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The map label for this gene is hsrA [H]

Identifier: 146340148

GI number: 146340148

Start: 3365624

End: 3367111

Strand: Reverse

Name: hsrA [H]

Synonym: BRADO3163

Alternate gene names: 146340148

Gene position: 3367111-3365624 (Counterclockwise)

Preceding gene: 146340157

Following gene: 146340147

Centisome position: 45.16

GC content: 66.47

Gene sequence:

>1488_bases
ATGAACAAGCAACGCATCATTCCGCTGATCGTCGCCACCGCCCTGTTCATGGAGAACATGGACTCCACGGTCATCGCGAC
CTCGCTGCCGGCGATCGCTAGGGATATCGGCACCAGCCCGCTGACCTTGAAGCTCGCGATCACGTCCTACCTGCTGTCGC
TCGCGGTGTTCATCCCGGCGAGCGGCTGGACGGCCGACCGCTTCGGCGCACGGCGGGTGTTCTCCAGCGCGGTCGCGGTG
TTCATGATCGGCTCGATCGGGTGCGCGCTCGCCAACTCGGTCGAGAACTTCGTGTTCGCGCGCATCCTGCAGGGCATGGG
CGGGGCGATGATGACGCCGGTCGGCCGCCTCGTGCTGCTGCGCTCCGTCGACAAGAGCGCGCTGGTCGGCGCGATGGCCT
GGGTCACGATCCCCGCGCTGATCGGCCCGGTGATCGGGCCGCCGGTCGGCGGCTTCATCACCACCTATTTCTCCTGGCAC
TGGATCTTCCTGATCAACATCCCGATCGGCATCGTCGGCATCATCATGGCGCTGCGCTTCATCGATCCGATCAAGAGTGA
CAATCCAGAGCCGTTCGATCTCTACGGCATGGTGCTCGCCGGCATCGGGCTGGCCGGCCTCGCCTTCGGCCTGTCGGTCG
CCGGCCTCAATCTGCTGCCGTGGTCGGTGGTCGGCAGCCTGGTCGCGATCGGCGCGATCTCGATGACGCTCTATGTCCGC
CACTCCTGGCGGACCAATTCGCCGGTGCTCGACTTCTCGCTGCTGCGGCTGCCGACCTTGCGGGCCTCGATCGTCGGCGG
CTTCATGTTCCGGCTCGGCATCGGCGCCCTGCCCTTCCTGCTGCCGCTGTTGATGCAGCTCGGCTTCGGCCTGTCGCCGT
TCCAGTCCGGCCTCGTCACCTTCGGCTCCTCGGCCGGCGCGATGGGCATGAAGGCGCTGGCGGCGCGGCTGATCAAGACC
TTCGGCTTCCGCCACCTCATGACGGTCAATGCGGTCGTCAGCTCGGTCTTCCTCGCCGCCTGCGCGCTGTTCACGCCGGC
GACGCCGCTGTTGCTGATCGTCATCATCCTGTTCGTCGGCGGCTTCTTCCGCTCGCTGCAGTTCACCGCGATCAACACCG
TCGCCTATGCCGAGGTCGAGACCGCGCAGATGAGCCGCGCCACCACGCTGACCAGCGTCAACCAGCAGCTCGCGATCTCC
GCCGGAGTCGCCGTCGGCGCGTTTTCAGTGGAGACGACGATGTGGGCTCATGGTGCGAGCCAGCTGACTGCCACCTCCTT
CGCGCCAGCCTTCATCGTCGTCGCCATCACCTCGGCGCTGTCGTCGCTCCTGTTCTGGCAGATGCCCGACGATGCCGGCA
GCGAAATCTCCGGCCGGAAGGTCAATGCGATCGCGAGCCGCAAGGGCGCGGAAAAAGGCGCCGAGAAGGCCGCGACCAAG
TCGGCCAGCGAGACCACGCACGACGCGCGGGATCAGAAGCTGGGATGA

Upstream 100 bases:

>100_bases
GCCACCCTGCATTTGGGGGGCCATGCCGCCCACGCAATGGCCCTGTCCGTCGCAGGGTGTTAAAGCCGCCGCGCCCGCCG
CTTTCCCTCCCCAGGATTCG

Downstream 100 bases:

>100_bases
TAGGCCCCTCCCGTCATCGCGAGCGCAGCGAAGCGATCCACAGTGATGGGCGGGACTCTGGATTGCTTCGCTGCGCTCGC
AATGACATGCTGAGGGATAG

Product: putative multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNKQRIIPLIVATALFMENMDSTVIATSLPAIARDIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARRVFSSAVAV
FMIGSIGCALANSVENFVFARILQGMGGAMMTPVGRLVLLRSVDKSALVGAMAWVTIPALIGPVIGPPVGGFITTYFSWH
WIFLINIPIGIVGIIMALRFIDPIKSDNPEPFDLYGMVLAGIGLAGLAFGLSVAGLNLLPWSVVGSLVAIGAISMTLYVR
HSWRTNSPVLDFSLLRLPTLRASIVGGFMFRLGIGALPFLLPLLMQLGFGLSPFQSGLVTFGSSAGAMGMKALAARLIKT
FGFRHLMTVNAVVSSVFLAACALFTPATPLLLIVIILFVGGFFRSLQFTAINTVAYAEVETAQMSRATTLTSVNQQLAIS
AGVAVGAFSVETTMWAHGASQLTATSFAPAFIVVAITSALSSLLFWQMPDDAGSEISGRKVNAIASRKGAEKGAEKAATK
SASETTHDARDQKLG

