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The map label for this gene is tyrS [H]

Identifier: 146340490

GI number: 146340490

Start: 3724592

End: 3725845

Strand: Reverse

Name: tyrS [H]

Synonym: BRADO3532

Alternate gene names: 146340490

Gene position: 3725845-3724592 (Counterclockwise)

Preceding gene: 146340493

Following gene: 146340489

Centisome position: 49.97

GC content: 65.79

Gene sequence:

>1254_bases
ATGACTGGCTTCAAGTCCGATTTCCTGAACGTGCTGCAGAGCCGCGGGTTCATTCATCAGTGCTCGGATTTCGAGGGCCT
GGATACGCTCGCCGCCAAGGGCCAGGCGACTGCCTATGTCGGCTATGACTGCACCGCACGCTCGCTGCACATCGGCAACT
TCCTGACCATGATGATGCTGTACTGGCTGCAGCAGACCGGGAACAAGCCGATCACCCTGATGGGCGGCGGCACCACCATG
GTCGGCGACCCCTCCGGCAAGGACGAGACGCGGGCGATGCGCACGGTCGAGGAGATCGAGGCCAACAAGGCCTCGATCCG
CGGCGTGTTCGCAAAAGTGCTGCGCTATGGCGACGGCGCCACCGACGCCATCATGCTCGACAACGCCGAGTGGCTGACCA
AGCTGAACTGGATCGAGATGCTGCGCGACATCGGCCGGCACTTCTCGGTTAACCGAATGCTGACCATGGACTCCGTGCGC
CTGCGGCTCGAGCGCGAGCAAGAGATGAGCTTCATCGAGTTCAACTACATGGTCTGCCAGGCCTACGACTTCGTCGAGCT
GTCACGCCGCACCGGCTGCCGGCTGCAGATGGGCGGCTCCGACCAGTGGGGCAACATCGTCAACGGCGTCGAGCTCGGCC
GCCGCATGGGCACCGAGCAGCTGTTTGCGCTGACCACGCCGCTGCTCACCACCGCCTCCGGCGCCAAGATGGGCAAGACC
GCGCAGGGCGCGATCTGGCTCAATGCCGACCAGTGCAGCCCGTATGAGTTCTGGCAGTACTGGCGCAACACCGAGGACGC
CGACGTCGTCAAGTTCCTCAAGCTCTTCACGACCCTGCCGCTGGCCGAGATCGAGAAGCTCGGCGCGCTCCAGGGAGCCG
AGATCAACGAGGCCAAGAAGGCGCTGGCGGATGCGGCGACCACGCTGCTGCACGGCGGGGAGGCCGCGCGCACCGCGGCC
GAGACTGCCCGCCGGACCTTCGAGGAAGGCGCGATCGCCGAGAACCTGCCGAAAGTCGAGGTCGCCAGGAGCGAGCTCGA
CGCCGGTCTCGGCGTGCTCAATGCCTTCGTCAAGGCCGGGCTCGTCGCCTCCAACGGCGAGGCGCGGCGGCAGATCAAGG
GCGGCGGCCTGCGCGTCAACGACGTCGCGGTCACCGACGAGAAGATGACGCTGACACGGGCGGATCTGACCAGCGAGGGC
GTGATCAAGCTGTCGCTGGGCAAGAAGCGCCACGTGCTGGTCAAGCCTGCATAG

Upstream 100 bases:

>100_bases
CCTATAATGCCACAACGCCTGGACCGGCGGCAGCCGCTCTCCTGCACCGGGCCGCGACATCAGGCCAATACCGTAAACAA
ATGCTCAGAGTCCGCCGACC

Downstream 100 bases:

>100_bases
GGCCATTCGCTTTTGCCTCCTTGTCAGGGTGCGCCGGACGGCGCTCCCTGGTAGCCATGACCAAGAGCGCGCCACCAGCG
CCTGCGCAAGCCGAGGAAAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTGFKSDFLNVLQSRGFIHQCSDFEGLDTLAAKGQATAYVGYDCTARSLHIGNFLTMMMLYWLQQTGNKPITLMGGGTTM
VGDPSGKDETRAMRTVEEIEANKASIRGVFAKVLRYGDGATDAIMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVR
LRLEREQEMSFIEFNYMVCQAYDFVELSRRTGCRLQMGGSDQWGNIVNGVELGRRMGTEQLFALTTPLLTTASGAKMGKT
AQGAIWLNADQCSPYEFWQYWRNTEDADVVKFLKLFTTLPLAEIEKLGALQGAEINEAKKALADAATTLLHGGEAARTAA
ETARRTFEEGAIAENLPKVEVARSELDAGLGVLNAFVKAGLVASNGEARRQIKGGGLRVNDVAVTDEKMTLTRADLTSEG
VIKLSLGKKRHVLVKPA

