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The map label for this gene is yiaO [H]

Identifier: 146340636

GI number: 146340636

Start: 3851921

End: 3852943

Strand: Reverse

Name: yiaO [H]

Synonym: BRADO3684

Alternate gene names: 146340636

Gene position: 3852943-3851921 (Counterclockwise)

Preceding gene: 146340638

Following gene: 146340635

Centisome position: 51.67

GC content: 63.93

Gene sequence:

>1023_bases
ATGCTTTCACGCCGTCACGTCATCGCCTCCGCGCTTGCTGCGCCTGCCATTCTGCGGTTCGGAACCGGCACGGCCCATGC
GGCAACGACGCTGAAAATCTCCCACCAGTTCCCCGGTGGGACCATCGACAAGGGCGACTTCCGCGACCGGCTGTGCCGGG
TGTTCGCCGAGGAAGTGTCCAAGCGCTCAGGCGGCGAGATTGCCGCCGAGATCTATCCCAACTCGTCACTGATCAAGACG
GTGGCACAGTTTTCGGCGATGCGGAAAGGCGCGCTCGACATCAGTCTGTATCCGATGCCCTACGCTGGCGGCGAGCTTCC
GGAGACCAACATCGGACTGATGCCGGGACTCGTGACCACCTACGACCAGGGCATGCGCTGGAAGAACGAGCCGGTCGGCA
AGGCGCTGAGCGACTTCCTCGCAGACAAGGGCATCATCCTGCTCACCTGGGTCTGGCAGGCGGGCGGCGTCGCCAGCCGC
TCGCGTCCGTTGCTGACCCCAGACGATGCCAAGGGCATGAAGGTGCGCGGCGGCTCGCGCGAGATGGACATGGTGCTGCA
GACCGCGGGCGCGTCCGTGCTCTCCGTGCCATCCAATGAAATCTATACCGCGATGCAGACCGGCGCCTGCGACGCCGGCC
TCACCTCGTCGACCAGCCTGATCTCGTTCCGTCTCGAGGAGGTGGCCAAGGCGCTGACCTCCGGCGCCAAGGCGTCGTAC
TGGTTCATGCTCGAGCCGCTGATGATGTCGAAGGCCTCGTTCGACAAGTTGCCGAAGAACCAGCAGGACATCCTGATGAC
GGTCGGCGCGGAGATGGAGGCGTTCGGACGCAAGGGCGCACAGGAGGACGACATCGAGGTCGCCAAGGTCTACGAAAAGG
CCGGCGCCAAGGTGGTGGAGCTCGATGTCGAGATCGTCAACAAATGGCGAGACATTGCCCGAGACACCGCCTGGAAGGAC
TATGGCGGAAAGTCTGCGAATTGCGCGAAGCTCCTCAAGCTCGCCTCCGATGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GTCGAGTTCGGCTAGAGGTAACCCGGCAGATTGGCTCGTATCGATAATGCCAGCGAACCAGGGTCGGGAACCCAACCCAG
AGTTGTCCAGGGGAAATCTC

Downstream 100 bases:

>100_bases
TTGATGCACGTACCCGTCTCCGACAGTGAGGCCACGCCGACCACCGGCGCCAGCGGACCGCTGGCGCCGGTTCAGCGTGT
GATCTCCGCGCTCAACAGCA

Product: putative TRAP-type C4-dicarboxylate transport system binding periplasmic protein DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLSRRHVIASALAAPAILRFGTGTAHAATTLKISHQFPGGTIDKGDFRDRLCRVFAEEVSKRSGGEIAAEIYPNSSLIKT
VAQFSAMRKGALDISLYPMPYAGGELPETNIGLMPGLVTTYDQGMRWKNEPVGKALSDFLADKGIILLTWVWQAGGVASR
SRPLLTPDDAKGMKVRGGSREMDMVLQTAGASVLSVPSNEIYTAMQTGACDAGLTSSTSLISFRLEEVAKALTSGAKASY
WFMLEPLMMSKASFDKLPKNQQDILMTVGAEMEAFGRKGAQEDDIEVAKVYEKAGAKVVELDVEIVNKWRDIARDTAWKD
YGGKSANCAKLLKLASDVAA

Sequences:

>Translated_340_residues
MLSRRHVIASALAAPAILRFGTGTAHAATTLKISHQFPGGTIDKGDFRDRLCRVFAEEVSKRSGGEIAAEIYPNSSLIKT
VAQFSAMRKGALDISLYPMPYAGGELPETNIGLMPGLVTTYDQGMRWKNEPVGKALSDFLADKGIILLTWVWQAGGVASR
SRPLLTPDDAKGMKVRGGSREMDMVLQTAGASVLSVPSNEIYTAMQTGACDAGLTSSTSLISFRLEEVAKALTSGAKASY
WFMLEPLMMSKASFDKLPKNQQDILMTVGAEMEAFGRKGAQEDDIEVAKVYEKAGAKVVELDVEIVNKWRDIARDTAWKD
YGGKSANCAKLLKLASDVAA
>Mature_340_residues
MLSRRHVIASALAAPAILRFGTGTAHAATTLKISHQFPGGTIDKGDFRDRLCRVFAEEVSKRSGGEIAAEIYPNSSLIKT
VAQFSAMRKGALDISLYPMPYAGGELPETNIGLMPGLVTTYDQGMRWKNEPVGKALSDFLADKGIILLTWVWQAGGVASR
SRPLLTPDDAKGMKVRGGSREMDMVLQTAGASVLSVPSNEIYTAMQTGACDAGLTSSTSLISFRLEEVAKALTSGAKASY
WFMLEPLMMSKASFDKLPKNQQDILMTVGAEMEAFGRKGAQEDDIEVAKVYEKAGAKVVELDVEIVNKWRDIARDTAWKD
YGGKSANCAKLLKLASDVAA

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=251, Percent_Identity=27.0916334661355, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36638; Mature: 36638

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRHVIASALAAPAILRFGTGTAHAATTLKISHQFPGGTIDKGDFRDRLCRVFAEEVS
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KRSGGEIAAEIYPNSSLIKTVAQFSAMRKGALDISLYPMPYAGGELPETNIGLMPGLVTT
HCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
YDQGMRWKNEPVGKALSDFLADKGIILLTWVWQAGGVASRSRPLLTPDDAKGMKVRGGSR
HHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCH
EMDMVLQTAGASVLSVPSNEIYTAMQTGACDAGLTSSTSLISFRLEEVAKALTSGAKASY
HHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WFMLEPLMMSKASFDKLPKNQQDILMTVGAEMEAFGRKGAQEDDIEVAKVYEKAGAKVVE
EEEECHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCEEEE
LDVEIVNKWRDIARDTAWKDYGGKSANCAKLLKLASDVAA
EHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSRRHVIASALAAPAILRFGTGTAHAATTLKISHQFPGGTIDKGDFRDRLCRVFAEEVS
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KRSGGEIAAEIYPNSSLIKTVAQFSAMRKGALDISLYPMPYAGGELPETNIGLMPGLVTT
HCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
YDQGMRWKNEPVGKALSDFLADKGIILLTWVWQAGGVASRSRPLLTPDDAKGMKVRGGSR
HHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCH
EMDMVLQTAGASVLSVPSNEIYTAMQTGACDAGLTSSTSLISFRLEEVAKALTSGAKASY
HHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WFMLEPLMMSKASFDKLPKNQQDILMTVGAEMEAFGRKGAQEDDIEVAKVYEKAGAKVVE
EEEECHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCEEEE
LDVEIVNKWRDIARDTAWKDYGGKSANCAKLLKLASDVAA
EHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]