BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is braG [H]

Identifier: 146340680

GI number: 146340680

Start: 3893975

End: 3894721

Strand: Direct

Name: braG [H]

Synonym: BRADO3731

Alternate gene names: 146340680

Gene position: 3893975-3894721 (Clockwise)

Preceding gene: 146340679

Following gene: 146340681

Centisome position: 52.22

GC content: 63.19

Gene sequence:

>747_bases
ATGCCTGAGGTGATGACCGCTGCAAAGCTTGGGCAGCCAGCTGCCACCAACACGATTCTCGACGTCAAGAACCTCGAGAG
CTGGTATGGCGAGTCCCACATTCTCCACGGCATGAACTTCAACGTGAATGCCGGCGAGGTCGTCACCCTGCTCGGGCGCA
ACGGCGCCGGCAAGACCACGACTTTGAAGTCCATTATGGGCATCATCGGCAAGCGCACCGGGTCGGTCGCGTTCAATGGC
CAGCAGCTGATCCGCATGACTTCGGACAGAATCGCCCGGCTCGGCATCGCGCTGTGCCCGGAGGAGCGCGGCATCTTCGC
GAGCCTGGACGTGCGCGAGAACCTGCTGCTGCCGCCGGTGGTGCGCCCGGGCGGGCTGTCGCTCGACCAGATCTTCGAGC
TGTTCCCGAACCTGAAGGAGCGGCTGAAGAGCCAGGGCACCAAGCTGTCCGGCGGCGAGCAGCAGATGCTGGCGATCGCG
CGCATCCTGCGTACCGGCGCGAGCTTCCTGATGCTGGACGAGCCGACCGAAGGTCTGGCGCCGGTCATCATCCAGCAGAT
CGGCCACACCATCGCGCGGCTGAAGAAGGAGGGCTTCACGATCCTCCTCGTCGAGCAGAACTTCCGCTTCGCCTCGACCG
TCGCCGACCGCTATTACGTCGTCGAGCACGGCAAGGTCATCGATGGTTTCGCCAATGCCGATCTGTCCGCCAACATGGAC
AAGCTGCATACTTATCTTGGCGTCTGA

Upstream 100 bases:

>100_bases
ACCATCACCGTGCTGACGCGCGGGCAGGTGTTGACCGAGGGACCTTATTCCGAGCTCTCCAAGGACGAGCGCGTCAAGGA
AGCCTATCTGGGAGCCGGAC

Downstream 100 bases:

>100_bases
CCGGCGCCGCGACTGTTTCTTGGAATTGGGAAGTTGGAAATGAAGACTGAATTGATTGCGTCATTGCTGCTGGGCACCGC
GCTCACGCTTGCGGGCGGCA

Product: putative branched-chain amino acid transport system ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPEVMTAAKLGQPAATNTILDVKNLESWYGESHILHGMNFNVNAGEVVTLLGRNGAGKTTTLKSIMGIIGKRTGSVAFNG
QQLIRMTSDRIARLGIALCPEERGIFASLDVRENLLLPPVVRPGGLSLDQIFELFPNLKERLKSQGTKLSGGEQQMLAIA
RILRTGASFLMLDEPTEGLAPVIIQQIGHTIARLKKEGFTILLVEQNFRFASTVADRYYVVEHGKVIDGFANADLSANMD
KLHTYLGV

Sequences:

>Translated_248_residues
MPEVMTAAKLGQPAATNTILDVKNLESWYGESHILHGMNFNVNAGEVVTLLGRNGAGKTTTLKSIMGIIGKRTGSVAFNG
QQLIRMTSDRIARLGIALCPEERGIFASLDVRENLLLPPVVRPGGLSLDQIFELFPNLKERLKSQGTKLSGGEQQMLAIA
RILRTGASFLMLDEPTEGLAPVIIQQIGHTIARLKKEGFTILLVEQNFRFASTVADRYYVVEHGKVIDGFANADLSANMD
KLHTYLGV
>Mature_247_residues
PEVMTAAKLGQPAATNTILDVKNLESWYGESHILHGMNFNVNAGEVVTLLGRNGAGKTTTLKSIMGIIGKRTGSVAFNGQ
QLIRMTSDRIARLGIALCPEERGIFASLDVRENLLLPPVVRPGGLSLDQIFELFPNLKERLKSQGTKLSGGEQQMLAIAR
ILRTGASFLMLDEPTEGLAPVIIQQIGHTIARLKKEGFTILLVEQNFRFASTVADRYYVVEHGKVIDGFANADLSANMDK
LHTYLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI21536376, Length=236, Percent_Identity=29.2372881355932, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI47078218, Length=198, Percent_Identity=31.3131313131313, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45446740, Length=198, Percent_Identity=31.3131313131313, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI27881501, Length=233, Percent_Identity=27.4678111587983, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI30795238, Length=233, Percent_Identity=27.4678111587983, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI116734710, Length=197, Percent_Identity=29.9492385786802, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31657092, Length=203, Percent_Identity=25.1231527093596, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI153792144, Length=208, Percent_Identity=29.3269230769231, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI27477115, Length=221, Percent_Identity=31.6742081447964, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI105990541, Length=194, Percent_Identity=26.8041237113402, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI27436953, Length=196, Percent_Identity=29.5918367346939, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI42741659, Length=261, Percent_Identity=27.2030651340996, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI150417984, Length=224, Percent_Identity=29.0178571428571, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI90421313, Length=219, Percent_Identity=29.2237442922374, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27262624, Length=194, Percent_Identity=25.2577319587629, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI27262626, Length=194, Percent_Identity=25.2577319587629, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI6005701, Length=196, Percent_Identity=29.0816326530612, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI9961252, Length=212, Percent_Identity=27.8301886792453, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4505771, Length=212, Percent_Identity=27.8301886792453, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI9961250, Length=212, Percent_Identity=27.8301886792453, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI42490749, Length=253, Percent_Identity=24.901185770751, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI148612844, Length=220, Percent_Identity=28.1818181818182, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI255708477, Length=220, Percent_Identity=28.1818181818182, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=240, Percent_Identity=39.5833333333333, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1789593, Length=214, Percent_Identity=34.1121495327103, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789672, Length=237, Percent_Identity=30.8016877637131, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=28.968253968254, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787792, Length=222, Percent_Identity=30.1801801801802, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787029, Length=217, Percent_Identity=28.110599078341, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788225, Length=237, Percent_Identity=29.957805907173, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787089, Length=240, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789991, Length=252, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787112, Length=244, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=219, Percent_Identity=27.3972602739726, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788897, Length=248, Percent_Identity=28.2258064516129, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788210, Length=209, Percent_Identity=32.0574162679426, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786872, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790525, Length=221, Percent_Identity=28.5067873303167, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI48995001, Length=241, Percent_Identity=29.4605809128631, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=27.8538812785388, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790190, Length=217, Percent_Identity=27.1889400921659, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788472, Length=222, Percent_Identity=30.6306306306306, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788644, Length=239, Percent_Identity=28.4518828451883, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789873, Length=215, Percent_Identity=26.5116279069767, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790467, Length=220, Percent_Identity=28.6363636363636, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787105, Length=223, Percent_Identity=26.9058295964126, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786253, Length=213, Percent_Identity=29.1079812206573, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081782, Length=226, Percent_Identity=29.2035398230088, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081834, Length=234, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786398, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786698, Length=182, Percent_Identity=28.021978021978, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788165, Length=218, Percent_Identity=25.6880733944954, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787370, Length=227, Percent_Identity=23.7885462555066, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI48994883, Length=246, Percent_Identity=24.7967479674797, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786319, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786703, Length=225, Percent_Identity=29.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994943, Length=224, Percent_Identity=25.8928571428571, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994997, Length=222, Percent_Identity=26.5765765765766, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87081791, Length=225, Percent_Identity=24, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082267, Length=208, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87081709, Length=233, Percent_Identity=24.8927038626609, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI145693107, Length=249, Percent_Identity=22.8915662650602, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786563, Length=213, Percent_Identity=25.8215962441315, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790739, Length=221, Percent_Identity=26.6968325791855, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786654, Length=232, Percent_Identity=25, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=199, Percent_Identity=30.6532663316583, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=203, Percent_Identity=31.5270935960591, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=198, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71996550, Length=262, Percent_Identity=26.7175572519084, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=209, Percent_Identity=28.7081339712919, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988343, Length=219, Percent_Identity=29.2237442922374, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=224, Percent_Identity=25.4464285714286, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=210, Percent_Identity=25.7142857142857, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648314, Length=212, Percent_Identity=26.4150943396226, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24666092, Length=226, Percent_Identity=28.7610619469027, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24643674, Length=221, Percent_Identity=25.3393665158371, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221512771, Length=208, Percent_Identity=27.8846153846154, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28573571, Length=216, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28573573, Length=211, Percent_Identity=26.0663507109005, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585091, Length=228, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007184, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221500365, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397593, Length=212, Percent_Identity=29.2452830188679, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27031; Mature: 26900

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVMTAAKLGQPAATNTILDVKNLESWYGESHILHGMNFNVNAGEVVTLLGRNGAGKTT
CCCHHHHHHCCCCCCCCEEEEHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCHH
TLKSIMGIIGKRTGSVAFNGQQLIRMTSDRIARLGIALCPEERGIFASLDVRENLLLPPV
HHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCCC
VRPGGLSLDQIFELFPNLKERLKSQGTKLSGGEQQMLAIARILRTGASFLMLDEPTEGLA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHH
PVIIQQIGHTIARLKKEGFTILLVEQNFRFASTVADRYYVVEHGKVIDGFANADLSANMD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHEEEEEECCCEECCCCCCCCCCCHH
KLHTYLGV
HHHHHHCC
>Mature Secondary Structure 
PEVMTAAKLGQPAATNTILDVKNLESWYGESHILHGMNFNVNAGEVVTLLGRNGAGKTT
CCHHHHHHCCCCCCCCEEEEHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCHH
TLKSIMGIIGKRTGSVAFNGQQLIRMTSDRIARLGIALCPEERGIFASLDVRENLLLPPV
HHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCCC
VRPGGLSLDQIFELFPNLKERLKSQGTKLSGGEQQMLAIARILRTGASFLMLDEPTEGLA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHH
PVIIQQIGHTIARLKKEGFTILLVEQNFRFASTVADRYYVVEHGKVIDGFANADLSANMD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHEEEEEECCCEECCCCCCCCCCCHH
KLHTYLGV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]