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The map label for this gene is intZ [H]

Identifier: 146340202

GI number: 146340202

Start: 3419960

End: 3421084

Strand: Reverse

Name: intZ [H]

Synonym: BRADO3220

Alternate gene names: 146340202

Gene position: 3421084-3419960 (Counterclockwise)

Preceding gene: 146340203

Following gene: 146340193

Centisome position: 45.88

GC content: 61.78

Gene sequence:

>1125_bases
ATGCAGTTTCTAACTCAAAAGCCCGAGATCACCGCCAAGAACTATCTCTCCGTCGGGCCCGGCGAATACCCGTGCGGCAA
CACCCTCTACCTGATCGTCACCCCAAGCGGCGGCCGCCGCTGGGCGTTCCGCTACCAGCGCAACGGCATCGTCAAGAAGA
TGGGTTTTGGCTCGGCCAAGGAAACCGGCCTGAAGCTCTCCGAGGCCCGGGACAAGGCGATTGACGCTCTGCGGCTGCTA
GCGAAGGGGACCGATCCGCGAGAGCACCGTGACGTCGAGAAACGCCGCATTCAGGGATCGCGCCAGTTCGGCGAATTCGC
CGAGGAATGGCGGCGAACCTACGAGATCGGCATGGGGCACAAGGCCGCCAGGGCAAAGCTGAAGCGCATCGTGCAGGTCG
TCTGCCGCCCGCTCCACAAGCACTGGGTCCACGAGATCGAAACCGCCCATGTCGTCAACGTGCTCATGCCAATCTGGCAG
CAGCCCGAGACCTCCCGCTCCGCTCGCCAGATCATCAAGAAGATTCTCGACGCGGCGATCGCCCACAACCTGCGACCGAA
AGACAACCCCGCTGACTGGGCCAGCCGACTGCAACCGATCATGCCCAGGCAGCGGCGGCGTGGCACGATACGGGGCGGCC
ACAAGGCGATGGACTACCAGGACCTGCCGGCGTTCATGCACAAGCTCAGCGCCATCTCCACGCAAAGCGCGCGGGCGCTC
GAGGTCATCATCCTGACCCTGGGACGAACCGCCGAAGTTCAGAACATGCGCTGGGCGCAACTCGATCTCGATAACGGAGT
CTGGGATCTTGGCTTTCTCGGCACCAAGAACGAGCGGTTGAAGCGTACGCCGTTGCCCCGGCAGACGCTGACCTATTTGC
GCGAGGCTTACGAGAGCCGGGTCAATGACGAATTCGTGTTTCCCGGGCGCAGTTTAAAGCGGCCGATGAGCAACATGACC
ATGCTGAAATATCTCAAGGAGCTCACCGGAGACGACTCCCTGACAGTGCACGGCTTCCGCACGACGTTTCGAACCTGGGC
GCAGGAGCAAACCCACTTCGAGGAGGAAATCGTCGAGCATTGCCTGCACCACATCACGGGCGATGATGCCGGAAAAGGCC
TATAA

Upstream 100 bases:

>100_bases
CCGCCAGACCGTCCATTTGGCCATCCCTCGATCTGAGTTAGAATCCCCCTCACCTGAAACGCTGCATTTTTCGGCGAGGG
ACAGGAGTTTCGAATCTCCC

Downstream 100 bases:

>100_bases
GCGCGGCGAGGCCCTGAAGAAGCGACTGCATGTGATGCAGACATTCGCGGACTTCGCAACGCAACCACCAAAGAGCAATG
TGACCCCGATGAAGGTCGCC

Product: putative phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MQFLTQKPEITAKNYLSVGPGEYPCGNTLYLIVTPSGGRRWAFRYQRNGIVKKMGFGSAKETGLKLSEARDKAIDALRLL
AKGTDPREHRDVEKRRIQGSRQFGEFAEEWRRTYEIGMGHKAARAKLKRIVQVVCRPLHKHWVHEIETAHVVNVLMPIWQ
QPETSRSARQIIKKILDAAIAHNLRPKDNPADWASRLQPIMPRQRRRGTIRGGHKAMDYQDLPAFMHKLSAISTQSARAL
EVIILTLGRTAEVQNMRWAQLDLDNGVWDLGFLGTKNERLKRTPLPRQTLTYLREAYESRVNDEFVFPGRSLKRPMSNMT
MLKYLKELTGDDSLTVHGFRTTFRTWAQEQTHFEEEIVEHCLHHITGDDAGKGL

