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The map label for this gene is gcvA [H]

Identifier: 146340386

GI number: 146340386

Start: 3606866

End: 3607753

Strand: Direct

Name: gcvA [H]

Synonym: BRADO3419

Alternate gene names: 146340386

Gene position: 3606866-3607753 (Clockwise)

Preceding gene: 146340384

Following gene: 146340387

Centisome position: 48.37

GC content: 71.06

Gene sequence:

>888_bases
ATGACGCGACCTCGATTGCCCTCTCTCATCGCGCTGCGCGCCTTCGAGGCCGTCGGGCGCCGGGGCAGCGTTCGCGCCGC
AGGCGACGAACTCGCCGTCAGCCATACCGTCGTCTCCCGTCATCTGCAGAACCTGCAGCGCAGCCTCGGCGTCGCCTTGG
TCCGCGCCGAGGGGCGGGGCCTTGCGCTGACCGAGGCCGGCCGGCAGTTCCATGCCGAGGTCACCCAGGCCTTCGACATC
ATCGCCCGGGCGACGACCGCGGTTCGGCCCGCGGCACGGCCAACGTTGAACATCTGGTGCATCCCCGGCCTCGCCAACCG
CCGCCTGCTGGCGCGGCTGCCCGAGCTGACCGGCCCGCCGCGCAACTGGGACATCAACCTGCAACCGACCTTGTCGCATC
CAGACCTGGCGCGCGGCGAGGCCGATGCCGAGATCGTCTATGCCGAGGATCTGGAGCGGGTCAGCGGCACGCTGGCCGAG
GAGCTGGTGCGGCCGCGGGTGTTTCCGGTGGCGAGCCCGGCCTATCTGGCGCGCTTCCCGGAGATCGCCACCTTCGCCGG
CCTCGCCCGCGCGTCGCTGATCCACGAGGAATCGACGGCGCAATGGGAGCGCTGGTTCGCACTGGCCGGCCATCACGACC
CCGTCATCCTGCGTGGCCAAAGGCTGTGGCACGCGCATCTCGCCATCGAGGCGGCGCGCTTCGGGCAGGGCGTCGCGCTC
GCCAACGAGGTGCTGGTCGCGGACGACATGCGCAGCGGCGCGCTGGTCGAGGTGATGCCGTCGTCCGTGCAGATGGGCGC
CTATCGACTCGTGGCGCTCAAGGAGCGCTGGGACGAGCCGGCGATCGTTGCGCTGCGTGCCTGGCTGAAGAAGGTGCTGG
CGGAGTAG

Upstream 100 bases:

>100_bases
GGCCGGCGCTCCGACATCAGCCGCGCAACGGACGAATCGTCGCGGCTCGCCATTCCGGCGGGCGAGGGCAGGGGATCGCG
CAGGCGGACACTGTGCGAGC

Downstream 100 bases:

>100_bases
GCGCCGCATCCCGCCGTCTGGTGCCTTCAAGTCACCCGTCGTGTGGAACAAGCTGATTGATCGCGCCCGCCCTCGGCCTA
GCCTCTTGCTCAGTTGTCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTRPRLPSLIALRAFEAVGRRGSVRAAGDELAVSHTVVSRHLQNLQRSLGVALVRAEGRGLALTEAGRQFHAEVTQAFDI
IARATTAVRPAARPTLNIWCIPGLANRRLLARLPELTGPPRNWDINLQPTLSHPDLARGEADAEIVYAEDLERVSGTLAE
ELVRPRVFPVASPAYLARFPEIATFAGLARASLIHEESTAQWERWFALAGHHDPVILRGQRLWHAHLAIEAARFGQGVAL
ANEVLVADDMRSGALVEVMPSSVQMGAYRLVALKERWDEPAIVALRAWLKKVLAE

Sequences:

>Translated_295_residues
MTRPRLPSLIALRAFEAVGRRGSVRAAGDELAVSHTVVSRHLQNLQRSLGVALVRAEGRGLALTEAGRQFHAEVTQAFDI
IARATTAVRPAARPTLNIWCIPGLANRRLLARLPELTGPPRNWDINLQPTLSHPDLARGEADAEIVYAEDLERVSGTLAE
ELVRPRVFPVASPAYLARFPEIATFAGLARASLIHEESTAQWERWFALAGHHDPVILRGQRLWHAHLAIEAARFGQGVAL
ANEVLVADDMRSGALVEVMPSSVQMGAYRLVALKERWDEPAIVALRAWLKKVLAE
>Mature_294_residues
TRPRLPSLIALRAFEAVGRRGSVRAAGDELAVSHTVVSRHLQNLQRSLGVALVRAEGRGLALTEAGRQFHAEVTQAFDII
ARATTAVRPAARPTLNIWCIPGLANRRLLARLPELTGPPRNWDINLQPTLSHPDLARGEADAEIVYAEDLERVSGTLAEE
LVRPRVFPVASPAYLARFPEIATFAGLARASLIHEESTAQWERWFALAGHHDPVILRGQRLWHAHLAIEAARFGQGVALA
NEVLVADDMRSGALVEVMPSSVQMGAYRLVALKERWDEPAIVALRAWLKKVLAE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=29.2682926829268, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=26.5957446808511, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=25.3246753246753, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32422; Mature: 32291

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPRLPSLIALRAFEAVGRRGSVRAAGDELAVSHTVVSRHLQNLQRSLGVALVRAEGRG
CCCCCCCHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
LALTEAGRQFHAEVTQAFDIIARATTAVRPAARPTLNIWCIPGLANRRLLARLPELTGPP
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCHHCCCCC
RNWDINLQPTLSHPDLARGEADAEIVYAEDLERVSGTLAEELVRPRVFPVASPAYLARFP
CCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
EIATFAGLARASLIHEESTAQWERWFALAGHHDPVILRGQRLWHAHLAIEAARFGQGVAL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
ANEVLVADDMRSGALVEVMPSSVQMGAYRLVALKERWDEPAIVALRAWLKKVLAE
HHHEEEECCCCCCCEEEECCCCHHHHHHHEEEHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRPRLPSLIALRAFEAVGRRGSVRAAGDELAVSHTVVSRHLQNLQRSLGVALVRAEGRG
CCCCCCHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
LALTEAGRQFHAEVTQAFDIIARATTAVRPAARPTLNIWCIPGLANRRLLARLPELTGPP
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCHHCCCCC
RNWDINLQPTLSHPDLARGEADAEIVYAEDLERVSGTLAEELVRPRVFPVASPAYLARFP
CCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
EIATFAGLARASLIHEESTAQWERWFALAGHHDPVILRGQRLWHAHLAIEAARFGQGVAL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
ANEVLVADDMRSGALVEVMPSSVQMGAYRLVALKERWDEPAIVALRAWLKKVLAE
HHHEEEECCCCCCCEEEECCCCHHHHHHHEEEHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]