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The map label for this gene is pleD [H]

Identifier: 146340506

GI number: 146340506

Start: 3739565

End: 3740632

Strand: Reverse

Name: pleD [H]

Synonym: BRADO3548

Alternate gene names: 146340506

Gene position: 3740632-3739565 (Counterclockwise)

Preceding gene: 146340507

Following gene: 146340494

Centisome position: 50.17

GC content: 64.61

Gene sequence:

>1068_bases
GTGGTCAATGCGCTTGACGAACACGAACGCACGATGGCCTTCGCGGAAGTCGCGCTCAGCCAGATTAAAACACTCCGTCA
CTCGGCCAATCCGCGCAACTATGAGATCTGGTACGTCTACGCGACCGGCTACAATGCGCAGCTGAACGAGATCATCAACG
GCACGCTGGCGCGCAACGGCCGGCTGTCGCAGTCCGAGCTCGACCAGCTCTACGAATCCTATCTCGCGCATCTGAAGGCG
TCAGACCGCATCGACAAGGTCGGGGCGCGCGTTGTCGGCGAGATCGACGACCTCATGGGGATGCTCACGGACGCGCTCGG
CCGCACCGCGAGCTACGATGCGAGCCTGGCCGGCGCCGATCGCATGCTCGCCTCTGCGACCAGCCGCGAGCAGCTCGTCG
ACATCATCGAGGCACTGGCGCATTCGACCAAGCAGATGCGGGAGACGAACCGGATCCTCGAGGAGCGCCTGTCGCTGTCC
AAGGATGAAATCAGCGGACTGCAGCACAGCCTGGAAGCCATTCGCGCCGAAAGCCTCACCGACCCGCTGACCGGACTTGG
CAACCGCAAGTATTTCGACCGCATGATCGAGACGGCGGTGCTGACGGGTCTTGCGACCAACCAGCCTTTGTCGCTGCTGA
TGTTCGACATCGACCATTTCAAGTCGTTCAACGACTCCTACGGTCACCTCACCGGCGACCAGGTGCTCCGCCTGGTCGGC
CTGACGCTGAAGCACGCGGTCAAGGGGCAGGACATCGCCGCGCGTTACGGCGGCGAAGAATTCGCGGTGGTGCTGCCGAA
CACGGCGCTGCGGCAGGCGCTGGCGGTCGCCGACCAGATTCGCCGCGCGGTCACCGCGAAAGAGTTGAAGAAGAAGTCGA
CCGGCGAGATCCTGGGCCGCGTGACCATCTCGGTCGGCGTCTCCATGCTCCGCCCCGGCGACGACACCGACGCGCTGATC
GAGCGCGCCGACGCCTGCCTCTATGCCGCCAAGCACAACGGCCGCAATCGCGTGGTGTGCGAGAGCGATCCCGAATACGC
GACAGCGACGCGCGGCCAGGTCGCCTGA

Upstream 100 bases:

>100_bases
ACGGCCGTATTTACCCCCGGTTCACAAACGTCATTTAGCTTGCCGGCATTGTTTATTTCATTGCTGTGACGGCAACGCGG
GCGATTGGGGAGAACCTGTT

Downstream 100 bases:

>100_bases
CGCAAGCAAGCCGTTCGTCCGGCGATTAGGCTTTTCACCGCGCCTGCTTCGCCGCCTTGCGCGGCGCTTTCCGCGCCGAG
GCTCCGGTTGCGCGGCTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MVNALDEHERTMAFAEVALSQIKTLRHSANPRNYEIWYVYATGYNAQLNEIINGTLARNGRLSQSELDQLYESYLAHLKA
SDRIDKVGARVVGEIDDLMGMLTDALGRTASYDASLAGADRMLASATSREQLVDIIEALAHSTKQMRETNRILEERLSLS
KDEISGLQHSLEAIRAESLTDPLTGLGNRKYFDRMIETAVLTGLATNQPLSLLMFDIDHFKSFNDSYGHLTGDQVLRLVG
LTLKHAVKGQDIAARYGGEEFAVVLPNTALRQALAVADQIRRAVTAKELKKKSTGEILGRVTISVGVSMLRPGDDTDALI
ERADACLYAAKHNGRNRVVCESDPEYATATRGQVA

