Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 146340620

GI number: 146340620

Start: 3836283

End: 3836735

Strand: Reverse

Name: gloA [H]

Synonym: BRADO3665

Alternate gene names: 146340620

Gene position: 3836735-3836283 (Counterclockwise)

Preceding gene: 146340623

Following gene: 146340619

Centisome position: 51.45

GC content: 62.91

Gene sequence:

>453_bases
ATGCGTTATCTCCACACCATGCTGCGCGTGCGCAATCTCGACGTCGCGCTCAAATTCTATTCCGATGCGCTCGGCCTCAA
GGAGGTGCGCCGGATCGACAACGACAAGGGCCGCTTCACGCTGGTGTTTCTTTGCGCACCCGAGGATGAACACCTGCTGA
AGAGCCTGCCGCCGACGCGCGGAGCACCGCTGGTCGAGCTGACCTACAATTGGGACGAGGAAAAATACGGCGAGGACCGC
TTCTTCGGCCATCTCGCCTATGAGGTCGACGACATCTACGCCACCTGCGATCGCCTGATGAAGCTCGGCGTCACCATCAA
CCGGCCGCCGCGCGACGGCAACATGGCGTTCGTGCGCTCGCCCGACCTGCACTCGATAGAGCTGTTGCAGAAGGGCGAGC
CGAAGGCGCCGGCCGAGCCGTGGCTGTCGATGCCGAACACCGGACACTGGTAG

Upstream 100 bases:

>100_bases
GCATCGCGGCAAGCTGCGCAAGCCTGCCGAATGCTGCTATGGATCGCCGTCCGCAGGCGGCCGTTGAACATCCGCTTCAA
GCTCAGGGAGACAAGGAACG

Downstream 100 bases:

>100_bases
GCGGCATGTATCGTTTCCTGGTCCGTCTGCGTGATCCGAGCTATCGGCTCGTGCTGCCGGATGACCGGGAAGTCCCGGCT
GGCGTGCGCGAGCAGGACTG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MRYLHTMLRVRNLDVALKFYSDALGLKEVRRIDNDKGRFTLVFLCAPEDEHLLKSLPPTRGAPLVELTYNWDEEKYGEDR
FFGHLAYEVDDIYATCDRLMKLGVTINRPPRDGNMAFVRSPDLHSIELLQKGEPKAPAEPWLSMPNTGHW

Sequences:

>Translated_150_residues
MRYLHTMLRVRNLDVALKFYSDALGLKEVRRIDNDKGRFTLVFLCAPEDEHLLKSLPPTRGAPLVELTYNWDEEKYGEDR
FFGHLAYEVDDIYATCDRLMKLGVTINRPPRDGNMAFVRSPDLHSIELLQKGEPKAPAEPWLSMPNTGHW
>Mature_150_residues
MRYLHTMLRVRNLDVALKFYSDALGLKEVRRIDNDKGRFTLVFLCAPEDEHLLKSLPPTRGAPLVELTYNWDEEKYGEDR
FFGHLAYEVDDIYATCDRLMKLGVTINRPPRDGNMAFVRSPDLHSIELLQKGEPKAPAEPWLSMPNTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=34.2465753424658, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1787940, Length=137, Percent_Identity=35.036496350365, Blast_Score=85, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323639, Length=146, Percent_Identity=32.8767123287671, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=32.4137931034483, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 17374; Mature: 17374

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMLRVRNLDVALKFYSDALGLKEVRRIDNDKGRFTLVFLCAPEDEHLLKSLPPTR
CHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCCC
GAPLVELTYNWDEEKYGEDRFFGHLAYEVDDIYATCDRLMKLGVTINRPPRDGNMAFVRS
CCCEEEEEECCCHHHCCCCHHEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEC
PDLHSIELLQKGEPKAPAEPWLSMPNTGHW
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYLHTMLRVRNLDVALKFYSDALGLKEVRRIDNDKGRFTLVFLCAPEDEHLLKSLPPTR
CHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCCC
GAPLVELTYNWDEEKYGEDRFFGHLAYEVDDIYATCDRLMKLGVTINRPPRDGNMAFVRS
CCCEEEEEECCCHHHCCCCHHEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEC
PDLHSIELLQKGEPKAPAEPWLSMPNTGHW
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]