Sequences:

>Translated_495_residues
MNKQRIIPLIVATALFMENMDSTVIATSLPAIARDIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARRVFSSAVAV
FMIGSIGCALANSVENFVFARILQGMGGAMMTPVGRLVLLRSVDKSALVGAMAWVTIPALIGPVIGPPVGGFITTYFSWH
WIFLINIPIGIVGIIMALRFIDPIKSDNPEPFDLYGMVLAGIGLAGLAFGLSVAGLNLLPWSVVGSLVAIGAISMTLYVR
HSWRTNSPVLDFSLLRLPTLRASIVGGFMFRLGIGALPFLLPLLMQLGFGLSPFQSGLVTFGSSAGAMGMKALAARLIKT
FGFRHLMTVNAVVSSVFLAACALFTPATPLLLIVIILFVGGFFRSLQFTAINTVAYAEVETAQMSRATTLTSVNQQLAIS
AGVAVGAFSVETTMWAHGASQLTATSFAPAFIVVAITSALSSLLFWQMPDDAGSEISGRKVNAIASRKGAEKGAEKAATK
SASETTHDARDQKLG
>Mature_495_residues
MNKQRIIPLIVATALFMENMDSTVIATSLPAIARDIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARRVFSSAVAV
FMIGSIGCALANSVENFVFARILQGMGGAMMTPVGRLVLLRSVDKSALVGAMAWVTIPALIGPVIGPPVGGFITTYFSWH
WIFLINIPIGIVGIIMALRFIDPIKSDNPEPFDLYGMVLAGIGLAGLAFGLSVAGLNLLPWSVVGSLVAIGAISMTLYVR
HSWRTNSPVLDFSLLRLPTLRASIVGGFMFRLGIGALPFLLPLLMQLGFGLSPFQSGLVTFGSSAGAMGMKALAARLIKT
FGFRHLMTVNAVVSSVFLAACALFTPATPLLLIVIILFVGGFFRSLQFTAINTVAYAEVETAQMSRATTLTSVNQQLAIS
AGVAVGAFSVETTMWAHGASQLTATSFAPAFIVVAITSALSSLLFWQMPDDAGSEISGRKVNAIASRKGAEKGAEKAATK
SASETTHDARDQKLG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=36.8085106382979, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788392, Length=466, Percent_Identity=36.2660944206009, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1788710, Length=432, Percent_Identity=27.7777777777778, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1789042, Length=428, Percent_Identity=25, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87081983, Length=355, Percent_Identity=28.4507042253521, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790146, Length=168, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=253, Percent_Identity=26.8774703557312, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52173; Mature: 52173

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQRIIPLIVATALFMENMDSTVIATSLPAIARDIGTSPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARRVFSSAVAVFMIGSIGCALANSVENFVFARILQGMGGAMMTPVGRLVLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSVDKSALVGAMAWVTIPALIGPVIGPPVGGFITTYFSWHWIFLINIPIGIVGIIMALRF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
IDPIKSDNPEPFDLYGMVLAGIGLAGLAFGLSVAGLNLLPWSVVGSLVAIGAISMTLYVR
HCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEE
HSWRTNSPVLDFSLLRLPTLRASIVGGFMFRLGIGALPFLLPLLMQLGFGLSPFQSGLVT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
FGSSAGAMGMKALAARLIKTFGFRHLMTVNAVVSSVFLAACALFTPATPLLLIVIILFVG
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTAINTVAYAEVETAQMSRATTLTSVNQQLAISAGVAVGAFSVETTMWAHGAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCC
QLTATSFAPAFIVVAITSALSSLLFWQMPDDAGSEISGRKVNAIASRKGAEKGAEKAATK
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHC
SASETTHDARDQKLG
CCHHHHHHHHHCCCC
>Mature Secondary Structure
MNKQRIIPLIVATALFMENMDSTVIATSLPAIARDIGTSPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARRVFSSAVAVFMIGSIGCALANSVENFVFARILQGMGGAMMTPVGRLVLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSVDKSALVGAMAWVTIPALIGPVIGPPVGGFITTYFSWHWIFLINIPIGIVGIIMALRF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
IDPIKSDNPEPFDLYGMVLAGIGLAGLAFGLSVAGLNLLPWSVVGSLVAIGAISMTLYVR
HCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEE
HSWRTNSPVLDFSLLRLPTLRASIVGGFMFRLGIGALPFLLPLLMQLGFGLSPFQSGLVT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
FGSSAGAMGMKALAARLIKTFGFRHLMTVNAVVSSVFLAACALFTPATPLLLIVIILFVG
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTAINTVAYAEVETAQMSRATTLTSVNQQLAISAGVAVGAFSVETTMWAHGAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCC
QLTATSFAPAFIVVAITSALSSLLFWQMPDDAGSEISGRKVNAIASRKGAEKGAEKAATK
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHC
SASETTHDARDQKLG
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]