Sequences:

>Translated_374_residues
MQFLTQKPEITAKNYLSVGPGEYPCGNTLYLIVTPSGGRRWAFRYQRNGIVKKMGFGSAKETGLKLSEARDKAIDALRLL
AKGTDPREHRDVEKRRIQGSRQFGEFAEEWRRTYEIGMGHKAARAKLKRIVQVVCRPLHKHWVHEIETAHVVNVLMPIWQ
QPETSRSARQIIKKILDAAIAHNLRPKDNPADWASRLQPIMPRQRRRGTIRGGHKAMDYQDLPAFMHKLSAISTQSARAL
EVIILTLGRTAEVQNMRWAQLDLDNGVWDLGFLGTKNERLKRTPLPRQTLTYLREAYESRVNDEFVFPGRSLKRPMSNMT
MLKYLKELTGDDSLTVHGFRTTFRTWAQEQTHFEEEIVEHCLHHITGDDAGKGL
>Mature_374_residues
MQFLTQKPEITAKNYLSVGPGEYPCGNTLYLIVTPSGGRRWAFRYQRNGIVKKMGFGSAKETGLKLSEARDKAIDALRLL
AKGTDPREHRDVEKRRIQGSRQFGEFAEEWRRTYEIGMGHKAARAKLKRIVQVVCRPLHKHWVHEIETAHVVNVLMPIWQ
QPETSRSARQIIKKILDAAIAHNLRPKDNPADWASRLQPIMPRQRRRGTIRGGHKAMDYQDLPAFMHKLSAISTQSARAL
EVIILTLGRTAEVQNMRWAQLDLDNGVWDLGFLGTKNERLKRTPLPRQTLTYLREAYESRVNDEFVFPGRSLKRPMSNMT
MLKYLKELTGDDSLTVHGFRTTFRTWAQEQTHFEEEIVEHCLHHITGDDAGKGL

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=370, Percent_Identity=29.1891891891892, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788690, Length=369, Percent_Identity=25.4742547425474, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788974, Length=351, Percent_Identity=24.5014245014245, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 43040; Mature: 43040

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLTQKPEITAKNYLSVGPGEYPCGNTLYLIVTPSGGRRWAFRYQRNGIVKKMGFGSAK
CCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCCCCCCH
ETGLKLSEARDKAIDALRLLAKGTDPREHRDVEKRRIQGSRQFGEFAEEWRRTYEIGMGH
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
KAARAKLKRIVQVVCRPLHKHWVHEIETAHVVNVLMPIWQQPETSRSARQIIKKILDAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AHNLRPKDNPADWASRLQPIMPRQRRRGTIRGGHKAMDYQDLPAFMHKLSAISTQSARAL
HHCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
EVIILTLGRTAEVQNMRWAQLDLDNGVWDLGFLGTKNERLKRTPLPRQTLTYLREAYESR
HHHEEECCCCHHHCCCEEEEEECCCCCEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
VNDEFVFPGRSLKRPMSNMTMLKYLKELTGDDSLTVHGFRTTFRTWAQEQTHFEEEIVEH
CCCCEECCCHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
CLHHITGDDAGKGL
HHHHCCCCCCCCCC
>Mature Secondary Structure
MQFLTQKPEITAKNYLSVGPGEYPCGNTLYLIVTPSGGRRWAFRYQRNGIVKKMGFGSAK
CCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCCCCCCH
ETGLKLSEARDKAIDALRLLAKGTDPREHRDVEKRRIQGSRQFGEFAEEWRRTYEIGMGH
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
KAARAKLKRIVQVVCRPLHKHWVHEIETAHVVNVLMPIWQQPETSRSARQIIKKILDAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AHNLRPKDNPADWASRLQPIMPRQRRRGTIRGGHKAMDYQDLPAFMHKLSAISTQSARAL
HHCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
EVIILTLGRTAEVQNMRWAQLDLDNGVWDLGFLGTKNERLKRTPLPRQTLTYLREAYESR
HHHEEECCCCHHHCCCEEEEEECCCCCEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
VNDEFVFPGRSLKRPMSNMTMLKYLKELTGDDSLTVHGFRTTFRTWAQEQTHFEEEIVEH
CCCCEECCCHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
CLHHITGDDAGKGL
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]