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The map label for this gene is bigR [H]

Identifier: 146340158

GI number: 146340158

Start: 3379597

End: 3379947

Strand: Reverse

Name: bigR [H]

Synonym: BRADO3173

Alternate gene names: 146340158

Gene position: 3379947-3379597 (Counterclockwise)

Preceding gene: 146340159

Following gene: 146340157

Centisome position: 45.33

GC content: 62.39

Gene sequence:

>351_bases
ATGCCGCTGCCGAAGATGAAGGACATCGAAACCTCCGCCGAGCTCGAGCAGATGATCGAGAAGGCGCGCGAGGCCAGCGA
GATGCTGAAGGCCCTGTCCCACGAGTCCCGGCTGCTGCTGCTGTGCATTCTCTCCGAGGGCGAAAAATCCGTTACCGAGC
TCGAGCAGTTTCTCGGTGAGCGGCAGTCGACCGTCTCGCAGCAGCTCGCCCGTCTCAGGCTCGACCGGCTGGTAACGACG
CGGCGCGACGGCAAGACGATCTATTACAGTCTCGCCAGTGAGGACGTGCGCAAGATTCTGACGGCGATCTATGACGTCTT
CTGCGAGCCGGTCCGCAAGCGTCGTCGCTGA

Upstream 100 bases:

>100_bases
TCCGGGCGATGCGTTCTTCTTTGGGCTGCTCGGCCGCCTGATGAATGCTACAAGCGGGACGCCGGCCCGCTCCTGACCGA
CAATCGAGGATCCCAGTTTC

Downstream 100 bases:

>100_bases
CCATCGCTCTGCGCTGCGGCATCCTCGGACGCACGCGCGACAGGCAGGCGCGAACGCCGACAGCGGGTGTCGCAAGAGGT
CTGGCTTCGCCGTCGATCCT

Product: Ars family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MPLPKMKDIETSAELEQMIEKAREASEMLKALSHESRLLLLCILSEGEKSVTELEQFLGERQSTVSQQLARLRLDRLVTT
RRDGKTIYYSLASEDVRKILTAIYDVFCEPVRKRRR

Sequences:

>Translated_116_residues
MPLPKMKDIETSAELEQMIEKAREASEMLKALSHESRLLLLCILSEGEKSVTELEQFLGERQSTVSQQLARLRLDRLVTT
RRDGKTIYYSLASEDVRKILTAIYDVFCEPVRKRRR
>Mature_115_residues
PLPKMKDIETSAELEQMIEKAREASEMLKALSHESRLLLLCILSEGEKSVTELEQFLGERQSTVSQQLARLRLDRLVTTR
RDGKTIYYSLASEDVRKILTAIYDVFCEPVRKRRR

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=40.625, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 13486; Mature: 13355

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPKMKDIETSAELEQMIEKAREASEMLKALSHESRLLLLCILSEGEKSVTELEQFLGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
RQSTVSQQLARLRLDRLVTTRRDGKTIYYSLASEDVRKILTAIYDVFCEPVRKRRR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLPKMKDIETSAELEQMIEKAREASEMLKALSHESRLLLLCILSEGEKSVTELEQFLGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
RQSTVSQQLARLRLDRLVTTRRDGKTIYYSLASEDVRKILTAIYDVFCEPVRKRRR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]