Sequences:

>Translated_417_residues
MTGFKSDFLNVLQSRGFIHQCSDFEGLDTLAAKGQATAYVGYDCTARSLHIGNFLTMMMLYWLQQTGNKPITLMGGGTTM
VGDPSGKDETRAMRTVEEIEANKASIRGVFAKVLRYGDGATDAIMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVR
LRLEREQEMSFIEFNYMVCQAYDFVELSRRTGCRLQMGGSDQWGNIVNGVELGRRMGTEQLFALTTPLLTTASGAKMGKT
AQGAIWLNADQCSPYEFWQYWRNTEDADVVKFLKLFTTLPLAEIEKLGALQGAEINEAKKALADAATTLLHGGEAARTAA
ETARRTFEEGAIAENLPKVEVARSELDAGLGVLNAFVKAGLVASNGEARRQIKGGGLRVNDVAVTDEKMTLTRADLTSEG
VIKLSLGKKRHVLVKPA
>Mature_416_residues
TGFKSDFLNVLQSRGFIHQCSDFEGLDTLAAKGQATAYVGYDCTARSLHIGNFLTMMMLYWLQQTGNKPITLMGGGTTMV
GDPSGKDETRAMRTVEEIEANKASIRGVFAKVLRYGDGATDAIMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVRL
RLEREQEMSFIEFNYMVCQAYDFVELSRRTGCRLQMGGSDQWGNIVNGVELGRRMGTEQLFALTTPLLTTASGAKMGKTA
QGAIWLNADQCSPYEFWQYWRNTEDADVVKFLKLFTTLPLAEIEKLGALQGAEINEAKKALADAATTLLHGGEAARTAAE
TARRTFEEGAIAENLPKVEVARSELDAGLGVLNAFVKAGLVASNGEARRQIKGGGLRVNDVAVTDEKMTLTRADLTSEGV
IKLSLGKKRHVLVKPA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=35.036496350365, Blast_Score=234, Evalue=8e-62,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=38.3886255924171, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI25145802, Length=295, Percent_Identity=33.8983050847458, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25145805, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=29.9319727891156, Blast_Score=192, Evalue=7e-50,
Organism=Drosophila melanogaster, GI20130383, Length=432, Percent_Identity=34.0277777777778, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45890; Mature: 45759

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFKSDFLNVLQSRGFIHQCSDFEGLDTLAAKGQATAYVGYDCTARSLHIGNFLTMMML
CCCCHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHH
YWLQQTGNKPITLMGGGTTMVGDPSGKDETRAMRTVEEIEANKASIRGVFAKVLRYGDGA
HHHHHCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
TDAIMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVRLRLEREQEMSFIEFNYMVCQ
CCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCEEEEEEHEEE
AYDFVELSRRTGCRLQMGGSDQWGNIVNGVELGRRMGTEQLFALTTPLLTTASGAKMGKT
HHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC
AQGAIWLNADQCSPYEFWQYWRNTEDADVVKFLKLFTTLPLAEIEKLGALQGAEINEAKK
CCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHH
ALADAATTLLHGGEAARTAAETARRTFEEGAIAENLPKVEVARSELDAGLGVLNAFVKAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
LVASNGEARRQIKGGGLRVNDVAVTDEKMTLTRADLTSEGVIKLSLGKKRHVLVKPA
EEECCCHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCEEEEEECC
>Mature Secondary Structure 
TGFKSDFLNVLQSRGFIHQCSDFEGLDTLAAKGQATAYVGYDCTARSLHIGNFLTMMML
CCCHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHH
YWLQQTGNKPITLMGGGTTMVGDPSGKDETRAMRTVEEIEANKASIRGVFAKVLRYGDGA
HHHHHCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
TDAIMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVRLRLEREQEMSFIEFNYMVCQ
CCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCEEEEEEHEEE
AYDFVELSRRTGCRLQMGGSDQWGNIVNGVELGRRMGTEQLFALTTPLLTTASGAKMGKT
HHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC
AQGAIWLNADQCSPYEFWQYWRNTEDADVVKFLKLFTTLPLAEIEKLGALQGAEINEAKK
CCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHH
ALADAATTLLHGGEAARTAAETARRTFEEGAIAENLPKVEVARSELDAGLGVLNAFVKAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
LVASNGEARRQIKGGGLRVNDVAVTDEKMTLTRADLTSEGVIKLSLGKKRHVLVKPA
EEECCCHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]