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The map label for this gene is 146341319

Identifier: 146341319

GI number: 146341319

Start: 4601733

End: 4603187

Strand: Reverse

Name: 146341319

Synonym: BRADO4401

Alternate gene names: NA

Gene position: 4603187-4601733 (Counterclockwise)

Preceding gene: 146341320

Following gene: 146341318

Centisome position: 61.73

GC content: 66.12

Gene sequence:

>1455_bases
ATGAGCGCTGACAACGAAATCTGCCGCATGGACGCCGTCACCGTTGCGGCAAAGGTCAAGGACAAGACTCTCTCGGCCAT
GGAGGTCACCGAGGCGGTGCTGCGCCGCATGGACAAGCTCGAGCCGCACATCCACGCCTTCTGCACGCCGACACCCGATG
TCGCGCGGGCGGCGGCGGCCGCCGTCGACGCCAAGATCGCCGCGGGCGAGGATCCCGGCCTGCTTGCGGGCGTGCCGATC
GGCATCAAGGATCTGGTTGCGACCAAGGACATCCTCACCGTCATGGGGTCGCTGCTCTATCGCGACTTCGTGCCGGACGA
GGATGACATCGTGGTCGAGCGCCTGAAGGCGGCCGGCGCCGTCATCATCGGCAAGACCAACGTGCCGGAGTTCGGATACA
GCGGCGTCGGCCACAATCCGGTGTTTCCGGCGACGCGCAATCCTTGGAATCTCGACATGACGTCGGGTGGATCGAGCGCC
GGTTCCGGTGCGTCGGTCGCGAGCGGTGTCGCGCCGTTTGCGATCGGCTCCGACGGCGGCGGCTCGATCCGTATTCCCTC
GGCGCTGTGCGGGCTCTACGGCATCAAGGCCTCCATGGGCCGCGTGCCGCTTTATCCAGGGTGCCGCGACGAGCGCTATC
CCGGCGTGTCGAGCTGGGAGTCACTCGAGCATGTCGGTCCGATGAGCCGCACCGTGGCGGACTCGGCGCTGATGCTGAAG
GTGATCACCGGGCCCGATCCGCGCGATCGCTACTCGATCCCGGCCGCCGACTTCGACTATGTCGATGCAACGAAGGAGAG
CATCAAGGGATTGCGCATCGCCTACAGCGAGGACTGGGGCTATGCGCCGGTCGATCCCGAAGTCCGTCGCGTCGTCTCCG
AAGCGGTGCGCGTGTTCGAGACCGATCTCGGCTGCACCGTCGAGCGCGCCAATCCCGGCTGGGACGATCCGTTCCCGCAT
TTCTGGACCATCGTCGCCGGCGACACCGACCTCACCGGGATGCGCCGCATGATGAAGGGGCGCGAGAAGGAGATGTCGCC
GCATCTCGTTGCGCTGCTGCAGCGGCAGTGGACGGCGGAGGAGTTCACGGACGCCAAGATGATCCGGCAGATGGTCTGCA
ACCGCATGTGGCGGTTCATGGCCAACTACGATCTCCTGATCACGCCCACGCTCGCGGTGCCGGCCTTCCCGCTCTACATG
CAGGGGCCGGAGATCATCGAAGACAAGATGGTCACGACCGGGGACTGGCTCTGCTTCACCTTCATCTGCAACCTGACCGG
CCAGCCCGCCGCGACCATTCCCGCGGGCTTCACCAAGACGGGTCTTCCTGTCGGCATGCAGATCATCGGACGCCATCTGG
CTGATAGGACCGTGCTCGCCGCCTCCGGCGCCTTCGAGCAGGCGCGCCCGTGGGCCCACCAATGGCCGAAGCTGTTGAGC
GAGCTCGGCCTCTGA

Upstream 100 bases:

>100_bases
GCGGCCGATATCTGCGCAACGGCGATGCCGCCGCTCCGCGCGACGGCGCCGGATCATTTCGCCGCCTGCCACTTCGCTGT
TTCACCCGCTGGAGGAAACG

Downstream 100 bases:

>100_bases
CACTGCCTCCACCCTCCACCTGAACCCTCCAGAAAGGACGCACGATATGAAGCTTTATCGCAGTCTCGTTGCAGCGGCGT
TGCTCACAGCGTCCGCTGTG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSADNEICRMDAVTVAAKVKDKTLSAMEVTEAVLRRMDKLEPHIHAFCTPTPDVARAAAAAVDAKIAAGEDPGLLAGVPI
GIKDLVATKDILTVMGSLLYRDFVPDEDDIVVERLKAAGAVIIGKTNVPEFGYSGVGHNPVFPATRNPWNLDMTSGGSSA
GSGASVASGVAPFAIGSDGGGSIRIPSALCGLYGIKASMGRVPLYPGCRDERYPGVSSWESLEHVGPMSRTVADSALMLK
VITGPDPRDRYSIPAADFDYVDATKESIKGLRIAYSEDWGYAPVDPEVRRVVSEAVRVFETDLGCTVERANPGWDDPFPH
FWTIVAGDTDLTGMRRMMKGREKEMSPHLVALLQRQWTAEEFTDAKMIRQMVCNRMWRFMANYDLLITPTLAVPAFPLYM
QGPEIIEDKMVTTGDWLCFTFICNLTGQPAATIPAGFTKTGLPVGMQIIGRHLADRTVLAASGAFEQARPWAHQWPKLLS
ELGL

Sequences:

>Translated_484_residues
MSADNEICRMDAVTVAAKVKDKTLSAMEVTEAVLRRMDKLEPHIHAFCTPTPDVARAAAAAVDAKIAAGEDPGLLAGVPI
GIKDLVATKDILTVMGSLLYRDFVPDEDDIVVERLKAAGAVIIGKTNVPEFGYSGVGHNPVFPATRNPWNLDMTSGGSSA
GSGASVASGVAPFAIGSDGGGSIRIPSALCGLYGIKASMGRVPLYPGCRDERYPGVSSWESLEHVGPMSRTVADSALMLK
VITGPDPRDRYSIPAADFDYVDATKESIKGLRIAYSEDWGYAPVDPEVRRVVSEAVRVFETDLGCTVERANPGWDDPFPH
FWTIVAGDTDLTGMRRMMKGREKEMSPHLVALLQRQWTAEEFTDAKMIRQMVCNRMWRFMANYDLLITPTLAVPAFPLYM
QGPEIIEDKMVTTGDWLCFTFICNLTGQPAATIPAGFTKTGLPVGMQIIGRHLADRTVLAASGAFEQARPWAHQWPKLLS
ELGL
>Mature_483_residues
SADNEICRMDAVTVAAKVKDKTLSAMEVTEAVLRRMDKLEPHIHAFCTPTPDVARAAAAAVDAKIAAGEDPGLLAGVPIG
IKDLVATKDILTVMGSLLYRDFVPDEDDIVVERLKAAGAVIIGKTNVPEFGYSGVGHNPVFPATRNPWNLDMTSGGSSAG
SGASVASGVAPFAIGSDGGGSIRIPSALCGLYGIKASMGRVPLYPGCRDERYPGVSSWESLEHVGPMSRTVADSALMLKV
ITGPDPRDRYSIPAADFDYVDATKESIKGLRIAYSEDWGYAPVDPEVRRVVSEAVRVFETDLGCTVERANPGWDDPFPHF
WTIVAGDTDLTGMRRMMKGREKEMSPHLVALLQRQWTAEEFTDAKMIRQMVCNRMWRFMANYDLLITPTLAVPAFPLYMQ
GPEIIEDKMVTTGDWLCFTFICNLTGQPAATIPAGFTKTGLPVGMQIIGRHLADRTVLAASGAFEQARPWAHQWPKLLSE
LGL