Sequences:

>Translated_355_residues
MVNALDEHERTMAFAEVALSQIKTLRHSANPRNYEIWYVYATGYNAQLNEIINGTLARNGRLSQSELDQLYESYLAHLKA
SDRIDKVGARVVGEIDDLMGMLTDALGRTASYDASLAGADRMLASATSREQLVDIIEALAHSTKQMRETNRILEERLSLS
KDEISGLQHSLEAIRAESLTDPLTGLGNRKYFDRMIETAVLTGLATNQPLSLLMFDIDHFKSFNDSYGHLTGDQVLRLVG
LTLKHAVKGQDIAARYGGEEFAVVLPNTALRQALAVADQIRRAVTAKELKKKSTGEILGRVTISVGVSMLRPGDDTDALI
ERADACLYAAKHNGRNRVVCESDPEYATATRGQVA
>Mature_355_residues
MVNALDEHERTMAFAEVALSQIKTLRHSANPRNYEIWYVYATGYNAQLNEIINGTLARNGRLSQSELDQLYESYLAHLKA
SDRIDKVGARVVGEIDDLMGMLTDALGRTASYDASLAGADRMLASATSREQLVDIIEALAHSTKQMRETNRILEERLSLS
KDEISGLQHSLEAIRAESLTDPLTGLGNRKYFDRMIETAVLTGLATNQPLSLLMFDIDHFKSFNDSYGHLTGDQVLRLVG
LTLKHAVKGQDIAARYGGEEFAVVLPNTALRQALAVADQIRRAVTAKELKKKSTGEILGRVTISVGVSMLRPGDDTDALI
ERADACLYAAKHNGRNRVVCESDPEYATATRGQVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=42.1383647798742, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=38.3233532934132, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=37.1428571428571, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=181, Percent_Identity=39.2265193370166, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787802, Length=217, Percent_Identity=33.6405529953917, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=35.6020942408377, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.2201257861635, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=32.3529411764706, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788956, Length=183, Percent_Identity=33.879781420765, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=24.0963855421687, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=29.4478527607362, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39053; Mature: 39053

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNALDEHERTMAFAEVALSQIKTLRHSANPRNYEIWYVYATGYNAQLNEIINGTLARNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHCCHHHCCC
RLSQSELDQLYESYLAHLKASDRIDKVGARVVGEIDDLMGMLTDALGRTASYDASLAGAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RMLASATSREQLVDIIEALAHSTKQMRETNRILEERLSLSKDEISGLQHSLEAIRAESLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DPLTGLGNRKYFDRMIETAVLTGLATNQPLSLLMFDIDHFKSFNDSYGHLTGDQVLRLVG
CHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LTLKHAVKGQDIAARYGGEEFAVVLPNTALRQALAVADQIRRAVTAKELKKKSTGEILGR
HHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTISVGVSMLRPGDDTDALIERADACLYAAKHNGRNRVVCESDPEYATATRGQVA
HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MVNALDEHERTMAFAEVALSQIKTLRHSANPRNYEIWYVYATGYNAQLNEIINGTLARNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHCCHHHCCC
RLSQSELDQLYESYLAHLKASDRIDKVGARVVGEIDDLMGMLTDALGRTASYDASLAGAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RMLASATSREQLVDIIEALAHSTKQMRETNRILEERLSLSKDEISGLQHSLEAIRAESLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DPLTGLGNRKYFDRMIETAVLTGLATNQPLSLLMFDIDHFKSFNDSYGHLTGDQVLRLVG
CHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LTLKHAVKGQDIAARYGGEEFAVVLPNTALRQALAVADQIRRAVTAKELKKKSTGEILGR
HHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTISVGVSMLRPGDDTDALIERADACLYAAKHNGRNRVVCESDPEYATATRGQVA
HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA