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The map label for this gene is acrA [H]

Identifier: 146341330

GI number: 146341330

Start: 4616439

End: 4617590

Strand: Reverse

Name: acrA [H]

Synonym: BRADO4412

Alternate gene names: 146341330

Gene position: 4617590-4616439 (Counterclockwise)

Preceding gene: 146341331

Following gene: 146341329

Centisome position: 61.93

GC content: 63.28

Gene sequence:

>1152_bases
ATGCAGATGAGCGGGCTGAAAATCTTGAGTTCTTCTGTCTGGCTGCTGATCCTTGCCCTCCAGGCGACGGGCATAGATGC
GTCGCACGCCCAGATGGCTCCTCCTGCGGTGGATGTTGCAAAACCTCTGCAGCGCACGATCGATCGCTGGGACGAGTACA
CCGGCCGGTTCGAGGCTGTTCAGCAGGTGGAGGTCCGGCCTCGGGTCTCTGGATTTCTGACGTCCATTGCGTTCAAGGAT
GGAGAACTCGTCAGAGCAGGCGAGCTCCTGTTTACAATCGATCCGCGTCCGTACGAAATCAGCGTCGAGAGCGCACAGGC
GGAAATCGCCAAGGCCAAAGCCCAGGTCGCCCAGGCGGCGACCGATGTGTCCCGCGCCCAGCAGCTCATCGGCAGTCAGG
CGATCACTCAGCGTGACGTTGACGCCAGGAAGGCAACGCTGGACGTCGCCGAAGGGCAGCTTCTGGCGGCGCAGGCCTCG
CTGCGAGCGGCCCAGCTCAATCTCGAATGGACGCGCGTGACAGCTCCGATCTCCGGCAGGATCTCGGACAGGAAGGTCGA
CGTCGGCAACCTGGTGGATTCCGCGTCCTCCGTTCTGACCAGAATCGTTGCTCTGGATCCGATCCACTTTGTCTTCGAGG
CCTCCGAGTCCGATTACCTGCGCTACTCCCGGCTGGCGGCAGAGCGGAAGCGTGCGTCCTCCCGCGACGCCCCGACGGCG
GTCGACGTCCGTCTGGCTGACGAGGCCGAGTGGACGTGGCATGGCAGTATGAATTTCGTCGACAATCAGCTGGGCGCTCG
AACCGGCACCATTCGCGGACGGGCCGTTTTCGCCAACCCGAATCTGTTTCTCATCCCAGGTGAGTTCGGACGAGCGCGGC
TGTTCGGCGGAAAGGCGGACGTGCTGCTGATACCGGATGCTGCGGTGTTGTCGGATCAGGCGAGCAAGATTGTGCTGGTG
GTGGGCGCGGGTGCGAAGCTCGGTGCCAAACCCGTGAGGCTCGGCCCGATCGCATTAGGGCTTCGGAGCGTATTCGAGGG
GCTTTCGCCAGACGACGTTGTCGTCGTTGGCGGACTCGCCAATCCCGCGGTCCGGCCGGGTGCGGTGGTCACGCCGCACA
ATACGGAGATCGTCGCGCGTAGCGATCGTTAG

Upstream 100 bases:

>100_bases
CGACGCAAGAATGCACCGGATACAGCAAGAAAGTACGCGCGTCGCGCCTGCCCGGGGGAGCAGAATGCGATCGCGGCGCG
GCGTGTGACTCACCGGGACC

Downstream 100 bases:

>100_bases
GATCTGAGCCATGCGGTTCTCGCACTACTTTGTCGATCGGCCGATCTTTGCCGCCGTGCTGTCCATCATCCTCACCATCG
CCGGGGCGATCACGCTGACG

Product: AcrB/AcrD/AcrF family mulitdrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MQMSGLKILSSSVWLLILALQATGIDASHAQMAPPAVDVAKPLQRTIDRWDEYTGRFEAVQQVEVRPRVSGFLTSIAFKD
GELVRAGELLFTIDPRPYEISVESAQAEIAKAKAQVAQAATDVSRAQQLIGSQAITQRDVDARKATLDVAEGQLLAAQAS
LRAAQLNLEWTRVTAPISGRISDRKVDVGNLVDSASSVLTRIVALDPIHFVFEASESDYLRYSRLAAERKRASSRDAPTA
VDVRLADEAEWTWHGSMNFVDNQLGARTGTIRGRAVFANPNLFLIPGEFGRARLFGGKADVLLIPDAAVLSDQASKIVLV
VGAGAKLGAKPVRLGPIALGLRSVFEGLSPDDVVVVGGLANPAVRPGAVVTPHNTEIVARSDR

Sequences:

>Translated_383_residues
MQMSGLKILSSSVWLLILALQATGIDASHAQMAPPAVDVAKPLQRTIDRWDEYTGRFEAVQQVEVRPRVSGFLTSIAFKD
GELVRAGELLFTIDPRPYEISVESAQAEIAKAKAQVAQAATDVSRAQQLIGSQAITQRDVDARKATLDVAEGQLLAAQAS
LRAAQLNLEWTRVTAPISGRISDRKVDVGNLVDSASSVLTRIVALDPIHFVFEASESDYLRYSRLAAERKRASSRDAPTA
VDVRLADEAEWTWHGSMNFVDNQLGARTGTIRGRAVFANPNLFLIPGEFGRARLFGGKADVLLIPDAAVLSDQASKIVLV
VGAGAKLGAKPVRLGPIALGLRSVFEGLSPDDVVVVGGLANPAVRPGAVVTPHNTEIVARSDR
>Mature_383_residues
MQMSGLKILSSSVWLLILALQATGIDASHAQMAPPAVDVAKPLQRTIDRWDEYTGRFEAVQQVEVRPRVSGFLTSIAFKD
GELVRAGELLFTIDPRPYEISVESAQAEIAKAKAQVAQAATDVSRAQQLIGSQAITQRDVDARKATLDVAEGQLLAAQAS
LRAAQLNLEWTRVTAPISGRISDRKVDVGNLVDSASSVLTRIVALDPIHFVFEASESDYLRYSRLAAERKRASSRDAPTA
VDVRLADEAEWTWHGSMNFVDNQLGARTGTIRGRAVFANPNLFLIPGEFGRARLFGGKADVLLIPDAAVLSDQASKIVLV
VGAGAKLGAKPVRLGPIALGLRSVFEGLSPDDVVVVGGLANPAVRPGAVVTPHNTEIVARSDR

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=35.7723577235772, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789665, Length=359, Percent_Identity=33.7047353760446, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=32.4242424242424, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=28.9256198347107, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081951, Length=187, Percent_Identity=31.5508021390374, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789637, Length=155, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081796, Length=143, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790519, Length=198, Percent_Identity=26.2626262626263, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41026; Mature: 41026

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMSGLKILSSSVWLLILALQATGIDASHAQMAPPAVDVAKPLQRTIDRWDEYTGRFEAV
CCCCCHHHHHHHHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
QQVEVRPRVSGFLTSIAFKDGELVRAGELLFTIDPRPYEISVESAQAEIAKAKAQVAQAA
HHHHCCHHHHHHHHHHEECCCCEEEECCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHH
TDVSRAQQLIGSQAITQRDVDARKATLDVAEGQLLAAQASLRAAQLNLEWTRVTAPISGR
HHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEHHHCEEEEEEEEEEEEECCCCCC
ISDRKVDVGNLVDSASSVLTRIVALDPIHFVFEASESDYLRYSRLAAERKRASSRDAPTA
CCCCEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCE
VDVRLADEAEWTWHGSMNFVDNQLGARTGTIRGRAVFANPNLFLIPGEFGRARLFGGKAD
EEEEEECCCCEEECCCCCHHHHHHCCCCCCEECEEEEECCCEEEEECCCCCEEEECCCCC
VLLIPDAAVLSDQASKIVLVVGAGAKLGAKPVRLGPIALGLRSVFEGLSPDDVVVVGGLA
EEECCCHHHHCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHHCCCCCCCEEEECCCC
NPAVRPGAVVTPHNTEIVARSDR
CCCCCCCEEECCCCCEEEEECCC
>Mature Secondary Structure
MQMSGLKILSSSVWLLILALQATGIDASHAQMAPPAVDVAKPLQRTIDRWDEYTGRFEAV
CCCCCHHHHHHHHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
QQVEVRPRVSGFLTSIAFKDGELVRAGELLFTIDPRPYEISVESAQAEIAKAKAQVAQAA
HHHHCCHHHHHHHHHHEECCCCEEEECCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHH
TDVSRAQQLIGSQAITQRDVDARKATLDVAEGQLLAAQASLRAAQLNLEWTRVTAPISGR
HHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEHHHCEEEEEEEEEEEEECCCCCC
ISDRKVDVGNLVDSASSVLTRIVALDPIHFVFEASESDYLRYSRLAAERKRASSRDAPTA
CCCCEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCE
VDVRLADEAEWTWHGSMNFVDNQLGARTGTIRGRAVFANPNLFLIPGEFGRARLFGGKAD
EEEEEECCCCEEECCCCCHHHHHHCCCCCCEECEEEEECCCEEEEECCCCCEEEECCCCC
VLLIPDAAVLSDQASKIVLVVGAGAKLGAKPVRLGPIALGLRSVFEGLSPDDVVVVGGLA
EEECCCHHHHCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHHCCCCCCCEEEECCCC
NPAVRPGAVVTPHNTEIVARSDR
CCCCCCCEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]