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=26.3888888888889, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.9496981891348, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI166795287, Length=245, Percent_Identity=34.2857142857143, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=23.9837398373984, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=464, Percent_Identity=26.7241379310345, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=290, Percent_Identity=29.3103448275862, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=322, Percent_Identity=29.5031055900621, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556278, Length=398, Percent_Identity=27.3869346733668, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=230, Percent_Identity=32.6086956521739, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=234, Percent_Identity=32.0512820512821, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=237, Percent_Identity=33.7552742616034, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=408, Percent_Identity=27.2058823529412, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=236, Percent_Identity=31.3559322033898, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=434, Percent_Identity=25.1152073732719, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=27.0916334661355, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=27.0916334661355, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=27.0916334661355, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=27.0916334661355, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI21356731, Length=484, Percent_Identity=25.4132231404959, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=27.1629778672032, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=22.5433526011561, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648441, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52450; Mature: 52319

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADNEICRMDAVTVAAKVKDKTLSAMEVTEAVLRRMDKLEPHIHAFCTPTPDVARAAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AVDAKIAAGEDPGLLAGVPIGIKDLVATKDILTVMGSLLYRDFVPDEDDIVVERLKAAGA
HHHHHEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCE
VIIGKTNVPEFGYSGVGHNPVFPATRNPWNLDMTSGGSSAGSGASVASGVAPFAIGSDGG
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCCEEECCCCC
GSIRIPSALCGLYGIKASMGRVPLYPGCRDERYPGVSSWESLEHVGPMSRTVADSALMLK
CCEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCEEEEE
VITGPDPRDRYSIPAADFDYVDATKESIKGLRIAYSEDWGYAPVDPEVRRVVSEAVRVFE
EECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
TDLGCTVERANPGWDDPFPHFWTIVAGDTDLTGMRRMMKGREKEMSPHLVALLQRQWTAE
HCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHCCCHHHHHHHHHHCCHH
EFTDAKMIRQMVCNRMWRFMANYDLLITPTLAVPAFPLYMQGPEIIEDKMVTTGDWLCFT
HHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCEEECCCHHHHCCCCCCCCHHHHH
FICNLTGQPAATIPAGFTKTGLPVGMQIIGRHLADRTVLAASGAFEQARPWAHQWPKLLS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHCCCHHHHHHHHHH
ELGL
HHCC
>Mature Secondary Structure 
SADNEICRMDAVTVAAKVKDKTLSAMEVTEAVLRRMDKLEPHIHAFCTPTPDVARAAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AVDAKIAAGEDPGLLAGVPIGIKDLVATKDILTVMGSLLYRDFVPDEDDIVVERLKAAGA
HHHHHEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCE
VIIGKTNVPEFGYSGVGHNPVFPATRNPWNLDMTSGGSSAGSGASVASGVAPFAIGSDGG
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCCEEECCCCC
GSIRIPSALCGLYGIKASMGRVPLYPGCRDERYPGVSSWESLEHVGPMSRTVADSALMLK
CCEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCEEEEE
VITGPDPRDRYSIPAADFDYVDATKESIKGLRIAYSEDWGYAPVDPEVRRVVSEAVRVFE
EECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
TDLGCTVERANPGWDDPFPHFWTIVAGDTDLTGMRRMMKGREKEMSPHLVALLQRQWTAE
HCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHCCCHHHHHHHHHHCCHH
EFTDAKMIRQMVCNRMWRFMANYDLLITPTLAVPAFPLYMQGPEIIEDKMVTTGDWLCFT
HHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCEEECCCHHHHCCCCCCCCHHHHH
FICNLTGQPAATIPAGFTKTGLPVGMQIIGRHLADRTVLAASGAFEQARPWAHQWPKLLS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHCCCHHHHHHHHHH
ELGL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]