGGCGGAATCGCTGCGCGCGCAAGGCTTCGACATCGTC
TCCGGCGGCACCGACAACCACCTGATGCTGGTCGATCTTCGGCCCAAGGGCCTGAAGGGCAACGTGTCCGAGAAGGCGCT
CGTCCGCGCCGCCATCACCTGCAACAAGAACGGCATTCCGTTCGACCCCGAGAAGCCGTTCGTCACCTCCGGCCTGCGCC
TGGGCACGCCGGCAGCGACCACCCGCGGCTTCGGCGTCGCTGAATTCCAGCAGGTCGGCAGTCTGATCGCCGAGGTGCTG
AACGCGATCGCGCAGGGCCCTGACGGCAGCGCGCCGCTGGTCGAAGCCGCGGTCAAGGAGAAGGTGAAGGCGCTGACCGA
CCGGTTCCCGATCTATCAGTAA

Upstream 100 bases:

>100_bases
CCGCCGCCCGTATGGTAGACGGCAGGGGTCTTTTCCGACCGCCTTCACTGAACCGACCGCAGGCTCCTTAAGGCCTTGCG
GACGTGGAGATATTCAGCCG

Downstream 100 bases:

>100_bases
GGCGTAAGGCGACATGCGCTGCCCGAGCTGCAACAGCCTCGATACGCAGGTGAAGGACTCCCGCCCCACCGAGGATTCCT
CGGTGATCCGGCGGCGGCGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTASAKPVSSVDSFFSATLAEADPEIAAAIRGELGRQRHEIELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCE
WVDVAENLAIDRAKKLFGANFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKAAHYTVRR
DDHLIDMDAVAKQAEEVKPKLIIAGGSAYSRPWDFKRFREIADSVGAYLLVDMAHFAGLVAGGVHASPVPYAHITTTTTH
KSLRGPRGGLMLWNDEQFTKKFNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKAYAKNVVENAKALAESLRAQGFDIV
SGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGSLIAEVL
NAIAQGPDGSAPLVEAAVKEKVKALTDRFPIYQ

Sequences:

>Translated_433_residues
MTASAKPVSSVDSFFSATLAEADPEIAAAIRGELGRQRHEIELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCE
WVDVAENLAIDRAKKLFGANFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKAAHYTVRR
DDHLIDMDAVAKQAEEVKPKLIIAGGSAYSRPWDFKRFREIADSVGAYLLVDMAHFAGLVAGGVHASPVPYAHITTTTTH
KSLRGPRGGLMLWNDEQFTKKFNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKAYAKNVVENAKALAESLRAQGFDIV
SGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGSLIAEVL
NAIAQGPDGSAPLVEAAVKEKVKALTDRFPIYQ
>Mature_432_residues
TASAKPVSSVDSFFSATLAEADPEIAAAIRGELGRQRHEIELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCEW
VDVAENLAIDRAKKLFGANFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKAAHYTVRRD
DHLIDMDAVAKQAEEVKPKLIIAGGSAYSRPWDFKRFREIADSVGAYLLVDMAHFAGLVAGGVHASPVPYAHITTTTTHK
SLRGPRGGLMLWNDEQFTKKFNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKAYAKNVVENAKALAESLRAQGFDIVS
GGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGSLIAEVLN
AIAQGPDGSAPLVEAAVKEKVKALTDRFPIYQ

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=47.6658476658477, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=47.6658476658477, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=47.6658476658477, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=47.6658476658477, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI261862346, Length=407, Percent_Identity=46.9287469287469, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI22547186, Length=411, Percent_Identity=47.6885644768856, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI22547189, Length=397, Percent_Identity=45.3400503778337, Blast_Score=301, Evalue=6e-82,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.5539568345324, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=47.5124378109453, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=47.5124378109453, Blast_Score=354, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=45.6310679611651, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=422, Percent_Identity=43.3649289099526, Blast_Score=332, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640005, Length=414, Percent_Identity=45.6521739130435, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI221329721, Length=414, Percent_Identity=45.6521739130435, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46306; Mature: 46175

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAKPVSSVDSFFSATLAEADPEIAAAIRGELGRQRHEIELIASENIVSRAVLEAQGS
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCC
VMTNKYAEGYPGARYYGGCEWVDVAENLAIDRAKKLFGANFANVQPNSGSQMNQAVFLAL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEE
LQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKAAHYTVRRDDHLIDMDAVAKQAEEVKPK
ECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCEECHHHHHHHHHHCCCE
LIIAGGSAYSRPWDFKRFREIADSVGAYLLVDMAHFAGLVAGGVHASPVPYAHITTTTTH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
KSLRGPRGGLMLWNDEQFTKKFNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKAYAKN
HHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
VVENAKALAESLRAQGFDIVSGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIP
HHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHEEECCCCCC
FDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGSLIAEVLNAIAQGPDGSAPLVEAAVKE
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KVKALTDRFPIYQ
HHHHHHHHCCCCC
>Mature Secondary Structure 
TASAKPVSSVDSFFSATLAEADPEIAAAIRGELGRQRHEIELIASENIVSRAVLEAQGS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCC
VMTNKYAEGYPGARYYGGCEWVDVAENLAIDRAKKLFGANFANVQPNSGSQMNQAVFLAL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEE
LQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKAAHYTVRRDDHLIDMDAVAKQAEEVKPK
ECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCEECHHHHHHHHHHCCCE
LIIAGGSAYSRPWDFKRFREIADSVGAYLLVDMAHFAGLVAGGVHASPVPYAHITTTTTH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
KSLRGPRGGLMLWNDEQFTKKFNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKAYAKN
HHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
VVENAKALAESLRAQGFDIVSGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIP
HHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHEEECCCCCC
FDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGSLIAEVLNAIAQGPDGSAPLVEAAVKE
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KVKALTDRFPIYQ
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA